Term IRI Term label Parent term IRI Parent term label Alternative term Definition http://purl.obolibrary.org/obo/ECO_0006151 documented statement evidence http://purl.obolibrary.org/obo/ECO_0000000 evidence A type of evidence in which information is recorded in some documentation system, for example, but not limited to a publication, survey, or medical record. http://purl.obolibrary.org/obo/ECO_0006152 medical practitioner statement evidence http://purl.obolibrary.org/obo/ECO_0006151 documented statement evidence A type of documented statement evidence in which information is stated by a health care professional, for example, but not limited to a doctor, nurse, or psychologist. http://purl.obolibrary.org/obo/ECO_0006153 self-reported individual's statement evidence http://purl.obolibrary.org/obo/ECO_0006151 documented statement evidence A type of documented statement evidence in which information is provided by an individual through means including, but not limited to, paper form, electronic form, or verbal communication, and is captured in a documented record. http://purl.obolibrary.org/obo/ECO_0006154 self-reported patient statement evidence http://purl.obolibrary.org/obo/ECO_0006153 self-reported individual's statement evidence A type of self-reported individual's statement evidence in which information is provided by a patient in a clinical setting. http://purl.obolibrary.org/obo/ECO_0006155 documented statement evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000352 evidence used in manual assertion A type of documented statement evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006156 documented statement evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000501 evidence used in automatic assertion A type of documented statement evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006157 self-reported individual's statement evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006155 documented statement evidence used in manual assertion A type of self-reported individual's statement evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006158 self-reported individual's statement evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006156 documented statement evidence used in automatic assertion A type of self-reported individual's statement evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006159 self-reported patient statement evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006157 self-reported individual's statement evidence used in manual assertion A type of self-reported patient statement evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006160 self-reported patient statement evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006158 self-reported individual's statement evidence used in automatic assertion A type of self-reported patient statement evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006161 medical practitioner statement evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006155 documented statement evidence used in manual assertion A type of medical practitioner statement evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006162 medical practitioner statement evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006156 documented statement evidence used in automatic assertion A type of medical practitioner statement evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/CHEBI_23367 molecular entity http://purl.obolibrary.org/obo/BFO_0000040 material entity Any constitutionally or isotopically distinct atom, molecule, ion, ion pair, radical, radical ion, complex, conformer etc., identifiable as a separately distinguishable entity. http://purl.obolibrary.org/obo/CHEBI_28619 acrylamide http://purl.obolibrary.org/obo/CHEBI_23367 molecular entity A member of the class of acrylamides that results from the formal condensation of acrylic acid with ammonia. http://purl.obolibrary.org/obo/CHEBI_33250 atom http://purl.obolibrary.org/obo/BFO_0000040 material entity A chemical entity constituting the smallest component of an element having the chemical properties of the element. http://purl.obolibrary.org/obo/CHEBI_50076 chromium-51 http://purl.obolibrary.org/obo/CHEBI_23367 molecular entity A synthetic radioactive isotope of chromium having a half-life of 27.7 days and decaying by electron capture with emission of gamma rays (0.32 MeV); it is used to label red blood cells for measurement of mass or volume, survival time, and sequestration studies, for the diagnosis of gastrointestinal bleeding, and to label platelets to study their survival. http://purl.obolibrary.org/obo/ECO_0006398 antibody Tu36-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody Tu36 to determine that the HLA-DR protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0006399 antibody Tu36-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody Tu36-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006400 antibody Tu36-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody Tu36-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005632 fluorescence anisotropy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001811 fluorescence anisotropy evidence A type of fluorescence anisotropy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/PATO_0000169 viability http://purl.obolibrary.org/obo/PATO_0001995 organismal quality An organismal quality inhering in a bearer or a population by virtue of the bearer's disposition to survive and develop normally or the number of surviving individuals in a given population. http://purl.obolibrary.org/obo/PATO_0001018 physical quality http://purl.obolibrary.org/obo/PATO_0001241 physical object quality A quality of a physical entity that exists through action of continuants at the physical level of organisation in relation to other entities. http://purl.obolibrary.org/obo/PATO_0001241 physical object quality http://purl.obolibrary.org/obo/BFO_0000019 quality A quality which inheres in a continuant. http://purl.obolibrary.org/obo/PATO_0001291 electromagnetic (EM) radiation quality http://purl.obolibrary.org/obo/PATO_0001739 radiation quality A physical quality that inheres in an bearer by virtue of how that bearer interacts with electromagnetic radiation. http://purl.obolibrary.org/obo/PATO_0001296 luminous flux http://purl.obolibrary.org/obo/PATO_0001300 optical quality A scalar optical quality which obtains by the magnitude of the light emitted by the bearer. http://purl.obolibrary.org/obo/PATO_0001300 optical quality http://purl.obolibrary.org/obo/PATO_0001291 electromagnetic (EM) radiation quality An EM radiation quality in which the EM radiation is within the fiat range of the spectrum visible deemed to be light. http://purl.obolibrary.org/obo/PATO_0001739 radiation quality http://purl.obolibrary.org/obo/PATO_0001018 physical quality A quality that inheres in an bearer by virtue of how that bearer interacts with radiation. http://purl.obolibrary.org/obo/PATO_0001740 activity (of a radionuclide) http://purl.obolibrary.org/obo/PATO_0001739 radiation quality A radiation quality inhering in a radioactive substance by virtue of its transformation (disintegration) rate. http://purl.obolibrary.org/obo/OBI_0002165 protein localization assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that determines the specific location of a protein. Subcellular localization is distinguished from tissue-based localization based on the type of microscopy applied. http://purl.obolibrary.org/obo/OBI_0002167 subcellular protein localization assay http://purl.obolibrary.org/obo/OBI_0002165 protein localization assay A protein localization assay that determines the specific subcellular location of a protein. The location is visualized through electron microscopy. http://purl.obolibrary.org/obo/OBI_0002169 ChIP-qPCR assay http://purl.obolibrary.org/obo/OBI_0002692 polymerase chain reaction assay A ChIP assay that uses quantitative PCR to determine levels of specific DNA in immunoprecipitated samples. http://purl.obolibrary.org/obo/OBI_0002171 dot blot assay http://purl.obolibrary.org/obo/OBI_0000443 analyte assay An analyte assay that detects molecules in a mixture dotted on a membrane using DNA probes or antibodies. http://purl.obolibrary.org/obo/OBI_0002175 ATP bioluminescence assay http://purl.obolibrary.org/obo/OBI_0000443 analyte assay An analyte assay that detects ATP concentration through light intensity when luciferase catalyzes the oxidation of luciferin in the presence of ATP, magnesium ions, and molecular oxygen. http://purl.obolibrary.org/obo/OBI_0002176 electrophysiology assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that measures the electrical properties of biological cells or tissues. Typically, this assay will generate measurements of voltage changes or electric current (or other associated variables such as impedence or capacitance). http://purl.obolibrary.org/obo/OBI_0002177 patch clamp assay http://purl.obolibrary.org/obo/OBI_0000447 intra cellular electrophysiology recording assay An intracellular electrophysiology assay where a glass micropipette is sealed to the surface of the cell membrane as a recording electrode to study ion channel activity. The key distinction of this technique is the electrical resistance of the seal between the cell membrane and the pipette is of the order 10-100 gigaohms, permitting high-resolution current measurements over the cell membrane in several different standard configurations. http://purl.obolibrary.org/obo/OBI_0002178 whole-cell patch clamp assay http://purl.obolibrary.org/obo/OBI_0002177 patch clamp assay A patch-clamp assay where the electrode is left in place on the cell, as in cell-attached recordings, but the membrane patch has been perforated, providing access from the interior of the pipette to the intracellular space of the cell. Measurements made with this technique involve recording currents through multiple channels simultaneously, over the membrane of the entire cell. http://purl.obolibrary.org/obo/OBI_0002179 cell-attached patch clamp assay http://purl.obolibrary.org/obo/OBI_0002177 patch clamp assay A patch-clamp assay where the electrode is left in place on the cell, and the membrane patch has been left intact been perforated. This maintains the separation of the interior of the pipette to the intracellular space of the cell. Measurements made with this technique involve recording currents through multiple channels simultaneously, over the membrane of the entire cell. http://purl.obolibrary.org/obo/OBI_0002180 inside-out patch clamp assay http://purl.obolibrary.org/obo/OBI_0002177 patch clamp assay A patch-clamp assay where a patch of the membrane is attached to the patch pipette, detached from the rest of the cell, and the cytosolic surface of the membrane is exposed to the external media, or bath. This provides the experimenter has access to the intracellular surface of the membrane via the bath and can manipulate the environment at the intracellular surface of single ion channels. For example, channels that are activated by intracellular ligands can then be studied through a range of ligand concentrations. http://purl.obolibrary.org/obo/OBI_0002181 outside-out patch clamp assay http://purl.obolibrary.org/obo/OBI_0002177 patch clamp assay A patch-clamp assay where a patch of the membrane is attached to the patch pipette. In this configuration, the external surface of the cell membrane is exposed as the outside of the membrane patch relative to the patch electrode. An outside-out patch starts with a gigaohm seal in a whole-cell recording configuration. The electrode is slowly withdrawn from the cell, until a fragment of membrane bulges away from the cell, which detaches and reforms as a convex membrane on the end of the electrode, with the original external surface of the membrane facing outward from the electrode. This provides the experimenter with access to the extracellular surface of the membrane via the bath and can manipulate the environment at the extracellular surface of single ion channels. http://purl.obolibrary.org/obo/OBI_0002186 electroencephalography http://purl.obolibrary.org/obo/OBI_0000454 extracellular electrophysiology recording assay An extracellular electrophysiology assay where electrodes are mounted outside the brain (either on the surface of the scalp on onto the brain surface itself during surgery) to measure the electrical field over the external surface. http://purl.obolibrary.org/obo/OBI_0002187 single-unit recording http://purl.obolibrary.org/obo/OBI_0001977 cytometry assay An extracellular electrophysiology assay where a single microelectrode is placed in close proximity to a single neuron to measure voltage and current changes over time. This is the technicque used by Hubel and Wiesel to measure firing properties of primary visual cortex neurons in the 1950s in their original Nobel-prize winning study. A classic, old technique. http://purl.obolibrary.org/obo/OBI_0002189 local field potential recording http://purl.obolibrary.org/obo/OBI_0000454 extracellular electrophysiology recording assay An extracellular electrophysiology assay where a microelectrode is placed in the extracellular space of brain tissue to measure action potential and compared to an electrode either outside or inside that tissue. http://purl.obolibrary.org/obo/ECO_0007666 automatically integrated combinatorial computational and experimental evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000245 automatically integrated combinatorial evidence used in manual assertion A type of automatically integrated combinatorial computational and experimental evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001574 membrane fusion assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence in which a broad variety of physiological processes are measured when two separate membranes merge into a single contiguous membrane. http://purl.obolibrary.org/obo/ECO_0001575 membrane fusion assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of membrane fusion assay evidence that is used in automatic assertion. http://purl.obolibrary.org/obo/ECO_0001576 membrane fusion assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of membrane fusion assay evidence that is used in manual assertion. http://purl.obolibrary.org/obo/ECO_0001577 spheroplast fusion assay evidence http://purl.obolibrary.org/obo/ECO_0001574 membrane fusion assay evidence A type of membrane fusion assay evidence based on the fusion of spheroplasts. http://purl.obolibrary.org/obo/ECO_0001578 spheroplast fusion assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001577 spheroplast fusion assay evidence A type of spheroplast fusion assay evidence used in automatic assertion. http://purl.obolibrary.org/obo/ECO_0001579 spheroplast fusion assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001577 spheroplast fusion assay evidence A type of spheroplast fusion assay evidence that is used in manual assertion. http://purl.obolibrary.org/obo/ECO_0001580 liquid chromatography coupled with tandem mass spectrometry evidence http://purl.obolibrary.org/obo/ECO_0001096 mass spectrometry evidence A type of mass spectrometry evidence where liquid chromatography is used to separate particles in solution, followed by fragmentation and measurement of the mass-to-charge ratio of the resulting particles to identify the amount and type of material entities present in a sample. http://purl.obolibrary.org/obo/ECO_0001581 liquid chromatography coupled with tandem mass spectrometry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007433 mass spectrometry evidence used in automatic assertion A type of liquid chromatography coupled with tandem mass spectrometry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0001582 liquid chromatography coupled with tandem mass spectrometry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001230 mass spectrometry evidence used in manual assertion A type of liquid chromatography coupled with tandem mass spectrometry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001583 small interfering RNA knockdown evidence http://purl.obolibrary.org/obo/ECO_0000018 anti-sense experiment evidence A type of anti-sense experiment evidence where gene expression is disrupted through the introduction of double-stranded RNA molecules, 20-25 base pairs in length, which operate within the RNA interference pathway. http://purl.obolibrary.org/obo/ECO_0001584 small interfering RNA knockdown evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007556 anti-sense experiment evidence used in automatic assertion A type of small interfering RNA knockdown evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0001585 small interfering RNA knockdown evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006050 anti-sense experiment evidence used in manual assertion A type of small interfering RNA knockdown evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001586 ion trap mass spectrometry evidence http://purl.obolibrary.org/obo/ECO_0001096 mass spectrometry evidence A type of mass spectrometry evidence where a combination of magnetic and/or electric fields are utilized to capture charged particles in tandem with mass spectrometry. http://purl.obolibrary.org/obo/ECO_0001587 ion trap mass spectrometry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007433 mass spectrometry evidence used in automatic assertion A type of ion trap mass spectrometry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0001588 ion trap mass spectrometry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001230 mass spectrometry evidence used in manual assertion A type of ion trap mass spectrometry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001589 atomic force microscopy evidence http://purl.obolibrary.org/obo/ECO_0001098 microscopy evidence A type of microscopy evidence where a mechanical probe is applied to a sample (i.e. a type of scanning probe microscopy) which can result in production of an image (with resolution in the order of fractions of a nanometer), force measurement, or sample manipulation. http://purl.obolibrary.org/obo/ECO_0001590 atomic force microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007435 microscopy evidence used in automatic assertion A type of atomic force microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0001591 atomic force microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001232 microscopy evidence used in manual assertion A type of atomic force microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001592 polysome profiling evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence employed to study the associations between multiple ribosomes and mRNA that utilizes density gradient centrifugation to separate out the lysate of cells of interest with the objective of isolating polysomes/polyribosomes which are complexes of an mRNA molecule and two or more ribosomes that act to translate mRNA. The optical density of the fractions is then determined. http://purl.obolibrary.org/obo/ECO_0001593 polysome profiling evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of polysome profiling evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0001594 polysome profiling evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of polysome profiling evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001598 multilocus sequence typing evidence http://purl.obolibrary.org/obo/ECO_0000219 nucleotide sequencing assay evidence A type of nucleotide sequencing assay evidence in which DNA sequence variations within a specific set of genes (typically housekeeping genes) are used to characterizes strains by their unique allelic profiles. http://purl.obolibrary.org/obo/ECO_0001599 multilocus sequence typing evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007813 nucleotide sequencing assay evidence used in automatic assertion A type of multilocus sequence typing evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0001600 multilocus sequence typing evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of multilocus sequence typing evidence that is used in a manual assertion http://purl.obolibrary.org/obo/ECO_0001830 host colonization assay evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence in which the colonization or adhesion capacity is measured. http://purl.obolibrary.org/obo/ECO_0001831 host colonization assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of host colonization assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0001832 host colonization assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of host colonization assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001833 infection assay evidence http://purl.obolibrary.org/obo/ECO_0001830 host colonization assay evidence A type of host colonization assay evidence in which the infection capacity is measured by assessing changes in the host. http://purl.obolibrary.org/obo/ECO_0001834 infection assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001833 infection assay evidence A type of infection assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0001835 infection assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001833 infection assay evidence A type of infection assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007682 reporter gene assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000270 expression pattern evidence used in manual assertion A type of reporter gene assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007684 protein separation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006005 fractionation evidence used in manual assertion A type of protein separation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007685 substance quantification evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000335 substance quantification evidence A type of substance quantification evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007686 voltage clamp recording evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006006 electrophysiology assay evidence used in manual assertion A type of voltage clamp recording evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007687 protein kinase assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001558 protein kinase assay evidence A type of protein kinase assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007688 gel electrophoresis evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000337 gel electrophoresis evidence A type of gel electrophoresis evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007689 sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000333 sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence A type of sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007690 ex vivo assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005502 ex vivo assay evidence A type of ex vivo assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007691 cleavage assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001547 cleavage assay evidence A type of cleavage assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007692 deacetylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001548 deacetylation assay evidence A type of deacetylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007693 transcription assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of transcription assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007694 phosphatase assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001556 phosphatase assay evidence A type of phosphatase assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007695 cell-based assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001565 cell-based assay evidence A type of cell-based assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007696 cell proliferation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005007 cell proliferation assay evidence A type of cell proliferation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007697 DNA synthesis cell proliferation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005008 DNA synthesis cell proliferation assay evidence A type of DNA synthesis cell proliferation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007698 apoptotic assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005034 apoptotic assay evidence A type of apoptotic assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007699 cell growth assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001563 cell growth assay evidence A type of cell growth assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007700 disk diffusion test evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007084 zone of inhibition evidence used in manual assertion A type of disk diffusion test evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007701 chemotaxis assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005021 chemotaxis assay evidence A type of chemotaxis assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007702 cytotoxicity assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005012 cytotoxicity assay evidence A type of cytotoxicity assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007703 cell viability assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005004 cell viability assay evidence A type of cell viability assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007704 methylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001554 methylation assay evidence A type of methylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007706 chromatin immunoprecipitation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005644 immunoprecipitation evidence used in manual assertion A type of chromatin immunoprecipitation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007707 protein inhibition evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of protein inhibition evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007708 sumoylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001559 sumoylation assay evidence A type of sumoylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007709 transport assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of transport assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007710 defarnesylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001549 defarnesylation assay evidence A type of defarnesylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007711 deubiquitination assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001552 deubiquitination assay evidence A type of deubiquitination assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007712 palmitoylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001555 palmitoylation assay evidence A type of palmitoylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007713 acetylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001546 acetylation assay evidence A type of acetylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007714 demethylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001550 demethylation assay evidence A type of demethylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007715 polyADP-ribosylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001557 polyADP-ribosylation assay evidence A type of polyADP-ribosylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007716 staining evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005019 staining evidence A type of staining evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007717 translation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001561 translation assay evidence A type of translation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007718 ubiquitination assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001562 ubiquitination assay evidence A type of ubiquitination assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007719 immunodetection assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005593 immunodetection assay evidence A type of immunodetection assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007720 desumoylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001551 desumoylation assay evidence A type of desumoylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007721 farnesylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001553 farnesylation assay evidence A type of farnesylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007722 reconstitution assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of reconstitution assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007723 in vitro transcription reconstitution assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of in vitro transcription reconstitution assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007724 localization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001533 localization evidence A type of localization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007725 protein localization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of protein localization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007726 fusion protein localization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of fusion protein localization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007727 nucleic acid localization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001534 nucleic acid localization evidence A type of nucleic acid localization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007728 anatomical perturbation phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000240 anatomical perturbation phenotypic evidence A type of anatomical perturbation phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007730 cleavage arrested development evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000298 cleavage arrested development evidence A type of cleavage arrested development evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007731 spectrometry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005504 spectrometry evidence A type of spectrometry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007732 sequencing assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of sequencing assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007733 nucleotide sequencing assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of nucleotide sequencing assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007734 high throughput nucleotide sequencing assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of high throughput nucleotide sequencing assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007735 structure determination evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005031 structure determination evidence A type of structure determination evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007736 molecule detection assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005516 molecule detection assay evidence A type of molecule detection assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007737 DNA detection assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005519 DNA detection assay evidence A type of DNA detection assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007738 protein detection assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005517 protein detection assay evidence A type of protein detection assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007739 RNA detection assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005518 RNA detection assay evidence A type of RNA detection assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007740 small molecule detection assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001522 small molecule detection assay evidence A type of small molecule detection assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007741 chromosome conformation-based evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of chromosome conformation-based evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007742 3C evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of 3C evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007744 combinatorial computational and experimental evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000244 combinatorial evidence used in manual assertion A type of combinatorial computational and experimental evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007745 combinatorial experimental evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000244 combinatorial evidence used in manual assertion A type of combinatorial experimental evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007746 biological system reconstruction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000305 curator inference used in manual assertion A type of biological system reconstruction evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007747 biological system reconstruction evidence by experimental evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005551 biological system reconstruction evidence by experimental evidence A type of biological system reconstruction evidence by experimental evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007748 phenotypic similarity evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000252 similarity evidence used in manual assertion A type of phenotypic similarity evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007749 transcript splice pattern evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000326 transcript splice pattern evidence A type of transcript splice pattern evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007750 phylogenetic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000252 similarity evidence used in manual assertion A type of phylogenetic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007751 inferential evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000361 inferential evidence A type of inferential evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007752 curator inference from authoritative source used in manual assertion http://purl.obolibrary.org/obo/ECO_0007647 curator inference from authoritative source A type of curator inference from authoritative source that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007753 curator inference from encyclopedia used in manual assertion http://purl.obolibrary.org/obo/ECO_0007637 curator inference from encyclopedia A type of curator inference from encyclopedia that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007754 curator inference from Wikipedia used in manual assertion http://purl.obolibrary.org/obo/ECO_0007638 curator inference from Wikipedia A type of curator inference from Wikipedia that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007755 curator inference from MedlinePlus encyclopedia used in manual assertion http://purl.obolibrary.org/obo/ECO_0007640 curator inference from MedlinePlus encyclopedia A type of curator inference from MedlinePlus encyclopedia that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007756 curator inference from Britannica used in manual assertion http://purl.obolibrary.org/obo/ECO_0007639 curator inference from Britannica A type of curator inference from Britannica that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007757 curator inference from book used in manual assertion http://purl.obolibrary.org/obo/ECO_0007646 curator inference from book A type of curator inference from book that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007758 curator inference from journal publication used in manual assertion http://purl.obolibrary.org/obo/ECO_0007645 curator inference from journal publication A type of curator inference from journal publication that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007759 curator inference from database used in manual assertion http://purl.obolibrary.org/obo/ECO_0007636 curator inference from database A type of curator inference from database that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007760 curator inference from dictionary used in manual assertion http://purl.obolibrary.org/obo/ECO_0007641 curator inference from dictionary A type of curator inference from dictionary that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007761 curator inference from MedlinePlus dictionary used in manual assertion http://purl.obolibrary.org/obo/ECO_0007644 curator inference from MedlinePlus dictionary A type of curator inference from MedlinePlus dictionary that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007762 curator inference from Oxford Dictionary used in manual assertion http://purl.obolibrary.org/obo/ECO_0007642 curator inference from Oxford Dictionary A type of curator inference from Oxford Dictionary that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007763 curator inference from Merriam-Webster Dictionary used in manual assertion http://purl.obolibrary.org/obo/ECO_0007643 curator inference from Merriam-Webster Dictionary A type of curator inference from Merriam-Webster Dictionary that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007765 reporter gene assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007298 expression pattern evidence used in automatic assertion A type of reporter gene assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007766 voltage clamp recording evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007535 electrophysiology assay evidence used in automatic assertion A type of voltage clamp recording evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007767 protein kinase assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001558 protein kinase assay evidence A type of protein kinase assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007768 transcription assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of transcription assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007769 gel electrophoresis evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of gel electrophoresis evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007770 sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000333 sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence A type of sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007771 deacetylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001548 deacetylation assay evidence A type of deacetylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007772 phosphatase assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001556 phosphatase assay evidence A type of phosphatase assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007773 cell-based assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001565 cell-based assay evidence A type of cell-based assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007774 cell viability assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005004 cell viability assay evidence A type of cell viability assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007775 cell proliferation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005007 cell proliferation assay evidence A type of cell proliferation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007776 DNA synthesis cell proliferation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005008 DNA synthesis cell proliferation assay evidence A type of DNA synthesis cell proliferation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007777 apoptotic assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005034 apoptotic assay evidence A type of apoptotic assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007778 cell growth assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001563 cell growth assay evidence A type of cell growth assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007779 disk diffusion test evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007628 zone of inhibition evidence used in automatic assertion A type of disk diffusion test evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007780 chemotaxis assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005021 chemotaxis assay evidence A type of chemotaxis assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007781 cytotoxicity assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005012 cytotoxicity assay evidence A type of cytotoxicity assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007782 cleavage assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001547 cleavage assay evidence A type of cleavage assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007783 methylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001554 methylation assay evidence A type of methylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007785 protein inhibition evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of protein inhibition evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007786 chromatin immunoprecipitation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007503 immunoprecipitation evidence used in automatic assertion A type of chromatin immunoprecipitation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007787 protein separation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007534 fractionation evidence used in automatic assertion A type of protein separation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007788 sumoylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001559 sumoylation assay evidence A type of sumoylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007789 transport assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of transport assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007790 defarnesylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001549 defarnesylation assay evidence A type of defarnesylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007791 deubiquitination assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001552 deubiquitination assay evidence A type of deubiquitination assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007792 palmitoylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001555 palmitoylation assay evidence A type of palmitoylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007793 ex vivo assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of ex vivo assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007794 acetylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001546 acetylation assay evidence A type of acetylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007795 demethylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001550 demethylation assay evidence A type of demethylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007796 immunodetection assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of immunodetection assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007797 polyADP-ribosylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001557 polyADP-ribosylation assay evidence A type of polyADP-ribosylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007798 staining evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of staining evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007799 translation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001561 translation assay evidence A type of translation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007800 farnesylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001553 farnesylation assay evidence A type of farnesylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007801 ubiquitination assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001562 ubiquitination assay evidence A type of ubiquitination assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007802 desumoylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001551 desumoylation assay evidence A type of desumoylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007803 reconstitution assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of reconstitution assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007804 in vitro transcription reconstitution assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000150 in vitro transcription reconstitution assay evidence A type of in vitro transcription reconstitution assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007805 substance quantification evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of substance quantification evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007806 localization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of localization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007807 protein localization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000122 protein localization evidence A type of protein localization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007808 fusion protein localization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000124 fusion protein localization evidence A type of fusion protein localization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007809 nucleic acid localization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001534 nucleic acid localization evidence A type of nucleic acid localization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007810 anatomical perturbation phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000240 anatomical perturbation phenotypic evidence A type of anatomical perturbation phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007811 cleavage arrested development evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000298 cleavage arrested development evidence A type of cleavage arrested development evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007812 sequencing assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of sequencing assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007813 nucleotide sequencing assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000219 nucleotide sequencing assay evidence A type of nucleotide sequencing assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007814 high throughput nucleotide sequencing assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000221 high throughput nucleotide sequencing assay evidence A type of high throughput nucleotide sequencing assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007815 structure determination evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of structure determination evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007816 molecule detection assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of molecule detection assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007817 DNA detection assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005519 DNA detection assay evidence A type of DNA detection assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007818 RNA detection assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005518 RNA detection assay evidence A type of RNA detection assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007819 protein detection assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005517 protein detection assay evidence A type of protein detection assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007820 small molecule detection assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001522 small molecule detection assay evidence A type of small molecule detection assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007821 spectrometry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of spectrometry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007822 chromosome conformation-based evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of chromosome conformation-based evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007823 3C evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000233 3C evidence A type of 3C evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007824 phenotypic similarity evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000251 similarity evidence used in automatic assertion A type of phenotypic similarity evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007825 transcript splice pattern evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000326 transcript splice pattern evidence A type of transcript splice pattern evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007826 phylogenetic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000251 similarity evidence used in automatic assertion A type of phylogenetic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007827 biological system reconstruction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007322 curator inference used in automatic assertion A type of biological system reconstruction evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007828 biological system reconstruction evidence by experimental evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005551 biological system reconstruction evidence by experimental evidence A type of biological system reconstruction evidence by experimental evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007829 combinatorial computational and experimental evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000213 combinatorial evidence used in automatic assertion A type of combinatorial computational and experimental evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007830 combinatorial experimental evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000213 combinatorial evidence used in automatic assertion A type of combinatorial experimental evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007832 inferential evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000501 evidence used in automatic assertion A type of inferential evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007833 curator inference from authoritative source used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007647 curator inference from authoritative source A type of curator inference from authoritative source that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007834 curator inference from encyclopedia used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007637 curator inference from encyclopedia A type of curator inference from encyclopedia that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007835 curator inference from Wikipedia used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007638 curator inference from Wikipedia A type of curator inference from Wikipedia that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007836 curator inference from Britannica used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007639 curator inference from Britannica A type of curator inference from Britannica that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007837 curator inference from MedlinePlus encyclopedia used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007640 curator inference from MedlinePlus encyclopedia A type of curator inference from MedlinePlus encyclopedia that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007838 curator inference from book used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007646 curator inference from book A type of curator inference from book that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007839 curator inference from dictionary used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007641 curator inference from dictionary A type of curator inference from dictionary that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007840 curator inference from MedlinePlus dictionary used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007644 curator inference from MedlinePlus dictionary A type of curator inference from MedlinePlus dictionary that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007841 curator inference from Merriam-Webster Dictionary used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007643 curator inference from Merriam-Webster Dictionary A type of curator inference from Merriam-Webster Dictionary that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007842 curator inference from Oxford Dictionary used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007642 curator inference from Oxford Dictionary A type of curator inference from Oxford Dictionary that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007843 curator inference from journal publication used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007645 curator inference from journal publication A type of curator inference from journal publication that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0001842 random mutagenesis of specific target DNA evidence http://purl.obolibrary.org/obo/ECO_0005529 random mutagenesis phenotypic evidence A type of random mutagenesis phenotypic evidence resulting from the random mutation of a specifically targeted region of DNA. http://purl.obolibrary.org/obo/ECO_0001843 random mutagenesis of specific target DNA evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007478 random mutagenesis evidence used in automatic assertion A type of random mutagenesis of specific target DNA evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0001844 random mutagenesis of specific target DNA evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005530 random mutagenesis evidence used in manual assertion A type of random mutagenesis of specific target DNA evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007850 inference of sequence features from visual inspection http://purl.obolibrary.org/obo/ECO_0000361 inferential evidence A type of inferential evidence in which sequence features are inferred based on visual inspection of the sequence. http://purl.obolibrary.org/obo/BFO_0000002 continuant http://purl.obolibrary.org/obo/BFO_0000001 entity An entity that exists in full at any time in which it exists at all, persists through time while maintaining its identity and has no temporal parts. http://purl.obolibrary.org/obo/BFO_0000003 occurrent http://purl.obolibrary.org/obo/BFO_0000001 entity An entity that has temporal parts and that happens, unfolds or develops through time. http://purl.obolibrary.org/obo/BFO_0000004 independent continuant http://purl.obolibrary.org/obo/BFO_0000002 continuant A continuant that is a bearer of quality and realizable entity entities, in which other entities inhere and which itself cannot inhere in anything. http://purl.obolibrary.org/obo/BFO_0000015 process http://purl.obolibrary.org/obo/BFO_0000003 occurrent An occurrent that has temporal proper parts and for some time t, p s-depends_on some material entity at t. http://purl.obolibrary.org/obo/BFO_0000017 realizable entity http://purl.obolibrary.org/obo/BFO_0000020 specifically dependent continuant A specifically dependent continuant that inheres in continuant entities and are not exhibited in full at every time in which it inheres in an entity or group of entities. The exhibition or actualization of a realizable entity is a particular manifestation, functioning or process that occurs under certain circumstances. http://purl.obolibrary.org/obo/BFO_0000020 specifically dependent continuant http://purl.obolibrary.org/obo/BFO_0000002 continuant A continuant that inheres in or is borne by other entities. Every instance of A requires some specific instance of B which must always be the same. http://purl.obolibrary.org/obo/BFO_0000023 role http://purl.obolibrary.org/obo/BFO_0000017 realizable entity A realizable entity the manifestation of which brings about some result or end that is not essential to a continuant in virtue of the kind of thing that it is but that can be served or participated in by that kind of continuant in some kinds of natural, social or institutional contexts. http://purl.obolibrary.org/obo/BFO_0000031 generically dependent continuant http://purl.obolibrary.org/obo/BFO_0000002 continuant A continuant that is dependent on one or other independent continuant bearers. For every instance of A requires some instance of (an independent continuant type) B but which instance of B serves can change from time to time. http://purl.obolibrary.org/obo/BFO_0000040 material entity http://purl.obolibrary.org/obo/BFO_0000004 independent continuant An independent continuant that is spatially extended whose identity is independent of that of other entities and can be maintained through time. http://purl.obolibrary.org/obo/IAO_0000003 measurement unit label http://purl.obolibrary.org/obo/IAO_0000009 datum label A measurement unit label is as a label that is part of a scalar measurement datum and denotes a unit of measure. http://purl.obolibrary.org/obo/IAO_0000005 objective specification http://purl.obolibrary.org/obo/IAO_0000033 directive information entity A directive information entity that describes an intended process endpoint. When part of a plan specification the concretization is realized in a planned process in which the bearer tries to effect the world so that the process endpoint is achieved. http://purl.obolibrary.org/obo/IAO_0000007 action specification http://purl.obolibrary.org/obo/IAO_0000033 directive information entity A directive information entity that describes an action the bearer will take. http://purl.obolibrary.org/obo/IAO_0000009 datum label http://purl.obolibrary.org/obo/IAO_0000030 information content entity A label is a symbol that is part of some other datum and is used to either partially define the denotation of that datum or to provide a means for identifying the datum as a member of the set of data with the same label http://purl.obolibrary.org/obo/IAO_0000015 information carrier http://purl.obolibrary.org/obo/BFO_0000019 quality A quality of an information bearer that imparts the information content http://purl.obolibrary.org/obo/IAO_0000027 data item http://purl.obolibrary.org/obo/IAO_0000030 information content entity An information content entity that is intended to be a truthful statement about something (modulo, e.g., measurement precision or other systematic errors) and is constructed/acquired by a method which reliably tends to produce (approximately) truthful statements. http://purl.obolibrary.org/obo/IAO_0000030 information content entity http://purl.obolibrary.org/obo/BFO_0000031 generically dependent continuant A generically dependent continuant that is about some thing. http://purl.obolibrary.org/obo/IAO_0000032 scalar measurement datum http://purl.obolibrary.org/obo/IAO_0000109 measurement datum A scalar measurement datum is a measurement datum that is composed of two parts, numerals and a unit label. http://purl.obolibrary.org/obo/IAO_0000033 directive information entity http://purl.obolibrary.org/obo/IAO_0000030 information content entity An information content entity whose concretizations indicate to their bearer how to realize them in a process. http://purl.obolibrary.org/obo/IAO_0000064 algorithm http://purl.obolibrary.org/obo/IAO_0000104 plan specification A plan specification which describes the inputs and output of mathematical functions as well as workflow of execution for achieving an predefined objective. Algorithms are realized usually by means of implementation as computer programs for execution by automata. http://purl.obolibrary.org/obo/IAO_0000078 curation status specification http://purl.obolibrary.org/obo/IAO_0000102 data about an ontology part The curation status of the term. The allowed values come from an enumerated list of predefined terms. See the specification of these instances for more detailed definitions of each enumerated value. http://purl.obolibrary.org/obo/IAO_0000101 image http://purl.obolibrary.org/obo/IAO_0000308 figure An image is an affine projection to a two dimensional surface, of measurements of some quality of an entity or entities repeated at regular intervals across a spatial range, where the measurements are represented as color and luminosity on the projected on surface. http://purl.obolibrary.org/obo/IAO_0000102 data about an ontology part http://purl.obolibrary.org/obo/IAO_0000027 data item Data about an ontology part is a data item about a part of an ontology, for example a term http://purl.obolibrary.org/obo/IAO_0000104 plan specification http://purl.obolibrary.org/obo/IAO_0000033 directive information entity A directive information entity with action specifications and objective specifications as parts that, when concretized, is realized in a process in which the bearer tries to achieve the objectives by taking the actions specified. http://purl.obolibrary.org/obo/IAO_0000109 measurement datum http://purl.obolibrary.org/obo/IAO_0000027 data item A measurement datum is an information content entity that is a recording of the output of a measurement such as produced by a device. http://purl.obolibrary.org/obo/IAO_0000178 material information bearer http://purl.obolibrary.org/obo/BFO_0000040 material entity A material entity in which a concretization of an information content entity inheres. http://purl.obolibrary.org/obo/IAO_0000225 obsolescence reason specification http://purl.obolibrary.org/obo/IAO_0000102 data about an ontology part The reason for which a term has been deprecated. The allowed values come from an enumerated list of predefined terms. See the specification of these instances for more detailed definitions of each enumerated value. http://purl.obolibrary.org/obo/IAO_0000308 figure http://purl.obolibrary.org/obo/IAO_0000030 information content entity An information content entity consisting of a two dimensional arrangement of information content entities such that the arrangement itself is about something. http://purl.obolibrary.org/obo/IAO_0000409 denotator type http://purl.obolibrary.org/obo/IAO_0000102 data about an ontology part A denotator type indicates how a term should be interpreted from an ontological perspective. http://purl.obolibrary.org/obo/IAO_0000414 mass measurement datum http://purl.obolibrary.org/obo/IAO_0000032 scalar measurement datum A scalar measurement datum that is the result of measurement of mass quality http://purl.obolibrary.org/obo/IDO_0000586 infection http://purl.obolibrary.org/obo/BFO_0000040 material entity A part of an extended organism that itself has as part a population of one or more infectious agents and that (1) exists as a result of processes initiated by members of the infectious agent population and is (2) clinically abnormal in virtue of the presence of this infectious agent population, or (3) has a disposition to bring clinical abnormality to immunocompetent organisms of the same Species as the host (the organism corresponding to the extended organism) through transmission of a member or offspring of a member of the infectious agent population. http://purl.obolibrary.org/obo/OBI_0000011 planned process http://purl.obolibrary.org/obo/BFO_0000015 process A process that realizes a plan which is the concretization of a plan specification. http://purl.obolibrary.org/obo/OBI_0000015 biological feature identification objective http://purl.obolibrary.org/obo/IAO_0000005 objective specification Biological_feature_identification_objective is an objective role carried out by the proposition defining the aim of a study designed to examine or characterize a particular biological feature. http://purl.obolibrary.org/obo/OBI_0000025 reference substance role http://purl.obolibrary.org/obo/BFO_0000023 role a role inhering in a material entity that is realized when characteristics or responses elicited by the substance are used for comparison or reference. http://purl.obolibrary.org/obo/OBI_0000038 chromatography column http://purl.obolibrary.org/obo/OBI_0000932 material separation device Chromatography column in chemistry is a tube and contents (typically glass) used to purify individual chemical compounds from mixtures of compounds. It is often used for preparative applications on scales from micrograms up to kilograms. http://purl.obolibrary.org/obo/OBI_0000043 xenotransplantation http://purl.obolibrary.org/obo/OBI_0000105 transplantation is the transplantation of living cells, tissues or \norgans from one species to another such as from pigs to humans http://purl.obolibrary.org/obo/OBI_0000047 processed material http://purl.obolibrary.org/obo/BFO_0000040 material entity Is a material entity that is created or changed during material processing. http://purl.obolibrary.org/obo/OBI_0000048 chromatography device http://purl.obolibrary.org/obo/OBI_0002089 physical store A device that facilitates the separation of mixtures. The function of a chromatography device involves passing a mixture dissolved in a "mobile phase" through a stationary phase, which separates the analyte to be measured from other molecules in the mixture and allows it to be isolated. http://purl.obolibrary.org/obo/OBI_0000049 mass spectrometer http://purl.obolibrary.org/obo/OBI_0000832 measurement device A mass spectrometer is an instrument which is used to measure the mass to charge ratio of ions. All mass spectrometers consist of three basic parts: an ion source, a mass analyzer, and a detector system. The stages within the mass spectrometer are: 1. Production of ions from the sample 2. Separation of ions with different masses 3. Detection of the number of ions of each mass produced 4.Collection of data to generate the mass spectrum http://purl.obolibrary.org/obo/OBI_0000057 allotransplantation http://purl.obolibrary.org/obo/OBI_0000105 transplantation is the transplantation of organs between members of the same species. http://purl.obolibrary.org/obo/OBI_0000064 positive reference substance role http://purl.obolibrary.org/obo/OBI_0000025 reference substance role a reference role in which the characteristics or responses elicited by the substance playing the reference substance role are used to establish a "100%" response http://purl.obolibrary.org/obo/OBI_0000067 evaluant role http://purl.obolibrary.org/obo/BFO_0000023 role a role that inheres in a material entity that is realized in an assay in which data is generated about the bearer of the evaluant role http://purl.obolibrary.org/obo/OBI_0000070 assay http://purl.obolibrary.org/obo/OBI_0000011 planned process A planned process with the objective to produce information about the material entity that is the evaluant, by physically examining it or its proxies. http://purl.obolibrary.org/obo/OBI_0000079 culture medium http://purl.obolibrary.org/obo/OBI_0000047 processed material a processed material that provides the needed nourishment for microorganisms or cells grown in vitro. http://purl.obolibrary.org/obo/OBI_0000086 reagent role http://purl.obolibrary.org/obo/BFO_0000023 role A role inhering in a biological or chemical entity that is intended to be applied in a scientific technique to participate (or have molecular components that participate) in a chemical reaction that facilitates the generation of data about some entity distinct from the bearer, or the generation of some specified material output distinct from the bearer. http://purl.obolibrary.org/obo/OBI_0000094 material processing http://purl.obolibrary.org/obo/OBI_0000011 planned process A planned process which results in physical changes in a specified input material http://purl.obolibrary.org/obo/OBI_0000097 participant under investigation role http://purl.obolibrary.org/obo/BFO_0000023 role A role that is realized through the execution of a study design in which the bearer of the role participates and in which data about that bearer is collected. http://purl.obolibrary.org/obo/OBI_0000105 transplantation http://purl.obolibrary.org/obo/OBI_0000094 material processing a protocol application to replace an organ or tissue of an organism http://purl.obolibrary.org/obo/OBI_0000112 specimen role http://purl.obolibrary.org/obo/BFO_0000023 role a role borne by a material entity that is gained during a specimen collection process and that can be realized by use of the specimen in an investigation http://purl.obolibrary.org/obo/OBI_0000113 sequence feature identification objective http://purl.obolibrary.org/obo/OBI_0000015 biological feature identification objective Sequence_feature_identification_objective is a biological_feature_identification_objective role describing a study designed to examine or characterize molecular features exhibited at the level of a macromolecular sequence, e.g. nucleic acid, protein, polysaccharide. http://purl.obolibrary.org/obo/OBI_0000115 intervention design http://purl.obolibrary.org/obo/OBI_0500000 study design An intervention design is a study design in which a controlled process applied to the subjects (the intervention) serves as the independent variable manipulated by the experimentalist. The treatment (perturbation or intervention) defined can be defined as a combination of values taken by independent variable manipulated by the experimentalists are applied to the recruited subjects assigned (possibly by applying specific methods) to treatment groups. The specificity of intervention design is the fact that independent variables are being manipulated and a response of the biological system is evaluated via response variables as monitored by possibly a series of assays. http://purl.obolibrary.org/obo/OBI_0000131 molecular feature identification objective http://purl.obolibrary.org/obo/OBI_0000015 biological feature identification objective Molecular_feature_identification_objective is a biological_feature_identification_objective role describing a study designed to examine or characterize molecular features of a biological system, e.g. expression profiling, copy number of molecular components, epigenetic modifications. http://purl.obolibrary.org/obo/OBI_0000151 filter paper http://purl.obolibrary.org/obo/OBI_0000932 material separation device A device manufacture with the intent to provide a porous unsized paper used for filtering. http://purl.obolibrary.org/obo/OBI_0000153 cell co-culturing http://purl.obolibrary.org/obo/OBI_0000094 material processing A material combination in which cell cultures of two or more different types are are combined and allowed to culture as one. http://purl.obolibrary.org/obo/OBI_0000164 clonal cDNA library http://purl.obolibrary.org/obo/OBI_0000731 recombinant vector Mixed population of cDNAs (complementaryDNA) made from mRNA from a defined source, usually a specific cell type. This term should be associated only to nucleic acid interactors not to their proteins product. For instance in 2h screening use living cells (MI:0349) as sample process. ALT DEF (PRS):: a cDNA library is a collection of host cells, typically E.Coli cells but not exclusively. modified by transfer of plasmid DNA molecule used as vector containing a fragment or totality of cDNA molecule (the insert) . cDNA library may have an array of role and applications. http://purl.obolibrary.org/obo/OBI_0000185 imaging assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that produces a picture of an entity. http://purl.obolibrary.org/obo/OBI_0000192 microtiter plate http://purl.obolibrary.org/obo/OBI_0000967 container A microtiter_plate is a flat plate with multiple wells used as small test tubes. http://purl.obolibrary.org/obo/OBI_00001975 chromatin immunoprecipitation http://purl.obolibrary.org/obo/OBI_0000690 immunoprecipitation An immunoprecipitation in which chromatin (i.e. packaged DNA which can include protein and RNA complexes) is cut into short regions, reversibly cross linked, and antibodies or tags are used to select for pieces of chromatin with desired characteristics. http://purl.obolibrary.org/obo/OBI_0000201 radioactivity detection http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that measures the amount of radiation in the radioactive spectrum (alpha, beta or gamma rays) emitted from an input material. http://purl.obolibrary.org/obo/OBI_0000219 cellular feature identification objective http://purl.obolibrary.org/obo/OBI_0000015 biological feature identification objective Cellular_feature_identification_objective is a biological_feature_identification_objective role describing a study designed to examine or characterize a biological feature monitored at the cellular level, e.g. stage of cell cycle, stage of differentiation. http://purl.obolibrary.org/obo/OBI_0000231 enzymatic cleavage http://purl.obolibrary.org/obo/OBI_0000094 material processing a material processing that digests the fraction of input material that is susceptible to that enzyme http://purl.obolibrary.org/obo/OBI_0000250 dye role http://purl.obolibrary.org/obo/OBI_0002743 molecular label role A molecular label role which inheres in a material entity and which is realized in the process of detecting a molecular dye that imparts color to some material of interest. http://purl.obolibrary.org/obo/OBI_0000253 artificially induced nucleic acid hybridization http://purl.obolibrary.org/obo/OBI_0000094 material processing Is a material transformation in which strands of nucleic acids that are (somewhat) complementary form a double-stranded molecule. Has input at least two single stranded molecules of nucleic acid molecules. http://purl.obolibrary.org/obo/OBI_0000257 DNA extraction http://purl.obolibrary.org/obo/OBI_0666667 nucleic acid extraction A DNA extraction is a nucleic acid extraction where the desired output material is DNA. http://purl.obolibrary.org/obo/OBI_0000268 organism feature identification objective http://purl.obolibrary.org/obo/OBI_0000015 biological feature identification objective Organism_feature_identification_objective is a biological_feature_identification_objective role describing a study designed to examine or characterize a biological feature monitored at the level of the organism, e.g. height, weight, stage of development, stage of life cycle. http://purl.obolibrary.org/obo/OBI_0000272 protocol http://purl.obolibrary.org/obo/IAO_0000104 plan specification A plan specification which has sufficient level of detail and quantitative information to communicate it between investigation agents, so that different investigation agents will reliably be able to independently reproduce the process. http://purl.obolibrary.org/obo/OBI_0000274 adding a material entity into a target http://purl.obolibrary.org/obo/OBI_0000652 material combination is a process with the objective to place a material entity bearing the 'material to be added role' into a material bearing the 'target of material addition role'. http://purl.obolibrary.org/obo/OBI_0000275 analyte role http://purl.obolibrary.org/obo/OBI_0002444 measurand role A measurand role borne by a molecular entity or an atom and realized in an analyte assay which achieves the objective to measure the magnitude/concentration/amount of the analyte in the entity bearing evaluant role. http://purl.obolibrary.org/obo/OBI_0000288 protein-protein interaction detection assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that determines interactions between proteins, such as protein-protein binding. http://purl.obolibrary.org/obo/OBI_0000318 eluate http://purl.obolibrary.org/obo/OBI_0000047 processed material a eluate is a material entity which results from an elution, e.g. from a chromatography column. it has as part a material entity with role mobile phase http://purl.obolibrary.org/obo/OBI_0000319 material to be added role http://purl.obolibrary.org/obo/BFO_0000023 role material to be added role is a protocol participant role realized by a material which is added into a material bearing the target of material addition role in a material addition process http://purl.obolibrary.org/obo/OBI_0000338 drawing a conclusion based on data http://purl.obolibrary.org/obo/OBI_0000011 planned process A planned process in which data gathered in an investigation is evaluated in the context of existing knowledge with the objective to generate more general conclusions or to conclude that the data does not allow one to draw general conclusion http://purl.obolibrary.org/obo/OBI_0000341 histological sample preparation http://purl.obolibrary.org/obo/OBI_0000659 specimen collection process histological sample preparation is the preparation of an input tissue via slicing and labeling to make tissue microstructure of interest visible in a future histology assay http://purl.obolibrary.org/obo/OBI_0000345 mass analyzer http://purl.obolibrary.org/obo/OBI_0000932 material separation device A Mass analyzer is a device that separates ions according to their mass-to-charge ratio. All mass spectrometers are based on dynamics of charged particles in electric and magnetic fields in vacuum where the two laws of Lorentz force law and Newton's second law of motion apply. http://purl.obolibrary.org/obo/OBI_0000361 ion source http://purl.obolibrary.org/obo/OBI_0000968 device An ion source is a device that is part of a mass spectrometer that ionizes the material under analysis. The ions are then transported by magnetic or electric fields to the mass analyzer. Techniques for ionization have been key to determining what types of samples can be analyzed by mass spectrometry. Electron ionization and chemical ionization are used for gases and vapors. In chemical ionization sources, the material is ionized by chemical ion-molecule reactions during collisions in the source. Two techniques often used with liquid and solid biological samples include electrospray ionization (due to John Fenn PMID 2675315.) and matrix-assisted laser desorption/ionization (MALDI, due to M. Karas and F. Hillenkamp (Measuring Mass: From Positive Rays to Proteins by Michael A. Grayson (Editor) (ISBN 0-941901-31-9))). http://purl.obolibrary.org/obo/OBI_0000364 ion detector http://purl.obolibrary.org/obo/OBI_0000832 measurement device An ion detector is a device that measures and records the charge induced or current produced when an ion passes by or hits a surface. Example: In a scanning instrument the signal produced in the detector during the course of the scan versus where the instrument is in the scan (at what m/Q) will produce a mass spectrum, a record of ions as a function of m/Q. http://purl.obolibrary.org/obo/OBI_0000366 metabolite profiling assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that detects and identifies chemical entities resulting from biochemical and cellular metabolism http://purl.obolibrary.org/obo/OBI_0000367 light emission function http://purl.obolibrary.org/obo/OBI_0000374 excitation function A light emission function is an excitation function to excite a material to a specific excitation state that it emits light. http://purl.obolibrary.org/obo/OBI_0000369 magnify function http://purl.obolibrary.org/obo/BFO_0000034 function A magnify function is a function to increase the size of a transmitted object image through the precise arrangement of energy diffraction elements along an imaging path. http://purl.obolibrary.org/obo/OBI_0000370 contain function http://purl.obolibrary.org/obo/BFO_0000034 function A contain function is a function to constrain a material entities location in space http://purl.obolibrary.org/obo/OBI_0000371 heat function http://purl.obolibrary.org/obo/BFO_0000034 function A heat function is a function that increases the internal kinetic energy of a material http://purl.obolibrary.org/obo/OBI_0000372 material separation function http://purl.obolibrary.org/obo/BFO_0000034 function A material separation function is a function that increases the resolution between two or more material entities. The to distinction between the entities is usually based on some associated physical quality. http://purl.obolibrary.org/obo/OBI_0000374 excitation function http://purl.obolibrary.org/obo/BFO_0000034 function A excitation function is a function to inject energy by bombarding a material with energetic particles (e.g., photons) thereby imbuing internal material components such as electrons with additional energy. These internal, 'excited' particles may lead to the rupturing of covalent chemical bonds or may quickly relax back to there unexcited state with an exponential time course thereby locally emitting energy in the form of photons. http://purl.obolibrary.org/obo/OBI_0000376 synthesizing function http://purl.obolibrary.org/obo/BFO_0000034 function A synthesizing function is a function to assemble new output materials from distinct input materials. The output materials typically consist of chemically distinct monomeric objects or object aggregate polymers. http://purl.obolibrary.org/obo/OBI_0000377 perturb function http://purl.obolibrary.org/obo/BFO_0000034 function A perturb function is a function that disrupts the normal function of a system induced through either internal or external means. External means of perturbation include: (1) displacement fields in the physical sense - e.g., temperature change, osmotic shock, pressure change; (2) application of small molecules such as drugs or toxins to perturb the function of specific pathways or application of surfactants to perturb the normal function of plasma membrane. Internal means of perturbation include: (1) manipulation of gene function via gene knockout or transcript knockdown via RNAi; (2) directed genetic mutation leading to minimal aa alterations that interfere with peptide function. http://purl.obolibrary.org/obo/OBI_0000378 filter function http://purl.obolibrary.org/obo/OBI_0000372 material separation function A filter function is a function to prevent the flow of certain entities based on a quality or qualities of the entity while allowing entities which have different qualities to pass through http://purl.obolibrary.org/obo/OBI_0000379 mechanical function http://purl.obolibrary.org/obo/BFO_0000034 function A mechanical function is a function that is realised via mechanical work (through an certain amount of energy transferred by some force). http://purl.obolibrary.org/obo/OBI_0000384 electricity supply function http://purl.obolibrary.org/obo/OBI_0000391 energy supply function An electricity supply function is an energy supply function to transfer electricity from one source to another, typically a consumer of the electricity or as a stimulus during a neuroscience experiment. http://purl.obolibrary.org/obo/OBI_0000385 ionization function http://purl.obolibrary.org/obo/BFO_0000034 function An ionization function is a function to physically convert an atom or molecule into an ion by adding or removing charged particles such as electrons or other ions. http://purl.obolibrary.org/obo/OBI_0000387 cool function http://purl.obolibrary.org/obo/BFO_0000034 function A cool function is a function to decrease the internal kinetic energy of a material below the initial kinetic energy of that type of material. http://purl.obolibrary.org/obo/OBI_0000391 energy supply function http://purl.obolibrary.org/obo/BFO_0000034 function An energy supply function is a function to supply or transfer energy from an energy source to a consumer of the energy http://purl.obolibrary.org/obo/OBI_0000397 image acquisition function http://purl.obolibrary.org/obo/OBI_0000453 measure function An image acquisition function is a function to acquire an image of a material http://purl.obolibrary.org/obo/OBI_0000398 image creation device http://purl.obolibrary.org/obo/OBI_0000832 measurement device An image creation device is a device which captures a digitized image of an object http://purl.obolibrary.org/obo/OBI_0000399 solid support function http://purl.obolibrary.org/obo/BFO_0000034 function A solid support function is a function of a device on which an entity is kept in a defined position and prevented in its movement http://purl.obolibrary.org/obo/OBI_0000401 environment control function http://purl.obolibrary.org/obo/BFO_0000034 function An environmental control function is a function that regulates a contained environment within specified parameter ranges. For example the control of light exposure, humidity and temperature. http://purl.obolibrary.org/obo/OBI_0000403 sort function http://purl.obolibrary.org/obo/OBI_0000372 material separation function A sort function is a function to distinguish material components based on some associated physical quality or entity and to partition the separate components into distinct fractions according to a defined order. http://purl.obolibrary.org/obo/OBI_0000406 PCR product http://purl.obolibrary.org/obo/CHEBI_4705 double-stranded DNA is double stranded DNA that is the specified output of a polymerase chain reaction http://purl.obolibrary.org/obo/OBI_0000409 nucleic acid template role http://purl.obolibrary.org/obo/OBI_0000025 reference substance role a reference substance role which inheres in nucleic acid material entity and is realized in the process of using the nucleic acid bearing the template role as a reference during synthesis of a reverse copy. http://purl.obolibrary.org/obo/OBI_0000411 cloning vector role http://purl.obolibrary.org/obo/OBI_0000319 material to be added role A material to be added role played by a small, self-replicating DNA or RNA molecule - usually a plasmid or chromosome - and realized in a process whereby foreign DNA or RNA is inserted into the vector during the process of cloning. http://purl.obolibrary.org/obo/OBI_0000415 polymerase chain reaction http://purl.obolibrary.org/obo/OBI_0600058 enzymatic amplification PCR is the process in which a DNA polymerase is used to amplify a piece of DNA by in vitro enzymatic replication. As PCR progresses, the DNA thus generated is itself used as a template for replication. This sets in motion a chain reaction in which the DNA template is exponentially amplified. http://purl.obolibrary.org/obo/OBI_0000416 cloning insert role http://purl.obolibrary.org/obo/BFO_0000023 role cloning insert role is a role which inheres in DNA or RNA and is realized by the process of being inserted into a cloning vector in a cloning process. http://purl.obolibrary.org/obo/OBI_0000419 reverse transcriptase http://purl.obolibrary.org/obo/OBI_0000427 enzyme enzyme and has_function some GO:0003964 (RNA-directed DNA polymerase activity) http://purl.obolibrary.org/obo/OBI_0000423 extract http://purl.obolibrary.org/obo/OBI_0000047 processed material an extract is a material entity which results from an extraction process http://purl.obolibrary.org/obo/OBI_0000424 transcription profiling assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that determines gene expression and transcription activity using ribonucleic acids collected from a material entity. http://purl.obolibrary.org/obo/OBI_0000427 enzyme http://purl.obolibrary.org/obo/BFO_0000040 material entity (protein or rna) or has_part (protein or rna) and has_function some GO:0003824 (catalytic activity) http://purl.obolibrary.org/obo/OBI_0000432 polyacrylamide gel http://purl.obolibrary.org/obo/OBI_0000047 processed material a material entity resulting from the polymerization of acrylamide with TEMED in some buffer solution http://purl.obolibrary.org/obo/OBI_0000434 adding material objective http://purl.obolibrary.org/obo/OBI_0000686 material combination objective is the specification of an objective to add a material into a target material. The adding is asymmetric in the sense that the target material largely retains its identity http://purl.obolibrary.org/obo/OBI_0000435 genotyping assay http://purl.obolibrary.org/obo/OBI_0000443 analyte assay An assay which generates data about a genotype from a specimen of genomic DNA. A variety of techniques and instruments can be used to produce information about sequence variation at particular genomic positions. http://purl.obolibrary.org/obo/OBI_0000437 analyte measurement objective http://purl.obolibrary.org/obo/OBI_0000441 assay objective an assay objective to determine the presence or concentration of an analyte in the evaluant http://purl.obolibrary.org/obo/OBI_0000439 agarose gel http://purl.obolibrary.org/obo/OBI_0000047 processed material a material entity resulting from the polymerization of agarose after heating agarose suspended in some buffer solution http://purl.obolibrary.org/obo/OBI_0000441 assay objective http://purl.obolibrary.org/obo/IAO_0000005 objective specification an objective specification to determine a specified type of information about an evaluated entity (the material entity bearing evaluant role) http://purl.obolibrary.org/obo/OBI_0000443 analyte assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay with the objective to capture information about the presence, concentration, or amount of an analyte in an evaluant. http://purl.obolibrary.org/obo/OBI_0000444 target of material addition role http://purl.obolibrary.org/obo/BFO_0000023 role target of material addition role is a role realized by an entity into which a material is added in a material addition process http://purl.obolibrary.org/obo/OBI_0000447 intra cellular electrophysiology recording assay http://purl.obolibrary.org/obo/OBI_0000070 assay An electrophysiology recording assay where the recording location of the electrode is intracellular http://purl.obolibrary.org/obo/OBI_0000453 measure function http://purl.obolibrary.org/obo/BFO_0000034 function Measure function is a function that is borne by a processed material and realized in a process in which information about some entity is expressed relative to some reference. http://purl.obolibrary.org/obo/OBI_0000454 extracellular electrophysiology recording assay http://purl.obolibrary.org/obo/OBI_0000070 assay An electrophysiology recording assay where the recording location of the electrode is extracellular http://purl.obolibrary.org/obo/OBI_0000456 material transformation objective http://purl.obolibrary.org/obo/IAO_0000005 objective specification an objective specifiction that creates an specific output object from input materials. http://purl.obolibrary.org/obo/OBI_0000470 mass spectrometry assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that identifies the amount and type of material entities present in a sample by fragmenting the sample and measuring the mass-to-charge ratio of the resulting particles. http://purl.obolibrary.org/obo/OBI_0000471 study design execution http://purl.obolibrary.org/obo/OBI_0000011 planned process a planned process that carries out a study design http://purl.obolibrary.org/obo/OBI_0000533 affinity column http://purl.obolibrary.org/obo/OBI_0000038 chromatography column An affinity column is a chromatography column that is used in affinity chromatography. Differences in the affinity of molecules to be separated to a stationary phase are used for discriminate retention. http://purl.obolibrary.org/obo/OBI_0000535 gel filtration column http://purl.obolibrary.org/obo/OBI_0000618 size exclusion column A Gel filtration column is a chromatography column for size-exclusion chromatography, in which the stationary phase is a gel. The main application of gel filtration chromatography is the fractionation of proteins and other water-soluble polymers. http://purl.obolibrary.org/obo/OBI_0000552 reverse transcribed polymerase chain reaction http://purl.obolibrary.org/obo/OBI_0600058 enzymatic amplification reverse transcribe pcr is a process which allow amplification of cDNA during a pcr reaction while the cDNA results from a retrotranscription of messenger RNA isolated from a material entity. http://purl.obolibrary.org/obo/OBI_0000576 scattered molecular aggregate http://purl.obolibrary.org/obo/BFO_0000040 material entity a material entity that consists of all the molecules of a specific type that are located in some bounded region and which is part of a more massive material entity that has parts that are other such aggregates http://purl.obolibrary.org/obo/OBI_0000601 chromatography consumable http://purl.obolibrary.org/obo/OBI_0000048 chromatography device A chromatography consumable is a consumable that is used in a chromatography experiment. http://purl.obolibrary.org/obo/OBI_0000618 size exclusion column http://purl.obolibrary.org/obo/OBI_0000038 chromatography column A size exclusion column is a chromatography column as used in size exclusion chromatography and which enables the separation of mixtures according to differrences in molecular size. http://purl.obolibrary.org/obo/OBI_0000623 NMR spectroscopy assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that exploits the magnetic properties of certain nuclei (those with a spin) to resonate when placed in particular magnetic field conditions. Instruments recording NMR spectrum and sets of analysis can be used to deduce identity of chemical as well as composition of complex chemical mixtures. http://purl.obolibrary.org/obo/OBI_0000626 DNA sequencing assay http://purl.obolibrary.org/obo/OBI_0600047 sequencing assay A sequencing assay which determines information on the sequence of a DNA molecule. http://purl.obolibrary.org/obo/OBI_0000630 hematology assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that studies blood and blood producing organs using a variety of techniques and instruments http://purl.obolibrary.org/obo/OBI_0000634 DNA methylation profiling assay http://purl.obolibrary.org/obo/OBI_0002020 epigenetic modification assay An assay that measures the state of methylation of DNA molecules using genomic DNA collected from a material entity using a range of techniques and instrument such as DNA sequencers and often relying on treatment with bisulfites to ensure cytosine conversion. http://purl.obolibrary.org/obo/OBI_0000639 material separation objective http://purl.obolibrary.org/obo/OBI_0000456 material transformation objective is an objective to transform a material entity into spatially separated components. http://purl.obolibrary.org/obo/OBI_0000650 differential expression analysis data transformation http://purl.obolibrary.org/obo/OBI_0200000 data transformation A differential expression analysis data transformation is a data transformation that has an objective of differential expression analysis. Frequently this data transformation involves summarizing or otherwise aggregating signals from various samples categorized into groups, and calculating measures of group differences from these aggregated signals. http://purl.obolibrary.org/obo/OBI_0000651 urine specimen http://purl.obolibrary.org/obo/OBI_0001479 specimen from organism a portion of urine collected from an organism http://purl.obolibrary.org/obo/OBI_0000652 material combination http://purl.obolibrary.org/obo/OBI_0000094 material processing is a material processing with the objective to combine two or more material entities as input into a single material entity as output. http://purl.obolibrary.org/obo/OBI_0000659 specimen collection process http://purl.obolibrary.org/obo/OBI_0000011 planned process A planned process with the objective of collecting a specimen. http://purl.obolibrary.org/obo/OBI_0000664 BrdU incorporation assay http://purl.obolibrary.org/obo/OBI_0000891 cell proliferation assay A cell proliferation assay in which cells are cultured in the presence of BrdU which is incorporated into newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication, which can be quantified by BrdU specific antibodies. http://purl.obolibrary.org/obo/OBI_0000669 tritiated thymidine incorporation assay http://purl.obolibrary.org/obo/OBI_0000891 cell proliferation assay A cell proliferation assay in which cells are cultured in the presence of tritiated thymidine which is incorporated into newly synthesized DNA of replicating cells (during the S phase of the cell cycle). The radioactivity of tritiated thymidine in a cell is a proxy for cells that were actively replicating. http://purl.obolibrary.org/obo/OBI_0000671 sample from organism http://purl.obolibrary.org/obo/OBI_0001479 specimen from organism a material obtained from an organism in order to be a representative of the whole http://purl.obolibrary.org/obo/OBI_0000678 portioning objective http://purl.obolibrary.org/obo/OBI_0000639 material separation objective A material separation objective aiming to separate material into multiple portions, each of which contains a similar composition of the input material. http://purl.obolibrary.org/obo/OBI_0000681 separation into different composition objective http://purl.obolibrary.org/obo/OBI_0000639 material separation objective A material separation objective aiming to separate a material entity that has parts of different types, and end with at least one output that is a material with parts of fewer types (modulo impurities). http://purl.obolibrary.org/obo/OBI_0000684 specimen collection objective http://purl.obolibrary.org/obo/IAO_0000005 objective specification A objective specification to obtain a material entity for potential use as an input during an investigation. http://purl.obolibrary.org/obo/OBI_0000685 creating a mixture of molecules in solution http://purl.obolibrary.org/obo/OBI_0000652 material combination is a process with the objective to prepare a liquid solution of one or more chemicals at desired concentrations. http://purl.obolibrary.org/obo/OBI_0000686 material combination objective http://purl.obolibrary.org/obo/OBI_0000456 material transformation objective is an objective to obtain an output material that contains several input materials. http://purl.obolibrary.org/obo/OBI_0000690 immunoprecipitation http://purl.obolibrary.org/obo/OBI_0600014 material component separation is a process which realizes a material separation objective by relying on antibodies to specifically binding to material entity http://purl.obolibrary.org/obo/OBI_0000699 survival assessment assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that measures the occurrence of death events in one or more organisms over time http://purl.obolibrary.org/obo/OBI_0000711 library preparation http://purl.obolibrary.org/obo/OBI_0000094 material processing is a process which results in the creation of a library from fragments of DNA using cloning vectors or oligonucleotides with the role of adaptors. http://purl.obolibrary.org/obo/OBI_0000716 ChIP-seq assay http://purl.obolibrary.org/obo/OBI_0001954 ChIP assay An assay in which chromatin is immunoprecipitated and subsequently analyzed using a DNA sequencing step to identify which parts of DNA are part of the isolated chromatin http://purl.obolibrary.org/obo/OBI_0000722 paired-end library http://purl.obolibrary.org/obo/OBI_0000047 processed material is a collection of short paired tags from the two ends of DNA fragments are extracted and covalently linked as ditag constructs http://purl.obolibrary.org/obo/OBI_0000731 recombinant vector http://purl.obolibrary.org/obo/OBI_0000047 processed material A recombinant vector is created by a recombinant vector cloning process, and contains nucleic acids that can be amplified. It retains functions of the original cloning vector. http://purl.obolibrary.org/obo/OBI_0000736 single fragment library http://purl.obolibrary.org/obo/OBI_0000047 processed material is a collection of short tags from DNA fragments, are extracted and covalently linked as single tag constructs http://purl.obolibrary.org/obo/OBI_0000737 cloning vector http://purl.obolibrary.org/obo/OBI_1110108 material to be added A cloning vector is an engineered material that is used as an input material for a recombinant vector cloning process to carry inserted nucleic acids. It contains an origin of replication for a specific destination host organism, encodes for a selectable gene product and contains a cloning site. http://purl.obolibrary.org/obo/OBI_0000740 material sample role http://purl.obolibrary.org/obo/OBI_0000112 specimen role A material sample role is a specimen role borne by a material entity that is the output of a material sampling process. http://purl.obolibrary.org/obo/OBI_0000744 material sampling process http://purl.obolibrary.org/obo/OBI_0000659 specimen collection process A specimen gathering process with the objective to obtain a specimen that is representative of the input material entity http://purl.obolibrary.org/obo/OBI_0000747 material sample http://purl.obolibrary.org/obo/OBI_0100051 specimen A material entity that has the material sample role http://purl.obolibrary.org/obo/OBI_0000750 study design independent variable http://purl.obolibrary.org/obo/IAO_0000033 directive information entity a directive information entity that is part of a study design. Independent variables are entities whose values are selected to determine its relationship to an observed phenomenon (the dependent variable). In such an experiment, an attempt is made to find evidence that the values of the independent variable determine the values of the dependent variable (that which is being measured). The independent variable can be changed as required, and its values do not represent a problem requiring explanation in an analysis, but are taken simply as given. The dependent variable on the other hand, usually cannot be directly controlled http://purl.obolibrary.org/obo/OBI_0000789 survival rate http://purl.obolibrary.org/obo/IAO_0000109 measurement datum A measurement data that represents the percentage of people or animals in a study or treatment group who are alive for a given period of time after diagnosis or initiation of monitoring. http://purl.obolibrary.org/obo/OBI_0000796 purification objective http://purl.obolibrary.org/obo/OBI_0000681 separation into different composition objective The objective to separate a material entity into different compositions of which one or more have are purified fractions that contain higher concentration of a desired component, while others contain impurities and are not of interest http://purl.obolibrary.org/obo/OBI_0000800 cross linking http://purl.obolibrary.org/obo/OBI_0000094 material processing A process in which bonds are created that link one polymer to another http://purl.obolibrary.org/obo/OBI_0000806 material maintenance objective http://purl.obolibrary.org/obo/IAO_0000005 objective specification An objective specification maintains some or all of the qualities of a material over time. http://purl.obolibrary.org/obo/OBI_0000807 presentation of stimulus http://purl.obolibrary.org/obo/OBI_0000011 planned process a planned process in which an organism is exposed to some stimulus with the intent to invoke an action http://purl.obolibrary.org/obo/OBI_0000809 amplified DNA http://purl.obolibrary.org/obo/CHEBI_16991 deoxyribonucleic acid DNA that has been produced in an enzymatic amplification process http://purl.obolibrary.org/obo/OBI_0000811 primary structure of DNA macromolecule http://purl.obolibrary.org/obo/SO_0000001 region a quality of a DNA molecule that inheres in its bearer due to the order of its DNA nucleotide residues. http://purl.obolibrary.org/obo/OBI_0000816 micro electrode http://purl.obolibrary.org/obo/OBI_0000832 measurement device An electrode of very fine caliber consisting usually of a fine wire or a glass tube of capillary diameter drawn to a fine point and filled with saline or a metal used in physiological experiments to stimulate or record action currents of extracellular or intracellular origin in the nervous system. http://purl.obolibrary.org/obo/OBI_0000832 measurement device http://purl.obolibrary.org/obo/OBI_0000968 device A device in which a measure function inheres. http://purl.obolibrary.org/obo/OBI_0000834 high molecular weight DNA extract http://purl.obolibrary.org/obo/OBI_0001051 DNA extract The output of an extraction process in which DNA molecules above a molecular weight cutoff are purified in order to exclude DNA from organellas. http://purl.obolibrary.org/obo/OBI_0000838 material maintenance http://purl.obolibrary.org/obo/OBI_0000011 planned process a process with that achieves the objective to maintain some or all of the characteristics of an input material over time http://purl.obolibrary.org/obo/OBI_0000845 primary structure of RNA molecule http://purl.obolibrary.org/obo/SO_0000001 region The primary structure of an RNA molecule that is completely defined by the set of its nucleic residue parts and the linear order induced by the peptide bonds that hold them together http://purl.obolibrary.org/obo/OBI_0000854 western blot assay http://purl.obolibrary.org/obo/OBI_0001978 immunoblot assay An analyte assay that detects specific peptides in an input material by separating it using gel electrophoresis, transfering the separated molecules to a membrane, and staining them with_ antibodies specific to the analyte molecules. http://purl.obolibrary.org/obo/OBI_0000857 complementary nucleotide probe role http://purl.obolibrary.org/obo/BFO_0000023 role A role played by a nucleic acid molecule that is used in a planned process for its ability to bind a nucleic acid molecules with complementary nucleotide sequence http://purl.obolibrary.org/obo/OBI_0000860 northern blot assay http://purl.obolibrary.org/obo/OBI_0000443 analyte assay An assay that determines the presence of gene transcripts by hybridizing labeled RNA or DNA probes against messenger RNAs isolated from tissue or cell cultures, resolved on denaturing gel, transfered by a blotting procedure to a solid support. Detection of hybridization signals is carried out by immunofluorescence or radioactivity measurements using photographic films or digital imaging devices such as Phosphor Imager. http://purl.obolibrary.org/obo/OBI_0000873 pre-mortem specimen http://purl.obolibrary.org/obo/OBI_0001506 specimen with pre- or post-mortem status a specimen that was taken from a live organism http://purl.obolibrary.org/obo/OBI_0000874 detection of specific nucleic acid polymers with complementary probes http://purl.obolibrary.org/obo/OBI_0000443 analyte assay An analyte assay in which a specified input material (the evaluant) is examined for the presence or quantity of specified nucleic acid polymers, which are identified based on the use of complementary nucleic acid probes. http://purl.obolibrary.org/obo/OBI_0000880 RNA extract http://purl.obolibrary.org/obo/OBI_0001010 nucleic acid extract an extract which is the output of an extraction process in which RNA molecules are isolated from a specimen. http://purl.obolibrary.org/obo/OBI_0000882 cell-cell killing assay http://purl.obolibrary.org/obo/OBI_0001977 cytometry assay A cytometry assay that monitors a cell population to track how many are killed by other cells. http://purl.obolibrary.org/obo/OBI_0000883 in vivo cell killing assay http://purl.obolibrary.org/obo/OBI_0000882 cell-cell killing assay A cell killing assay that measures if and how many target cells are killed within an organism. http://purl.obolibrary.org/obo/OBI_0000891 cell proliferation assay http://purl.obolibrary.org/obo/OBI_0001977 cytometry assay A cytometry assay which measures the degreee to which input cells are replicating. http://purl.obolibrary.org/obo/OBI_0000892 Southern blot assay http://purl.obolibrary.org/obo/OBI_0000443 analyte assay An analyte assay that detects the presence of a specific sequence in a DNA sample, which has been digested by restriction enzymes, resolved by gel electrophoresis, and blotted to a solid support, followed by hybridization of a probe raised against a specific sequence and detected with fluorescence or radioactivity. http://purl.obolibrary.org/obo/OBI_0000893 real time polymerase chain reaction assay http://purl.obolibrary.org/obo/OBI_0002692 polymerase chain reaction assay An assay, based on the PCR, that amplifies and simultaneously quantifies a specific DNA molecule based on the use of complementary probes/primers. It enables both detection and quantification (as absolute number of copies or relative amount when normalized to DNA input or additional normalizing genes) of one or more specific sequences in a DNA sample. http://purl.obolibrary.org/obo/OBI_0000902 post mortem specimen http://purl.obolibrary.org/obo/OBI_0001506 specimen with pre- or post-mortem status a specimen that was taken from a dead organism http://purl.obolibrary.org/obo/OBI_0000903 in vitro cell killing assay http://purl.obolibrary.org/obo/OBI_0000882 cell-cell killing assay A cell killing assay that measures if and how many target cells are actively killed by other cells in a cell culture. http://purl.obolibrary.org/obo/OBI_0000912 X-ray crystallography 3D molecular structure determination assay http://purl.obolibrary.org/obo/OBI_0003117 3D molecular structure determination assay A 3D molecular structure determination assay in which the diffraction of pattern of X-ray beams in a crystal of purified material entities is used to resolve the 3-dimensional structure of the material entity of interest. http://purl.obolibrary.org/obo/OBI_0000913 promoter activity detection by reporter gene assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay in which the activity of a promoter in a cell is monitored by using a reporter gene that was inserted in a genomic location under control of the promoter and whose expression can be easily detected based on qualities or functions of the gene. http://purl.obolibrary.org/obo/OBI_0000916 flow cytometry assay http://purl.obolibrary.org/obo/OBI_0001977 cytometry assay A cytometry assay in which an input cell population is put in solution, is passed by a laser, and optical sensors are used to detect scattering of the laser light and/or fluorescence of specific markers to count and characterize the particles in solution. http://purl.obolibrary.org/obo/OBI_0000921 labeled RNA extract http://purl.obolibrary.org/obo/OBI_0001143 labeled nucleic acid extract a labeled specimen that is the output of a labeling process and has grain labeled RNA to be able to detect RNA in future experiments. http://purl.obolibrary.org/obo/OBI_0000923 surface plasmon resonance binding assay http://purl.obolibrary.org/obo/OBI_0001146 binding assay A binding assay that uses the detection of electromagnetic waves in a surface to detect material entities adsorbed to the surface, which change the local optical index of refraction. http://purl.obolibrary.org/obo/OBI_0000924 labeled specimen http://purl.obolibrary.org/obo/OBI_0001936 molecular-labeled material A specimen that has been modified in order to be able to detect it in future experiments http://purl.obolibrary.org/obo/OBI_0000925 infectious agent http://purl.obolibrary.org/obo/OBI_0100026 organism is a material entity bearing the disposition to infect an organism http://purl.obolibrary.org/obo/OBI_0000930 calorimeter http://purl.obolibrary.org/obo/OBI_0000832 measurement device A measurement device that is used to calculate the heat flow of a chemical reaction or physical change. http://purl.obolibrary.org/obo/OBI_0000931 study intervention http://purl.obolibrary.org/obo/OBI_0000011 planned process the part of the execution of an intervention design study which is varied between two or more subjects in the study http://purl.obolibrary.org/obo/OBI_0000932 material separation device http://purl.obolibrary.org/obo/OBI_0000968 device A device with a separation function realized in a planed process http://purl.obolibrary.org/obo/OBI_0000953 processed specimen http://purl.obolibrary.org/obo/OBI_0100051 specimen A specimen that has been intentionally physically modified. http://purl.obolibrary.org/obo/OBI_0000964 in live cell assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay in which a measurement is made by observing entities located in a live cell. http://purl.obolibrary.org/obo/OBI_0000966 in live organism assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay in which a measurement is made by observing entities located in an organism. http://purl.obolibrary.org/obo/OBI_0000967 container http://purl.obolibrary.org/obo/OBI_0000968 device A device that can be used to restrict the location of material entities over time http://purl.obolibrary.org/obo/OBI_0000968 device http://purl.obolibrary.org/obo/OBI_0000047 processed material A material entity that is designed to perform a function in a scientific investigation, but is not a reagent. http://purl.obolibrary.org/obo/OBI_0000975 cell-cell binding detection by flow cytometry assay http://purl.obolibrary.org/obo/OBI_0001977 cytometry assay A binding assay which uses a flow cytometer to detect pairs of cells that are bound to each other by staining them with different fluorescent labels. http://purl.obolibrary.org/obo/OBI_0000978 in container assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay in which a measurement is made by observing entities located in a container. http://purl.obolibrary.org/obo/OBI_0000985 growth condition intervention design http://purl.obolibrary.org/obo/OBI_0000115 intervention design A study design in which the independent variable is the environmental condition in which the specimen is growing http://purl.obolibrary.org/obo/OBI_0000989 PCR instrument http://purl.obolibrary.org/obo/OBI_0400116 thermal cycler A device that is used to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating thousands to millions of copies of a particular DNA sequence. http://purl.obolibrary.org/obo/OBI_0000990 electron microscope http://purl.obolibrary.org/obo/OBI_0400169 microscope A microscope that produces an image of an object by targeting it with an electron beam http://purl.obolibrary.org/obo/OBI_0000997 growth environment http://purl.obolibrary.org/obo/BFO_0000040 material entity The collection of material entities and their qualities that are located near a live organism, tissue or cell and can influence its growth. http://purl.obolibrary.org/obo/OBI_0001007 image creation http://purl.obolibrary.org/obo/OBI_0000011 planned process A planned process that captures an image of an object. http://purl.obolibrary.org/obo/OBI_0001010 nucleic acid extract http://purl.obolibrary.org/obo/OBI_0000953 processed specimen An extract that is the output of an extraction process in which nucleic acid molecules are isolated from a specimen. http://purl.obolibrary.org/obo/OBI_0001025 binding constant determination assay http://purl.obolibrary.org/obo/OBI_0001146 binding assay A binding assay where the specified output is a binding constant http://purl.obolibrary.org/obo/OBI_0001032 light emission device http://purl.obolibrary.org/obo/OBI_0000968 device A device which has a function to emit light. http://purl.obolibrary.org/obo/OBI_0001033 perturbation device http://purl.obolibrary.org/obo/OBI_0000968 device A perturbation device is a device which is designed to perform a perturb function http://purl.obolibrary.org/obo/OBI_0001034 environmental control device http://purl.obolibrary.org/obo/OBI_0000968 device An environmental control device is a device which has the function to control some aspect of the environment such as temperature, or humidity. http://purl.obolibrary.org/obo/OBI_0001051 DNA extract http://purl.obolibrary.org/obo/OBI_0001010 nucleic acid extract The output of an extraction process in which DNA molecules are purified in order to exclude DNA from organellas. http://purl.obolibrary.org/obo/OBI_0001053 electrophoresis system http://purl.obolibrary.org/obo/OBI_0000932 material separation device A device that moves charged particles through a medium by using an electric field induced by electrodes. http://purl.obolibrary.org/obo/OBI_0001057 high performance liquid chromatography instrument http://purl.obolibrary.org/obo/OBI_0001139 liquid chromatography instrument A liquid chromatography instrument that consists of a reservoir of mobile phase, a pump, an injector, a separation column, and a detector. The pump (rather than gravity) provides the higher pressure required to propel the mobile phase and analyte through the densely packed column. http://purl.obolibrary.org/obo/OBI_0001079 confocal microscope http://purl.obolibrary.org/obo/OBI_0400169 microscope A microscope that is used to increase micrograph contrast and/or reconstruct three-dimensional images by using a spatial pinhole to eliminate out-of-focus light in specimens that are thicker than the focal plane. http://purl.obolibrary.org/obo/OBI_0001080 patch clamp device http://purl.obolibrary.org/obo/OBI_0000832 measurement device A device used in electrophysiology that allows the study of single or multiple ion channels in cells. http://purl.obolibrary.org/obo/OBI_0001108 nucleic acid sequencer http://purl.obolibrary.org/obo/OBI_0000832 measurement device An device that is used to determine the order of nucleotides in nucleic acid sequences. http://purl.obolibrary.org/obo/OBI_0001121 gel electrophoresis system http://purl.obolibrary.org/obo/OBI_0001053 electrophoresis system An electrophoresis system in which an electric field is applied to a gel matrix http://purl.obolibrary.org/obo/OBI_0001129 voltage clamp device http://purl.obolibrary.org/obo/OBI_0000832 measurement device A device that is used to measure the ion currents across the membrane of excitable cells, such as neurons, while holding the membrane voltage at a set level. http://purl.obolibrary.org/obo/OBI_0001136 surface plasmon resonance instrument http://purl.obolibrary.org/obo/OBI_0000832 measurement device A tool for measuring adsorption of material onto planar metal (typically gold and silver) surfaces or onto the surface of metal nanoparticles. http://purl.obolibrary.org/obo/OBI_0001137 protein sequencer http://purl.obolibrary.org/obo/OBI_0000832 measurement device An device that is used to determine the order of amino acids in protein sequences. http://purl.obolibrary.org/obo/OBI_0001138 X-ray source http://purl.obolibrary.org/obo/OBI_0000968 device A device that is used to generate X-rays. http://purl.obolibrary.org/obo/OBI_0001139 liquid chromatography instrument http://purl.obolibrary.org/obo/OBI_0000048 chromatography device A chromatography device that dissolves a mixture in liquid mobile phase to separate the analyte to be measured from other molecules in the mixture and allows it to be isolated http://purl.obolibrary.org/obo/OBI_0001143 labeled nucleic acid extract http://purl.obolibrary.org/obo/OBI_0000924 labeled specimen a labeled specimen that is the output of a labeling process and has grain labeled nucleic acid for detection of the nucleic acid in future experiments. http://purl.obolibrary.org/obo/OBI_0001144 binding constant http://purl.obolibrary.org/obo/OBI_0003142 quantitative binding datum A quantitative binding datum that is expressed as a scalar number and a unit, which can be utilized in equations that model the binding process independent of the specific assay performed. http://purl.obolibrary.org/obo/OBI_0001145 3D structure determination of bound molecular complex assay http://purl.obolibrary.org/obo/OBI_0003117 3D molecular structure determination assay A 3D molecular structure determination assay in which a complex of 2 or more molecular enties is characterized which provides information on their binding configuration. http://purl.obolibrary.org/obo/OBI_0001146 binding assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay with the objective to characterize the disposition of two or more material entities to form a complex. http://purl.obolibrary.org/obo/OBI_0001147 cell culture expansion http://purl.obolibrary.org/obo/OBI_0000094 material processing a process that results in the increase of cell numbers http://purl.obolibrary.org/obo/OBI_0001148 gene knock out http://purl.obolibrary.org/obo/OBI_0600043 genetic transformation a genetic transformation that renders a gene non-functional, e.g. due to a point mutation, or the removal of all, or part of, the gene using recombinant methods. http://purl.obolibrary.org/obo/OBI_0001149 gene knock in http://purl.obolibrary.org/obo/OBI_0600043 genetic transformation a genetic transformation that involves the insertion of a protein coding cDNA sequence at a particular locus in an organism's chromosome. Typically, this is done in mice since the technology for this process is more refined, and because mouse embryonic stem cells are easily manipulated. The difference between knock-in technology and transgenic technology is that a knock-in involves a gene inserted into a specific locus, and is a "targeted" insertion. http://purl.obolibrary.org/obo/OBI_0001151 genetically modified material http://purl.obolibrary.org/obo/OBI_0000047 processed material a material entity, organism or cell, that is the output of a genetic transformation process. http://purl.obolibrary.org/obo/OBI_0001152 transfection http://purl.obolibrary.org/obo/OBI_0600043 genetic transformation a genetic transformation which relies on the use of physical, electrical and chemical phenomena to introduce DNA or RNA into a cell http://purl.obolibrary.org/obo/OBI_0001153 genetic transformation objective http://purl.obolibrary.org/obo/OBI_0000456 material transformation objective a material transformation objective aims to create genetically modified organism or cell http://purl.obolibrary.org/obo/OBI_0001154 induced mutagenesis http://purl.obolibrary.org/obo/OBI_0600043 genetic transformation A genetic transformation that is the modification of the genetic material (either coding or non-coding) of an organism caused by mutagenic processes, compounds, or irradiation. http://purl.obolibrary.org/obo/OBI_0001155 3D structural organization datum http://purl.obolibrary.org/obo/IAO_0000109 measurement datum A measurement datum that describes the structural orientation of a material entity in 3D space. http://purl.obolibrary.org/obo/OBI_0001177 RNA sequencing assay http://purl.obolibrary.org/obo/OBI_0600047 sequencing assay An assay that determines the sequence of an RNA molecule. http://purl.obolibrary.org/obo/OBI_0001181 binding datum http://purl.obolibrary.org/obo/IAO_0000027 data item A data item that states if two or more material entities have the disposition to form a complex, and if so, how strong that disposition is. http://purl.obolibrary.org/obo/OBI_0001199 in vivo design http://purl.obolibrary.org/obo/OBI_0500000 study design A study design that is conducted entirely in a living organism, e.g. a compound treatment in a mouse model. http://purl.obolibrary.org/obo/OBI_0001200 genotyping by high throughput sequencing design http://purl.obolibrary.org/obo/OBI_0001444 genotyping design A study design that classifies an individual or group of individuals on the basis of alleles, haplotypes, SNPs using high througput sequencing techniques. http://purl.obolibrary.org/obo/OBI_0001211 ex vivo design http://purl.obolibrary.org/obo/OBI_0500000 study design A study design where all or part of an organism is removed and studied in vitro, e.g. part of a mouse is removed and cultured in vitro. A cell culture with an established cell line is an in vitro experiment. http://purl.obolibrary.org/obo/OBI_0001225 genetic population background information http://purl.obolibrary.org/obo/OBI_0001404 genetic characteristics information a genetic characteristics information which is a part of genotype information that identifies the population of organisms http://purl.obolibrary.org/obo/OBI_0001234 epigenetic modification identification objective http://purl.obolibrary.org/obo/OBI_0000131 molecular feature identification objective A molecular feature identification objective that aims to detect epigenetic modifications, such as DNA methylation, histone modifications. http://purl.obolibrary.org/obo/OBI_0001239 transcription profiling by high throughput sequencing design http://purl.obolibrary.org/obo/OBI_0500000 study design A study design in which sequencing technology (e.g. Solexa/454) is used to generate RNA sequence, analyse the transcibed regions of the genome, and/or to quantitate transcript abundance http://purl.obolibrary.org/obo/OBI_0001247 genotyping by high throughput sequencing assay http://purl.obolibrary.org/obo/OBI_0000435 genotyping assay A genotyping assay that uses a high througput sequencer http://purl.obolibrary.org/obo/OBI_0001248 ChIP-chip assay http://purl.obolibrary.org/obo/OBI_0001954 ChIP assay An assay in which chromatin is immunoprecipitated and subsequently analyzed using a DNA microarray to identify which parts of DNA are part of the isolated chromatin http://purl.obolibrary.org/obo/OBI_0001258 ChIP-seq design http://purl.obolibrary.org/obo/OBI_0001425 protein binding site identification design A study design which aims to identify protein and DNA interactions using a combination of chromatin immunoprecipitation and high throughput sequencing. Massively parallel sequence analyses are used in conjunction with whole-genome sequence databases to analyze the interaction pattern of any protein with DNA, or the pattern of any epigenetic chromatin modifications. http://purl.obolibrary.org/obo/OBI_0001266 DNA methylation profiling by high throughput sequencing assay http://purl.obolibrary.org/obo/OBI_0000634 DNA methylation profiling assay A DNA methylation profiling assay in which the methylation state of DNA is determined and can be compared between samples using sequencing based technology http://purl.obolibrary.org/obo/OBI_0001271 RNA-seq assay http://purl.obolibrary.org/obo/OBI_0001177 RNA sequencing assay An RNA sequencing assay that determines an RNA sequence by analyzing the transcibed regions of the genome and or to quantitate transcript abundance. http://purl.obolibrary.org/obo/OBI_0001278 DNA methylation profiling by array design http://purl.obolibrary.org/obo/OBI_0500000 study design A study design in which the methylation state of DNA is determined and is compared between samples using array technology. http://purl.obolibrary.org/obo/OBI_0001285 in vitro design http://purl.obolibrary.org/obo/OBI_0500000 study design A study design that is done in a test tube or a culture dish, e.g. A bacterial invasion assay in an established cell culture. http://purl.obolibrary.org/obo/OBI_0001290 transcription profiling by array design http://purl.obolibrary.org/obo/OBI_0500000 study design A study design that identifies forms and abundance of transcripts in the genome using microarray technology. http://purl.obolibrary.org/obo/OBI_0001305 genotype information http://purl.obolibrary.org/obo/OBI_0001404 genetic characteristics information a genetic characteristics information that is about the genetic material of an organism and minimally includes information about the genetic background and can in addition contain information about specific alleles, genetic modifications, etc. http://purl.obolibrary.org/obo/OBI_0001311 X-ray crystallography assay determining the 3D structure of a T cell epitope:MHC:TCR complex http://purl.obolibrary.org/obo/OBI_0001999 3D molecular structure determination assay of a T cell epitope:MHC:TCR complex A X-ray crystallography 3D molecular structure determination assay that characterizes the 3-dimensional molecular structure of a T cell epitope:MHC:TCR complex. http://purl.obolibrary.org/obo/OBI_0001313 transcription profiling by RT-PCR design http://purl.obolibrary.org/obo/OBI_0500000 study design A study design which aims to examine the transcriptome of a biological sample by reverse transcription PCR (RT-PCR). http://purl.obolibrary.org/obo/OBI_0001318 proteomic profiling by array assay http://purl.obolibrary.org/obo/OBI_2100023 protein assay An assay that detects proteins in a sample (quantified or otherwise analyzed), e.g. antibody profiling using an array based technology. http://purl.obolibrary.org/obo/OBI_0001331 transcription profiling identification objective http://purl.obolibrary.org/obo/OBI_0000131 molecular feature identification objective A molecular feature identification objective that aims to characterize the abundance of transcripts http://purl.obolibrary.org/obo/OBI_0001358 post-transcriptional modification design http://purl.obolibrary.org/obo/OBI_0000115 intervention design A study design in which a modification of the transcriptome, proteome (not genome) is made, for example RNAi, antibody targeting. http://purl.obolibrary.org/obo/OBI_0001361 transcription profiling by RT-PCR assay http://purl.obolibrary.org/obo/OBI_0002692 polymerase chain reaction assay A transcription profiling assay that uses reverse transcription PCR (RT-PCR). http://purl.obolibrary.org/obo/OBI_0001364 genetic alteration information http://purl.obolibrary.org/obo/OBI_0001404 genetic characteristics information a genetic characteristics information that is about known changes or the lack thereof from the genetic background, including allele information, duplication, insertion, deletion, etc. http://purl.obolibrary.org/obo/OBI_0001365 cellular process design http://purl.obolibrary.org/obo/OBI_0500000 study design A study design that aims to study the processes that are carried out at the cellular level, but are not necessarily restricted to a single cell. For example, cell communication occurs among more than one cell, but occurs at the cellular level. http://purl.obolibrary.org/obo/OBI_0001396 stimulus or stress design http://purl.obolibrary.org/obo/OBI_0000115 intervention design A study design in which the response of an organism(s) to the stress or stimulus is studied, e.g. osmotic stress, heat shock, radiation exposure, behavioral treatment etc. http://purl.obolibrary.org/obo/OBI_0001398 protein and DNA interaction identification objective http://purl.obolibrary.org/obo/OBI_0000113 sequence feature identification objective A sequence feature identification objective that aims to characterize the interactions between protein and DNA which includes identification of transcription factor binding sites. http://purl.obolibrary.org/obo/OBI_0001403 ChIP-chip design http://purl.obolibrary.org/obo/OBI_0001425 protein binding site identification design A study design which aims to identify protein binding sites in genomic DNA by a combination of chromatin immunoprecipitation and DNA microarray (chip) assays. http://purl.obolibrary.org/obo/OBI_0001404 genetic characteristics information http://purl.obolibrary.org/obo/IAO_0000027 data item a data item that is about genetic material including polymorphisms, disease alleles, and haplotypes. http://purl.obolibrary.org/obo/OBI_0001425 protein binding site identification design http://purl.obolibrary.org/obo/OBI_0500000 study design A study design that investigates protein binding sites on nucleic acids. http://purl.obolibrary.org/obo/OBI_0001430 transcription profiling design http://purl.obolibrary.org/obo/OBI_0500000 study design A study design that identifies forms and abundance of transcripts in the genome. http://purl.obolibrary.org/obo/OBI_0001441 proteomic profiling by array design http://purl.obolibrary.org/obo/OBI_0500000 study design A study design in which proteins in a sample are detected, quantified or otherwise analysed, through an array-based technology. http://purl.obolibrary.org/obo/OBI_0001444 genotyping design http://purl.obolibrary.org/obo/OBI_0500000 study design A study design that classifies an individual or group of individuals on the basis of alleles, haplotypes, SNPs. http://purl.obolibrary.org/obo/OBI_0001460 genetic modification design http://purl.obolibrary.org/obo/OBI_0000115 intervention design A study design in which an organism(s) is studied that has had genetic material removed, rearranged, mutagenized or added, such as in a knock out. http://purl.obolibrary.org/obo/OBI_0001463 transcription profiling by array assay http://purl.obolibrary.org/obo/OBI_0000424 transcription profiling assay A transcription profiling assay that uses array technology. http://purl.obolibrary.org/obo/OBI_0001476 phage display binding assay http://purl.obolibrary.org/obo/OBI_0001146 binding assay A binding assay in which a collection of phages expressing a library of different peptides or protein fragnments is used to infect cells, followed by screening for cells that bind a protein of interest, and identifiying the sequence of infecting phages to determine a suitable binding partner. http://purl.obolibrary.org/obo/OBI_0001479 specimen from organism http://purl.obolibrary.org/obo/OBI_0100051 specimen A specimen that derives from an anatomical part or substance arising from an organism. Examples of tissue specimen include tissue, organ, physiological system, blood, or body location (arm). http://purl.obolibrary.org/obo/OBI_0001480 phage display library panning http://purl.obolibrary.org/obo/OBI_0000094 material processing Phage display library panning is a process in which a diverse collection of phages encoding peptides or proteins are screened and the ones encoding active peptides/proteins are selected in an iterative process similar to natural selection. http://purl.obolibrary.org/obo/OBI_0001501 fluorescence detection assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay in which a material's fluorescence is determined. http://purl.obolibrary.org/obo/OBI_0001505 purification http://purl.obolibrary.org/obo/OBI_0600014 material component separation A planned process to separate a material entity into different compositions of which one or more have are purified fractions that contain higher concentration of a desired component, while others contain impurities and are not of interest http://purl.obolibrary.org/obo/OBI_0001506 specimen with pre- or post-mortem status http://purl.obolibrary.org/obo/OBI_0100051 specimen A specimen that has been established to be taken from a live (pre-mortem) or dead (post-mortem) organism. http://purl.obolibrary.org/obo/OBI_0001553 assay measuring a binding constant of a MHC:ligand complex http://purl.obolibrary.org/obo/OBI_0003145 assay measuring quantitative binding of a MHC:ligand complex A MHC:ligand binding assay that measures a binding constant. http://purl.obolibrary.org/obo/OBI_0001573 DNA sequence data http://purl.obolibrary.org/obo/OBI_0000973 sequence data A sequence data item that is about the primary structure of DNA http://purl.obolibrary.org/obo/OBI_0001588 binding http://purl.obolibrary.org/obo/GO_0008150 biological_process The process of material entities forming complexes. http://purl.obolibrary.org/obo/OBI_0001591 direct binding assay http://purl.obolibrary.org/obo/OBI_0001146 binding assay A binding assay that measures the formation or disassociation of a complex of 2 material entities directly without use of a competitve ligand. http://purl.obolibrary.org/obo/OBI_0001593 competitive inhibition of binding assay http://purl.obolibrary.org/obo/OBI_0001146 binding assay A binding assay that detects the inhibition of binding between 2 material entities known to form a complex by the addition of a third material entity of interest. Inhibition of binding between the 2 materials reflects binding by the third material. http://purl.obolibrary.org/obo/OBI_0001630 analytical chromatography http://purl.obolibrary.org/obo/OBI_0000443 analyte assay An analyte assay that uses a biomaterial's preferential affinity for either the mobile phase or the stationary phase to separate it from other materials and thereby detect its presence in an input material. http://purl.obolibrary.org/obo/OBI_0001631 electron microscopy imaging assay http://purl.obolibrary.org/obo/OBI_0002119 microscopy assay An imaging assay in which an electrons are used to probe the density, shape and composition of an input material which are detected in an electron microscope and utilized to produce an image of the material. http://purl.obolibrary.org/obo/OBI_0001632 immuno staining assay http://purl.obolibrary.org/obo/OBI_0600017 assay detecting a molecular label A assay detecting a molecular label assay in which the label is attached to an antibody so that substances are marked based on the antibody's binding specificity. http://purl.obolibrary.org/obo/OBI_0001633 purified material http://purl.obolibrary.org/obo/OBI_0000047 processed material A material entity that is generated by a purification process in which an input material is separated to obtain a fraction with a higher concentration of a desired component http://purl.obolibrary.org/obo/OBI_0001634 calorimetric binding assay http://purl.obolibrary.org/obo/OBI_0001146 binding assay A binding assay in which the heat generated or absorbed during a binding event is measured, which allows determination of binding constants, reaction stoichiometry, enthalpy and entropy. http://purl.obolibrary.org/obo/OBI_0001635 antibody binding detection by fluorescence quenching http://purl.obolibrary.org/obo/OBI_0001979 fluorescence quenching binding assay A binding assay in which affinity is measured by detecting a change in fluorescence (usually quenching) that occurs upon binding of the antibody to the ligand. The fluorescent signal that is affected by binding is either from an exogenous fluorophore attached to the ligand, or is the intrisic fluorescence of aromatic (tryptophan) residues on the binding site of the antibody (no conjugated fluorophore necessary) or, less commonly, on the ligand binding region (epitope). http://purl.obolibrary.org/obo/OBI_0001668 split-ubiquitin assay http://purl.obolibrary.org/obo/OBI_0001146 binding assay A binding assay that screens membrane soluble proteins by fusion of two integral membrane proteins (bait and prey) to two different ubiquitin moieties. One moiety is also fused to a transcription factor (TF) that can be cleaved off by ubiquitin specific proteases. Upon bait and prey interaction, Nub and Cub-moieties assemble, reconstituting the split-ubiquitin. The reconstituted split-ubiquitin molecule is recognized by ubiquitin specific proteases, which cleave off the reporter protein, allowing it to induce the transcription of reporter genes. http://purl.obolibrary.org/obo/OBI_0001669 far-western blot assay http://purl.obolibrary.org/obo/OBI_0001146 binding assay An assay that measures protein-protein interactions by separating target proteins on an SDS-PAGE gel, transfering them to a solid substrate, hybridizing with a protein probe and visualizing bound proteins using a probe-directed antibody. This is a adaptation on the western blot assay. http://purl.obolibrary.org/obo/OBI_0001670 RNA protection assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that determines the presence and estimates abundance of transcript species by first creating an homo or heteroduplex by adding a specific, complementary sequence to the sequence of interest and then exposing the mixture of ribonuclease, which will degrade only single stranded molecules. A detection step will reveal if the sample contained a sequence of interest. http://purl.obolibrary.org/obo/OBI_0001671 electrophoretic mobility shift assay http://purl.obolibrary.org/obo/OBI_0001146 binding assay An assay that measures information about Protein-DNA or Protein-RNA interactions using gel electrophoresis and relying on the fact the molecular interactions will cause the heterodimer to be retarded on the gel when compared to controls corresponding to protein extract alone and protein extract + neutral nucleic acid. http://purl.obolibrary.org/obo/OBI_0001672 gene knock-down assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay which transiently disrupts gene transcripts by expressing antisense RNA constructs or delivering RNA interfering molecules in cells. http://purl.obolibrary.org/obo/OBI_0001673 nano-cap analysis of gene expression assay http://purl.obolibrary.org/obo/OBI_0000424 transcription profiling assay A transcription profiling assay that is performed using a very low amount (nanogram scale) of mRNA samples using Cap analysis gene expression (CAGE). http://purl.obolibrary.org/obo/OBI_0001674 cap analysis of gene expression assay http://purl.obolibrary.org/obo/OBI_0002692 polymerase chain reaction assay A transcription profiling assay which measures RNA transcript abundances in biological samples by extracting 5' ends of capped transcripts, RTPCR and sequence those. Copy numbers of CAGE tags provide a way of quantification and provide a measure of expression of the transcriptome http://purl.obolibrary.org/obo/OBI_0001679 yeast 2-hybrid assay http://purl.obolibrary.org/obo/OBI_0001146 binding assay An assay that detects protein protein interactions and protein DNA interactions by testing for physical interactions (such as binding) between two proteins or a single protein and a DNA molecule, respectively. The premise behind the test is the activation of downstream reporter gene(s) by the binding of a transcription factor onto an upstream activating sequence (UAS). For two-hybrid screening, the transcription factor is split into two separate fragments, called the binding domain (BD) and activating domain (AD). The BD is the domain responsible for binding to the UAS and the AD is the domain responsible for the activation of transcription. The Y2H is thus a protein-fragment complementation assay. http://purl.obolibrary.org/obo/OBI_0001681 yeast one-hybrid assay http://purl.obolibrary.org/obo/OBI_0001146 binding assay An assay that determines protein DNA interactions using a single fusion protein in which the activating domain is linked directly to the binding domain. http://purl.obolibrary.org/obo/OBI_0001682 bacterial one-hybrid assay http://purl.obolibrary.org/obo/OBI_0001681 yeast one-hybrid assay An assay that identifies the sequence-specific target site of a DNA-binding domain. In this system, a given transcription factor (TF) is expressed as a fusion to a subunit of RNA polymerase. In parallel, a library of randomized oligonucleotides representing potential TF target sequences, is cloned into a separate vector containing the selectable genes HIS3 and URA3. If the DNA-binding domain (bait) binds a potential DNA target site (prey) in vivo, it will recruit RNA polymerase to the promoter and activate transcription of the reporter genes in that clone. The two reporter genes, HIS3 and URA3, allow for positive and negative selections, respectively. At the end of the process, positive clones are sequenced and examined with motif-finding tools in order to resolve the favoured DNA target sequence http://purl.obolibrary.org/obo/OBI_0001683 chromosome organization assay by fluorescence in-situ hybridization http://purl.obolibrary.org/obo/OBI_0001686 in-situ hybridization assay An in-situ hybridization assay that uses fluorescence to detect chromosomal integrity http://purl.obolibrary.org/obo/OBI_0001684 methylation-specific polymerase chain reaction assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that uses initial modification of DNA by sodium bisulfite, converting all unmethylated, but not methylated, cytosines to uracil, and subsequent amplification with primers specific for methylated versus unmethylated DNA. http://purl.obolibrary.org/obo/OBI_0001685 amplification of intermethylated sites assay http://purl.obolibrary.org/obo/OBI_0000634 DNA methylation profiling assay An assay that estimates genome-wide DNA methylation and measures methylation of DNA sequences. AIMS is based on the differential enzymatic digestion of genomic DNA with methylation-sensitive and methylation-insensitive isoschizomers followed by restrained PCR amplification of methylated sequences. http://purl.obolibrary.org/obo/OBI_0001686 in-situ hybridization assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that localizes a specific DNA or RNA sequence within a portion or section of tissue using artificially induced nucleic hybridization. http://purl.obolibrary.org/obo/OBI_0001689 cytochalasin-induced inhibition of actin polymerization assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay which uses compound cytochalasin (CHEBI: 23528) to block actin polymerization-dependent cell motility (GO:0070358) and actin filament polymerization (GO:0030041). http://purl.obolibrary.org/obo/OBI_0001691 cellular structure feature identification objective http://purl.obolibrary.org/obo/OBI_0000015 biological feature identification objective is an objective specification which aims to discover cellular structure properties http://purl.obolibrary.org/obo/OBI_0001700 immunoprecipitation assay http://purl.obolibrary.org/obo/OBI_0000443 analyte assay An analyte assay in which an input material is mixed with antibodies and bound antigen:antibody complexes are separated out using immunoprecipitation. Either the antibody has known specificy, and the antigen mixture is tested for the presence of a specific antigen, or the antigen solution is well defined and the antibody solution is tested for the presence of antigen specific antibodies. http://purl.obolibrary.org/obo/OBI_0001702 immunoglobulin binding to epitope http://purl.obolibrary.org/obo/OBI_1110014 epitope binding by adaptive immune receptor a process of an immunoglobulin complex binding to a material entity at the immunoglobulin complementarity determining region (CDR). http://purl.obolibrary.org/obo/OBI_0001706 assay measuring biological activity resulting from B cell epitope:antibody binding http://purl.obolibrary.org/obo/OBI_0003127 B cell epitope assay A B cell epitope assay that measures the immune response process resulting from the binding of an antibody receptor to epitope or recognition of the epitope. http://purl.obolibrary.org/obo/OBI_0001709 assay measuring a binding constant of a B cell epitope:antibody complex http://purl.obolibrary.org/obo/OBI_0003146 assay measuring quantitative binding of an antigen:antibody complex A B cell epitope recognition assay that quantitavely characterizes the binding of an antibody / BCR with a ligand by determining a binding constant. http://purl.obolibrary.org/obo/OBI_0001721 3D molecular structure determination assay of an antigen:antibody complex http://purl.obolibrary.org/obo/OBI_0003127 B cell epitope assay A 3D structure determination of bound molecular complex assay that characterizes the 3-dimensional structure of an antigen:antobody complex. http://purl.obolibrary.org/obo/OBI_0001738 X-ray crystallography assay determining the 3D structure of a B cell epitope:antibody complex http://purl.obolibrary.org/obo/OBI_0001721 3D molecular structure determination assay of an antigen:antibody complex A X-ray crystallography 3D molecular structure determination assay that characterizes the 3-dimensional molecular structrue of a B cell epitope:antibody complex. http://purl.obolibrary.org/obo/OBI_0001852 paired-end library preparation http://purl.obolibrary.org/obo/OBI_0000711 library preparation A library preparation that results in the creation of a library of the 5' and 3' ends of DNA or cDNA fragments using adaptors and endonucleases. The preparation may or may not include cloning process. http://purl.obolibrary.org/obo/OBI_0001861 methylation-sensitive restriction enzyme sequencing assay http://purl.obolibrary.org/obo/OBI_0001266 DNA methylation profiling by high throughput sequencing assay An assay that identifies unmethylated CpGs using methylation sensitive restriction enzymes to fragment DNA. http://purl.obolibrary.org/obo/OBI_0001865 assay array http://purl.obolibrary.org/obo/OBI_0000968 device A device made to be used in an analyte assay for immobilization of substances that bind the analyte at regular spatial positions on a surface. http://purl.obolibrary.org/obo/OBI_0001879 reagent http://purl.obolibrary.org/obo/OBI_0000047 processed material A biological or chemical entity that bears a reagent role in virtue of it being intended for application in a scientific technique to participate in (or have molecular parts that participate in) a chemical reaction that facilitates the generation of data about some distinct entity, or the generation of some distinct material specified output. http://purl.obolibrary.org/obo/OBI_0001909 conclusion based on data http://purl.obolibrary.org/obo/IAO_0000030 information content entity An information content entity that is inferred from data. http://purl.obolibrary.org/obo/OBI_0001912 cell freezing medium http://purl.obolibrary.org/obo/OBI_0000047 processed material A processed material that serves as a liquid vehicle for freezing cells for long term quiescent stroage, which contains chemicls needed to sustain cell viability across freeze-thaw cycles. http://purl.obolibrary.org/obo/OBI_0001914 multiplex ligation-mediated amplification http://purl.obolibrary.org/obo/OBI_0000415 polymerase chain reaction A polymerase chain reaction that amplifies multiple targets with a single primer pair mediated by hybridization of a primer with its target sequence using ligation. http://purl.obolibrary.org/obo/OBI_0001917 chromosome conformation identification objective http://purl.obolibrary.org/obo/OBI_0000131 molecular feature identification objective A molecular feature identification objective that aims to determine spatial organization of chromatin. http://purl.obolibrary.org/obo/OBI_0001919 Carbon-copy chromosome conformation capture assay http://purl.obolibrary.org/obo/OBI_0002692 polymerase chain reaction assay An assay that measures the organization of chromosomes at the genome-wide scale. http://purl.obolibrary.org/obo/OBI_0001925 chromatin immunoprecipitation with exonuclease sequencing assay http://purl.obolibrary.org/obo/OBI_0000716 ChIP-seq assay A ChIP-seq assay which uses immunoprecipitation to isolate protein bound DNA followed by an exonuclease step to degrade DNA that is not protein bound to provide greater resolution of the DNA binding site http://purl.obolibrary.org/obo/OBI_0001931 scalar value specification http://purl.obolibrary.org/obo/OBI_0001933 value specification A value specification that consists of two parts: a numeral and a unit label http://purl.obolibrary.org/obo/OBI_0001933 value specification http://purl.obolibrary.org/obo/IAO_0000030 information content entity An information content entity that specifies a value within a classification scheme or on a quantitative scale. http://purl.obolibrary.org/obo/OBI_0001936 molecular-labeled material http://purl.obolibrary.org/obo/OBI_0000047 processed material a material entity that is the specified output of an addition of molecular label process that aims to label some molecular target to allow for its detection in a detection of molecular label assay http://purl.obolibrary.org/obo/OBI_0001954 ChIP assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay in which portions of chromatin, the ordered and organized complex of DNA and its interaction partners that make up a chormosome, is extracted and purified by immunoprecipitation with antibodies or tags, and subsequently analyzed http://purl.obolibrary.org/obo/OBI_0001955 competitive binding reference ligand role http://purl.obolibrary.org/obo/OBI_0000064 positive reference substance role A positive reference substance role that inheres in a material entity that is known to bind to a target entity, and that is realized in a competitive binding assay that has as specified input the target entity, an evaluant and the positive reference substance, where the binding of the evaluant to the target is measured based on the evaluant's ability to compete with the positive reference substance for binding to the target. http://purl.obolibrary.org/obo/OBI_0001956 assay using chromatin immunoprecipitation http://purl.obolibrary.org/obo/OBI_0001700 immunoprecipitation assay An assay that produces data about protein-DNA interaction or DNA epigenetic modification using immunoprecipitation http://purl.obolibrary.org/obo/OBI_0001977 cytometry assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that counts and/or measures characteristics of cells. http://purl.obolibrary.org/obo/OBI_0001978 immunoblot assay http://purl.obolibrary.org/obo/OBI_0000443 analyte assay An analyte assay that detects specific molecules in an input material by separating it using gel electrophoresis, transfering the separated molecules to a membrane, and staining them with_ antibodies specific to the analyte molecules. http://purl.obolibrary.org/obo/OBI_0001979 fluorescence quenching binding assay http://purl.obolibrary.org/obo/OBI_0001146 binding assay A binding assay in which the proximity of two entities is monitored by measuring a fluorescent signal of one of the entities that gets reduced if the two entities are cliose to each other. http://purl.obolibrary.org/obo/OBI_0001985 microarray assay http://purl.obolibrary.org/obo/OBI_0000443 analyte assay An analyte assay where binding of the analyte to immobilized matrix is measured. http://purl.obolibrary.org/obo/OBI_0001986 immunohistochemistry http://purl.obolibrary.org/obo/OBI_0001632 immuno staining assay An immunostaining assay to detect and potentially localize antigens within the cells of a tissue section. http://purl.obolibrary.org/obo/OBI_0001999 3D molecular structure determination assay of a T cell epitope:MHC:TCR complex http://purl.obolibrary.org/obo/OBI_0003128 T cell epitope assay A 3D structure determination of bound molecular complex assay that characterizes the 3-dimensional structure of a T cell epitope:MHC:TCR complex. http://purl.obolibrary.org/obo/OBI_0002020 epigenetic modification assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that identifies epigenetic modifications including histone modifications, open chromatin, and DNA methylation. http://purl.obolibrary.org/obo/OBI_0002035 peptide mass fingerprinting assay http://purl.obolibrary.org/obo/OBI_0000470 mass spectrometry assay A mass spectrometry assay that measures the absolute mass of the cleaved peptides derived from an unknown protein of interest. These masses are then compared to values for known protein sequences to identify the unknown protein. http://purl.obolibrary.org/obo/OBI_0002050 3D molecular structure determination assay of a MHC:ligand complex http://purl.obolibrary.org/obo/OBI_0002075 MHC ligand assay A 3D structure determination of bound molecular complex assay that characterizes the 3-dimensional structure of a MHC:ligand complex. http://purl.obolibrary.org/obo/OBI_0002055 assay measuring biological activity resulting from T cell epitope:MHC:TCR binding http://purl.obolibrary.org/obo/OBI_0003128 T cell epitope assay A T cell epitope recognition assay that measures the immune response process resulting from the binding of a T cell receptor to epitope or the recongition of the epitope. http://purl.obolibrary.org/obo/OBI_0002059 assay measuring a binding constant of a T cell epitope:MHC:TCR complex http://purl.obolibrary.org/obo/OBI_0003147 assay measuring quantitative binding of an epitope:MHC:TCR complex A T cell epitope recognition assay that quantitavely characterizes the binding of a TCR with a ligand by determining a binding constant. http://purl.obolibrary.org/obo/OBI_0002075 MHC ligand assay http://purl.obolibrary.org/obo/OBI_1110128 immune epitope assay An immune epitope assay that characterizes the structrue of a MHC-ligand complex, or detects the processing and presentation of a ligand by an antigen presenting cell, or the binding of a ligand to an MHC molecule. http://purl.obolibrary.org/obo/OBI_0002076 collection of specimens http://purl.obolibrary.org/obo/BFO_0000040 material entity A material entity that has two or more specimens as its parts. http://purl.obolibrary.org/obo/OBI_0002082 reporter gene assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that detects expression of a reporter gene that was inserted under the control of a regulatory sequence of interest. http://purl.obolibrary.org/obo/OBI_0002089 physical store http://purl.obolibrary.org/obo/OBI_0001034 environmental control device A container with an environmental control function. http://purl.obolibrary.org/obo/OBI_0002090 rapid amplification of cDNA ends http://purl.obolibrary.org/obo/OBI_0000552 reverse transcribed polymerase chain reaction A reverse transcribed polymerase chain reaction that is used to amplify a mRNA sequence between a defined internal site and the 5' or 3' end of the mRNA. http://purl.obolibrary.org/obo/OBI_0002116 high performance liquid chromotography assay http://purl.obolibrary.org/obo/OBI_0001630 analytical chromatography An analytical chromatography assay that utilizes a high performance liquid chromatography instrument for separation of compounts in a solution. http://purl.obolibrary.org/obo/OBI_0002119 microscopy assay http://purl.obolibrary.org/obo/OBI_0000185 imaging assay An imaging assay that utilizes a microscope to magnify features of the visualized material of interest that are not visible to naked eye. http://purl.obolibrary.org/obo/OBI_0002516 brain specimen http://purl.obolibrary.org/obo/OBI_0001479 specimen from organism A specimen that is derived from brain. http://purl.obolibrary.org/obo/OBI_0100010 Epstein Barr virus transformed B cell http://purl.obolibrary.org/obo/OBI_0000047 processed material A material entity which results from viral transformation process using EBV as transformation agent when applied to B-cell entity http://purl.obolibrary.org/obo/OBI_0100026 organism http://purl.obolibrary.org/obo/BFO_0000040 material entity A material entity that is an individual living system, such as animal, plant, bacteria or virus, that is capable of replicating or reproducing, growth and maintenance in the right environment. An organism may be unicellular or made up, like humans, of many billions of cells divided into specialized tissues and organs. http://purl.obolibrary.org/obo/OBI_0100051 specimen http://purl.obolibrary.org/obo/BFO_0000040 material entity A material entity that has the specimen role. http://purl.obolibrary.org/obo/OBI_0100060 cultured cell population http://purl.obolibrary.org/obo/OBI_0000047 processed material A processed material comprised of a collection of cultured cells that has been continuously maintained together in culture and shares a common propagation history. http://purl.obolibrary.org/obo/OBI_0100066 organ section http://purl.obolibrary.org/obo/OBI_0000047 processed material A processed material which derives from an organ and results from a process of dissection or histological sample preparation a portion(formerly an organ section is portion of an organ removed from the context of the organ) http://purl.obolibrary.org/obo/OBI_0200000 data transformation http://purl.obolibrary.org/obo/OBI_0000011 planned process A planned process that produces output data from input data. http://purl.obolibrary.org/obo/OBI_0200031 differential expression analysis objective http://purl.obolibrary.org/obo/OBI_0200166 data transformation objective A differential expression analysis objective is a data transformation objective whose input consists of expression levels of entities (such as transcripts or proteins), or of sets of such expression levels, under two or more conditions and whose output reflects which of these are likely to have different expression across such conditions. http://purl.obolibrary.org/obo/OBI_0200085 mass spectrometry analysis http://purl.obolibrary.org/obo/OBI_0200000 data transformation A data transformation which has the objective of spectrum analysis. http://purl.obolibrary.org/obo/OBI_0200166 data transformation objective http://purl.obolibrary.org/obo/IAO_0000005 objective specification An objective specification to transformation input data into output data http://purl.obolibrary.org/obo/OBI_0200197 spectrum analysis objective http://purl.obolibrary.org/obo/OBI_0200166 data transformation objective is a data transformation objective where the aim is to analyse some aspect of spectral data by some data transformation process. http://purl.obolibrary.org/obo/OBI_0302716 pool of specimens http://purl.obolibrary.org/obo/OBI_0000953 processed specimen A pool of specimens is a mixture of a population of samples which have been gathered from one or more sample populations, obtained by the physical process of mixing individual specimens, e.g. mixing the DNA collected from the individual fish. http://purl.obolibrary.org/obo/OBI_0302729 chemical solution http://purl.obolibrary.org/obo/BFO_0000040 material entity A material entity that is made up of at least 2 scattered molecular aggregates, one playing the role of solvent and the other one playing the role of solute. http://purl.obolibrary.org/obo/OBI_0302732 solvent role http://purl.obolibrary.org/obo/BFO_0000023 role solvent role is a role which inheres in a molecular entity capable of ensuring the dissolution of another chemical entity and realized by the process of solvation http://purl.obolibrary.org/obo/OBI_0302733 solute role http://purl.obolibrary.org/obo/BFO_0000023 role solute role is a role played by a chemical entity which is dissolved by another chemical entity (the solvent) when creating a solution http://purl.obolibrary.org/obo/OBI_0302736 comet assay http://purl.obolibrary.org/obo/OBI_0001977 cytometry assay An assay that measures DNA damage (DNA breakage) in eucaryotic cells exposed to a challenge by determining the size and shape of DNA migration by detecting fluorescently labeled DNA from a cell placed in an electric field using gel electrophoresis http://purl.obolibrary.org/obo/OBI_0302859 genetically modified organism http://purl.obolibrary.org/obo/OBI_0100026 organism an organism that is the output of a genetic transformation process http://purl.obolibrary.org/obo/OBI_0302876 dissolved material entity http://purl.obolibrary.org/obo/OBI_0000576 scattered molecular aggregate A material entity that has been going through a process of being put into solution http://purl.obolibrary.org/obo/OBI_0302884 extraction http://purl.obolibrary.org/obo/OBI_0600014 material component separation A material separation in which a desired component of an input material is separated from the remainder http://purl.obolibrary.org/obo/OBI_0302887 staining http://purl.obolibrary.org/obo/OBI_0000094 material processing Staining is a process which results in the addition a class-specific (DNA, proteins, lipids, carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. http://purl.obolibrary.org/obo/OBI_0302890 polymerization http://purl.obolibrary.org/obo/OBI_0000094 material processing polymerization is process by which molecular entity of small mass are aggregated in motifs over the course of a chemical reaction catalyzed by enzymes or other molecular entities acting as catalyst. polymerization results in molecular entity of high molecular weight http://purl.obolibrary.org/obo/OBI_0302892 enzymatic ligation http://purl.obolibrary.org/obo/OBI_0000094 material processing An enzymatic ligation is a planned process in which molecules are joined by covalent bonds through the action of an material entity with a ligase activity http://purl.obolibrary.org/obo/OBI_0302903 nucleic acid hybridization http://purl.obolibrary.org/obo/OBI_0000094 material processing a planned process by which totally or partially complementary, single-stranded nucleic acids are combined into a single molecule called heteroduplex or homoduplex to an extent depending on the amount of complementarity. http://purl.obolibrary.org/obo/OBI_0302905 elution http://purl.obolibrary.org/obo/OBI_0000094 material processing the process of extracting one material from another by washing with a solvent to remove adsorbed material from an adsorbent (as in washing of loaded ion-exchange resins to remove captured ions) http://purl.obolibrary.org/obo/OBI_0400043 flow cell http://purl.obolibrary.org/obo/OBI_0000967 container Aparatus in the fluidic subsystem where the sheath and sample meet. Can be one of several types; jet-in-air, quartz cuvette, or a hybrid of the two. The sample flows through the center of a fluid column of sheath fluid in the flow cell. http://purl.obolibrary.org/obo/OBI_0400044 flow cytometer http://purl.obolibrary.org/obo/OBI_0400137 cytometer A flow_cytometer is an instrument for counting, examining and sorting microscopic particles in suspension. It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of single cells flowing through an optical and/or electronic detection apparatus. A flow cytometer is an instrument that can be used to quantitatively measure the properties of individual cells in a flowing medium. http://purl.obolibrary.org/obo/OBI_0400065 light source http://purl.obolibrary.org/obo/OBI_0001032 light emission device A light source is an optical subsystem that provides light for use in a distant area using a delivery system (e.g., fiber optics). Light sources may include one of a variety of lamps (e.g., xenon, halogen, mercury). Most light sources are operated from line power, but some may be powered from batteries. They are mostly used in endoscopic, microscopic, and other examination and/or in surgical procedures. The light source is part of the optical subsystem. In a flow cytometer the light source directs high intensity light at particles at the interrogation point. The light source in a flow cytometer is usually a laser. http://purl.obolibrary.org/obo/OBI_0400078 obscuration bar http://purl.obolibrary.org/obo/OBI_0000932 material separation device An obscuration bar is a an optical subsystem which is a strip of metal or other material that serves to block out direct light from the illuminating beam. The obscuration bar prevents the bright light scattered in the forward directions from burning out the collection device. http://purl.obolibrary.org/obo/OBI_0400079 optical filter http://purl.obolibrary.org/obo/OBI_0000932 material separation device An optical filter is an optical subsystem that selectively transmits light having certain properties (often, a particular range of wavelengths, that is, range of colours of light), while blocking the remainder. They are commonly used in photography, in many optical instruments, and to colour stage lighting Optical filters can be arranged to segregate and collect light by wave length. http://purl.obolibrary.org/obo/OBI_0400082 photodetector http://purl.obolibrary.org/obo/OBI_0000832 measurement device A photodetector is a device used to detect and measure the intensity of radiant energy through photoelectric action. In a cytometer, photodetectors measure either the number of photons of laser light scattered on impact with a cell (for example), or the flourescence emitted by excitation of a fluorescent dye. http://purl.obolibrary.org/obo/OBI_0400103 DNA sequencer http://purl.obolibrary.org/obo/OBI_0000832 measurement device A DNA sequencer is an instrument that determines the order of deoxynucleotides in deoxyribonucleic acid sequences. http://purl.obolibrary.org/obo/OBI_0400110 hybridization chamber http://purl.obolibrary.org/obo/OBI_0002089 physical store A device which is used to maintain constant contact of a liquid on an array. This can be either a glass vial or slide. http://purl.obolibrary.org/obo/OBI_0400114 sonicator http://purl.obolibrary.org/obo/OBI_0001033 perturbation device A device that converts a variable electrical current to mechanical vibration of a metallic probe. The device is used for the lysis of cells, the mixing of compounds or solutions, to framgent molecules of DNA, or to create emulsions. http://purl.obolibrary.org/obo/OBI_0400115 spectrophotometer http://purl.obolibrary.org/obo/OBI_0000832 measurement device A spectrophotometer is an instrument that measures the intensity of light as a function of the color, or more specifically, the wavelength of light, transmitted by a substance. http://purl.obolibrary.org/obo/OBI_0400116 thermal cycler http://purl.obolibrary.org/obo/OBI_0002089 physical store An instrument that is capable of repeatedly altering and maintaining specific temperatures for defined periods of time. http://purl.obolibrary.org/obo/OBI_0400137 cytometer http://purl.obolibrary.org/obo/OBI_0000832 measurement device A cytometer is an instrument for counting and/or measuring characteristics of cells. http://purl.obolibrary.org/obo/OBI_0400140 gel tank http://purl.obolibrary.org/obo/OBI_0002089 physical store A device which holds a gel and running buffers to allow electrophoresis to be performed. A gel tank has the function to contain and to control the contained environment and transfer energy from an energy supply through the running buffers to the gel matrix and the material with charged molecules in an electric field across a porous matrix or medium with the objective to separate the charged molecules. http://purl.obolibrary.org/obo/OBI_0400142 power supply http://purl.obolibrary.org/obo/OBI_0000968 device A power supply is an device or part of a device that permits the required application of a defined electrical charge to an instrument. The power supply may permit the defined application of a given amount of current for a defined length of time. http://purl.obolibrary.org/obo/OBI_0400147 microarray http://purl.obolibrary.org/obo/OBI_0001865 assay array A processed material that is made to be used in an analyte assay. It consists of a physical immobilisation matrix in which substances that bind the analyte are placed in regular spatial position. http://purl.obolibrary.org/obo/OBI_0400148 DNA microarray http://purl.obolibrary.org/obo/OBI_0400147 microarray A DNA-microarray is a microarray that is used as a physical 2D immobilisation matrix for DNA sequences. DNA microarray-bound DNA fragments are used as targets for a hybridising probed sample. http://purl.obolibrary.org/obo/OBI_0400149 protein microarray http://purl.obolibrary.org/obo/OBI_0400147 microarray A protein-microarray is a microarray, ususlly a piece of glass, on which different molecules of protein have been affixed at separate locations in an ordered manner. These are used to identify protein-protein or protein-small molecule interactions. http://purl.obolibrary.org/obo/OBI_0400153 droplet sorter http://purl.obolibrary.org/obo/OBI_0000932 material separation device A droplet sorter is part_of a flow cytometer sorter that converts the carrier fluid stream into individual droplets, and these droplets are directed into separate locations for recovery (enriching the original sample for particles of interest based on qualities determined by gating) or disposal. http://purl.obolibrary.org/obo/OBI_0400168 microtome http://purl.obolibrary.org/obo/OBI_0000932 material separation device A microtome is a mechanical instrument used to cut biological specimens into very thin segments for further treatment (e.g. ISH) and ultimately microscopic or histologic examination. Most microtomes provide cooling facilities (cryo-microtome) and use a steel blade to cut a slice of defined thickness. Some are automatic, and some are driven by hand. http://purl.obolibrary.org/obo/OBI_0400169 microscope http://purl.obolibrary.org/obo/OBI_0000398 image creation device A microscope is an instrument which magnifies the view on objects (too small to be viewed by the naked eye) under increased resolution. A microscope can be an optical instrument but also and electronic instrument. There are various kind of optical microscopes, e.g confocal microscope, epifluoresence microscope) http://purl.obolibrary.org/obo/OBI_0500000 study design http://purl.obolibrary.org/obo/IAO_0000104 plan specification A plan specification comprised of protocols (which may specify how and what kinds of data will be gathered) that are executed as part of an investigation and is realized during a study design execution. http://purl.obolibrary.org/obo/OBI_0500001 clinical study design http://purl.obolibrary.org/obo/OBI_0500000 study design Plan for the precise procedure to be followed in a clinical trial, including planned and actual timing of events, choice of control group, method of allocating treatments, blinding methods; assigns a subject to pass through one or more epochs in the course of a trial. Specific design elements, e.g., crossover, parallel; dose-escalation [Modified from Pocock, Clinical Trials: A Practical Approach] http://purl.obolibrary.org/obo/OBI_0500010 reference design http://purl.obolibrary.org/obo/OBI_0500000 study design A reference experiment design type is where all samples are compared to a common reference. http://purl.obolibrary.org/obo/OBI_0600000 adding substance to cell culture http://purl.obolibrary.org/obo/OBI_0000274 adding a material entity into a target is process in which a material substance is added to a cell culture http://purl.obolibrary.org/obo/OBI_0600005 collecting specimen from organism http://purl.obolibrary.org/obo/OBI_0000659 specimen collection process a process with the objective to obtain a material entity that was part of an organism for potential future use in an investigation http://purl.obolibrary.org/obo/OBI_0600007 administering substance in vivo http://purl.obolibrary.org/obo/OBI_0000274 adding a material entity into a target A process by which a substance is intentionally given to an organism resulting in exposure of the organism to that substance. http://purl.obolibrary.org/obo/OBI_0600014 material component separation http://purl.obolibrary.org/obo/OBI_0000094 material processing a material processing in which components of an input material become segregated in space http://purl.obolibrary.org/obo/OBI_0600017 assay detecting a molecular label http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that detects the presence or a quality of a molecular label which is a proxy for the detection of the molecular target to which the label is attached http://purl.obolibrary.org/obo/OBI_0600020 histological assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that uses visual examination of cells or tissue (or images of them) to make an assessment regarding a quality of the cells or tissue. This assay can include steps of staining, imaging, and judgement. http://purl.obolibrary.org/obo/OBI_0600024 maintaining cell culture http://purl.obolibrary.org/obo/OBI_0000838 material maintenance a protocol application in which cells are kept alive in a defined environment outside of an organism. part of cell_culturing http://purl.obolibrary.org/obo/OBI_0600025 substance detection assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that detects a specified substance http://purl.obolibrary.org/obo/OBI_0600028 artificially induced reverse transcription http://purl.obolibrary.org/obo/OBI_0000094 material processing a protocol with the objective to transcribe single-stranded RNA into complementary DNA (cDNA) http://purl.obolibrary.org/obo/OBI_0600033 cell permeabilization http://purl.obolibrary.org/obo/OBI_0000094 material processing A protocol application to permeabilize cell membranes, allowing molecules to more easily pass through the membrane than was possible prior to the protocol application http://purl.obolibrary.org/obo/OBI_0600036 establishing cell culture http://purl.obolibrary.org/obo/OBI_0000094 material processing a process through which a new type of cell culture or cell line is created, either through the isolation and culture of one or more cells from a fresh source, or the deliberate experimental modification of an existing cell culture (e.g passaging a primary culture to become a secondary culture or line, or the immortalization or stable genetic modification of an existing culture or line). http://purl.obolibrary.org/obo/OBI_0600038 addition of molecular label http://purl.obolibrary.org/obo/OBI_0000094 material processing a material processing technique intended to add a molecular label to some input material entity, to allow detection of the molecular target of this label in a detection of molecular label assay http://purl.obolibrary.org/obo/OBI_0600043 genetic transformation http://purl.obolibrary.org/obo/OBI_0000094 material processing the introduction. alteration or integration of genetic material into a cell or organism http://purl.obolibrary.org/obo/OBI_0600045 3D structure determination assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that determines the 3-dimensional configuration of an input material. http://purl.obolibrary.org/obo/OBI_0600046 preparative chromatography http://purl.obolibrary.org/obo/OBI_0600014 material component separation the use of a biomaterial's preferential affinity for either the mobile phase or the stationary phase to separate it from other materials of differing affinity http://purl.obolibrary.org/obo/OBI_0600047 sequencing assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay the uses chemical or biochemical means to infer the sequence of a biomaterial http://purl.obolibrary.org/obo/OBI_0600050 specific enzymatic cleavage http://purl.obolibrary.org/obo/OBI_0000231 enzymatic cleavage an enzymatic cleavage that uses an enzyme with a strong affinity for specific cleavage sites in the input material http://purl.obolibrary.org/obo/OBI_0600051 gradient separation http://purl.obolibrary.org/obo/OBI_0600014 material component separation a protocol application that uses different concentrations of materials in a defined order to create a gradient to facilitate the separation of an input material into its components with specific qualities http://purl.obolibrary.org/obo/OBI_0600053 electrophoresis http://purl.obolibrary.org/obo/OBI_0600014 material component separation a protocol application that uses an electrical potential to move material through a defined matrix in order to separate it by its resistance to movement and its charge http://purl.obolibrary.org/obo/OBI_0600054 selection by survival http://purl.obolibrary.org/obo/OBI_0000094 material processing the use of environmental conditions to select for the organism or cells that have a certain trait http://purl.obolibrary.org/obo/OBI_0600055 DNA restriction enzyme digestion http://purl.obolibrary.org/obo/OBI_0600050 specific enzymatic cleavage A specific enzymatic cleavage of DNA molecules using endonucleases that cleave DNA molecules at specific sites based on the surrounding nucleotide sequence http://purl.obolibrary.org/obo/OBI_0600058 enzymatic amplification http://purl.obolibrary.org/obo/OBI_0000094 material processing the use of enzymes to increase the number of molecules of a biomaterial http://purl.obolibrary.org/obo/OBI_0600064 recombinant vector cloning http://purl.obolibrary.org/obo/OBI_0000094 material processing a planned process with the objective to insert genetic material into a cloning vector for future replication of the inserted material http://purl.obolibrary.org/obo/OBI_0666666 RNA extraction http://purl.obolibrary.org/obo/OBI_0666667 nucleic acid extraction A RNA extraction is a nucleic acid extraction where the desired output material is RNA http://purl.obolibrary.org/obo/OBI_0666667 nucleic acid extraction http://purl.obolibrary.org/obo/OBI_0302884 extraction a material separation to recover the nucleic acid fraction of an input material http://purl.obolibrary.org/obo/OBI_1000029 phage display library http://purl.obolibrary.org/obo/OBI_0000731 recombinant vector a phage display library is a collection of materials in which a mixture of genes or gene fragments is expressed and can be individually selected and amplified. http://purl.obolibrary.org/obo/OBI_1000048 transgenic organism http://purl.obolibrary.org/obo/OBI_0302859 genetically modified organism a transgenic organism is material entity which derives from an organism which has been modified to express a gene not normally part of it http://purl.obolibrary.org/obo/OBI_1110001 epitope http://purl.obolibrary.org/obo/BFO_0000040 material entity a material entity bearing the epitope role http://purl.obolibrary.org/obo/OBI_1110014 epitope binding by adaptive immune receptor http://purl.obolibrary.org/obo/GO_0008150 biological_process is the process in which an adaptive immune receptor binds to a material entity (realizing its disposition). The binding affinity is significant enough to trigger an immune response. Specifically, transient non-specific binding of adaptive immune receptors occurring during immune surveillance is not considered significant binding. http://purl.obolibrary.org/obo/OBI_1110021 infection process http://purl.obolibrary.org/obo/OBI_1110122 pathologic process the detrimental process in which an infectious agent colonizes or replicates in a host environment http://purl.obolibrary.org/obo/OBI_1110022 adaptive immune receptor http://purl.obolibrary.org/obo/GO_0032991 protein-containing complex is a receptor produced by cells of the adaptive immune system with the purpose of binding epitopes. http://purl.obolibrary.org/obo/OBI_1110030 experimental infection of cell culture http://purl.obolibrary.org/obo/OBI_0600000 adding substance to cell culture is the administration of an infectious agent to a cell culture with the objective to have the agent colonize and replicate in culture http://purl.obolibrary.org/obo/OBI_1110034 antigen http://purl.obolibrary.org/obo/BFO_0000040 material entity is a material entity that has the antigen role http://purl.obolibrary.org/obo/OBI_1110037 assay measuring binding of a T cell epitope:MHC:TCR complex http://purl.obolibrary.org/obo/OBI_0003128 T cell epitope assay A T cell epitope assay that measures the binding of T cell receptor to an epitope. http://purl.obolibrary.org/obo/OBI_1110045 disposition to be bound by an adaptive immune receptor http://purl.obolibrary.org/obo/BFO_0000016 disposition Is the disposition borne by a material entity that is realized in a process of being bound by a adaptive immune receptor. http://purl.obolibrary.org/obo/OBI_1110057 MHC:epitope complex binding to TCR http://purl.obolibrary.org/obo/OBI_1110014 epitope binding by adaptive immune receptor a process of an MHC molecule binding to an entity playing the role of epitope to create an MHC:epitope complex which is then bound by a TCR molecule. http://purl.obolibrary.org/obo/OBI_1110086 restricting MHC role http://purl.obolibrary.org/obo/BFO_0000023 role The role played by an MHC molecule by binding a material entity playing the role of epitope when that epitope/MHC molecule pair are recognized (bound) by a TCR molecule on the surface of a cell playing the role of effector cell. http://purl.obolibrary.org/obo/OBI_1110093 disposition to infect an organism http://purl.obolibrary.org/obo/BFO_0000016 disposition Is a role borne by an agent, and realized when in contact with or inside another organism in which it is capable of replicating and causing disease http://purl.obolibrary.org/obo/OBI_1110108 material to be added http://purl.obolibrary.org/obo/BFO_0000040 material entity a material that is added to another one in a material combination process http://purl.obolibrary.org/obo/OBI_1110109 target of material addition http://purl.obolibrary.org/obo/BFO_0000040 material entity A material entity into which another is being added in a material combinatino process http://purl.obolibrary.org/obo/OBI_1110120 assay antigen role http://purl.obolibrary.org/obo/BFO_0000023 role Any molecule recognized by the adaptive immune receptors? Recognized means bound with a certain affinity? From GO ag binding:Interacting selectively with an antigen, any substance which is capable of inducing a specific immune response and of reacting with the products of that response, the specific antibody or specifically sensitized T-lymphocytes, or both. Binding may counteract the biological activity of the antigen. see OBI_1110120 below http://purl.obolibrary.org/obo/OBI_1110122 pathologic process http://purl.obolibrary.org/obo/GO_0008150 biological_process abnormal, harmful processes caused by or associated with a disease http://purl.obolibrary.org/obo/OBI_1110126 assay measuring binding of a MHC:ligand complex http://purl.obolibrary.org/obo/OBI_0002075 MHC ligand assay A MHC ligand assay that detects the binding of a ligand to an MHC molecule. http://purl.obolibrary.org/obo/OBI_1110127 assay measuring binding of a B cell epitope:antibody complex http://purl.obolibrary.org/obo/OBI_0003127 B cell epitope assay A B cell epitope assay that measures the binding of an antibody receptor to an epitope. http://purl.obolibrary.org/obo/OBI_1110128 immune epitope assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that detects the binding of an epitope to an adaptive immune receptor, or an immune process resulting from such a binding event, or characterizes the structrue of the complex resulting from such a binding event. http://purl.obolibrary.org/obo/OBI_9999994 chromium release assay http://purl.obolibrary.org/obo/OBI_0000903 in vitro cell killing assay An in vitro cell killing assay in which radioactive chromium is absorbed by cells and released into supernatant when the cells die. The amount of radioactivity measured in the supernatant is a proxy for the number of cells that have died. http://purl.obolibrary.org/obo/PATO_0000018 fluorescence http://purl.obolibrary.org/obo/PATO_0001296 luminous flux A luminous flux quality inhering in a bearer by virtue of the bearer's emitting longer wavelength light following the absorption of shorter wavelength radiation; fluorescence is common with aromatic compounds with several rings joined together. http://purl.obolibrary.org/obo/PATO_0000125 mass http://purl.obolibrary.org/obo/PATO_0001018 physical quality A physical quality that inheres in a bearer by virtue of the proportion of the bearer's amount of matter. http://purl.obolibrary.org/obo/PATO_0001421 alive http://purl.obolibrary.org/obo/PATO_0000169 viability A viability quality inhering in a bearer by virtue of the bearer's condition before death. http://purl.obolibrary.org/obo/PATO_0001422 dead http://purl.obolibrary.org/obo/PATO_0000169 viability A viability quality inhering in a bearer by virtue of the cessation of the bearer's life. http://purl.obolibrary.org/obo/PATO_0001741 radioactive http://purl.obolibrary.org/obo/PATO_0001740 activity (of a radionuclide) A radiation quality inhering in bearer by virtue of the bearer's exhibiting or being caused by radioactivity. http://purl.obolibrary.org/obo/PATO_0001995 organismal quality http://purl.obolibrary.org/obo/PATO_0001241 physical object quality A quality that inheres in an entire organism or part of an organism. http://purl.obolibrary.org/obo/PR_000000001 protein http://purl.obolibrary.org/obo/CHEBI_16670 peptide An amino acid chain that is produced de novo by ribosome-mediated translation of a genetically-encoded mRNA, and any derivatives thereof. http://purl.obolibrary.org/obo/PR_000023089 DNA ligase http://purl.obolibrary.org/obo/PR_000000001 protein A protein that is a translation product of the Escherichia coli K-12 ligA gene or a 1:1 ortholog thereof. http://purl.obolibrary.org/obo/PR_000025471 nuclease S1 http://purl.obolibrary.org/obo/PR_000000001 protein A protein that is a translation product of the Aspergillus oryzae RIB40 nucS gene or a 1:1 ortholog thereof. http://purl.obolibrary.org/obo/SO_0000001 region http://purl.obolibrary.org/obo/BFO_0000031 generically dependent continuant A sequence_feature with an extent greater than zero. A nucleotide region is composed of bases and a polypeptide region is composed of amino acids. http://purl.obolibrary.org/obo/UO_0000002 mass unit http://purl.obolibrary.org/obo/IAO_0000003 measurement unit label A unit which is a standard measure of the amount of matter/energy of a physical object. http://purl.obolibrary.org/obo/CHEBI_16991 deoxyribonucleic acid http://purl.obolibrary.org/obo/CHEBI_33696 nucleic acid High molecular weight, linear polymers, composed of nucleotides containing deoxyribose and linked by phosphodiester bonds; DNA contain the genetic information of organisms. http://purl.obolibrary.org/obo/CHEBI_33696 nucleic acid http://purl.obolibrary.org/obo/CHEBI_33839 macromolecule A macromolecule made up of nucleotide units and hydrolysable into certain pyrimidine or purine bases (usually adenine, cytosine, guanine, thymine, uracil), D-ribose or 2-deoxy-D-ribose and phosphoric acid. http://purl.obolibrary.org/obo/CL_0000000 cell http://purl.obolibrary.org/obo/BFO_0000040 material entity A material entity of anatomical origin (part of or deriving from an organism) that has as its parts a maximally connected cell compartment surrounded by a plasma membrane. http://purl.obolibrary.org/obo/CL_0000010 cultured cell http://purl.obolibrary.org/obo/CL_0000578 experimentally modified cell in vitro A cell in vitro that is or has been maintained or propagated as part of a cell culture. http://purl.obolibrary.org/obo/CL_0000236 B cell http://purl.obolibrary.org/obo/CL_0000542 lymphocyte A lymphocyte of B lineage that is capable of B cell mediated immunity. http://purl.obolibrary.org/obo/CL_0000540 neuron http://purl.obolibrary.org/obo/CL_0002319 neural cell The basic cellular unit of nervous tissue. Each neuron consists of a body, an axon, and dendrites. Their purpose is to receive, conduct, and transmit impulses in the nervous system. http://purl.obolibrary.org/obo/CL_0000542 lymphocyte http://purl.obolibrary.org/obo/CL_0000842 mononuclear cell A lymphocyte is a leukocyte commonly found in the blood and lymph that has the characteristics of a large nucleus, a neutral staining cytoplasm, and prominent heterochromatin. http://purl.obolibrary.org/obo/CL_0000578 experimentally modified cell in vitro http://purl.obolibrary.org/obo/CL_0000000 cell A cell in vitro that has undergone physical changes as a consequence of a deliberate and specific experimental procedure. http://purl.obolibrary.org/obo/CL_0000842 mononuclear cell http://purl.obolibrary.org/obo/CL_0000000 cell A leukocyte with a single non-segmented nucleus in the mature form. http://purl.obolibrary.org/obo/CL_0002319 neural cell http://purl.obolibrary.org/obo/CL_0000000 cell A cell that is part of the nervous system. http://purl.obolibrary.org/obo/UBERON_0000178 blood http://purl.obolibrary.org/obo/UBERON_0006314 bodily fluid A fluid that is composed of blood plasma and erythrocytes. http://purl.obolibrary.org/obo/UBERON_0000955 brain http://purl.obolibrary.org/obo/UBERON_0000465 material anatomical entity The brain is the center of the nervous system in all vertebrate, and most invertebrate, animals. Some primitive animals such as jellyfish and starfish have a decentralized nervous system without a brain, while sponges lack any nervous system at all. In vertebrates, the brain is located in the head, protected by the skull and close to the primary sensory apparatus of vision, hearing, balance, taste, and smell[WP]. http://purl.obolibrary.org/obo/ECO_0006053 pharmacological assay evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence that arises from assaying the response of a cell, tissue, organ or organism following exposure to a receptor agonist or antagonist. http://purl.obolibrary.org/obo/ECO_0006054 pharmacological assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007634 experimental phenotypic evidence used in manual assertion A type of experimental phenotypic evidence used in a manual assertion that arises from assaying the response of a cell, tissue, organ or organism following exposure to a receptor agonist or antagonist. http://purl.obolibrary.org/obo/ECO_0006055 high throughput evidence http://purl.obolibrary.org/obo/ECO_0000000 evidence A type of evidence where data generation is automated with equipment to allow for assaying samples or molecules in parallel. http://purl.obolibrary.org/obo/ECO_0006056 high throughput evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000352 evidence used in manual assertion A type of evidence that is used in a manual assertion where data generation is automated with equipment to allow for assaying large numbers of samples or molecules in parallel. http://purl.obolibrary.org/obo/ECO_0006057 high throughput evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000501 evidence used in automatic assertion A type of evidence that is used in an automatic assertion where data generation is automated with equipment to allow for assaying samples or molecules in parallel. http://purl.obolibrary.org/obo/ECO_0006058 high throughput cell biology evidence http://purl.obolibrary.org/obo/ECO_0006055 high throughput evidence A type of high throughput evidence where a classical cell biology technique is automated with equipment to allow for assaying biomolecules in parallel. http://purl.obolibrary.org/obo/ECO_0006059 high throughput cell biology evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006058 high throughput cell biology evidence A type of high throughput evidence used in a manual assertion where a classical cell biology technique is automated with equipment to allow for assaying biomolecules in parallel. http://purl.obolibrary.org/obo/ECO_0006060 high throughput cell biology evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006058 high throughput cell biology evidence A type of high throughput evidence used in an automatic assertion where a classical cell biology technique is automated with equipment to allow for assaying biomolecules in parallel. http://purl.obolibrary.org/obo/ECO_0006061 immunofluorescence wide-field microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005804 immunofluorescence evidence used in manual assertion A type of wide-field fluorescence microscopy evidence used in a manual assertion where fluorescently tagged antibodies are imaged that are used to bind to their antigens. http://purl.obolibrary.org/obo/ECO_0006062 wide-field fluorescence microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005598 wide-field fluorescence microscopy evidence A type of wide-field microscopy evidence that is used in a manual assertion where pure white and ultraviolet light are produced by a mercury lamp, passed through an optical filter (excitation filter), and directed to a sample via a dichroic mirror, followed by detection of the fluorescent light by a camera after it passes through an emission filter. http://purl.obolibrary.org/obo/ECO_0006063 over expression analysis evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000120 over expression analysis evidence A type of experimental phenotypic evidence that is used in a manual assertion where a gene and/or gene product is investigated in a transgenic organism that has been engineered to overexpress that gene product. http://purl.obolibrary.org/obo/ECO_0006064 cell-free assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of cell-free assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006066 fluorescence recovery after photobleaching evidence http://purl.obolibrary.org/obo/ECO_0001115 fluorescence evidence A type of fluorescence evidence where quantitative information on the diffusion properties of a sample is produced from the measurement of diffusion of fluorescent probes over time into an area that has been photobleached by a high-intensity laser pulse. http://purl.obolibrary.org/obo/ECO_0006067 fluorescence recovery after photobleaching evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001249 fluorescence evidence used in manual assertion A type of fluorescence evidence that is used in a manual assertion where quantitative information on the diffusion properties of a sample is produced from the measurement of diffusion of fluorescent probes over time into an area that has been photobleached by a high-intensity laser pulse. http://purl.obolibrary.org/obo/ECO_0006175 nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence http://purl.obolibrary.org/obo/ECO_0006163 nuclear magnetic resonance spectroscopy evidence A type of nuclear magnetic resonance spectroscopy evidence resulting from measuring the change in a certain type of NMR spectrum as a result of the spontenaous exchange of hydrogen and deuterium nuclei between the sample and the solvent. http://purl.obolibrary.org/obo/ECO_0006176 proton-based nuclear magnetic resonance evidence http://purl.obolibrary.org/obo/ECO_0006163 nuclear magnetic resonance spectroscopy evidence A type of nuclear magnetic resonance spectroscopy evidence in which the electromagnetic singal being measured comes from the perturbation of hydrogen nuclei resulting in a one dimensional spectrum. http://purl.obolibrary.org/obo/ECO_0006177 circular dichroism evidence http://purl.obolibrary.org/obo/ECO_0005504 spectrometry evidence A type of spectrometry evidence derived from irradiating a sample with light (either visible, infrared or ultraviolet wavelengths) and observing the difference of absorption of left-handed and right-handed light by the sample by comparing the extent of circular polarization in the incident and the emitted light. http://purl.obolibrary.org/obo/ECO_0006178 synchrotron radiation circular dichroism evidence http://purl.obolibrary.org/obo/ECO_0006177 circular dichroism evidence A type of circular dichroism evidence where the source of radiation is radially accelerated particles, most often moving on a circular path in a synchrotron, typically providing lower wavelengths and therefore higher signal-to-noise ratio compared to regular circular dichroism measurements. http://purl.obolibrary.org/obo/ECO_0006179 far-UV circular dichroism evidence http://purl.obolibrary.org/obo/ECO_0006177 circular dichroism evidence A type of circular dichroism evidence whose spectrum is in the range of 190-230 nm, providing information mainly about amide bonds and through which the relative proportion of secondary structure elements of a protein can be estimated. http://purl.obolibrary.org/obo/ECO_0006180 near-UV circular dichroism evidence http://purl.obolibrary.org/obo/ECO_0006177 circular dichroism evidence A type of circular dichroism evidence whose spectrum is in the range of 250-350 nm , providing information mainly about aromatic residues, i.e. phenylalanine, tyrosine and tryptophan, through which it provides information on the tertiary structure of a protein. http://purl.obolibrary.org/obo/ECO_0006181 cryogenic electron microscopy evidence http://purl.obolibrary.org/obo/ECO_0005033 electron microscopy evidence A type of electron microscopy evidence where the sample being studied has been cooled to cryogenic temperatures (typically below -153 degrees Celsius) prior to the experiment. http://purl.obolibrary.org/obo/ECO_0006182 small-angle X-ray scattering evidence http://purl.obolibrary.org/obo/ECO_0007061 light scattering evidence A type of light scattering evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight and structural transitions by irradiating the sample with an X-ray beam and the scattered intensity is probed at small angles (typically in the range of 0.1-10 degrees) by a detector. http://purl.obolibrary.org/obo/ECO_0006183 particle scattering evidence http://purl.obolibrary.org/obo/ECO_0005031 structure determination evidence A type of structure determination evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight, diffusion and interaction strength by irradiating the sample with a particle beam and the scattered intensity is probed at a certain angle by a detector. http://purl.obolibrary.org/obo/ECO_0006184 small-angle neutron scattering evidence http://purl.obolibrary.org/obo/ECO_0006183 particle scattering evidence A type of particle scattering evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight and structural transitions by irradiating the sample with a beam of thermal neutrons and the scattered intensity is probed at small angles (typically in the range of 0.1-10 degrees) by a detector. http://purl.obolibrary.org/obo/ECO_0006185 author inference http://purl.obolibrary.org/obo/ECO_0000361 inferential evidence A type of inferential evidence where an assertion is derived by the authors of a scientific publication from another assertion and/or primary data via logical inference or other rational means. http://purl.obolibrary.org/obo/ECO_0006186 combinatorial experimental and author inference evidence contained in single publication http://purl.obolibrary.org/obo/ECO_0007011 combinatorial experimental and author inference evidence A type of combinatorial experimental and author inference evidence where the experimental results and author inference are contained in a single publication. http://purl.obolibrary.org/obo/ECO_0006187 X-ray crystallography-based structural model with missing residue coordinates http://purl.obolibrary.org/obo/ECO_0001823 x-ray crystallography evidence A type of X-ray cystallography evidence in which a structural model is built lacking coordinates for some residues indicating high flexibility or structural disorder in that area of the molecule. http://purl.obolibrary.org/obo/ECO_0006188 X-ray crystallography-based structural model with high relative B-factor values http://purl.obolibrary.org/obo/ECO_0001823 x-ray crystallography evidence A type of X-ray cystallography evidence in which a structural model is built that contains high relative B-factor values indicating flexibility in that area of the molecule. http://purl.obolibrary.org/obo/ECO_0006189 cryogenic electron microscopy-based structural model with missing residue coordinates http://purl.obolibrary.org/obo/ECO_0006181 cryogenic electron microscopy evidence A type of cryogenic electron microscopy evidence in which a structural model is built lacking coordinates for some residues indicating high flexibility or structural disorder in that area of the molecule. http://purl.obolibrary.org/obo/ECO_0006190 cryogenic electron microscopy-based structural model with high relative B-factor values http://purl.obolibrary.org/obo/ECO_0006181 cryogenic electron microscopy evidence A type of cryogenic electron microscopy evidence in which a structural model is built that contains high relative B-factor values indicating flexibility in that area of the molecule. http://purl.obolibrary.org/obo/ECO_0006191 Fourier-transform infrared spectroscopy evidence http://purl.obolibrary.org/obo/ECO_0001126 spectrophotometry evidence A type of spectrophotometry evidence resulting from the evaluation of a molecule in a fluid or solid state by its ability to alter the transmission of infrared light, where the raw data collected is transformed into the specturm by Fourier transform. http://purl.obolibrary.org/obo/ECO_0006192 heat capacity-based evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence derived from measuring the amount of heat absorbed or released by a sample to monitor chemical reactions, phase transitions or other processes affecting the physical state or chemical composition. http://purl.obolibrary.org/obo/ECO_0006193 differential scanning calorimetry evidence http://purl.obolibrary.org/obo/ECO_0006192 heat capacity-based evidence A type of heat capacity-based evidence where the heat required to increase the temperature of the sample is continuously monitored and compared to the heat required to increase the temperature of a reference (having a largely identical composition compared to the sample, such as the empty buffer compared to a protein solution) over a range of temperatures. http://purl.obolibrary.org/obo/ECO_0006194 selective antibody-based structural conformation evidence http://purl.obolibrary.org/obo/ECO_0005593 immunodetection assay evidence A type of immunodetection assay evidence that involves the use of antibodies that are selective to conformations of the epitope to which they bind in order to provide information on conformations present in a target molecule. http://purl.obolibrary.org/obo/ECO_0006195 protein hydrogen-deuterium exchange mass spectrometry evidence http://purl.obolibrary.org/obo/ECO_0001096 mass spectrometry evidence A type of mass spectrometry evidence derived from measuring changes in mass associated with the isotopic exchange between amide hydrogens of the protein backbone and its surrounding solvent, providing information on the structural state, the dynamics and the intrinsic chemical properties of the underlying amino acid sequence. http://purl.obolibrary.org/obo/ECO_0006196 nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006165 nuclear magnetic resonance spectroscopy evidence used in manual assertion A type of nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006197 nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006164 nuclear magnetic resonance spectroscopy evidence used in automatic assertion A type of nuclear magnetic resonance spectroscopy-based hydrogen-deuterium exchange evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006198 proton-based nuclear magnetic resonance evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006165 nuclear magnetic resonance spectroscopy evidence used in manual assertion A type of proton-based nuclear magnetic resonance evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006199 proton-based nuclear magnetic resonance evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006164 nuclear magnetic resonance spectroscopy evidence used in automatic assertion A type of proton-based nuclear magnetic resonance evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006200 circular dichroism evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of circular dichroism evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006201 circular dichroism evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006177 circular dichroism evidence A type of circular dichroism evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006202 synchrotron radiation circular dichroism evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006200 circular dichroism evidence used in manual assertion A type of synchrotron radiation circular dichroism evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006203 synchrotron radiation circular dichroism evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006201 circular dichroism evidence used in automatic assertion A type of synchrotron radiation circular dichroism evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006204 far-UV circular dichroism evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006200 circular dichroism evidence used in manual assertion A type of far-UV circular dichroism evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006205 far-UV circular dichroism evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006201 circular dichroism evidence used in automatic assertion A type of far-UV circular dichroism evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006206 near-UV circular dichroism evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006200 circular dichroism evidence used in manual assertion A type of near-UV circular dichroism evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006207 near-UV circular dichroism evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006201 circular dichroism evidence used in automatic assertion A type of near-UV circular dichroism evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006208 cryogenic electron microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006003 electron microscopy evidence used in manual assertion A type of cryogenic electron microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006209 cryogenic electron microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007493 electron microscopy evidence used in automatic assertion A type of cryogenic electron microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006210 small-angle X-ray scattering evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007062 light scattering evidence used in manual assertion A type of small-angle X-ray scattering evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006211 small-angle X-ray scattering evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007617 light scattering evidence used in automatic assertion A type of small-angle X-ray scattering evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006212 particle scattering evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of particle scattering evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006213 particle scattering evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006183 particle scattering evidence A type of particle scattering evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006214 small-angle neutron scattering evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006212 particle scattering evidence used in manual assertion A type of small-angle neutron scattering evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006215 small-angle neutron scattering evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006213 particle scattering evidence used in automatic assertion A type of small-angle neutron scattering evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006216 author inference used in manual assertion http://purl.obolibrary.org/obo/ECO_0000302 author statement used in manual assertion A type of author inference that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006217 author inference used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007319 author statement used in automatic assertion A type of author inference that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006218 combinatorial experimental and author inference evidence contained in single publication used in manual assertion http://purl.obolibrary.org/obo/ECO_0007013 combinatorial experimental and author inference evidence used in manual assertion A type of combinatorial experimental and author inference evidence contained in single publication that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006219 combinatorial experimental and author inference evidence contained in single publication used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007592 combinatorial experimental and author inference evidence used in automatic assertion A type of combinatorial experimental and author inference evidence contained in single publication that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006220 X-ray crystallography-based structural model with missing residue coordinates used in manual assertion http://purl.obolibrary.org/obo/ECO_0005670 x-ray crystallography evidence used in manual assertion A type of X-ray crystallography-based structural model with missing residue coordinates that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006221 X-ray crystallography-based structural model with missing residue coordinates used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005671 x-ray crystallography evidence used in automatic assertion A type of X-ray crystallography-based structural model with missing residue coordinates that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006222 X-ray crystallography-based structural model with high relative B-factor values used in manual assertion http://purl.obolibrary.org/obo/ECO_0005670 x-ray crystallography evidence used in manual assertion A type of X-ray crystallography-based structural model with high relative B-factor values that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006223 X-ray crystallography-based structural model with high relative B-factor values used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005671 x-ray crystallography evidence used in automatic assertion A type of X-ray crystallography-based structural model with high relative B-factor values that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006224 cryogenic electron microscopy-based structural model with missing residue coordinates used in manual assertion http://purl.obolibrary.org/obo/ECO_0006208 cryogenic electron microscopy evidence used in manual assertion A type of cryogenic electron microscopy-based structural model with missing residue coordinates that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006225 cryogenic electron microscopy-based structural model with missing residue coordinates used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006209 cryogenic electron microscopy evidence used in automatic assertion A type of cryogenic electron microscopy-based structural model with missing residue coordinates that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006226 cryogenic electron microscopy-based structural model with high relative B-factor values used in manual assertion http://purl.obolibrary.org/obo/ECO_0006208 cryogenic electron microscopy evidence used in manual assertion A type of cryogenic electron microscopy-based structural model with high relative B-factor values that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006227 cryogenic electron microscopy-based structural model with high relative B-factor values used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006209 cryogenic electron microscopy evidence used in automatic assertion A type of cryogenic electron microscopy-based structural model with high relative B-factor values that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006228 Fourier-transform infrared spectroscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001258 spectrophotometry evidence used in manual assertion A type of Fourier-transform infrared spectroscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006229 Fourier-transform infrared spectroscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007458 spectrophotometry evidence used in automatic assertion A type of Fourier-transform infrared spectroscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006230 heat capacity-based evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of heat capacity-based evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006231 heat capacity-based evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of heat capacity-based evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006232 differential scanning calorimetry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006230 heat capacity-based evidence used in manual assertion A type of differential scanning calorimetry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006233 differential scanning calorimetry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006231 heat capacity-based evidence used in automatic assertion A type of differential scanning calorimetry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006234 selective antibody-based structural conformation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of selective antibody-based structural conformation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006235 selective antibody-based structural conformation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006194 selective antibody-based structural conformation evidence A type of selective antibody-based structural conformation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006236 protein hydrogen-deuterium exchange mass spectrometry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001230 mass spectrometry evidence used in manual assertion A type of protein hydrogen-deuterium exchange mass spectrometry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006237 protein hydrogen-deuterium exchange mass spectrometry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007433 mass spectrometry evidence used in automatic assertion A type of protein hydrogen-deuterium exchange mass spectrometry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006238 galactokinase reporter gene assay evidence http://purl.obolibrary.org/obo/ECO_0000049 reporter gene assay evidence A type of reporter gene assay evidence based on the fusion of the galK gene to a specific promoter for the expression of the enzyme galactokinase. http://purl.obolibrary.org/obo/ECO_0006239 polyadenylated transcript 3'-end-sequencing evidence http://purl.obolibrary.org/obo/ECO_0000295 RNA-sequencing evidence A type of RNA-sequencing evidence based on sequencing of the 3' ends of cDNA molecules that are selected by presence of a polyadenylated tail. http://purl.obolibrary.org/obo/ECO_0006240 colony boundary assay evidence http://purl.obolibrary.org/obo/ECO_0007077 colony morphology phenotypic evidence A type of colony morphology phenotypic evidence based on the presence or absence of a visually apparent boundary formed between colonies of multiple isolates or strains of the same microbial species growing on a solid surface such as that of an agar culture medium. http://purl.obolibrary.org/obo/ECO_0006241 galactokinase reporter gene assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000270 expression pattern evidence used in manual assertion A type of galactokinase reporter gene assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006242 galactokinase reporter gene assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006238 galactokinase reporter gene assay evidence A type of galactokinase reporter gene assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006243 polyadenylated transcript 3'-end-sequencing evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006068 RNA-sequencing evidence used in manual assertion A type of polyadenylated transcript 3'-end-sequencing evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006244 polyadenylated transcript 3'-end-sequencing evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006069 RNA-sequencing evidence used in automatic assertion A type of polyadenylated transcript 3'-end-sequencing evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006245 colony boundary assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007078 colony morphology phenotypic evidence used in manual assertion A type of colony boundary assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006246 colony boundary assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007625 colony morphology phenotypic evidence used in automatic assertion A type of colony boundary assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006247 analytical ultracentrifugation evidence http://purl.obolibrary.org/obo/ECO_0005516 molecule detection assay evidence A type of molecule detection assay evidence where the hydrodynamic behaviour of macromolecules is used to study their size, shape and interactions, by spinning the sample solution at high centrifugal field (up to above 100000 rpm and 1000000 g), and monitoring the evolution of sample concentration profile versus the axis of rotation. http://purl.obolibrary.org/obo/ECO_0006248 fluorescence polarization evidence http://purl.obolibrary.org/obo/ECO_0000021 physical interaction evidence A type of physical interaction evidence based on the measurement of the change in the degree of polarization of a fluorophore upon binding to a molecular partner. http://purl.obolibrary.org/obo/ECO_0006249 bait-prey protein pull-down evidence http://purl.obolibrary.org/obo/ECO_0006351 bait-prey interaction evidence A type of bait-prey interaction evidence resulting from an affinity purification in-vitro technique used to detect physical interactions between two or more proteins, where a molecule of interest - a "bait" - is immobilized and incubated with a protein source containing putative "prey" proteins. http://purl.obolibrary.org/obo/ECO_0006250 rotary shadowing electron microscopy evidence http://purl.obolibrary.org/obo/ECO_0005033 electron microscopy evidence A type of electron microscopy evidence where a heavy metal is evaporated onto surface adsorbed molecules and macromolecular assemblies allowing high-contrast visualization of both individual macromolecules and the surface structure of macromolecular assemblies. http://purl.obolibrary.org/obo/ECO_0006251 electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence http://purl.obolibrary.org/obo/ECO_0001096 mass spectrometry evidence A type of mass spectrometry evidence resulting from the ionization of polar functional groups producing multiple charged ions followed by the detection of ion cyclotron frequencies within a magnetic field, making it suitable for the study of large molecules (>2 kDa). http://purl.obolibrary.org/obo/ECO_0006252 magnetic resonance evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence in which particles in strong constant magnetic field are perturbed by a weak oscillating magnetic field and the resulting eletromagnetic signal is captured. http://purl.obolibrary.org/obo/ECO_0006253 electron paramagnetic resonance evidence http://purl.obolibrary.org/obo/ECO_0006252 magnetic resonance evidence A type of magnetic resonance evidence in which unpaired electrons in a strong constant magnetic field are perturbed by a weak oscillating magnetic field and the resulting electromagnetic signal is captured. http://purl.obolibrary.org/obo/ECO_0006254 site-directed spin-labelling electron paramagnetic resonance evidence http://purl.obolibrary.org/obo/ECO_0006253 electron paramagnetic resonance evidence A type of electron paramagnetic resonance evidence where the signal is obtained by labeling the protein in specific sites with a functional group (most often a nitroxide) containing an unpaired electron using site-directed mutagenesis. http://purl.obolibrary.org/obo/ECO_0006255 deglycosylation assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where the removal of glycan groups from a substrate (RNA, DNA, or protein) is detected. http://purl.obolibrary.org/obo/ECO_0006256 protein fragment functional complementation evidence http://purl.obolibrary.org/obo/ECO_0000021 physical interaction evidence A type of physical interaction evidence that is based on the detection of a protein-protein interaction between a bait and prey protein by reconstitution of a reporter protein serving as a detectable entity (usually an enzyme or a fluorescent protein), the two halves of which are each attached separately to the bait and prey. http://purl.obolibrary.org/obo/ECO_0006257 beta galactosidase functional complementation evidence http://purl.obolibrary.org/obo/ECO_0006256 protein fragment functional complementation evidence A type of protein fragment functional complementaion evidence where the interaction between bait and prey assemble a functional beta galactosidase enzyme. http://purl.obolibrary.org/obo/ECO_0006258 GAL4-VP16 functional complementation evidence http://purl.obolibrary.org/obo/ECO_0006256 protein fragment functional complementation evidence A type of protein fragment functional complementaion evidence where the interaction between bait and prey assemble a transcriptional activation complex consisting of the GAL4 DNA-binding domain and the transactivation domain from the herpes simplex virus protein VP16. http://purl.obolibrary.org/obo/ECO_0006259 Raman spectroscopy evidence http://purl.obolibrary.org/obo/ECO_0005031 structure determination evidence A type of spectrophotometry evidence used to determine a structural fingerprint of the molecule in solution by probing its vibrational modes via the inelastic scattering of photons (called Raman scattering). http://purl.obolibrary.org/obo/ECO_0006260 protein thermal shift assay evidence http://purl.obolibrary.org/obo/ECO_0006268 temperature-induced protein unfolding evidence A type of temperature-induced protein unfolding evidence that assess thermal denaturation temperature and protein stability under different experimental conditions, e.g. buffer pH or ionic strength, redox potential, usually by means of intrinsic fluorescence or thermofluor. http://purl.obolibrary.org/obo/ECO_0006261 microscale thermophoresis evidence http://purl.obolibrary.org/obo/ECO_0001115 fluorescence evidence A type of physical interaction evidence resulting from measuring the change of the fluorescent activity of a molecule as a function of binding to a non-fluorescent partner and its movement in a microspcopic thermal gradient affecting the intensity of fluorescence. http://purl.obolibrary.org/obo/ECO_0006262 native protein gel electrophoresis evidence http://purl.obolibrary.org/obo/ECO_0000337 gel electrophoresis evidence A type of gel electrophoresis evidence resulting from the use of non-denaturing conditions so that the proteins being measured are in their native structural states. http://purl.obolibrary.org/obo/ECO_0006264 competitive binding evidence http://purl.obolibrary.org/obo/ECO_0000023 affinity evidence A type of affinity evidence derived from an experimental setup where the binding properties of a ligand are assessed by measuring the competition with another ligand with already known binding properties. http://purl.obolibrary.org/obo/ECO_0006265 protein unfolding evidence http://purl.obolibrary.org/obo/ECO_0005031 structure determination evidence A type of structure determination evidence obtained by monitoring structural changes of a protein as a response to changes of an environmental parameter, such as pH or temperature, or the concentration of a denaturant, such as urea. http://purl.obolibrary.org/obo/ECO_0006266 urea-induced protein unfolding evidence http://purl.obolibrary.org/obo/ECO_0006265 protein unfolding evidence A type of protein unfolding evidence where the unfolding of the protein is triggered by increasing concentrations of urea. http://purl.obolibrary.org/obo/ECO_0006267 pH-induced protein unfolding evidence http://purl.obolibrary.org/obo/ECO_0006265 protein unfolding evidence A type of protein unfolding evidence where the unfolding of the protein is triggered by changes in pH. http://purl.obolibrary.org/obo/ECO_0006268 temperature-induced protein unfolding evidence http://purl.obolibrary.org/obo/ECO_0006265 protein unfolding evidence A type of protein unfolding evidence where the unfolding of the protein is triggered by changes in temperature. http://purl.obolibrary.org/obo/ECO_0006269 cell aggregation evidence http://purl.obolibrary.org/obo/ECO_0001565 cell-based assay evidence A type of cell-based assay evidence resulting from the analysis of cells irreversibly binding to each other. http://purl.obolibrary.org/obo/ECO_0006270 RNA-protein binding evidence http://purl.obolibrary.org/obo/ECO_0000136 nucleic acid binding evidence A type of affinity evidence resulting from the qualitative and quantitative analysis of the affinity with which a protein binds to RNA. http://purl.obolibrary.org/obo/ECO_0006271 fluorescence microscopy evidence http://purl.obolibrary.org/obo/ECO_0001115 fluorescence evidence A type of microscopy evidence that is obtained by using the fluorescence of the studied sample instead of or in addition to the scattering, reflection, attenuation or absorption of light. http://purl.obolibrary.org/obo/ECO_0006272 viscosity measurement evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where the viscosity of a solution is measured to quantify the material properties of the solution, or the changes of these properties in response to changing composition or environmental factors. http://purl.obolibrary.org/obo/ECO_0006273 dynamic fluorescence quenching evidence http://purl.obolibrary.org/obo/ECO_0001115 fluorescence evidence A type of physical interaction evidence derived from the decrease (quenching) of fluorescence as a result of the interaction between a fluorescence donor and acceptor in their excited state. http://purl.obolibrary.org/obo/ECO_0006274 static fluorescence quenching evidence http://purl.obolibrary.org/obo/ECO_0001115 fluorescence evidence A type of physical interaction evidence derived from the decrease (quenching) of fluorescence as a result of the intramolecular interaction between a fluorescence donor and acceptor creating a non-fluorescent ground state. http://purl.obolibrary.org/obo/ECO_0006275 analytical ultracentrifugation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of analytical ultracentrifugation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006276 analytical ultracentrifugation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006247 analytical ultracentrifugation evidence A type of analytical ultracentrifugation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006277 fluorescence polarization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000353 physical interaction evidence used in manual assertion A type of fluorescence polarization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006278 fluorescence polarization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007332 physical interaction evidence used in automatic assertion A type of fluorescence polarization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006279 bait-prey protein pull-down evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006392 bait-prey interaction evidence used in manual assertion A type of bait-prey protein pull-down evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006280 bait-prey protein pull-down evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006393 bait-prey interaction evidence used in automatic assertion A type of bait-prey protein pull-down evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006281 rotary shadowing electron microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006003 electron microscopy evidence used in manual assertion A type of rotary shadowing electron microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006282 rotary shadowing electron microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007493 electron microscopy evidence used in automatic assertion A type of rotary shadowing electron microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006283 electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001230 mass spectrometry evidence used in manual assertion A type of electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006284 electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007433 mass spectrometry evidence used in automatic assertion A type of electrospray ionization fourier transform ion cyclotron resonance mass spectrometry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006285 magnetic resonance evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of magnetic resonance evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006286 magnetic resonance evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of magnetic resonance evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006287 electron paramagnetic resonance evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006285 magnetic resonance evidence used in manual assertion A type of electron paramagnetic resonance evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006288 electron paramagnetic resonance evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006286 magnetic resonance evidence used in automatic assertion A type of electron paramagnetic resonance evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006289 site-directed spin-labelling electron paramagnetic resonance evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006287 electron paramagnetic resonance evidence used in manual assertion A type of site-directed spin-labelling electron paramagnetic resonance evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006290 site-directed spin-labelling electron paramagnetic resonance evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006288 electron paramagnetic resonance evidence used in automatic assertion A type of site-directed spin-labelling electron paramagnetic resonance evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006291 deglycosylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of deglycosylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006292 deglycosylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of deglycosylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006293 protein fragment functional complementation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000353 physical interaction evidence used in manual assertion A type of protein fragment functional complementation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006294 protein fragment functional complementation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007332 physical interaction evidence used in automatic assertion A type of protein fragment functional complementation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006295 beta galactosidase functional complementation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006293 protein fragment functional complementation evidence used in manual assertion A type of beta galactosidase functional complementation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006296 beta galactosidase functional complementation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006294 protein fragment functional complementation evidence used in automatic assertion A type of beta galactosidase functional complementation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006297 GAL4-VP16 functional complementation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006293 protein fragment functional complementation evidence used in manual assertion A type of GAL4-VP16 functional complementation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006298 GAL4-VP16 functional complementation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006294 protein fragment functional complementation evidence used in automatic assertion A type of GAL4-VP16 functional complementation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006299 Raman spectroscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001258 spectrophotometry evidence used in manual assertion A type of Raman spectroscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006300 Raman spectroscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007458 spectrophotometry evidence used in automatic assertion A type of Raman spectroscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006301 protein thermal shift assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006317 temperature-induced protein unfolding evidence used in manual assertion A type of protein thermal shift assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006302 protein thermal shift assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006318 temperature-induced protein unfolding evidence used in automatic assertion A type of protein thermal shift assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006304 microscale thermophoresis evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007450 fluorescence evidence used in automatic assertion A type of microscale thermophoresis evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006305 native protein gel electrophoresis evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of native protein gel electrophoresis evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006306 native protein gel electrophoresis evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006262 native protein gel electrophoresis evidence A type of native protein gel electrophoresis evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006307 turbidity measurement evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006263 turbidity measurement evidence A type of turbidity measurement evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006308 turbidity measurement evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006263 turbidity measurement evidence A type of turbidity measurement evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006309 competitive binding evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006075 affinity evidence used in manual assertion A type of competitive binding evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006310 competitive binding evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007384 affinity evidence used in automatic assertion A type of competitive binding evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006311 protein unfolding evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of protein unfolding evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006312 protein unfolding evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006265 protein unfolding evidence A type of protein unfolding evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006313 urea-induced protein unfolding evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006311 protein unfolding evidence used in manual assertion A type of urea-induced protein unfolding evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006314 urea-induced protein unfolding evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006312 protein unfolding evidence used in automatic assertion A type of urea-induced protein unfolding evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006315 pH-induced protein unfolding evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006311 protein unfolding evidence used in manual assertion A type of pH-induced protein unfolding evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006316 pH-induced protein unfolding evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006312 protein unfolding evidence used in automatic assertion A type of pH-induced protein unfolding evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006317 temperature-induced protein unfolding evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006311 protein unfolding evidence used in manual assertion A type of temperature-induced protein unfolding evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006318 temperature-induced protein unfolding evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006312 protein unfolding evidence used in automatic assertion A type of temperature-induced protein unfolding evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006319 cell aggregation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006269 cell aggregation evidence A type of cell aggregation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006320 cell aggregation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006269 cell aggregation evidence A type of cell aggregation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006321 RNA-protein binding evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006083 nucleic acid binding evidence used in manual assertion A type of RNA-protein binding evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006322 RNA-protein binding evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007505 nucleic acid binding evidence used in automatic assertion A type of RNA-protein binding evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006323 fluorescence microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001249 fluorescence evidence used in manual assertion A type of fluorescence microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006324 fluorescence microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007450 fluorescence evidence used in automatic assertion A type of fluorescence microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006325 viscosity measurement evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of viscosity measurement evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006326 viscosity measurement evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of viscosity measurement evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006327 dynamic fluorescence quenching evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001249 fluorescence evidence used in manual assertion A type of dynamic fluorescence quenching evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006328 dynamic fluorescence quenching evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007450 fluorescence evidence used in automatic assertion A type of dynamic fluorescence quenching evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006329 static fluorescence quenching evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001249 fluorescence evidence used in manual assertion A type of static fluorescence quenching evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006330 static fluorescence quenching evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007450 fluorescence evidence used in automatic assertion A type of static fluorescence quenching evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006404 experimentally evolved mutant phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence where mutations arising in organisms/cells over multiple generations, with or without exposure to some kind of selective pressure, are monitored or assayed. http://purl.obolibrary.org/obo/ECO_0006405 experimentally evolved mutant phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of experimentally evolved mutant phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006406 experimentally evolved mutant phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000315 mutant phenotype evidence used in manual assertion A type of experimentally evolved mutant phenotyic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/CHEBI_16670 peptide http://purl.obolibrary.org/obo/CHEBI_33839 macromolecule Amide derived from two or more amino carboxylic acid molecules (the same or different) by formation of a covalent bond from the carbonyl carbon of one to the nitrogen atom of another with formal loss of water. The term is usually applied to structures formed from alpha-amino acids, but it includes those derived from any amino carboxylic acid. X = OH, OR, NH2, NHR, etc. http://purl.obolibrary.org/obo/CHEBI_33839 macromolecule http://purl.obolibrary.org/obo/CHEBI_23367 molecular entity A macromolecule is a molecule of high relative molecular mass, the structure of which essentially comprises the multiple repetition of units derived, actually or conceptually, from molecules of low relative molecular mass. http://purl.obolibrary.org/obo/CHEBI_33697 ribonucleic acid http://purl.obolibrary.org/obo/CHEBI_33696 nucleic acid High molecular weight, linear polymers, composed of nucleotides containing ribose and linked by phosphodiester bonds; RNA is central to the synthesis of proteins. http://purl.obolibrary.org/obo/CHEBI_472552 5-bromo-2'-deoxyuridine http://purl.obolibrary.org/obo/CHEBI_23367 molecular entity A pyrimidine 2'-deoxyribonucleoside compound having 5-bromouracil as the nucleobase. http://purl.obolibrary.org/obo/GO_0005216 ion channel activity http://purl.obolibrary.org/obo/GO_0015318 inorganic molecular entity transmembrane transporter activity Enables the facilitated diffusion of an ion (by an energy-independent process) by passage through a transmembrane aqueous pore or channel without evidence for a carrier-mediated mechanism. May be either selective (it enables passage of a specific ion only) or non-selective (it enables passage of two or more ions of same charge but different size). http://purl.obolibrary.org/obo/GO_0005575 cellular_component http://purl.obolibrary.org/obo/BFO_0000040 material entity A location, relative to cellular compartments and structures, occupied by a macromolecular machine when it carries out a molecular function. There are two ways in which the gene ontology describes locations of gene products: (1) relative to cellular structures (e.g., cytoplasmic side of plasma membrane) or compartments (e.g., mitochondrion), and (2) the stable macromolecular complexes of which they are parts (e.g., the ribosome). http://purl.obolibrary.org/obo/GO_0006306 DNA methylation http://purl.obolibrary.org/obo/GO_0043414 macromolecule methylation The covalent transfer of a methyl group to either N-6 of adenine or C-5 or N-4 of cytosine. http://purl.obolibrary.org/obo/GO_0033036 macromolecule localization http://purl.obolibrary.org/obo/GO_0051179 localization Any process in which a macromolecule is transported to, or maintained in, a specific location. http://purl.obolibrary.org/obo/GO_0050896 response to stimulus http://purl.obolibrary.org/obo/GO_0008150 biological_process Any process that results in a change in state or activity of a cell or an organism (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus. The process begins with detection of the stimulus and ends with a change in state or activity or the cell or organism. http://purl.obolibrary.org/obo/UBERON_0000463 organism substance http://purl.obolibrary.org/obo/UBERON_0000465 material anatomical entity Material anatomical entity in a gaseous, liquid, semisolid or solid state; produced by anatomical structures or derived from inhaled and ingested substances that have been modified by anatomical structures as they pass through the body. http://purl.obolibrary.org/obo/UBERON_0000465 material anatomical entity http://purl.obolibrary.org/obo/BFO_0000040 material entity Anatomical entity that has mass. http://purl.obolibrary.org/obo/UBERON_0000477 anatomical cluster http://purl.obolibrary.org/obo/BFO_0000040 material entity Anatomical group that has its parts adjacent to one another. http://purl.obolibrary.org/obo/UBERON_0000479 tissue http://purl.obolibrary.org/obo/UBERON_0000465 material anatomical entity Multicellular anatomical structure that consists of many cells of one or a few types, arranged in an extracellular matrix such that their long-range organisation is at least partly a repetition of their short-range organisation. http://purl.obolibrary.org/obo/UBERON_0001088 urine http://purl.obolibrary.org/obo/UBERON_0000463 organism substance Excretion that is the output of a kidney http://purl.obolibrary.org/obo/UBERON_0006314 bodily fluid http://purl.obolibrary.org/obo/UBERON_0000463 organism substance Liquid components of living organisms. includes fluids that are excreted or secreted from the body as well as body water that normally is not. http://purl.obolibrary.org/obo/ECO_0000353 physical interaction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of physical interaction evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006042 cell fractionation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006005 fractionation evidence used in manual assertion A type of cell fractionation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006043 extracellular recording evidence http://purl.obolibrary.org/obo/ECO_0000164 electrophysiology assay evidence A type of electrophysiology assay evidence resulting from the use of electrodes to measure in vivo electrical activity coming from adjacent neurons. http://purl.obolibrary.org/obo/ECO_0006044 extracellular recording evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006006 electrophysiology assay evidence used in manual assertion A type of extracellular recording evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006045 single-unit extracellular recording evidence http://purl.obolibrary.org/obo/ECO_0006043 extracellular recording evidence A type of extracellular recording evidence in which an extracellular microelectrode is used to measure the electrical activity of a single neuron. http://purl.obolibrary.org/obo/ECO_0006046 single-unit extracellular recording evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006045 single-unit extracellular recording evidence A type of single-unit extracellular recording evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006047 field potential recording evidence http://purl.obolibrary.org/obo/ECO_0006043 extracellular recording evidence A type of extracellular recording evidence in which electrical activity is measured in either tissue or at a cellular level with microelectrodes. http://purl.obolibrary.org/obo/ECO_0006048 field potential recording evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006047 field potential recording evidence A type of field potential recording evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006049 genetic transformation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005027 genetic transformation evidence A type of genetic transformation evidence that is used in a manual assertion http://purl.obolibrary.org/obo/ECO_0006050 anti-sense experiment evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000315 mutant phenotype evidence used in manual assertion A type of anti-sense experiment evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006051 morpholino experiment evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000315 mutant phenotype evidence used in manual assertion A type of morpholino experiment evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006052 RNAi evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000315 mutant phenotype evidence used in manual assertion A type of RNAi evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006395 self-reported ancestry evidence http://purl.obolibrary.org/obo/ECO_0006153 self-reported individual's statement evidence A type of self-reported individual's statement evidence in which a person reports their ancestry and that information is taken at face value. http://purl.obolibrary.org/obo/ECO_0006396 self-reported ancestry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006395 self-reported ancestry evidence A type of self-reported ancestry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006397 self-reported ancestry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006395 self-reported ancestry evidence A type of self-reported ancestry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/CHEBI_15422 ATP http://purl.obolibrary.org/obo/CHEBI_23367 molecular entity An adenosine 5'-phosphate in which the 5'-phosphate is a triphosphate group. It is involved in the transportation of chemical energy during metabolic pathways. http://purl.obolibrary.org/obo/CHEBI_53526 tritiated thymidine http://purl.obolibrary.org/obo/CHEBI_23367 molecular entity Thymidine linked to the radioisotope tritium. Used to label DNA in the study of cellular and viral DNA synthesis. http://purl.obolibrary.org/obo/ECO_0007011 combinatorial experimental and author inference evidence http://purl.obolibrary.org/obo/ECO_0000212 combinatorial evidence A type of combinatorial evidence in which the author draws a conclusion based on a combination of prior scientific background knowledge and evidence from one or more experiments. http://purl.obolibrary.org/obo/ECO_0007012 combinatorial experimental and curator inference evidence http://purl.obolibrary.org/obo/ECO_0000212 combinatorial evidence A type of combinatorial evidence in which the curator draws a conclusion based on a combination of prior scientific background knowledge and evidence from one or more experiments. http://purl.obolibrary.org/obo/ECO_0007013 combinatorial experimental and author inference evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000244 combinatorial evidence used in manual assertion A type of combinatorial evidence from author knowledge and experimental evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007014 combinatorial experimental and curator inference evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000244 combinatorial evidence used in manual assertion A type of combinatorial evidence from curator knowledge and experimental evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007015 voltammetry evidence http://purl.obolibrary.org/obo/ECO_0000335 substance quantification evidence A type of substance quantification evidence where an electrical gradient potential is applied to analyze an analyte of interest, resulting in a measurement amount of electrical current across the potential range applied. http://purl.obolibrary.org/obo/ECO_0007016 voltammetry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of voltammetry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007017 photoconversion evidence http://purl.obolibrary.org/obo/ECO_0001115 fluorescence evidence A type of fluorescence evidence where a molecule tagged with a fluorescent protein is exposed to ultraviolet or blue light to shift the spectral emission properties for visualization. http://purl.obolibrary.org/obo/ECO_0007018 photoconversion evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001249 fluorescence evidence used in manual assertion A type of photoconversion evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/COB_0000080 complex of molecular entities http://purl.obolibrary.org/obo/BFO_0000040 material entity A complex of two or more molecular entities that are not covalently bound. http://purl.obolibrary.org/obo/ECO_0006172 mixed support of intron positions by RNA-sequencing alignment evidence http://purl.obolibrary.org/obo/ECO_0000342 support of intron positions by RNA-sequencing alignment evidence A type of support of intron positions by RNA-sequencing alignment evidence where RNA-seq alignments from a mixture of samples support all of the intron positions (exon pairs) predicted for a transcript. http://purl.obolibrary.org/obo/ECO_0006173 mixed support of intron positions by RNA-sequencing alignment evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000347 support of intron positions by RNA-sequencing alignment evidence used in automatic assertion A type of mixed support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006174 mixed support of intron positions by RNA-sequencing alignment evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000346 support of intron positions by RNA-sequencing alignment evidence used in manual assertion A type of mixed support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion http://purl.obolibrary.org/obo/ECO_0007087 ribosome profiling evidence http://purl.obolibrary.org/obo/ECO_0000008 expression pattern evidence A type of expression pattern evidence in which translation is measured by the positions of ribosomes active in a cell, identified through deep sequencing of ribosome-protected mRNA fragments. http://purl.obolibrary.org/obo/ECO_0001560 single-cell RNA-sequencing evidence http://purl.obolibrary.org/obo/ECO_0000295 RNA-sequencing evidence A type of RNA-sequencing evidence that uses a single cell as the source of the RNA. http://purl.obolibrary.org/obo/ECO_000157 colony diameter phenotype evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007082 colony size phenotypic evidence used in manual assertion A type of colony diameter phenotype evidence that is used in manual assertion. http://purl.obolibrary.org/obo/ECO_0001570 single-cell RNA-sequencing evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006068 RNA-sequencing evidence used in manual assertion A type of single cell RNA-sequencing evidence that is used in manual assertion. http://purl.obolibrary.org/obo/ECO_0001571 colony diameter phenotype evidence http://purl.obolibrary.org/obo/ECO_0007081 colony size phenotypic evidence A type of colony size phenotypic evidence resulting from measurement of a microbial colony's diameter. http://purl.obolibrary.org/obo/ECO_0001572 colony diameter phenotype evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007627 colony size phenotypic evidence used in automatic assertion A type of colony diameter phenotype evidence that is used in automatic assertion http://purl.obolibrary.org/obo/OBI_0003145 assay measuring quantitative binding of a MHC:ligand complex http://purl.obolibrary.org/obo/OBI_1110126 assay measuring binding of a MHC:ligand complex An assay that detects the binding of a MHC molecule with a ligand, and produces a quantitative measurement of the binding as an output. http://purl.obolibrary.org/obo/OBI_0003146 assay measuring quantitative binding of an antigen:antibody complex http://purl.obolibrary.org/obo/OBI_1110127 assay measuring binding of a B cell epitope:antibody complex An assay that detects the binding of an antigen with an antibody, and produces a quantitative measurement of the binding as an output. http://purl.obolibrary.org/obo/ECO_0006407 single-cell RNA-sequencing evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006069 RNA-sequencing evidence used in automatic assertion A type of single cell RNA-sequencing evidence that is used in automatic assertion. http://purl.obolibrary.org/obo/GO_0000785 chromatin http://purl.obolibrary.org/obo/GO_0005575 cellular_component The ordered and organized complex of DNA, protein, and sometimes RNA, that forms the chromosome. http://purl.obolibrary.org/obo/GO_0001046 core promoter sequence-specific DNA binding http://purl.obolibrary.org/obo/GO_0000976 transcription cis-regulatory region binding Interacting selectively and non-covalently with a sequence of DNA that is part of a core promoter region. The core promoter is composed of the transcription start site and binding sites for the RNA polymerase and the basal transcription machinery. The transcribed region might be described as a gene, cistron, or operon. http://purl.obolibrary.org/obo/GO_0001172 transcription, RNA-templated http://purl.obolibrary.org/obo/GO_0009987 cellular process The cellular synthesis of RNA on a template of RNA. http://purl.obolibrary.org/obo/GO_0001508 action potential http://purl.obolibrary.org/obo/GO_0042391 regulation of membrane potential A process in which membrane potential cycles through a depolarizing spike, triggered in response to depolarization above some threshold, followed by repolarization. This cycle is driven by the flow of ions through various voltage gated channels with different thresholds and ion specificities. http://purl.obolibrary.org/obo/GO_0001906 cell killing http://purl.obolibrary.org/obo/GO_0009987 cellular process Any process in an organism that results in the killing of its own cells or those of another organism, including in some cases the death of the other organism. Killing here refers to the induction of death in one cell by another cell, not cell-autonomous death due to internal or other environmental conditions. http://purl.obolibrary.org/obo/GO_0003674 molecular_function http://purl.obolibrary.org/obo/BFO_0000015 process A molecular process that can be carried out by the action of a single macromolecular machine, usually via direct physical interactions with other molecular entities. Function in this sense denotes an action, or activity, that a gene product (or a complex) performs. These actions are described from two distinct but related perspectives: (1) biochemical activity, and (2) role as a component in a larger system/process. http://purl.obolibrary.org/obo/GO_0003824 catalytic activity http://purl.obolibrary.org/obo/GO_0003674 molecular_function Catalysis of a biochemical reaction at physiological temperatures. In biologically catalyzed reactions, the reactants are known as substrates, and the catalysts are naturally occurring macromolecular substances known as enzymes. Enzymes possess specific binding sites for substrates, and are usually composed wholly or largely of protein, but RNA that has catalytic activity (ribozyme) is often also regarded as enzymatic. http://purl.obolibrary.org/obo/GO_0003964 RNA-directed DNA polymerase activity http://purl.obolibrary.org/obo/GO_0003824 catalytic activity Catalysis of the reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). Catalyzes RNA-template-directed extension of the 3'- end of a DNA strand by one deoxynucleotide at a time. http://purl.obolibrary.org/obo/GO_0005694 chromosome http://purl.obolibrary.org/obo/GO_0005575 cellular_component A structure composed of a very long molecule of DNA and associated proteins (e.g. histones) that carries hereditary information. http://purl.obolibrary.org/obo/GO_0006974 cellular response to DNA damage stimulus http://purl.obolibrary.org/obo/GO_0033554 cellular response to stress Any process that results in a change in state or activity of a cell (in terms of movement, secretion, enzyme production, gene expression, etc.) as a result of a stimulus indicating damage to its DNA from environmental insults or errors during metabolism. http://purl.obolibrary.org/obo/GO_0008150 biological_process http://purl.obolibrary.org/obo/BFO_0000015 process A biological process represents a specific objective that the organism is genetically programmed to achieve. Biological processes are often described by their outcome or ending state, e.g., the biological process of cell division results in the creation of two daughter cells (a divided cell) from a single parent cell. A biological process is accomplished by a particular set of molecular functions carried out by specific gene products (or macromolecular complexes), often in a highly regulated manner and in a particular temporal sequence. http://purl.obolibrary.org/obo/GO_0008283 cell population proliferation http://purl.obolibrary.org/obo/GO_0009987 cellular process The multiplication or reproduction of cells, resulting in the expansion of a cell population. http://purl.obolibrary.org/obo/GO_0009987 cellular process http://purl.obolibrary.org/obo/GO_0008150 biological_process Any process that is carried out at the cellular level, but not necessarily restricted to a single cell. For example, cell communication occurs among more than one cell, but occurs at the cellular level. http://purl.obolibrary.org/obo/GO_0010467 gene expression http://purl.obolibrary.org/obo/GO_0043170 macromolecule metabolic process The process in which a gene's sequence is converted into a mature gene product or products (proteins or RNA). This includes the production of an RNA transcript as well as any processing to produce a mature RNA product or an mRNA or circRNA (for protein-coding genes) and the translation of that mRNA or circRNA into protein. Protein maturation is included when required to form an active form of a product from an inactive precursor form. http://purl.obolibrary.org/obo/GO_0030097 hemopoiesis http://purl.obolibrary.org/obo/GO_0008150 biological_process The process whose specific outcome is the progression of the myeloid and lymphoid derived organ/tissue systems of the blood and other parts of the body over time, from formation to the mature structure. The site of hemopoiesis is variable during development, but occurs primarily in bone marrow or kidney in many adult vertebrates. http://purl.obolibrary.org/obo/GO_0032991 protein-containing complex http://purl.obolibrary.org/obo/GO_0005575 cellular_component A stable assembly of two or more macromolecules, i.e. proteins, nucleic acids, carbohydrates or lipids, in which at least one component is a protein and the constituent parts function together. http://purl.obolibrary.org/obo/GO_0034061 DNA polymerase activity http://purl.obolibrary.org/obo/GO_0003824 catalytic activity Catalysis of the reaction: deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1); the synthesis of DNA from deoxyribonucleotide triphosphates in the presence of a nucleic acid template and a 3'hydroxyl group. http://purl.obolibrary.org/obo/GO_0042575 DNA polymerase complex http://purl.obolibrary.org/obo/GO_0032991 protein-containing complex A protein complex that possesses DNA polymerase activity and is involved in template directed synthesis of DNA. http://purl.obolibrary.org/obo/GO_0051276 chromosome organization http://purl.obolibrary.org/obo/GO_0006996 organelle organization A process that is carried out at the cellular level that results in the assembly, arrangement of constituent parts, or disassembly of chromosomes, structures composed of a very long molecule of DNA and associated proteins that carries hereditary information. This term covers covalent modifications at the molecular level as well as spatial relationships among the major components of a chromosome. http://purl.obolibrary.org/obo/GO_0019814 immunoglobulin complex http://purl.obolibrary.org/obo/GO_0032991 protein-containing complex A protein complex that in its canonical form is composed of two identical immunoglobulin heavy chains and two identical immunoglobulin light chains, held together by disulfide bonds and sometimes complexed with additional proteins. An immunoglobulin complex may be embedded in the plasma membrane or present in the extracellular space, in mucosal areas or other tissues, or circulating in the blood or lymph. http://purl.obolibrary.org/obo/GO_0019815 B cell receptor complex http://purl.obolibrary.org/obo/OBI_1110022 adaptive immune receptor An immunoglobulin complex that is present in the plasma membrane of B cells and that in its canonical form is composed of two identical immunoglobulin heavy chains and two identical immunoglobulin light chains and a signaling subunit, a heterodimer of the Ig-alpha and Ig-beta proteins. http://purl.obolibrary.org/obo/GO_0030041 actin filament polymerization http://purl.obolibrary.org/obo/GO_0008150 biological_process Assembly of actin filaments by the addition of actin monomers to a filament. http://purl.obolibrary.org/obo/GO_0042611 MHC protein complex http://purl.obolibrary.org/obo/GO_0032991 protein-containing complex A transmembrane protein complex composed of an MHC alpha chain and, in most cases, either an MHC class II beta chain or an invariant beta2-microglobin chain, and with or without a bound peptide, lipid, or polysaccharide antigen. http://purl.obolibrary.org/obo/GO_0040030 regulation of molecular function, epigenetic http://purl.obolibrary.org/obo/GO_0008150 biological_process Any heritable epigenetic process that modulates the frequency, rate or extent of protein function by self-perpetuating conformational conversions of normal proteins in healthy cells. This is distinct from, though mechanistically analogous to, disease states associated with prion propagation and amyloidogenesis. A single protein, if it carries a glutamine/asparagine-rich ('prion') domain, can sometimes stably exist in at least two distinct physical states, each associated with a different phenotype; propagation of one of these traits is achieved by a self-perpetuating change in the protein from one form to the other, mediated by conformational changes in the glutamine/asparagine-rich domain. Prion domains are both modular and transferable to other proteins, on which they can confer a heritable epigenetic alteration of function; existing bioinformatics data indicate that they are rare in non-eukarya, but common in eukarya. http://purl.obolibrary.org/obo/GO_0042101 T cell receptor complex http://purl.obolibrary.org/obo/OBI_1110022 adaptive immune receptor A protein complex that contains a disulfide-linked heterodimer of T cell receptor (TCR) chains, which are members of the immunoglobulin superfamily, and mediates antigen recognition, ultimately resulting in T cell activation. The TCR heterodimer is associated with the CD3 complex, which consists of the nonpolymorphic polypeptides gamma, delta, epsilon, zeta, and, in some cases, eta (an RNA splice variant of zeta) or Fc epsilon chains. http://purl.obolibrary.org/obo/GO_0042571 immunoglobulin complex, circulating http://purl.obolibrary.org/obo/GO_0019814 immunoglobulin complex An immunoglobulin complex that is secreted into extracellular space and found in mucosal areas or other tissues or circulating in the blood or lymph. In its canonical form, a circulating immunoglobulin complex is composed of two identical heavy chains and two identical light chains, held together by disulfide bonds. Some forms of are polymers of the basic structure and contain additional components such as J-chain and the secretory component. http://purl.obolibrary.org/obo/GO_0044030 regulation of DNA methylation http://purl.obolibrary.org/obo/GO_0040030 regulation of molecular function, epigenetic Any process that modulates the frequency, rate or extent of the covalent transfer of a methyl group to either N-6 of adenine or C-5 or N-4 of cytosine. http://purl.obolibrary.org/obo/ECO_0006068 RNA-sequencing evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000295 RNA-sequencing evidence A type of high throughput nucleotide sequencing assay evidence used in a manual assertion based on high-throughput (HT) sequencing of fragmented cDNA molecules. http://purl.obolibrary.org/obo/ECO_0006069 RNA-sequencing evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007303 transcript expression evidence used in automatic assertion A type of high throughput nucleotide sequencing assay evidence used in an automatic assertion based on high-throughput (HT) sequencing of fragmented cDNA molecules. http://purl.obolibrary.org/obo/ECO_0006070 immuno-labelling electron microscopy evidence http://purl.obolibrary.org/obo/ECO_0005033 electron microscopy evidence A type of electron microscopy evidence resulting from the use of antibodies to identify the localization of antigens in cells and tissues. http://purl.obolibrary.org/obo/ECO_0006071 immuno-labelling electron microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006003 electron microscopy evidence used in manual assertion A type of electron microscopy evidence used in a manual assertion resulting from the use of antibodies to identify the localization of antigens in cells and tissues. http://purl.obolibrary.org/obo/ECO_0006072 immunofluorescence super resolution microscopy evidence http://purl.obolibrary.org/obo/ECO_0005578 super-resolution microscopy evidence A type of super-resolution microscopy evidence resulting from the use of fluorescently-tagged antibodies to identify the localization of antigens in cells and tissue. http://purl.obolibrary.org/obo/ECO_0006073 immunofluorescence super resolution microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006004 super-resolution microscopy evidence used in manual assertion A type of super-resolution microscopy evidence used in a manual assertion resulting from the use of fluorescently-tagged antibodies to identify the localization of antigens in cells and tissue. http://purl.obolibrary.org/obo/ECO_0006074 co-purification evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000022 co-purification evidence A type of physical interaction evidence that is used in manual assertion where a cellular component subunit is isolated as part of purification of its larger complex. http://purl.obolibrary.org/obo/ECO_0006075 affinity evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000023 affinity evidence A type of physical interaction evidence that is used in manual assertion that depends on the strength of the interaction between two entities. http://purl.obolibrary.org/obo/ECO_0006076 protein binding evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000024 protein binding evidence A type of affinity evidence that is used in manual assertion resulting from the binding of a molecule to a protein or protein complex. http://purl.obolibrary.org/obo/ECO_0006077 bait-prey hybrid interaction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006392 bait-prey interaction evidence used in manual assertion A type of bait-prey hybrid interaction evidence that is used in manual assertion where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein. http://purl.obolibrary.org/obo/ECO_0006078 immunological assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000040 immunological assay evidence A type of affinity evidence that is used in manual assertion resulting from quantitation of the analyte which depends on the reaction of an antigen (analyte) and an antibody. http://purl.obolibrary.org/obo/ECO_0006079 yeast one-hybrid evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000066 yeast one-hybrid evidence A type of hybrid interaction evidence that is used in manual assertion that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors. http://purl.obolibrary.org/obo/ECO_0006080 split-ubiquitin functional complementation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000074 split-ubiquitin functional complementation evidence A type of split-ubiquitin functional complementation evidence that is used in a manual assertion that is based on detection of protein-protein interaction between a bait and prey protein by in vivo reconstitution of split-ubiquitin (when bait and prey interact) and release of a reporter protein. http://purl.obolibrary.org/obo/ECO_0006081 far-Western blotting evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000076 far-Western blotting evidence A type of physical interaction evidence that is used in a manual assertion that is based on detection of protein-protein interactions by separation of target proteins by SDS-PAGE which are blotted to a membrane, followed by denaturation and renaturation, probing with purified bait proteins, and detection of the target-bait complexes. http://purl.obolibrary.org/obo/ECO_0006082 affinity chromatography evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000079 affinity chromatography evidence A type of affinity evidence that is used in a manual assertion that results from separation of biochemical mixtures by selective binding of a compound to an immobilized compound on a polymeric matrix, subsequent removal of unattached components, and then displacement of the bond compound. http://purl.obolibrary.org/obo/ECO_0006083 nucleic acid binding evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000136 nucleic acid binding evidence A type of affinity evidence that is used in a manual assertion resulting from the binding of a molecule to a nucleic acid. http://purl.obolibrary.org/obo/ECO_0006084 ribohomopolymer binding assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000138 ribohomopolymer binding assay evidence A type of nucleic acid binding evidence that is used in a manual assertion resulting from an enzyme displaying binding activity to specific ribohomopolymer. http://purl.obolibrary.org/obo/ECO_0006085 protein:ion binding evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000142 protein:ion binding evidence A type of protein binding evidence that is used in a manual assertion resulting from a metal ion binding to a protein at a specific binding site. http://purl.obolibrary.org/obo/ECO_0006086 Southwestern blot evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000144 Southwestern blot evidence A type of nucleic acid binding evidence that is used in a manual assertion in which DNA-protein binding is detected using labeled DNA as probes, hybridized to electrophoretically separated proteins. http://purl.obolibrary.org/obo/ECO_0006087 Northwestern blot evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000146 Northwestern blot evidence A type of nucleic acid binding evidence that is used in a manual assertion in which RNA-protein binding is detected using labeled RNA as probes, hybridized to electrophoretically separated proteins. http://purl.obolibrary.org/obo/ECO_0006088 systematic evolution of ligands by exponential amplification evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000293 systematic evolution of ligands by exponential amplification evidence A type of evidence that is used in a manual assertion arising from a physical interaction analysis where a combinatorial chemistry technique is used to identify oligonucleotides that bind to a target ligand. http://purl.obolibrary.org/obo/ECO_0006089 bacterial one-hybrid evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000294 bacterial one-hybrid evidence A type of hybrid interaction evidence that is used in a manual assertion that uses bacterial transformation with two plasmids to assess in vivo binding of a DNA-binding domain (bait) and DNA target site (prey). http://purl.obolibrary.org/obo/ECO_0006090 protein-oligonucleotide microarray binding evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001601 protein-oligonucleotide microarray binding evidence A type of protein-oligonucleotide microarray binding evidence that is used in a manual assertion that detects binding of a tagged protein to an array of oligonucleotide probes representing potential binding sites. http://purl.obolibrary.org/obo/ECO_0006091 functional complementation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000316 genetic interaction evidence used in manual assertion A type of genetic interaction evidence that is used in a manual assertion where a wild-type copy of the gene in question is inserted into a mutant cell to see if it restores the wild-type phenotype in the mutant background. http://purl.obolibrary.org/obo/ECO_0006092 transgenic rescue experiment evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000013 transgenic rescue experiment evidence A type of functional complementation evidence that is used in a manual assertion that is used in manual assertion resulting from the introduction of a transgene to prevent, or "rescue" an organism from a condition. http://purl.obolibrary.org/obo/ECO_0006093 transient rescue experiment evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000038 transient rescue experiment evidence A type of transient rescue experiment evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006094 suppressor/enhancer interaction phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000316 genetic interaction evidence used in manual assertion A type of suppressor/enhancer interaction phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006095 double mutant phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000315 mutant phenotype evidence used in manual assertion A type of double mutant phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006096 epistatic interaction phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000316 genetic interaction evidence used in manual assertion A type of epistatic interaction phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006097 functional complementation in heterologous system evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000064 functional complementation in heterologous system evidence A type of functional complementation evidence that is used in a manual assertion that is based on the insertion of a wild-type copy of a gene into a heterologous organism, with the mutation occurring in a homologous gene. http://purl.obolibrary.org/obo/ECO_0006098 temperature-sensitive mutant phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence resulting from altered gene function at higher temperatures. http://purl.obolibrary.org/obo/ECO_0006099 temperature-sensitive mutant phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000315 mutant phenotype evidence used in manual assertion A type of temperature-sensitive mutant phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006100 recessive mutant phenotype evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence based on the analysis of a mutation which, in diploid organisms, must be present in both alleles for a phenotype to manifest itself. http://purl.obolibrary.org/obo/ECO_0006101 recessive mutant phenotype evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000315 mutant phenotype evidence used in manual assertion A type of mutant phenotype evidence that is used in a manual assertion based on the analysis of a mutation which, in diploid organisms, must be present in both alleles for a phenotype to manifest itself. http://purl.obolibrary.org/obo/ECO_0007634 experimental phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of experimental phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007635 experimental phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of experimental phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007648 manually integrated combinatorial computational evidence http://purl.obolibrary.org/obo/ECO_0007677 combinatorial computational evidence A type of manually integrated combinatorial evidence in which two or more distinct types of computational evidence are integrated manually. http://purl.obolibrary.org/obo/ECO_0007649 manually integrated combinatorial computational evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007678 combinatorial computational evidence used in manual assertion A type of manually integrated combinatorial computational evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007650 manually integrated combinatorial computational evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007679 combinatorial computational evidence used in automatic assertion A type of manually integrated combinatorial computational evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007651 automatically integrated combinatorial computational evidence http://purl.obolibrary.org/obo/ECO_0007677 combinatorial computational evidence A type of automatically integrated combinatorial evidence in which two or more distinct types of computational evidence are integrated automatically. http://purl.obolibrary.org/obo/ECO_0007652 automatically integrated combinatorial computational evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000245 automatically integrated combinatorial evidence used in manual assertion A type of automatically integrated combinatorial computational evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007653 automatically integrated combinatorial computational evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000053 automatically integrated combinatorial evidence used in automatic assertion A type of automatically integrated combinatorial computational evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007654 combinatorial experimental evidence http://purl.obolibrary.org/obo/ECO_0000212 combinatorial evidence A type of combinatorial evidence in which two or more distinct types of experimental evidence are integrated. http://purl.obolibrary.org/obo/ECO_0007655 manually integrated combinatorial experimental evidence http://purl.obolibrary.org/obo/ECO_0007674 manually integrated combinatorial evidence A type of manually integrated combinatorial evidence in which two or more distinct types of experimental evidence are integrated manually. http://purl.obolibrary.org/obo/ECO_0007656 manually integrated combinatorial experimental evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007675 manually integrated combinatorial evidence used in manual assertion A type of manually integrated combinatorial experimental evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007657 manually integrated combinatorial experimental evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007676 manually integrated combinatorial evidence used in automatic assertion A type of manually integrated combinatorial experimental evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007658 automatically integrated combinatorial experimental evidence http://purl.obolibrary.org/obo/ECO_0007673 automatically integrated combinatorial evidence A type of automatically integrated combinatorial evidence in which two or more distinct types of experimental evidence are integrated automatically. http://purl.obolibrary.org/obo/ECO_0007659 automatically integrated combinatorial experimental evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000245 automatically integrated combinatorial evidence used in manual assertion A type of automatically integrated combinatorial experimental evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007660 automatically integrated combinatorial experimental evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000053 automatically integrated combinatorial evidence used in automatic assertion A type of automatically integrated combinatorial experimental evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007661 combinatorial computational and experimental evidence http://purl.obolibrary.org/obo/ECO_0000212 combinatorial evidence A type of combinatorial evidence in which at least one line of experimental evidence and at least one line of computational evidence have been integrated. http://purl.obolibrary.org/obo/ECO_0007662 manually integrated combinatorial computational and experimental evidence http://purl.obolibrary.org/obo/ECO_0007674 manually integrated combinatorial evidence A type of manually integrated combinatorial evidence in which at least one line of experimental evidence and at least one line of computational evidence have been integrated manually. http://purl.obolibrary.org/obo/ECO_0007663 manually integrated combinatorial computational and experimental evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007675 manually integrated combinatorial evidence used in manual assertion A type of manually integrated combinatorial computational and experimental evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007664 manually integrated combinatorial computational and experimental evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007676 manually integrated combinatorial evidence used in automatic assertion A type of manually integrated combinatorial computational and experimental evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007665 automatically integrated combinatorial computational and experimental evidence http://purl.obolibrary.org/obo/ECO_0007673 automatically integrated combinatorial evidence A type of automatically integrated combinatorial evidence in which at least one line of experimental evidence and at least one line of computational evidence have been integrated automatically. http://purl.obolibrary.org/obo/ECO_0007667 automatically integrated combinatorial computational and experimental evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000053 automatically integrated combinatorial evidence used in automatic assertion A type of automatically integrated combinatorial computational and experimental evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007668 computational evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000352 evidence used in manual assertion A type of computational evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007669 computational evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000501 evidence used in automatic assertion A type of computational evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007672 computational evidence http://purl.obolibrary.org/obo/ECO_0000000 evidence A type of evidence in which data are produced, and/or generated, and/or analyzed on a computer. http://purl.obolibrary.org/obo/ECO_0007673 automatically integrated combinatorial evidence http://purl.obolibrary.org/obo/ECO_0000212 combinatorial evidence A type of combinatorial evidence in which at least two distinct types of evidence have been integrated automatically. http://purl.obolibrary.org/obo/ECO_0007674 manually integrated combinatorial evidence http://purl.obolibrary.org/obo/ECO_0000212 combinatorial evidence A type of combinatorial evidence in which at least two distinct types of evidence have been integrated manually. http://purl.obolibrary.org/obo/ECO_0007675 manually integrated combinatorial evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000244 combinatorial evidence used in manual assertion A type of manually integrated combinatorial evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007676 manually integrated combinatorial evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000213 combinatorial evidence used in automatic assertion A type of manually integrated combinatorial evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007677 combinatorial computational evidence http://purl.obolibrary.org/obo/ECO_0000212 combinatorial evidence A type of combinatorial evidence in which at least two distinct types of computational evidence have been integrated. http://purl.obolibrary.org/obo/ECO_0007678 combinatorial computational evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000244 combinatorial evidence used in manual assertion A type of combinatorial computational evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007679 combinatorial computational evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000213 combinatorial evidence used in automatic assertion A type of combinatorial computational evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/GO_0019904 protein domain specific binding http://purl.obolibrary.org/obo/GO_0005515 protein binding Interacting selectively and non-covalently with a specific domain of a protein. http://purl.obolibrary.org/obo/GO_0043565 sequence-specific DNA binding http://purl.obolibrary.org/obo/GO_0003677 DNA binding Interacting selectively and non-covalently with DNA of a specific nucleotide composition, e.g. GC-rich DNA binding, or with a specific sequence motif or type of DNA e.g. promotor binding or rDNA binding. http://purl.obolibrary.org/obo/OBI_0002754 cDNA library http://purl.obolibrary.org/obo/OBI_0000047 processed material A collection of DNA molecules with sequences complementary to a specified set of mRNA molecules and commonly developed by enzymatic reverse transcription. http://purl.obolibrary.org/obo/OBI_2000009 bodily fluid specimen http://purl.obolibrary.org/obo/OBI_0001479 specimen from organism A specimen that is derived from some bodily fluid http://purl.obolibrary.org/obo/ECO_0008000 machine learning method evidence http://purl.obolibrary.org/obo/ECO_0007672 computational evidence A type of computational evidence in which computational systems build models based on sample data, known as training data, in order to make predictions or decisions without being explicitly programmed to do so. Such systems are trained on a combination of existing data about an entity, and are applied to the generation of new labels, such as numerical or textual or categorical. http://purl.obolibrary.org/obo/ECO_0008001 deep learning neural network method evidence http://purl.obolibrary.org/obo/ECO_0008013 neural network method evidence A type of neural network method evidence which leverages a broad suite of neural network models, which use a combination of data attributes of an entity called features as inputs, and map them to outputs through multiple layers of connected hidden units, whose parameters are learned from data. http://purl.obolibrary.org/obo/ECO_0008002 rule-based machine learning method evidence http://purl.obolibrary.org/obo/ECO_0008000 machine learning method evidence A type of machine learning method evidence which uses a combination of data attributes to derive knowledge used to create a set of rules. These rules collectively represent the prediction model and are in the form of "IF:THEN" statements. The knowledge applied can be traced back to the rules and the original data. http://purl.obolibrary.org/obo/ECO_0008003 machine learning method evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008000 machine learning method evidence A type of machine learning method evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008004 machine learning method evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008000 machine learning method evidence A type of machine learning method evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008005 deep learning neural network method evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008024 neural network method evidence used in manual assertion A type of deep learning neural network method evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008006 deep learning neural network method evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008025 neural network method evidence used in automatic assertion A type of deep learning neural network method evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008007 rule-based machine learning method evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008003 machine learning method evidence used in manual assertion A type of rule-based machine learning method evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008008 rule-based machine learning method evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008004 machine learning method evidence used in automatic assertion A type of rule-based machine learning method evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008030 intrinsic disorder prediction evidence http://purl.obolibrary.org/obo/ECO_0007672 computational evidence A type of computational evidence where one or more computational tools are used to predict regions of intrinsic disorder in a protein sequence. http://purl.obolibrary.org/obo/ECO_0008031 author inference based on intrinsic disorder prediction http://purl.obolibrary.org/obo/ECO_0000212 combinatorial evidence A type of author inference in which information from intrinsic disorder prediction tools has been employed. http://purl.obolibrary.org/obo/ECO_0008032 curator inference based on intrinsic disorder prediction http://purl.obolibrary.org/obo/ECO_0000212 combinatorial evidence A type of curator inference in which information from intrinsic disorder prediction tools has been employed. http://purl.obolibrary.org/obo/ECO_0008033 intrinsic disorder prediction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008030 intrinsic disorder prediction evidence A type of intrinsic disorder prediction evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008034 intrinsic disorder prediction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008030 intrinsic disorder prediction evidence A type of intrinsic disorder prediction evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008035 author inference based on intrinsic disorder prediction used in manual assertion http://purl.obolibrary.org/obo/ECO_0000244 combinatorial evidence used in manual assertion A type of author inference based on intrinsic disorder prediction that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008036 author inference based on intrinsic disorder prediction used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000213 combinatorial evidence used in automatic assertion A type of author inference based on intrinsic disorder prediction that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008037 curator inference based on intrinsic disorder prediction used in manual assertion http://purl.obolibrary.org/obo/ECO_0000305 curator inference used in manual assertion A type of curator inference based on intrinsic disorder prediction that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008038 curator inference based on intrinsic disorder prediction used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007322 curator inference used in automatic assertion A type of curator inference based on intrinsic disorder prediction that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0000024 protein binding evidence A type of protein binding evidence that employs specific antibodies to determine that a specific MHC protein complex or group of MHC protein complexes presented a ligand. http://purl.obolibrary.org/obo/ECO_0008040 antibody 10.3.6-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody 10.3.6 to determine that the H2-IA protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008041 antibody 14.4.4-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody 14.4.4 to determine that the H2-IAg7 protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008042 antibody 28-14-8S-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody 28-14-8S to determine that the H2-Ld protein complex, H2-Db protein complex, and/or H2-Dq protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008043 antibody 34-1-2S-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody 34-1-2S to determine that the mouse MHC class I protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008044 antibody 34-5-8S-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody 34-5-8S to determine that the H2-Dd protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008045 antibody 34-7-23S-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody 34-7-23S to determine that the H2-Dd protein complex and/or H2-Kd protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008046 antibody 4B7/8-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody 4B7/8 to determine that the pig MHC class I protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008047 antibody AG2.42.7-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody AG2.42.7 to determine that the H2-IAg7 protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008048 antibody B123.2-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody B123.2 to determine that the human MHC class I protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008049 antibody B22-249.R1-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody B22-249.R1 to determine that the H2-Db protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008050 antibody B7/21-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody B7/21 to determine that the HLA-DP protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008051 antibody B8.11.2-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody B8.11.2 to determine that the HLA-DR protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008052 antibody B8-24-3-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody B8-24-3 to determine that the mouse MHC class I protein complex with H2-b haplotype and/or mouse MHC protein complex with H2-p haplotype presented a ligand. http://purl.obolibrary.org/obo/ECO_0008053 antibody BB7.2-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody BB7.2 to determine that HLA protein complex with A2 serotype presented a ligand. http://purl.obolibrary.org/obo/ECO_0008054 antibody C7709A2-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody C7709A2 to determine that HLA protein complex with A24 serotype presented a ligand. http://purl.obolibrary.org/obo/ECO_0008055 antibody CR11-351-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody CR11-351 to determine that HLA protein complex with A2 serotype and/or HLA protein complex with A28 serotype presented a ligand. http://purl.obolibrary.org/obo/ECO_0008056 antibody DK1G8-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody DK1G8 to determine that HLA protein complex with A29 serotype presented a ligand. http://purl.obolibrary.org/obo/ECO_0008057 antibody DT9-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody DT9 to determine that the HLA-C protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008058 antibody HB145-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody HB145 to determine that the human MHC class II protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008059 antibody HB-164-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody HB-164 to determine that the human MHC class I protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008060 antibody HB180-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody HB180 to determine that the human MHC class II protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008061 antibody ILA-21-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody ILA-21 to determine that the cattle MHC class II protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008062 antibody ILA-88-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody ILA-88 to determine that the cattle MHC class I protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008063 antibody IVD12-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody IVD12 to determine that HLA protein complex with DQ3 serotype presented a ligand. http://purl.obolibrary.org/obo/ECO_0008064 antibody L243-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody L243 to determine that the HLA-DR protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008065 antibody LB3.1-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody LB3.13 to determine that the HLA-DR protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008066 antibody M1/42-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody M1/42 to determine that the mouse MHC class I protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008067 antibody M5/114-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody M5/114 to determine that the mouse MHC class II protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008068 antibody ME1-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody ME1 to determine that the HLA protein complex with B27 serotype presented a ligand. http://purl.obolibrary.org/obo/ECO_0008069 antibody MKD6-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody MKD6 to determine that the H2-IAd protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008070 antibody OX18-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody OX18 to determine that the rat MHC class I protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008071 antibody PT85-A-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody PT85-A to determine that the pig MHC class I protein complex, cattle MHC class I protein complex, and/or human MHC class I protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008072 antibody SF1-1.1.1-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody SF1-1.1.1 to determine that the H2-Kd protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008073 antibody SPVL3-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody SPVL3 to determine that the human MHC class II protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008074 antibody Tu39-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody Tu39 to determine that the human MHC class II protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008075 antibody W6/32-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody W6/32 to determine that the MHC class I protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008076 antibody Y-3-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody Y-3 to determine that theH2-Kb protein complex, H2-Ks protein complex, and/or H2-Kq protein complex presented a ligand. http://purl.obolibrary.org/obo/ECO_0008077 antibody Y3JP-specificity based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that employs antibody Y3JP to determine that the mouse MHC class II protein complex and/or rat MHC class II protein complex with RT1-A haplotype presented a ligand. http://purl.obolibrary.org/obo/ECO_0008078 protein expression-based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0000010 protein expression evidence A type of protein expression evidence that employs MHC molecule motif or alleles present to determine that a specific MHC molecule or class of MHC molecules presented a ligand. http://purl.obolibrary.org/obo/ECO_0008079 specific MHC protein expression-based MHC presentation evidence http://purl.obolibrary.org/obo/ECO_0008078 protein expression-based MHC presentation evidence A type of protein expression-based MHC presentation evidence that employs knowledge of a single specific MHC molecule's presence to determine that a specific MHC molecule presented a ligand. http://purl.obolibrary.org/obo/ECO_0008080 MHC:ligand binding based MHC restriction evidence http://purl.obolibrary.org/obo/ECO_0000024 protein binding evidence A type of protein binding evidence that employs an assay measuring binding of an MHC molecule to a ligand to determine that T cells can recognize the ligand when presented by that MHC molecule. http://purl.obolibrary.org/obo/ECO_0008081 MHC complex binding prediction based MHC restriction evidence http://purl.obolibrary.org/obo/ECO_0007672 computational evidence A type of computational evidence that employs a computational tool to predict MHC complex binding to a ligand to infer that T cells can recognize the ligand when presented by that MHC molecule. http://purl.obolibrary.org/obo/ECO_0008082 inference of MHC restriction based on shared MHC expression http://purl.obolibrary.org/obo/ECO_0000361 inferential evidence A type of inferential evidence that employs the association of specific MHC molecule expression by a subject population with a T cell response to infer that T cells can recognize the ligand when presented by that MHC molecule. http://purl.obolibrary.org/obo/ECO_0008083 T cell epitope assay type based MHC restriction evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence that employs the type of T cell epitope assay to determine that a specific ligand is restricted by a MHC molecule or class of MHC molecules. http://purl.obolibrary.org/obo/ECO_0008084 MHC-specific blocking based MHC restriction evidence http://purl.obolibrary.org/obo/ECO_0000024 protein binding evidence A type of protein binding evidence that employs the specific blocking of MHC molecules to determine that a ligand is restricted by that MHC molecule or class of MHC molecules. http://purl.obolibrary.org/obo/ECO_0008085 specific MHC molecule based MHC restriction evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence that employs the use of a specific MHC molecule in a T cell response assay to determine that a specific ligand is restricted by that MHC molecule. http://purl.obolibrary.org/obo/ECO_0008086 T cell type based MHC restriction evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence that employs a specific T cell type (CD4+ or CD8+) to determine that a ligand is restricted by a class of MHC molecules. http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008039 antibody-specificity based MHC presentation evidence A type of antibody-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007373 protein binding evidence used in automatic assertion A type of antibody-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008089 antibody 10.3.6-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody 10.3.6-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008090 antibody 10.3.6-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody 10.3.6-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008091 antibody 14.4.4-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody 14.4.4-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008092 antibody 14.4.4-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody 14.4.4-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008093 antibody 28-14-8S-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody 28-14-8S-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008094 antibody 28-14-8S-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody 28-14-8S-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008095 antibody 34-1-2S-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody 34-1-2S-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008096 antibody 34-1-2S-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody 34-1-2S-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008097 antibody 34-5-8S-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody 34-5-8S-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008098 antibody 34-5-8S-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody 34-5-8S-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008099 antibody 34-7-23S-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody 34-7-23S-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008100 antibody 34-7-23S-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody 34-7-23S-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008101 antibody 4B7/8-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody 4B7/8-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008102 antibody 4B7/8-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody 4B7/8-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008103 antibody AG2.42.7-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody AG2.42.7-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008104 antibody AG2.42.7-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody AG2.42.7-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008105 antibody B123.2-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody B123.2-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008106 antibody B123.2-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody B123.2-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008107 antibody B22-249.R1-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody B22-249.R1-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008108 antibody B22-249.R1-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody B22-249.R1-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008109 antibody B7/21-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody B7/21-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008110 antibody B7/21-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody B7/21-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008111 antibody B8.11.2-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody B8.11.2-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008112 antibody B8.11.2-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody B8.11.2-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008113 antibody B8-24-3-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody B8-24-3-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008114 antibody B8-24-3-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody B8-24-3-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008115 antibody BB7.2-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody BB7.2-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008116 antibody BB7.2-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody BB7.2-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008117 antibody C7709A2-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody C7709A2-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008118 antibody C7709A2-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody C7709A2-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008119 antibody CR11-351-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody CR11-351-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008120 antibody CR11-351-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody CR11-351-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008121 antibody DK1G8-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody DK1G8-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008122 antibody DK1G8-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody DK1G8-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008123 antibody DT9-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody DT9-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008124 antibody DT9-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody DT9-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008125 antibody HB145-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody HB145-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008126 antibody HB145-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody HB145-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008127 antibody HB-164-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody HB-164-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008128 antibody HB-164-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody HB-164-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008129 antibody HB180-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody HB180-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008130 antibody HB180-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody HB180-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008131 antibody ILA-21-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody ILA-21-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008132 antibody ILA-21-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody ILA-21-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008133 antibody ILA-88-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody ILA-88-specificity based MHC presentation evidence that is used in a manual assertion http://purl.obolibrary.org/obo/ECO_0008134 antibody ILA-88-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody ILA-88-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008135 antibody IVD12-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody IVD12-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008136 antibody IVD12-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody IVD12-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008137 antibody L243-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody L243-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008138 antibody L243-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody L243-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008139 antibody LB3.1-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody LB3.1-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008140 antibody LB3.1-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody LB3.1-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008141 antibody M1/42-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody M1/42-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008142 antibody M1/42-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody M1/42-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008143 antibody M5/114-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody M5/114-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008144 antibody M5/114-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody M5/114-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008145 antibody ME1-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody ME1-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008146 antibody ME1-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody ME1-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008147 antibody MKD6-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody MKD6-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008148 antibody MKD6-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody MKD6-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008149 antibody OX18-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody OX18-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008150 antibody OX18-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody OX18-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008151 antibody PT85-A-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody PT85-A-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008152 antibody PT85-A-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody PT85-A-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008153 antibody SF1-1.1.1-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody SF1-1.1.1-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008154 antibody SF1-1.1.1-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody SF1-1.1.1-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008155 antibody SPVL3-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody SPVL3-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008156 antibody SPVL3-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody SPVL3-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008157 antibody Tu39-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody Tu39-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008158 antibody Tu39-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody Tu39-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008159 antibody W6/32-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody W6/32-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008160 antibody W6/32-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody W6/32-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008161 antibody Y-3-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody Y-3-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008162 antibody Y-3-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody Y-3-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008163 antibody Y3JP-specificity based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008087 antibody-specificity based MHC presentation evidence used in manual assertion A type of antibody Y3JP-specificity based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008164 antibody Y3JP-specificity based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008088 antibody-specificity based MHC presentation evidence used in automatic assertion A type of antibody Y3JP-specificity based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008165 protein expression-based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000284 protein expression evidence used in manual assertion A type of protein expression-based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008166 protein expression-based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007309 protein expression evidence used in automatic assertion A type of protein expression-based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008167 specific MHC protein expression-based MHC presentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008165 protein expression-based MHC presentation evidence used in manual assertion A type of specific MHC protein expression-based MHC presentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008168 specific MHC protein expression-based MHC presentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008166 protein expression-based MHC presentation evidence used in automatic assertion A type of specific MHC protein expression-based MHC presentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008169 MHC:ligand binding based MHC restriction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008080 MHC:ligand binding based MHC restriction evidence A type of MHC:ligand binding based MHC restriction evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008170 MHC:ligand binding based MHC restriction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007373 protein binding evidence used in automatic assertion A type of MHC:ligand binding based MHC restriction evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008171 MHC complex binding prediction based MHC restriction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008081 MHC complex binding prediction based MHC restriction evidence A type of MHC complex binding prediction based MHC restriction evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008172 MHC complex binding prediction based MHC restriction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008081 MHC complex binding prediction based MHC restriction evidence A type of MHC complex binding prediction based MHC restriction evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008173 inference of MHC restriction based on shared MHC expression used in manual assertion http://purl.obolibrary.org/obo/ECO_0008082 inference of MHC restriction based on shared MHC expression A type of inference of MHC restriction based on shared MHC expression that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008174 inference of MHC restriction based on shared MHC expression used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008082 inference of MHC restriction based on shared MHC expression A type of inference of MHC restriction based on shared MHC expression that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008175 T cell epitope assay type based MHC restriction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of T cell epitope assay type based MHC restriction evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008176 T cell epitope assay type based MHC restriction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of T cell epitope assay type based MHC restriction evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008177 MHC-specific blocking based MHC restriction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008084 MHC-specific blocking based MHC restriction evidence A type of MHC-specific blocking based MHC restriction evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008178 MHC-specific blocking based MHC restriction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007373 protein binding evidence used in automatic assertion A type of MHC-specific blocking based MHC restriction evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008179 specific MHC molecule based MHC restriction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of specific MHC molecule based MHC restriction evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008180 specific MHC molecule based MHC restriction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of specific MHC molecule based MHC restriction evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008181 T cell type based MHC restriction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of T cell type based MHC restriction evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008182 T cell type based MHC restriction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of T cell type based MHC restriction evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008183 protein proximity labeling assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence in which proteins of interest are fused to an enzyme that is able to label or modify other proteins that come into the proximity of the protein of interest. Labeled/modified proteins are then isolated and identified, often by mass spectrometry. http://purl.obolibrary.org/obo/ECO_0008184 proximity-dependent biotin identification evidence http://purl.obolibrary.org/obo/ECO_0008183 protein proximity labeling assay evidence A type of protein proximity labeling assay evidence in which a mutant of a biotin protein ligase is fused to a protein of interest such that when other proteins enter the proximity of the protein of interest they are biotinylated. Biotinylated proteins are then isolated and identified, often by mass spectrometry. http://purl.obolibrary.org/obo/ECO_0008185 avidity evidence http://purl.obolibrary.org/obo/ECO_0000021 physical interaction evidence A type of physical interaction evidence that is the accumulated strength of multiple affinities of individual non-covalent binding interactions, such as between a protein receptor and its ligand. Avidity should not be thought of as the mere sum of its constituent affinities but as the combined effect of all affinities participating in the biomolecular interaction. It is commonly referred to as functional affinity. http://purl.obolibrary.org/obo/ECO_0008186 avidity-based extracellular interaction screening evidence http://purl.obolibrary.org/obo/ECO_0008185 avidity evidence A type of avidity evidence in which immobilized "bait" proteins are probed with enzyme-tagged, multimerized "prey" proteins to amplify avidity, enabling sensitive detection of otherwise weak and transient extracellular protein-protein interactions. http://purl.obolibrary.org/obo/ECO_0008187 single-molecule fluorescence resonance energy transfer evidence http://purl.obolibrary.org/obo/ECO_0001048 fluorescence resonance energy transfer evidence A type of fluorescence resonance energy transfer evidence resulting from the measurement of the distance-dependent energy transfer of two fluorophores attached to individual molecules, allowing for the detection of conformational states at both spatial and temporal resolution. http://purl.obolibrary.org/obo/ECO_0008188 protein proximity labeling assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of protein proximity labeling assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008189 protein proximity labeling assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of protein proximity labeling assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008190 proximity-dependent biotin identification evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008188 protein proximity labeling assay evidence used in manual assertion A type of proximity-dependent biotin identification evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008191 proximity-dependent biotin identification evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008189 protein proximity labeling assay evidence used in automatic assertion A type of proximity-dependent biotin identification evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008192 avidity evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008185 avidity evidence A type of avidity evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008193 avidity evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007332 physical interaction evidence used in automatic assertion A type of avidity evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008194 avidity-based extracellular interaction screening evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008192 avidity evidence used in manual assertion A type of avidity-based extracellular interaction screening evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008195 avidity-based extracellular interaction screening evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008193 avidity evidence used in automatic assertion A type of avidity-based extracellular interaction screening evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008196 single-molecule fluorescence resonance energy transfer evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001183 fluorescence resonance energy transfer evidence used in manual assertion A type of single-molecule fluorescence resonance energy transfer evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008197 single-molecule fluorescence resonance energy transfer evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007387 fluorescence resonance energy transfer evidence used in automatic assertion A type of single-molecule fluorescence resonance energy transfer evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000001 inference from background scientific knowledge http://purl.obolibrary.org/obo/ECO_0000205 curator inference A type of curator inference where conclusions are drawn based on the background scientific knowledge of the curator. http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence resulting from the direct measurement of some aspect of a biological feature. http://purl.obolibrary.org/obo/ECO_0000003 reconstitution assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence based on reconstructing a biological sample from its disassociated state to its original state. http://purl.obolibrary.org/obo/ECO_0000004 cell fractionation evidence http://purl.obolibrary.org/obo/ECO_0000100 fractionation evidence A type of fractionation evidence where sub-cellular components are separated based on their physical properties such as density in a sucrose density gradient. http://purl.obolibrary.org/obo/ECO_0000005 enzymatic activity assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of protein assay evidence where the catalytic activity of an enzyme is determined. http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence http://purl.obolibrary.org/obo/ECO_0000000 evidence A type of evidence that is the output of a scientific procedure performed to make a discovery, test a hypothesis, or demonstrate a known fact. http://purl.obolibrary.org/obo/ECO_0000007 immunofluorescence evidence http://purl.obolibrary.org/obo/ECO_0005517 protein detection assay evidence A type of protein detection assay evidence where a fluorescently labeled antibody is used to detect the presence or localization of a biomolecule within a cell. http://purl.obolibrary.org/obo/ECO_0000008 expression pattern evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence that is based on characterization of gene expression. http://purl.obolibrary.org/obo/ECO_0000009 transcript expression evidence http://purl.obolibrary.org/obo/ECO_0000008 expression pattern evidence A type of expression pattern evidence where abundance of a transcript is analyzed. http://purl.obolibrary.org/obo/ECO_0000010 protein expression evidence http://purl.obolibrary.org/obo/ECO_0000008 expression pattern evidence A type of expression pattern evidence resulting from protein abundance quantification techniques. http://purl.obolibrary.org/obo/ECO_0000011 genetic interaction evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence resulting from the effect that a given gene has on another gene or genes, and the products. http://purl.obolibrary.org/obo/ECO_0000012 functional complementation evidence http://purl.obolibrary.org/obo/ECO_0000011 genetic interaction evidence A type of genetic interaction evidence where a wild-type copy of the gene in question is inserted into a mutant cell to see if it restores the wild-type phenotype in the mutant background. http://purl.obolibrary.org/obo/ECO_0000013 transgenic rescue experiment evidence http://purl.obolibrary.org/obo/ECO_0000012 functional complementation evidence A type of functional complementation evidence resulting from the introduction of a transgene to prevent, or "rescue" an organism from a condition. http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence in which an observable phenotypic difference results from a change or mutation in DNA. http://purl.obolibrary.org/obo/ECO_0000016 loss-of-function mutant phenotype evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence where a phenotype is associated with altered gene product which lacks the molecular function of the wild-type gene. http://purl.obolibrary.org/obo/ECO_0000017 ectopic expression evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence where a transgenic strain carrying the construct of a promoter cDNA fusion in which a gene of interest is driven by a defined promoter or enhancer is ectopically expressed in the defined pattern to characterize potential cellular properties and functions of a protein of interest. http://purl.obolibrary.org/obo/ECO_0000018 anti-sense experiment evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence where a phenotype is observed while expressing an anti-sense version of a gene product in a wild-type (for that gene product) background. http://purl.obolibrary.org/obo/ECO_0000019 RNAi evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence where an RNA construct is introduced into a cell and the expression of the gene bearing its complementary sequence is suppressed. http://purl.obolibrary.org/obo/ECO_0000020 protein inhibition evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence based on the inhibition of the molecular function of a protein. http://purl.obolibrary.org/obo/ECO_0000021 physical interaction evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of experimental evidence that is based on characterization of an interaction between a gene product and another molecule. http://purl.obolibrary.org/obo/ECO_0000022 co-purification evidence http://purl.obolibrary.org/obo/ECO_0000021 physical interaction evidence A type of physical interaction evidence where a cellular component subunit is isolated as part of purification of its larger complex. http://purl.obolibrary.org/obo/ECO_0000023 affinity evidence http://purl.obolibrary.org/obo/ECO_0000021 physical interaction evidence A type of physical interaction evidence that depends on the strength of the interaction between two entities. http://purl.obolibrary.org/obo/ECO_0000024 protein binding evidence http://purl.obolibrary.org/obo/ECO_0000023 affinity evidence A type of affinity evidence resulting from the binding of a molecule to a protein or protein complex. http://purl.obolibrary.org/obo/ECO_0000025 bait-prey hybrid interaction evidence http://purl.obolibrary.org/obo/ECO_0006351 bait-prey interaction evidence A type of protein binding evidence where proteins of interest (bait and prey) are covalently linked to incomplete fragments of a third protein (reporter) and expressed in vivo, at which time interaction between bait and prey proteins brings reporter fragments in close enough proximity to allow them to reform and become a functional reporter protein. http://purl.obolibrary.org/obo/ECO_0000027 structural similarity evidence http://purl.obolibrary.org/obo/ECO_0000057 phenotypic similarity evidence A type of similarity evidence based on structural similarity of an annotated gene or gene product to another gene or group of genes. http://purl.obolibrary.org/obo/ECO_0000028 motif similarity evidence http://purl.obolibrary.org/obo/ECO_0000202 match to sequence model evidence A type of match to sequence model evidence that is based on the presence of a recognized domain or motif in a gene product's (usually protein) primary sequence. http://purl.obolibrary.org/obo/ECO_0000029 match to InterPro member signature evidence http://purl.obolibrary.org/obo/ECO_0000202 match to sequence model evidence A type of match to sequence model evidence resulting from a positive match of a protein, or set of proteins to a predictive model (signature) in the InterPro database. http://purl.obolibrary.org/obo/ECO_0000030 BLAST evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005560 pairwise sequence alignment evidence used in manual assertion A type of BLAST evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000031 protein BLAST evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000208 protein BLAST evidence A type of protein BLAST evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000032 nucleotide BLAST evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000207 nucleotide BLAST evidence A type of nucleotide BLAST evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000033 author statement supported by traceable reference http://purl.obolibrary.org/obo/ECO_0000204 author statement A type of author statement in which the author makes a statement that is not supported by information in that particular publication, but rather can be traced to a reference cited by that publication. http://purl.obolibrary.org/obo/ECO_0000034 author statement without traceable support http://purl.obolibrary.org/obo/ECO_0000204 author statement A type of author statement that is not associated with results presented or a cited reference. http://purl.obolibrary.org/obo/ECO_0000035 no evidence data found http://purl.obolibrary.org/obo/ECO_0000205 curator inference A type of curator inference that results when research finds no evidence information in the scientific literature, at reference databases, or from other resources. http://purl.obolibrary.org/obo/ECO_0000038 transient rescue experiment evidence http://purl.obolibrary.org/obo/ECO_0000012 functional complementation evidence A type of functional complementation evidence resulting from the introduction of nucleic acids, which are not permanently incorporated into the genome, to temporarily prevent, or "rescue" an organism from a condition. http://purl.obolibrary.org/obo/ECO_0000040 immunological assay evidence http://purl.obolibrary.org/obo/ECO_0000023 affinity evidence A type of affinity evidence resulting from quantitation of the analyte which depends on the reaction of an antigen (analyte) and an antibody. http://purl.obolibrary.org/obo/ECO_0000041 similarity evidence http://purl.obolibrary.org/obo/ECO_0000000 evidence A type of evidence resulting from comparing likeness of distinct biological entities. http://purl.obolibrary.org/obo/ECO_0000042 gain-of-function mutant phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence resulting from an altered gene product which possesses a new molecular function or a new pattern of gene expression. http://purl.obolibrary.org/obo/ECO_0000044 sequence similarity evidence http://purl.obolibrary.org/obo/ECO_0000041 similarity evidence A type of similarity based on biomolecular sequence. http://purl.obolibrary.org/obo/ECO_0000045 spatial pattern of protein expression evidence http://purl.obolibrary.org/obo/ECO_0000010 protein expression evidence A type of protein expression evidence that accounts for the position of RNA translation to a protein product ranging in scale from differing tissues to regions of an anatomical structure. http://purl.obolibrary.org/obo/ECO_0000047 spatial pattern of transcript expression evidence http://purl.obolibrary.org/obo/ECO_0000009 transcript expression evidence A type of transcript expression evidence that accounts for the position of transcription ranging in scale from the position of genes on the chromosome to differing tissues to regions of an anatomical structure. http://purl.obolibrary.org/obo/ECO_0000049 reporter gene assay evidence http://purl.obolibrary.org/obo/ECO_0000008 expression pattern evidence A type of expression pattern evidence that is based on the expression pattern of a reporter gene. http://purl.obolibrary.org/obo/ECO_0000050 voucher specimen phenotypic analysis evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence based on an authors phenotypic description of a species (or higher-level group), which explicitly references an observation made of a voucher specimen (a specimen with a permanent museum catalog). http://purl.obolibrary.org/obo/ECO_0000051 genetic similarity evidence http://purl.obolibrary.org/obo/ECO_0000041 similarity evidence A type of similarity based on genotype without respect to expression. http://purl.obolibrary.org/obo/ECO_0000052 suppressor/enhancer interaction phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000011 genetic interaction evidence A type of genetic interaction evidence resulting from a second mutation that either suppresses or enhances the phenotype expressed from an original mutation. http://purl.obolibrary.org/obo/ECO_0000053 automatically integrated combinatorial evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000213 combinatorial evidence used in automatic assertion A type of automatically integrated combinatorial evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000054 double mutant phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence resulting from an experiment typically constructed to determine if two different genes have an observable genetic interaction (functional connection) as the result of a mutation occurring in the alleles of the two genes of interest. http://purl.obolibrary.org/obo/ECO_0000056 epistatic interaction phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000011 genetic interaction evidence A type of genetic interaction evidence resulting from the suppression of one allelic effect by an allele at another genetic locus. http://purl.obolibrary.org/obo/ECO_0000057 phenotypic similarity evidence http://purl.obolibrary.org/obo/ECO_0000041 similarity evidence A type of similarity based on the expression of a genotype in an environment. http://purl.obolibrary.org/obo/ECO_0000058 expression microarray evidence http://purl.obolibrary.org/obo/ECO_0000009 transcript expression evidence A type of transcript expression evidence resulting from simultaneous profiling of the expression levels of thousands of genes in a single experiment allowing analysis of genes and their networks. http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence that is based on an observable characteristic trait, which is the result of the expression of an organisms genotype in an environment. http://purl.obolibrary.org/obo/ECO_0000060 positional similarity evidence http://purl.obolibrary.org/obo/ECO_0000057 phenotypic similarity evidence A type of phenotypic similarity evidence based on the similarity of stucture locations or arrangements. http://purl.obolibrary.org/obo/ECO_0000061 quantitative trait analysis evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of phenotypic evidence that is based on a gene product that is associated with a quantitative trait locus, but has not been cloned. http://purl.obolibrary.org/obo/ECO_0000062 cRNA to DNA expression microarray evidence http://purl.obolibrary.org/obo/ECO_0000058 expression microarray evidence A type of expression microarray evidence where expression level is quantified by sample biotin-labeled cRNA (transcribed from an unknown RNA sample) hybridized to DNA oligonuclotides immoblized on a solid surface. http://purl.obolibrary.org/obo/ECO_0000063 compositional similarity evidence http://purl.obolibrary.org/obo/ECO_0000057 phenotypic similarity evidence A type of phenotypic similarity evidence based on the similarity of the histological makeup of structures. http://purl.obolibrary.org/obo/ECO_0000064 functional complementation in heterologous system evidence http://purl.obolibrary.org/obo/ECO_0000012 functional complementation evidence A type of functional complementation evidence that is based on the insertion of a wild-type copy of a gene into a heterologous organism, with the mutation occurring in a homologous gene. http://purl.obolibrary.org/obo/ECO_0000066 yeast one-hybrid evidence http://purl.obolibrary.org/obo/ECO_0000025 bait-prey hybrid interaction evidence A type of bait-prey hybrid interaction evidence that is based on a protein-DNA complementation assay where a single promoter acts as bait and is screened against a library of prey transcription factors. http://purl.obolibrary.org/obo/ECO_0000067 developmental similarity evidence http://purl.obolibrary.org/obo/ECO_0000057 phenotypic similarity evidence A type of phenotypic similarity evidence based on the similarity of embryological or post-embryonic orgin of structures. http://purl.obolibrary.org/obo/ECO_0000068 yeast 2-hybrid evidence http://purl.obolibrary.org/obo/ECO_0000025 bait-prey hybrid interaction evidence A type of bait-prey hybrid interaction evidence that is based on detection of protein-protein interaction by activation of a yeast reporter gene after a bait protein fused to a DNA-binding domain (which has been transfected into a yeast cell) is used to screen a cDNA library of clones fused to an activation domain. http://purl.obolibrary.org/obo/ECO_0000069 differential methylation hybridization evidence http://purl.obolibrary.org/obo/ECO_0001065 in vitro methylation assay evidence A type of in vitro methylation assay evidence where methylation-sensitive restriction enzymes are utilized to compare differential methylation between two samples by first shearing DNA, followed by end-blunting, ligation of linkers, methylation sensitive restriction, PCR using linker primers, dye labeling and relative quantification of methylated DNA fragments by two-colored array hybridization to a CpG island microarray for visual assessment. http://purl.obolibrary.org/obo/ECO_0000070 co-immunoprecipitation evidence http://purl.obolibrary.org/obo/ECO_0000085 immunoprecipitation evidence A type of immunoprecipitation evidence that involves precipitating two or more proteins via binding to an antibody specific to a single protein, followed by protein identification. http://purl.obolibrary.org/obo/ECO_0000071 morphological similarity evidence http://purl.obolibrary.org/obo/ECO_0000057 phenotypic similarity evidence A type of phenotypic similarity evidence based on the similarity of shape, structure, or configuration in structures. http://purl.obolibrary.org/obo/ECO_0000074 split-ubiquitin functional complementation evidence http://purl.obolibrary.org/obo/ECO_0006256 protein fragment functional complementation evidence A type of protein fragment functional complementation evidence that is based on detection of protein-protein interaction between a bait and prey protein by in vivo reconstitution of split-ubiquitin (when bait and prey interact) and release of a reporter protein. http://purl.obolibrary.org/obo/ECO_0000075 gene expression similarity evidence http://purl.obolibrary.org/obo/ECO_0000057 phenotypic similarity evidence A type of phenotypic similarity evidence that is based on the categorization of genes by the similarity of expression profiles. http://purl.obolibrary.org/obo/ECO_0000076 far-Western blotting evidence http://purl.obolibrary.org/obo/ECO_0000021 physical interaction evidence A type of physical interaction evidence that is based on detection of protein-protein interactions by separation of target proteins by SDS-PAGE which are blotted to a membrane, followed by denaturation and renaturation, probing with purified bait proteins, and detection of the target-bait complexes. http://purl.obolibrary.org/obo/ECO_0000077 methylation-specific polymerase chain reaction evidence http://purl.obolibrary.org/obo/ECO_0001065 in vitro methylation assay evidence A type of in vitro methylation assay evidence resulting from initial modification of DNA by sodium bisulfite, converting all unmethylated cytosines to uracil, and subsequent amplification with primers specific for methylated versus unmethylated DNA. http://purl.obolibrary.org/obo/ECO_0000078 southern hybridization evidence http://purl.obolibrary.org/obo/ECO_0005519 DNA detection assay evidence A type of DNA detection assay evidence that is based on detection of a specific DNA sequence by hybridization of labeled probes to any immobilized DNA fragment with sequence similarity. http://purl.obolibrary.org/obo/ECO_0000079 affinity chromatography evidence http://purl.obolibrary.org/obo/ECO_0000023 affinity evidence A type of affinity evidence that results from separation of biochemical mixtures by selective binding of a compound to an immobilized compound on a polymeric matrix, subsequent removal of unattached components, and then displacement of the bound compound. http://purl.obolibrary.org/obo/ECO_0000080 phylogenetic evidence http://purl.obolibrary.org/obo/ECO_0000041 similarity evidence A type of similarity that indicates common ancestry. http://purl.obolibrary.org/obo/ECO_0000081 targeting sequence prediction evidence http://purl.obolibrary.org/obo/ECO_0000028 motif similarity evidence A type of motif similarity evidence that is based on detection of a targeting sequence in the primary sequence of a protein through computational prediction and/or a manual examination of the sequence. http://purl.obolibrary.org/obo/ECO_0000083 transmembrane domain prediction evidence http://purl.obolibrary.org/obo/ECO_0000028 motif similarity evidence A type of motif similarity evidence that is based on detection of one, or more, transmembrane domains in the primary sequence of a protein through computational prediction and/or a manual examination of the sequence. http://purl.obolibrary.org/obo/ECO_0000084 gene neighbors evidence http://purl.obolibrary.org/obo/ECO_0000177 genomic context evidence A type of genomic context evidence in which a gene product's identity is supported on the basis of the identity of neighboring genes. http://purl.obolibrary.org/obo/ECO_0000085 immunoprecipitation evidence http://purl.obolibrary.org/obo/ECO_0000024 protein binding evidence A type of protein binding evidence where an antibody specific for a target protein is used to precipitate the target protein along with any other molecules (e.g. protein, nucleic acid) that are bound to the target protein. http://purl.obolibrary.org/obo/ECO_0000086 amplification of intermethylated sites evidence http://purl.obolibrary.org/obo/ECO_0001065 in vitro methylation assay evidence A type of in vitro methylation assay evidence resulting from a genome-wide estimate of DNA methylation by differential enzymatic digestion of genomic DNA with methylation-sensitive and methylation-insensitive isoschizomers, followed by restrained PCR amplification of sequences methylated at both ends and resolving the PCR products in a denaturing polyacrylamide-sequencing gel to generate fingerprints that consist of multiple anonymous bands that represent the DNA methylome of the cell. http://purl.obolibrary.org/obo/ECO_0000087 immunolocalization evidence http://purl.obolibrary.org/obo/ECO_0005593 immunodetection assay evidence A type of microscopy evidence where antibodies are used to detect the location of molecules or other structures within cells or tissues. http://purl.obolibrary.org/obo/ECO_0000088 biological system reconstruction evidence http://purl.obolibrary.org/obo/ECO_0000205 curator inference A type of curator inference that is based on a combination of experimental evidences or existing models of that system in a related species used for the reconstruction of a biological system. http://purl.obolibrary.org/obo/ECO_0000089 restriction landmark genomic scanning evidence http://purl.obolibrary.org/obo/ECO_0005519 DNA detection assay evidence A type of DNA detection assay evidence resulting from the use of restriction enzymes for direct end-labeling of DNA (creating landmarks), followed by high-resolution two-dimensional electrophoresis to visualize the landmarks. http://purl.obolibrary.org/obo/ECO_0000090 immunogold labelling evidence http://purl.obolibrary.org/obo/ECO_0000087 immunolocalization evidence A type of immunolocalization evidence where the antibodies are labeled with colloidal gold particles whose location is then detected via microscopy. http://purl.obolibrary.org/obo/ECO_0000092 epitope-tagged protein immunolocalization evidence http://purl.obolibrary.org/obo/ECO_0000087 immunolocalization evidence A type of immunolocalization evidence in which recombinant proteins fused with epitopes are recognized by antibodies. http://purl.obolibrary.org/obo/ECO_0000093 array-based sequence capture evidence http://purl.obolibrary.org/obo/ECO_0005519 DNA detection assay evidence A type of DNA detection assay evidence evidence where gene sequence or variation is determined or detected by fluorescently-labeled DNA fragments hybridized to sequence-specific oligonucleotides immobilized on an array. http://purl.obolibrary.org/obo/ECO_0000095 cell growth regulation assay evidence http://purl.obolibrary.org/obo/ECO_0001563 cell growth assay evidence A type of cell growth assay evidence that measures one or more aspects of cell growth over a specified time period to indicate an induced or repressed regulatory effect. http://purl.obolibrary.org/obo/ECO_0000096 electrophoretic mobility shift assay evidence http://purl.obolibrary.org/obo/ECO_0000021 physical interaction evidence A type of affinity evidence based on an electrophoretic mobility shift of macromolecules, where proteins, nucleic acids, or both, are combined and the resulting mixtures are electrophoresed under native conditions through polyacrylamide or agarose gel to detect interactions/complexes between proteins and/or nucleic acid. http://purl.obolibrary.org/obo/ECO_0000097 cDNA to DNA expression microarray evidence http://purl.obolibrary.org/obo/ECO_0000058 expression microarray evidence A type of expression microarray evidence where expression level is quantified by fluroescently-labeled cDNA (reverse transcribed from an unknown RNA sample) hybridized to DNA immobilized on a solid surface. http://purl.obolibrary.org/obo/ECO_0000100 fractionation evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence resulting from the separation of various cell components while preserving their individual functions. http://purl.obolibrary.org/obo/ECO_0000101 Affymetrix GeneChip evidence http://purl.obolibrary.org/obo/ECO_0000062 cRNA to DNA expression microarray evidence A type of cRNA to DNA expression microarray evidence resulting from the use of a proprietary Affymetrix GeneChip System for analyzing complex genetic information. http://purl.obolibrary.org/obo/ECO_0000102 co-fractionation evidence http://purl.obolibrary.org/obo/ECO_0000100 fractionation evidence A type of fractionation evidence resulting from a protein fractionated with other compounds, factors, or macromolecules. http://purl.obolibrary.org/obo/ECO_0000104 DNA to cDNA expression microarray evidence http://purl.obolibrary.org/obo/ECO_0000058 expression microarray evidence A type of expression microarray evidence where expression levels are quantified by hybridization of fluorescently-labeled DNA to cDNA (reverse transcribed from an unknown RNA sample) immobilized on a solid surface. http://purl.obolibrary.org/obo/ECO_0000105 Nimblegen array evidence http://purl.obolibrary.org/obo/ECO_0000097 cDNA to DNA expression microarray evidence A type of cDNA to DNA expression microarray evidence resulting from the use of a proprietary NimbleGen array to measure expression levels. http://purl.obolibrary.org/obo/ECO_0000106 northern blot evidence http://purl.obolibrary.org/obo/ECO_0000009 transcript expression evidence A type of transcript expression evidence based on electrophoresis and probing to determine levels of RNA expression using a complementary hybridization probe on the separated RNA samples. http://purl.obolibrary.org/obo/ECO_0000109 reverse transcription polymerase chain reaction evidence http://purl.obolibrary.org/obo/ECO_0005518 RNA detection assay evidence A type of RNA detection assay evidence where an RNA transcript is reverse transcribed into cDNA and amplified to qualitatively detect gene expression. http://purl.obolibrary.org/obo/ECO_0000110 RNA protection assay evidence http://purl.obolibrary.org/obo/ECO_0001106 nuclease protection assay evidence A type of nuclease protection assay evidence where mRNA is hybridized with radiolabeled RNA probes after which RNAse is added to digest the unbound, nonresistant single-stranded overhang regions, later the remaining probe target hybrids are purified and resolved on denaturing polyacrylamide gel, and quantified by autoradiography. http://purl.obolibrary.org/obo/ECO_0000112 qualitative western immunoblotting evidence http://purl.obolibrary.org/obo/ECO_0005593 immunodetection assay evidence A type of protein expression evidence where proteins are separated using gel electrophoresis, blotted onto a membrane, and probed with an antibody that can be detected via light or radioactive emission. http://purl.obolibrary.org/obo/ECO_0000114 expression library screen evidence http://purl.obolibrary.org/obo/ECO_0000010 protein expression evidence A type of protein expression evidence where a protein of interest is isolated on the basis of its hybridization to an antibody raised to a homologous protein. http://purl.obolibrary.org/obo/ECO_0000116 differential hybridization evidence http://purl.obolibrary.org/obo/ECO_0000009 transcript expression evidence A type of nucleic acid hybridization evidence in which preferential or exclusive expression of genes in one cell type or tissue is compared to another cell type or tissue when mRNA from each sample is reverse transcribed, labeled, and hybridized to a cDNA library to compare hybridization patterns. http://purl.obolibrary.org/obo/ECO_0000118 subtractive hybridization evidence http://purl.obolibrary.org/obo/ECO_0000009 transcript expression evidence A type of nucleic acid hybridization evidence in which preferential or exclusive expression of genes in one cell type or tissue are compared to another cell type or tissue when denatured ds-cDNA from the two samples are hybridized, and the common sequences are 'subtracted'. http://purl.obolibrary.org/obo/ECO_0000120 over expression analysis evidence http://purl.obolibrary.org/obo/ECO_0005027 genetic transformation evidence A type of experimental phenotypic evidence in which a gene and/or gene product is investigated in a transgenic organism that has been engineered to overexpress that gene product. http://purl.obolibrary.org/obo/ECO_0000122 protein localization evidence http://purl.obolibrary.org/obo/ECO_0001533 localization evidence A type of localization evidence where sub-cellular localization of a protein is determined. http://purl.obolibrary.org/obo/ECO_0000124 fusion protein localization evidence http://purl.obolibrary.org/obo/ECO_0000122 protein localization evidence A type of protein localization evidence resulting from the fusion of a protein of interest to a labeling protein which has enzymatic activity or fluorescence properties. http://purl.obolibrary.org/obo/ECO_0000126 green fluorescent protein fusion protein localization evidence http://purl.obolibrary.org/obo/ECO_0000124 fusion protein localization evidence A type of fusion protein localization evidence resulting from the labeling of a protein of interest with green fluorescent protein (GFP) from the jellyfish Aequorea victoria. http://purl.obolibrary.org/obo/ECO_0000128 yellow fluorescent protein fusion protein localization evidence http://purl.obolibrary.org/obo/ECO_0000124 fusion protein localization evidence A type of fusion protein localization evidence resulting from the labeling of a protein of interest with yellow fluorescent protein (YFP), which was created by mutating green fluorescent protein (GFP) of the Aequorea victoria. http://purl.obolibrary.org/obo/ECO_0000130 beta-glucuronidase fusion protein localization evidence http://purl.obolibrary.org/obo/ECO_0000124 fusion protein localization evidence A type of fusion protein localization evidence resulting from the targeted insertion of the beta-glucuronidase (GUS) coding gene as a reporter gene for the localization of a particular gene product. http://purl.obolibrary.org/obo/ECO_0000132 beta-galactosidase fusion protein localization evidence http://purl.obolibrary.org/obo/ECO_0000124 fusion protein localization evidence A type of fusion protein localization evidence resulting from the targeted insertion of the beta-galactosidase coding gene (lacZ) into a specific gene to create a beta-galactosidase-tagged fusion protein. http://purl.obolibrary.org/obo/ECO_0000134 transport assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence resulting from the activity assessment of transport proteins. http://purl.obolibrary.org/obo/ECO_0000136 nucleic acid binding evidence http://purl.obolibrary.org/obo/ECO_0000023 affinity evidence A type of affinity evidence resulting from the binding of a molecule to a nucleic acid. http://purl.obolibrary.org/obo/ECO_0000138 ribohomopolymer binding assay evidence http://purl.obolibrary.org/obo/ECO_0000136 nucleic acid binding evidence A type of nucleic acid binding evidence resulting from an enzyme displaying binding activity to specific ribohomopolymer. http://purl.obolibrary.org/obo/ECO_0000140 thin layer chromatography evidence http://purl.obolibrary.org/obo/ECO_0000325 chromatography evidence A type of chromatography evidence that uses a thin layer of adsorbent (such as silica gel, alumina, or cellulose) on a flat, inert substrate. http://purl.obolibrary.org/obo/ECO_0000142 protein:ion binding evidence http://purl.obolibrary.org/obo/ECO_0000024 protein binding evidence A type of protein binding evidence resulting from a metal ion binding to a protein at a specific binding site. http://purl.obolibrary.org/obo/ECO_0000144 Southwestern blot evidence http://purl.obolibrary.org/obo/ECO_0000136 nucleic acid binding evidence A type of nucleic acid binding evidence in which DNA-protein binding is detected using labeled DNA as probes, hybridized to electrophoretically separated proteins. http://purl.obolibrary.org/obo/ECO_0000146 Northwestern blot evidence http://purl.obolibrary.org/obo/ECO_0000136 nucleic acid binding evidence A type of nucleic acid binding evidence in which RNA-protein binding is detected using labeled RNA as probes, hybridized to electrophoretically separated proteins. http://purl.obolibrary.org/obo/ECO_0000150 in vitro transcription reconstitution assay evidence http://purl.obolibrary.org/obo/ECO_0000003 reconstitution assay evidence A type of reconstitution assay evidence resulting from the use of reconstituted polymerase transcription systems to investigate RNA synthesis. http://purl.obolibrary.org/obo/ECO_0000152 in vitro recombinant protein transcription reconstitution assay evidence http://purl.obolibrary.org/obo/ECO_0000150 in vitro transcription reconstitution assay evidence A type of in vitro transcription reconstitution assay evidence resulting from the use of recombinant proteins in preparation of a fully-defined transcription system. http://purl.obolibrary.org/obo/ECO_0000154 heterologous protein expression evidence http://purl.obolibrary.org/obo/ECO_0000010 protein expression evidence A type of protein expression evidence where a gene from one cell is inserted into a cell that does not typically contain that gene and heterologous protein expression is assessed. http://purl.obolibrary.org/obo/ECO_0000156 protein separation evidence http://purl.obolibrary.org/obo/ECO_0000100 fractionation evidence A type of fractionation evidence resulting from the isolation of a single type of protein from a complex mixture. http://purl.obolibrary.org/obo/ECO_0000158 protein separation followed by direct sequencing evidence http://purl.obolibrary.org/obo/ECO_0000156 protein separation evidence A type of protein separation evidence that is followed by direct protein sequencing whereby a protein's amino acid sequence is determined experimentally by Edman degradation or by mass spectrometry. http://purl.obolibrary.org/obo/ECO_0000160 protein separation followed by fragment identification evidence http://purl.obolibrary.org/obo/ECO_0000156 protein separation evidence A type of protein separation evidence which then utilizes the screening and identification of small-molecules that bind to proteins. http://purl.obolibrary.org/obo/ECO_0000162 heterologous system uptake evidence http://purl.obolibrary.org/obo/ECO_0000134 transport assay evidence A type of transport assay evidence in which the uptake mechanism of a transporter is investigated in a cell that does not normally express that protein. http://purl.obolibrary.org/obo/ECO_0000164 electrophysiology assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where electrical properties of cells or tissues are studied. http://purl.obolibrary.org/obo/ECO_0000166 two-electrode voltage clamp recording evidence http://purl.obolibrary.org/obo/ECO_0005576 voltage clamp recording evidence A type of voltage clamp recording evidence which involves using two electrodes inserted into a large cell (often a xenopus oocyte) where one electrode is used to measure the internal potential (voltage) of the cell and the other electrode is used to clamp the current. http://purl.obolibrary.org/obo/ECO_0000168 transcription assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence resulting from the detection and quantification of transcribed RNA. http://purl.obolibrary.org/obo/ECO_0000170 transcriptional activation assay evidence http://purl.obolibrary.org/obo/ECO_0000168 transcription assay evidence A type of transcription assay evidence in which sequence-specific binding proteins that increase transcription of a specific target gene or genes are identified, quantified, and/or analyzed. http://purl.obolibrary.org/obo/ECO_0000172 biochemical trait analysis evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence in which the biochemical aspect of a mutation is analyzed. http://purl.obolibrary.org/obo/ECO_0000174 mutant physiological response evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence in which the physiological response of a mutant when presented with an external stimulus is determined. http://purl.obolibrary.org/obo/ECO_0000176 mutant visible phenotype evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence in which traits arising from a mutation are visually examined. http://purl.obolibrary.org/obo/ECO_0000177 genomic context evidence http://purl.obolibrary.org/obo/ECO_0000041 similarity evidence A type of similarity evidence in which the location of the gene within the genome provides insight into the gene's function or evolutionary insight. http://purl.obolibrary.org/obo/ECO_0000178 in vivo assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence resulting from a sample of microorganisms or living tissue grown in a medium. http://purl.obolibrary.org/obo/ECO_0000179 animal model system study evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence arising from the investigation of an animal model system. http://purl.obolibrary.org/obo/ECO_0000180 clinical study evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence arising from a controlled investigation that uses human subjects. http://purl.obolibrary.org/obo/ECO_0000181 in vitro assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence resulting from a process performed outside the living organism, where the components of an organism have been isolated from their usual biological context, to permit a more detailed or more convenient analysis in an artificial setting. http://purl.obolibrary.org/obo/ECO_0000183 cell-free assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence resulting from studies in which cytoplasmic and/or nuclear cellular components from resting, nonstimulated cells, are isolated by ultracentrifugation to provide molecular machinery that can be used in reactions in the absence of many of the other cellular components for the purpose of in vitro protein synthesis, transcription, DNA replication, etc. http://purl.obolibrary.org/obo/ECO_0000184 enzyme inhibition evidence http://purl.obolibrary.org/obo/ECO_0000020 protein inhibition evidence A type of protein inhibition evidence and enzymatic acitivty evidence where the protein under study is an enzyme. http://purl.obolibrary.org/obo/ECO_0000200 sequence alignment evidence http://purl.obolibrary.org/obo/ECO_0000044 sequence similarity evidence A type of sequence similarity evidence in which an annotation is made based on a manually-reviewed or published sequence alignment http://purl.obolibrary.org/obo/ECO_0000201 sequence orthology evidence http://purl.obolibrary.org/obo/ECO_0000044 sequence similarity evidence A type of sequence similarity evidence in which orthology is established by multiple criteria, including amino acid and/or nucleotide sequence comparisons and one or more of the following: phylogenetic analysis, coincident expression, conserved map location, functional complementation, immunological cross-reaction, similarity in subcellular localization, subunit structure, substrate specificity, and response to specific inhibitors. http://purl.obolibrary.org/obo/ECO_0000202 match to sequence model evidence http://purl.obolibrary.org/obo/ECO_0000044 sequence similarity evidence A type of sequence similarity evidence in which the function of a gene product is predicted based on a sequence-based statistical model. http://purl.obolibrary.org/obo/ECO_0000203 automatic assertion http://purl.obolibrary.org/obo/ECO_0000217 assertion method An assertion method that does not involve human review. http://purl.obolibrary.org/obo/ECO_0000204 author statement http://purl.obolibrary.org/obo/ECO_0006151 documented statement evidence A type of documented statement evidence that is based on an assertion by the author of a paper, which is read by a curator. http://purl.obolibrary.org/obo/ECO_0000205 curator inference http://purl.obolibrary.org/obo/ECO_0000361 inferential evidence A type of inferential evidence that is based on a conclusion drawn by a curator. http://purl.obolibrary.org/obo/ECO_0000206 BLAST evidence http://purl.obolibrary.org/obo/ECO_0005554 pairwise sequence alignment evidence A type of pairwise sequence alignment evidence obtained with basic local alignment search tool (BLAST). http://purl.obolibrary.org/obo/ECO_0000207 nucleotide BLAST evidence http://purl.obolibrary.org/obo/ECO_0000206 BLAST evidence A type of BLAST evidence in which nucleotide sequences are aligned. http://purl.obolibrary.org/obo/ECO_0000208 protein BLAST evidence http://purl.obolibrary.org/obo/ECO_0000206 BLAST evidence A type of BLAST evidence in which amino acid or translated nucleotide sequences are aligned. http://purl.obolibrary.org/obo/ECO_0000209 BLAST evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005561 pairwise sequence alignment evidence used in automatic assertion A type of BLAST evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000210 nucleotide BLAST evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000209 BLAST evidence used in automatic assertion A type of nucleotide BLAST evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000211 protein BLAST evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000209 BLAST evidence used in automatic assertion A type of protein BLAST evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000212 combinatorial evidence http://purl.obolibrary.org/obo/ECO_0000000 evidence A type of evidence in which at least two distinct types of evidence have been integrated. http://purl.obolibrary.org/obo/ECO_0000213 combinatorial evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000501 evidence used in automatic assertion A type of combinatorial analysis that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000214 biological aspect of descendant evidence http://purl.obolibrary.org/obo/ECO_0000080 phylogenetic evidence A type of phylogenetic evidence whereby an aspect of an ancestral gene is inferred through the characterization of an aspect of a descendant gene. http://purl.obolibrary.org/obo/ECO_0000215 rapid divergence from ancestral sequence evidence http://purl.obolibrary.org/obo/ECO_0000080 phylogenetic evidence A type of phylogenetic evidence characterized by long phylogenetic tree branch lengths following a duplication event. http://purl.obolibrary.org/obo/ECO_0000216 phylogenetic determination of loss of key residues evidence http://purl.obolibrary.org/obo/ECO_0000080 phylogenetic evidence A type of phylogenetic evidence characterized by the absence of key sequence residues. http://purl.obolibrary.org/obo/ECO_0000218 manual assertion http://purl.obolibrary.org/obo/ECO_0000217 assertion method An assertion method that involves human review. http://purl.obolibrary.org/obo/ECO_0000219 nucleotide sequencing assay evidence http://purl.obolibrary.org/obo/ECO_0000220 sequencing assay evidence A type of sequencing assay that determines the sequence of a biopolymer comprised of nucleotides. http://purl.obolibrary.org/obo/ECO_0000220 sequencing assay evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence where the order of molecules that constitute a biopolymer is determined. http://purl.obolibrary.org/obo/ECO_0000221 high throughput nucleotide sequencing assay evidence http://purl.obolibrary.org/obo/ECO_0000219 nucleotide sequencing assay evidence A type of nucleotide sequencing assay evidence in which a sequence is derived from a parallelized sequencing technique. http://purl.obolibrary.org/obo/ECO_0000222 Illumina sequencing evidence http://purl.obolibrary.org/obo/ECO_0000221 high throughput nucleotide sequencing assay evidence A type of high throughput nucleotide sequencing evidence in which sequence is determined through hybridization of adaptor-ligated DNA to a glass slide on which each fragment is simultaneously clonally amplified, followed by progressive rounds of base incorporation with fluorescently-tagged nucleotides, washing, imaging, and cleavage. http://purl.obolibrary.org/obo/ECO_0000223 454 pyrosequencing evidence http://purl.obolibrary.org/obo/ECO_0000221 high throughput nucleotide sequencing assay evidence A type of high throughput nucleotide sequencing evidence in which the sequence for a ssDNA is determined by addition of primers and then nucleotides, one base pair at a time (which generate a specific fluorescence), to the target strand. http://purl.obolibrary.org/obo/ECO_0000224 SOLiD sequencing evidence http://purl.obolibrary.org/obo/ECO_0000221 high throughput nucleotide sequencing assay evidence A type of high throughput nucleotide sequencing evidence in which sequence is determined through rounds of ligation-based sequencing with fluorescently-labeled Di-base probes and cleavage, after which the template is reset, with a primer offset by one base, for subsequent rounds of ligation. http://purl.obolibrary.org/obo/ECO_0000225 chain termination sequencing evidence http://purl.obolibrary.org/obo/ECO_0000219 nucleotide sequencing assay evidence A type of nucleotide sequencing assay in which DNA sequence is determined by addition of a primed DNA template to a reaction mixture, followed by addition of DNA polymerase, deoxynucleosidetriphosphates (dNTPs - one of which may be radiolabeled), and one of four di-deoxynucleotidetriphosphates (ddNTPs), after which DNA elongation is terminated and the DNA is separated by size and imaged. http://purl.obolibrary.org/obo/ECO_0000226 chromatin immunoprecipitation evidence http://purl.obolibrary.org/obo/ECO_0000085 immunoprecipitation evidence A type of immunoprecipitation evidence that is used to identify a protein binding site on a genomic DNA sequence. http://purl.obolibrary.org/obo/ECO_0000227 chromatin immunoprecipitation-PCR evidence http://purl.obolibrary.org/obo/ECO_0000226 chromatin immunoprecipitation evidence A type of chromatin immunoprecipitation evidence that uses polymerase chain reaction (PCR) where specific primers are used on the pulled-down DNA for DNA detection. http://purl.obolibrary.org/obo/ECO_0000228 chromatin immunoprecipitation-qPCR evidence http://purl.obolibrary.org/obo/ECO_0000226 chromatin immunoprecipitation evidence A type of chromatin immunoprecipitation evidence in which protein-DNA interactions are investigated at known genomic binding sites through a process of ChIP, DNA purification, and finally quantitative polymerase chain reaction (qPCR) for DNA detection. http://purl.obolibrary.org/obo/ECO_0000229 chromatin immunoprecipitation-seq evidence http://purl.obolibrary.org/obo/ECO_0000226 chromatin immunoprecipitation evidence A type of chromatin immunoprecipitation evidence that uses high-throughput sequencing where immunoprecipitated DNA fragments are funnelled into a massively parallel sequencer to produce multiple short reads for locating binding sites of DNA-associated proteins. http://purl.obolibrary.org/obo/ECO_0000230 chromatin immunoprecipitation-chip evidence http://purl.obolibrary.org/obo/ECO_0000226 chromatin immunoprecipitation evidence A type of chromatin immunoprecipitation evidence that uses a tiling microarray for the detection of protein-bound DNA regions where chromatin is immunoprecipitated for protein tagging and DNA is sheared from the cross-linked protein-DNA complex to be arrayed revealing the protein-bound genomic regions. http://purl.obolibrary.org/obo/ECO_0000231 quantitative polymerase chain reaction evidence http://purl.obolibrary.org/obo/ECO_0005519 DNA detection assay evidence A type of DNA detection assay evidence where the reaction product is quantified in real-time across cycles by means of fluorescent dyes or fluorescent probes for detection and quantification of specific sequences in a DNA sample. http://purl.obolibrary.org/obo/ECO_0000232 chromosome conformation-based evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence that is based on a five-step technique of cross-linking DNA in vivo, restriction digestion, intramolecular ligation, reversing DNA cross-links, DNA processing, and quantitation that serves to analyze the spatial organization of chromatin in a cell and quantify the number of interactions between genomic loci that are nearby in 3-D space. http://purl.obolibrary.org/obo/ECO_0000233 3C evidence http://purl.obolibrary.org/obo/ECO_0000232 chromosome conformation-based evidence A type of chromosome conformation-based evidence that is derived by 1) quantifying interactions between a single pair of genomic loci and not multiple interactions and 2) performing polymerase chain reaction (PCR) in the final product quantitation step. http://purl.obolibrary.org/obo/ECO_0000234 4C evidence http://purl.obolibrary.org/obo/ECO_0000232 chromosome conformation-based evidence A type of chromosome conformation-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a prior circularized 3C library followed by product quantitation with a microarray. http://purl.obolibrary.org/obo/ECO_0000235 5C evidence http://purl.obolibrary.org/obo/ECO_0000232 chromosome conformation-based evidence A type of chromosome conformation-based evidence that is derived by performing ligation-mediated amplification (LMA) prior to product quantitation. http://purl.obolibrary.org/obo/ECO_0000237 3C-qPCR evidence http://purl.obolibrary.org/obo/ECO_0000233 3C evidence A type of 3C evidence evidence that 1) quantifies interactions between a single pair of genomic loci (not multiple interactions) and 2) performs quantitative polymerase chain reaction (qPCR) in the quantitation step. http://purl.obolibrary.org/obo/ECO_0000238 Hi-C evidence http://purl.obolibrary.org/obo/ECO_0000232 chromosome conformation-based evidence A type of chromosome conformation-based evidence that is derived by performing immunoprecipitation enrichment of the biotin labelled ligated fragments events prior to sequencing of the library and mapping it to the genome. http://purl.obolibrary.org/obo/ECO_0000239 3C-seq evidence http://purl.obolibrary.org/obo/ECO_0000232 chromosome conformation-based evidence A type of chromosome conformation-based evidence that is derived by inverse polymerase chain reaction (inverse PCR) on a circularized 3C library prior to product quantitation with multiplexed high-throughput (HT) sequencing. http://purl.obolibrary.org/obo/ECO_0000240 anatomical perturbation phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence resulting from the disruption of a structural feature. http://purl.obolibrary.org/obo/ECO_0000241 environmental perturbation phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence resulting from modification to the surroundings or conditions in which an organism lives or operates. http://purl.obolibrary.org/obo/ECO_0000242 tissue ablation phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000240 anatomical perturbation phenotypic evidence A type of anatomical perturbation phenotypic evidence resulting from the surgical removal of tissue or subcellular components. http://purl.obolibrary.org/obo/ECO_0000243 tissue grafting phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000240 anatomical perturbation phenotypic evidence A type of anatomical perturbation phenotypic evidence resulting from the addition of tissue to an organism through a graft procedure. http://purl.obolibrary.org/obo/ECO_0000244 combinatorial evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000352 evidence used in manual assertion A type of combinatorial analysis that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000245 automatically integrated combinatorial evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000244 combinatorial evidence used in manual assertion A type of automatically integrated combinatorial evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000247 sequence alignment evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000250 sequence similarity evidence used in manual assertion A type of sequence alignment evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000248 sequence alignment evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000249 sequence similarity evidence used in automatic assertion A type of sequence alignment evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000249 sequence similarity evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000251 similarity evidence used in automatic assertion A type of sequence similarity evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000250 sequence similarity evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000252 similarity evidence used in manual assertion A type of sequence similarity evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000251 similarity evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000501 evidence used in automatic assertion A type of similarity evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000252 similarity evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000352 evidence used in manual assertion A type of similarity evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000253 genetic similarity evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000252 similarity evidence used in manual assertion A type of genetic similarity evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000254 genetic similarity evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000251 similarity evidence used in automatic assertion A type of genetic similarity evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000255 match to sequence model evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000250 sequence similarity evidence used in manual assertion A type of match to sequence model evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000256 match to sequence model evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000249 sequence similarity evidence used in automatic assertion A type of match to sequence model evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000257 motif similarity evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000255 match to sequence model evidence used in manual assertion A type of motif similarity evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000258 motif similarity evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000256 match to sequence model evidence used in automatic assertion A type of motif similarity evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000259 match to InterPro member signature evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000256 match to sequence model evidence used in automatic assertion A type of match to InterPro member signature evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000260 match to InterPro member signature evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000255 match to sequence model evidence used in manual assertion A type of match to InterPro member signature evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000261 targeting sequence prediction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000258 motif similarity evidence used in automatic assertion A type of targeting sequence prediction that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000262 targeting sequence prediction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000257 motif similarity evidence used in manual assertion A type of targeting sequence prediction that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000263 transmembrane domain prediction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000258 motif similarity evidence used in automatic assertion A type of transmembrane domain prediction that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000264 transmembrane domain prediction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000257 motif similarity evidence used in manual assertion A type of transmembrane domain prediction that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000265 sequence orthology evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000249 sequence similarity evidence used in automatic assertion A type of sequence orthology evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000266 sequence orthology evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000250 sequence similarity evidence used in manual assertion A type of sequence orthology evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000267 enzyme-linked immunoabsorbent assay evidence http://purl.obolibrary.org/obo/ECO_0005517 protein detection assay evidence A type of protein detection assay evidence where an antigen is bound to a substrate, and an antibody directly or indirectly linked to an enzyme is utilized to determine the amount of bound antigen which is visualized with a color change in the substrate. http://purl.obolibrary.org/obo/ECO_0000268 flow cytometry evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence in which cells or particles are characterized by passing them through a light source and monitoring the resultant light scatter or fluorochrome excitation. http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000352 evidence used in manual assertion A type of experimental evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000270 expression pattern evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of expression pattern evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000272 Affymetrix GeneChip evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000276 cRNA to DNA expression microarray evidence used in manual assertion A type of Affymetrix GeneChip evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000273 cDNA to DNA expression microarray evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000275 expression microarray evidence used in manual assertion A type of cDNA to DNA expression microarray evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000274 differential methylation hybridization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001198 in vitro methylation assay evidence used in manual assertion A type of differential methylation hybridization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000275 expression microarray evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000291 transcript expression evidence used in manual assertion A type of expression microarray evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000276 cRNA to DNA expression microarray evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000275 expression microarray evidence used in manual assertion A type of cRNA to DNA expression microarray evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000277 Nimblegen array evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000273 cDNA to DNA expression microarray evidence used in manual assertion A type of evidence arising from a Nimblegen array experiment that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000278 array-based sequence capture evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of array-based sequence capture evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000279 qualitative western immunoblotting evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of qualitative western immunoblotting evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000281 expression library screen evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000284 protein expression evidence used in manual assertion A type of expression library screen evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000282 heterologous protein expression evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000284 protein expression evidence used in manual assertion A type of heterologous protein expression analysis that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000283 spatial pattern of protein expression evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000284 protein expression evidence used in manual assertion A type of protein expression spatial pattern analysis that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000284 protein expression evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000270 expression pattern evidence used in manual assertion A type of protein expression analysis that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000285 DNA to cDNA expression microarray evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000275 expression microarray evidence used in manual assertion A type of DNA to cDNA expression microarray evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000287 differential hybridization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000291 transcript expression evidence used in manual assertion A type of differential hybridization experiment that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000288 RNA protection assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001240 nuclease protection assay evidence used in manual assertion A type of RNA protection assay that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000289 spatial pattern of transcript expression evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000291 transcript expression evidence used in manual assertion A type of spatial pattern of transcript expression analysis that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000290 subtractive hybridization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000291 transcript expression evidence used in manual assertion A type of subtractive hybridization experiment that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000291 transcript expression evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000270 expression pattern evidence used in manual assertion A type of transcript expression evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000292 morpholino experiment evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence that uses anti-sense by introducing morpholino oligonucleotides into the cytosol of a cell. http://purl.obolibrary.org/obo/ECO_0000293 systematic evolution of ligands by exponential amplification evidence http://purl.obolibrary.org/obo/ECO_0000021 physical interaction evidence A type of evidence arising from a physical interaction analysis where a combinatorial chemistry technique is used to identify oligonucleotides that bind to a target ligand. http://purl.obolibrary.org/obo/ECO_0000294 bacterial one-hybrid evidence http://purl.obolibrary.org/obo/ECO_0000025 bait-prey hybrid interaction evidence A type of bait-prey hybrid interaction evidence that uses bacterial transformation with two plasmids to assess in vivo binding of a DNA-binding domain (bait) and DNA target site (prey). http://purl.obolibrary.org/obo/ECO_0000295 RNA-sequencing evidence http://purl.obolibrary.org/obo/ECO_0000009 transcript expression evidence A type of transcript evidence based on high-throughput (HT) sequencing of fragmented cDNA molecules. http://purl.obolibrary.org/obo/ECO_0000298 cleavage arrested development evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence where embryonic development proceeds although cytokinesis is blocked. http://purl.obolibrary.org/obo/ECO_0000299 cytochalasin experiment evidence http://purl.obolibrary.org/obo/ECO_0000298 cleavage arrested development evidence A type of cleavage arrested development that arises after treatment with cytochalasin. http://purl.obolibrary.org/obo/ECO_0000300 green fluorescent protein immunolocalization evidence http://purl.obolibrary.org/obo/ECO_0000087 immunolocalization evidence A type of immunolocalization evidence data that was generated using green fluorescent protein (GFP) as a marker. http://purl.obolibrary.org/obo/ECO_0000301 beta-galactosidase protein immunolocalization evidence http://purl.obolibrary.org/obo/ECO_0000087 immunolocalization evidence A type of immunolocalization evidence that was generated using LacZ protein as a marker. http://purl.obolibrary.org/obo/ECO_0000302 author statement used in manual assertion http://purl.obolibrary.org/obo/ECO_0000204 author statement A type of author statement that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000303 author statement without traceable support used in manual assertion http://purl.obolibrary.org/obo/ECO_0000302 author statement used in manual assertion A type of author statement without traceable support that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000304 author statement supported by traceable reference used in manual assertion http://purl.obolibrary.org/obo/ECO_0000302 author statement used in manual assertion A type of author statement supported by traceable reference that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000305 curator inference used in manual assertion http://purl.obolibrary.org/obo/ECO_0000205 curator inference A type of curator inference that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000306 inference from background scientific knowledge used in manual assertion http://purl.obolibrary.org/obo/ECO_0000305 curator inference used in manual assertion A type of inference from background scientific knowledge that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000307 no evidence data found used in manual assertion http://purl.obolibrary.org/obo/ECO_0000305 curator inference used in manual assertion An inference that results when research finds no evidence information in the scientific literature, at reference databases, or from other resources that is used in an manual assertion. http://purl.obolibrary.org/obo/ECO_0000308 biological aspect of ancestor evidence http://purl.obolibrary.org/obo/ECO_0000080 phylogenetic evidence A type of phylogenetic evidence whereby an aspect of a descendant gene is inferred through the characterization of an aspect of an ancestral gene. http://purl.obolibrary.org/obo/ECO_0000309 cap analysis of gene expression evidence http://purl.obolibrary.org/obo/ECO_0000009 transcript expression evidence A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by a cap-trapper system. http://purl.obolibrary.org/obo/ECO_0000310 nano-cap analysis of gene expression evidence http://purl.obolibrary.org/obo/ECO_0000009 transcript expression evidence A type of transcript expression evidence based on high-throughput (HT) sequencing of the 5' ends of cDNA molecules that are selected by reverse transcriptase template switching. http://purl.obolibrary.org/obo/ECO_0000311 imported information http://purl.obolibrary.org/obo/ECO_0006151 documented statement evidence A type of documented statement evidence that is based on work performed by a person or group prior to a use by a different person or group. http://purl.obolibrary.org/obo/ECO_0000312 imported information used in manual assertion http://purl.obolibrary.org/obo/ECO_0000311 imported information A type of imported information that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000313 imported information used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000311 imported information A type of imported information that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of direct assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000315 mutant phenotype evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000316 genetic interaction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000011 genetic interaction evidence A type of genetic interaction experiment evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000317 genomic context evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000252 similarity evidence used in manual assertion A type of genomic context evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000318 biological aspect of ancestor evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000308 biological aspect of ancestor evidence A type of biological aspect of ancestor evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000319 biological aspect of descendant evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000252 similarity evidence used in manual assertion A type of biological aspect of descendant evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000320 phylogenetic determination of loss of key residues evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000252 similarity evidence used in manual assertion A type of phylogenetic determination of loss of key residues evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000321 rapid divergence from ancestral sequence evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000252 similarity evidence used in manual assertion A type of rapid divergence from ancestral sequence evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000322 imported manually asserted information used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000313 imported information used in automatic assertion Evidence that was initially used in manual assertion by one person or group, which is imported by a second person or group and used in automatic assertion. http://purl.obolibrary.org/obo/ECO_0000323 imported automatically asserted information used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000313 imported information used in automatic assertion A type of imported information used in automatic assertion that was initially used in automatic assertion by one person or group, which is imported by a second person or group and used in automatic assertion. http://purl.obolibrary.org/obo/ECO_0000324 imaging assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence in which an image is created and analyzed. http://purl.obolibrary.org/obo/ECO_0000325 chromatography evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence that is based on separation of constituent parts of a mixture (the mobile phase) as they pass differentially through a stationary phase due to differences in partition coefficient and retention on the stationary phase. http://purl.obolibrary.org/obo/ECO_0000326 transcript splice pattern evidence http://purl.obolibrary.org/obo/ECO_0000200 sequence alignment evidence A type of sequence alignment evidence that is based on comparing the position of exon junctions, relative to a reference genome, for a transcript annotation and a supporting transcript. http://purl.obolibrary.org/obo/ECO_0000327 whole transcript splice pattern evidence http://purl.obolibrary.org/obo/ECO_0000326 transcript splice pattern evidence A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features. http://purl.obolibrary.org/obo/ECO_0000328 coding sequence splice pattern evidence http://purl.obolibrary.org/obo/ECO_0000326 transcript splice pattern evidence A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript. http://purl.obolibrary.org/obo/ECO_0000329 whole transcript splice pattern evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000327 whole transcript splice pattern evidence A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in manual assertion. http://purl.obolibrary.org/obo/ECO_0000330 coding sequence splice pattern evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000328 coding sequence splice pattern evidence A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in manual assertion. http://purl.obolibrary.org/obo/ECO_0000331 coding sequence splice pattern evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000328 coding sequence splice pattern evidence A type of transcript splice pattern evidence that is based on the exon combination of a coding sequence (CDS), a sub-feature of a whole transcript, and is used in automatic assertion. http://purl.obolibrary.org/obo/ECO_0000332 whole transcript splice pattern evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000327 whole transcript splice pattern evidence A type of transcript splice pattern evidence that is based on the exon combination of a whole transcript, rather than its constituent features, and is used in automatic assertion. http://purl.obolibrary.org/obo/ECO_0000333 sodium dodecyl sulfate polyacrylamide gel electrophoresis evidence http://purl.obolibrary.org/obo/ECO_0000337 gel electrophoresis evidence A type of gel electrophoresis evidence where proteins are separated according to their electrophoretic mobility. http://purl.obolibrary.org/obo/ECO_0000334 particle size and count assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence resulting from an assay where both the size of and number of particles in a sample are determined. http://purl.obolibrary.org/obo/ECO_0000335 substance quantification evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where the quantity of a substance used as part of an assay is measured. http://purl.obolibrary.org/obo/ECO_0000336 competitive growth assay evidence http://purl.obolibrary.org/obo/ECO_0005007 cell proliferation assay evidence A type of cell proliferation assay evidence in which a mutated strain of a microorganism, such as a yeast or bacterium, is grown competitively with wild-type cells and the relative fitness of the strains is assessed usually by means of a fluorescent marker and flow cytometric analysis. http://purl.obolibrary.org/obo/ECO_0000337 gel electrophoresis evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where molecules have been sorted according to their size and charge by moving through a gel in the presence of an electric field. http://purl.obolibrary.org/obo/ECO_0000338 pulsed-field gel electrophoresis evidence http://purl.obolibrary.org/obo/ECO_0000337 gel electrophoresis evidence A type of gel electrophoresis evidence in which large DNA molecules are separated on agarose gel by alternately pulsed, perpendicularly oriented electrical fields, at least one of which is inhomogeneous. http://purl.obolibrary.org/obo/ECO_0000339 two-dimensional agarose gel electrophoresis evidence http://purl.obolibrary.org/obo/ECO_0000337 gel electrophoresis evidence A type of gel electrophoresis evidence where DNA molecules are electrophoresed on a low percentage agarose gel followed by high voltage electrophoresis on a higher percentage agarose gel in the presence of ethidium bromide. http://purl.obolibrary.org/obo/ECO_0000340 plasmid maintenance assay evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence in which a plasmid that is capable of being replicated is introduced into cells, cells are grown without selection for several generations, and retention of the plasmid is determined. http://purl.obolibrary.org/obo/ECO_0000341 specific protein inhibition by antibody evidence http://purl.obolibrary.org/obo/ECO_0000020 protein inhibition evidence A type of specific protein inhibition evidence where the molecular function of a protein is inhibited by an antibody. http://purl.obolibrary.org/obo/ECO_0000342 support of intron positions by RNA-sequencing alignment evidence http://purl.obolibrary.org/obo/ECO_0000295 RNA-sequencing evidence A type of RNA-seq evidence where intron locations in predicted transcripts are compared to intron locations supported by RNA-seq evidence. http://purl.obolibrary.org/obo/ECO_0000343 full support of intron positions by RNA-sequencing alignment evidence http://purl.obolibrary.org/obo/ECO_0000342 support of intron positions by RNA-sequencing alignment evidence A type of support of intron positions by RNA-sequencing alignment evidence where RNA-seq alignment from a single sample supports all of the intron positions (exon pairs) predicted for a transcript. http://purl.obolibrary.org/obo/ECO_0000344 partial support of intron positions by RNA-sequencing alignment evidence http://purl.obolibrary.org/obo/ECO_0000342 support of intron positions by RNA-sequencing alignment evidence A type of support of intron positions by RNA-sequencing alignment evidence where RNA-seq alignment to a genome supports only some of the intron positions (exon pairs) predicted for a transcript. http://purl.obolibrary.org/obo/ECO_0000345 single exon transcript confirmation via alignment evidence http://purl.obolibrary.org/obo/ECO_0000200 sequence alignment evidence A type of sequence alignment evidence resulting from the comparison of a single exon transcript to a collection / database of known single exon genes. http://purl.obolibrary.org/obo/ECO_0000346 support of intron positions by RNA-sequencing alignment evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000342 support of intron positions by RNA-sequencing alignment evidence A type of support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000347 support of intron positions by RNA-sequencing alignment evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000342 support of intron positions by RNA-sequencing alignment evidence A type of support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000348 full support of intron positions by RNA-sequencing alignment evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000347 support of intron positions by RNA-sequencing alignment evidence used in automatic assertion A type of full support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000349 full support of intron positions by RNA-sequencing alignment evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000346 support of intron positions by RNA-sequencing alignment evidence used in manual assertion A type of full support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000350 partial support of intron positions by RNA-sequencing alignment evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000347 support of intron positions by RNA-sequencing alignment evidence used in automatic assertion A type of partial support of intron positions by RNA-sequencing alignment evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000351 partial support of intron positions by RNA-sequencing alignment evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000346 support of intron positions by RNA-sequencing alignment evidence used in manual assertion A type of partial support of intron positions by RNA-sequencing alignment evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000352 evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000000 evidence A type of evidence that is used in an manual assertion. http://purl.obolibrary.org/obo/ECO_0000354 gene neighbors evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000317 genomic context evidence used in manual assertion A type of gene neighbors evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0000355 phylogenetic distribution evidence http://purl.obolibrary.org/obo/ECO_0000080 phylogenetic evidence A type of phylogenetic evidence characterized by the mapping of the character states of living organisms onto phylogenies using the method of maximum parsimony. http://purl.obolibrary.org/obo/ECO_0000358 differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) http://purl.obolibrary.org/obo/ECO_0000009 transcript expression evidence A type of transcript expression evidence resulting from a microarray analysis with the use of the Gene Set Enrichment Analysis (GSEA) method or the Fisher's-Exact (FE) method to assess the differential expression. http://purl.obolibrary.org/obo/ECO_0000359 differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) http://purl.obolibrary.org/obo/ECO_0000009 transcript expression evidence A type of transcript expression evidence resulting from the use of the Gene Set Enrichment Analysis (GSEA) method or Fisher's-Exact (FE) method to assess an RNA-seq dataset for differential gene expression. http://purl.obolibrary.org/obo/ECO_0000360 biological target-disease association via drug evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence resulting from the prediction of drug-target interactions by computational means. http://purl.obolibrary.org/obo/ECO_0000361 inferential evidence http://purl.obolibrary.org/obo/ECO_0000000 evidence A type of evidence where an assertion is derived from another assertion via logical inference or some non-logical but rational means. http://purl.obolibrary.org/obo/ECO_0000362 computational inference http://purl.obolibrary.org/obo/ECO_0000361 inferential evidence A type of inferential evidence based on a computationally derived unary inference or an inference chain in which at least one step is computationally derived. http://purl.obolibrary.org/obo/ECO_0000363 computational inference used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000362 computational inference A type of evidence based on computational logical inference that is used in automatic assertion. http://purl.obolibrary.org/obo/ECO_0000364 evidence based on logical inference from manual annotation used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000363 computational inference used in automatic assertion A type of evidence based on logical inference from a manually curated annotation that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000366 evidence based on logical inference from automatic annotation used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000363 computational inference used in automatic assertion A type of evidence based on logical inference from an automatically curated annotation that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0000501 evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000000 evidence A type of evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0001001 3D cell culture evidence http://purl.obolibrary.org/obo/ECO_0005007 cell proliferation assay evidence A type of cell proliferation assay evidence resulting from the growth of cells in a micro-environment that mimics real tissue and establishes physiological cell-cell and cell-substrate interactions that regulate proliferation and differentiation. http://purl.obolibrary.org/obo/ECO_0001002 [3H]arachidonic acid release assay evidence http://purl.obolibrary.org/obo/ECO_0001565 cell-based assay evidence A type of cell-based assay evidence resulting from labeling cells with [3H]arachidonic acid and subsequently determining the release of the arachidonic acid by the cells. http://purl.obolibrary.org/obo/ECO_0001003 [3H]-thymidine incorporation assay evidence http://purl.obolibrary.org/obo/ECO_0005008 DNA synthesis cell proliferation assay evidence A type of DNA synthesis cell proliferation assay evidence resulting from the measurement of cell proliferation rate by determining the incorporation of [3H]-thymidine into cellular nucleic acids. http://purl.obolibrary.org/obo/ECO_0001004 51Cr release assay evidence http://purl.obolibrary.org/obo/ECO_0005012 cytotoxicity assay evidence A type of cytotoxicity assay evidence resulting from the measurement of the amount of radioactive 51Cr released in the supernatant of a sample of 51Cr pre-labeled cells via cytolysis by effector cells (cytotoxic T-cells). http://purl.obolibrary.org/obo/ECO_0001005 7-aminoactinomycin staining evidence http://purl.obolibrary.org/obo/ECO_0005019 staining evidence A type of apoptotic assay evidence resulting from the use of 7-aminoactinomycin D (7-AAD) to distinguish viable, apoptotic, and dead cells, using the fact that permeability of the cell membrane, and fluorescence intensity, is low in early apoptotic cells and high in late apoptotic and dead cells. http://purl.obolibrary.org/obo/ECO_0001006 adhesion assay evidence http://purl.obolibrary.org/obo/ECO_0001565 cell-based assay evidence A type of cell-based assay evidence resulting from the analysis of cell binding either to extracellular matrix proteins or other cells. http://purl.obolibrary.org/obo/ECO_0001007 adoptive cell transfer evidence http://purl.obolibrary.org/obo/ECO_0005502 ex vivo assay evidence A type of ex vivo assay evidence derived by ex vivo selection of tumor-reactive lymphocytes, and their activation and numerical expansion before re-infusion to the autologous tumor-bearing host. http://purl.obolibrary.org/obo/ECO_0001008 alamarBlue assay evidence http://purl.obolibrary.org/obo/ECO_0005004 cell viability assay evidence A type of cell viability assay evidence resulting from the continuous measurement of fluorescence intensity from the viable cells, where a cell permeable compound, resazurin (the active ingredient of alamarBlue), is reduced to fluorescent resorufin. http://purl.obolibrary.org/obo/ECO_0001009 allograft transplantation phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000240 anatomical perturbation phenotypic evidence A type of anatomical perturbation phenotypic evidence resulting from the transplantation of an organ or tissue from one individual of the same species with a different genotype. http://purl.obolibrary.org/obo/ECO_0001010 anion-exchange chromatography evidence http://purl.obolibrary.org/obo/ECO_0000325 chromatography evidence A type of chromatography evidence where a positively charged ion exchange resin with an affinity for molecules having net negative surface charges is used to separate the molecules. http://purl.obolibrary.org/obo/ECO_0001011 annexin-V staining evidence http://purl.obolibrary.org/obo/ECO_0005034 apoptotic assay evidence A type of apoptotic assay evidence resulting from the addition and subsequent binding of Annexin V to phosphatidylserine in cells to detect if they are viable, apoptotic, or necrotic. http://purl.obolibrary.org/obo/ECO_0001012 cognitive assay phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence resulting from behavioral phenotyping and analysis to assess cognitive functioning. http://purl.obolibrary.org/obo/ECO_0001013 blocking monoclonal antibody evidence http://purl.obolibrary.org/obo/ECO_0000040 immunological assay evidence A type of immunological assay evidence resulting from inhibitory effect of monoclonal antibody combining with an antigen over other antibodies. http://purl.obolibrary.org/obo/ECO_0001014 blocking peptide evidence http://purl.obolibrary.org/obo/ECO_0000040 immunological assay evidence A type of immunological assay evidence resulting from the use of peptides to block antibodies from binding to their targets. http://purl.obolibrary.org/obo/ECO_0001015 blocking polyclonal antibody evidence http://purl.obolibrary.org/obo/ECO_0000040 immunological assay evidence A type of immunological assay evidence resulting from the inhibitory effect of polyclonal antibodies (antibodies secreted by different B cell lineages), which while not reacting with a specific antigen, serve to prevent other antibodies from doing so. http://purl.obolibrary.org/obo/ECO_0001016 blood test evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where a blood sample is extracted from an organism to analyze different blood components. http://purl.obolibrary.org/obo/ECO_0001017 Boyden chamber assay evidence http://purl.obolibrary.org/obo/ECO_0005021 chemotaxis assay evidence A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis is determined by measuring the number of cells that migrate from the upper compartment of a chamber (separated by a micro-porous membrane) to the lower compartment, in which chemotactic agents are present. http://purl.obolibrary.org/obo/ECO_0001018 bromodeoxyuridine incorporation assay evidence http://purl.obolibrary.org/obo/ECO_0001107 nucleotide analog incorporation assay evidence A type of nucleotide analog incorporation evidence resulting from the incorporation of bromodeoxyuridine (BrdU) as a thymidine analog into nuclear DNA, resulting in a label that can be tracked using antibody probes. http://purl.obolibrary.org/obo/ECO_0001019 caspase assay evidence http://purl.obolibrary.org/obo/ECO_0005034 apoptotic assay evidence A type of apoptotic assay evidence resulting from the detection of caspase activation, which results in the cleaving of intracellular substrates during apoptosis. http://purl.obolibrary.org/obo/ECO_0001020 cell counting evidence http://purl.obolibrary.org/obo/ECO_0001565 cell-based assay evidence A type of cell-based assay evidence resulting from the quantification of a sample of cells. http://purl.obolibrary.org/obo/ECO_0001021 cell permeability assay evidence http://purl.obolibrary.org/obo/ECO_0001565 cell-based assay evidence A type of cell-based assay evidence resulting from the quantification of cell permeability. http://purl.obolibrary.org/obo/ECO_0001022 carboxyfluorescein diacetate succinimidyl ester staining evidence http://purl.obolibrary.org/obo/ECO_0005019 staining evidence A type of staining evidence resulting from monitoring division by the use of carboxyfluorescein diacetate succinimidyl ester (CFSE) to label intracellular molecules with carboxyfluorescein; when a labeled cell divides, the number of carboxyfluorescein-tagged molecules is split. http://purl.obolibrary.org/obo/ECO_0001023 chemiluminescence-linked immunoassay evidence http://purl.obolibrary.org/obo/ECO_0005517 protein detection assay evidence A type of protein detection assay evidence resulting from a complex (a chemiluminescent compound, protein and a steroidal hapten) utilized as a labeled antigen, which emits light when treated with hydrogen peroxide and copper acetate at a high pH. http://purl.obolibrary.org/obo/ECO_0001024 chimeric protein phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence resulting from the fusion of two or more different genes to create one protein product. http://purl.obolibrary.org/obo/ECO_0001025 co-electrophoresis evidence http://purl.obolibrary.org/obo/ECO_0000337 gel electrophoresis evidence A type of gel electrophoresis evidence resulting from the electrophoresis of two substances together, allowing for the characterization of ligand/nucleic acid binding interactions. http://purl.obolibrary.org/obo/ECO_0001026 co-localization evidence http://purl.obolibrary.org/obo/ECO_0000324 imaging assay evidence A type of imaging assay evidence resulting from the limited observation of the co-occurrence of molecules in a subcellular location. http://purl.obolibrary.org/obo/ECO_0001027 colony counting evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence resulting from the counting of microbial colonies that arise from viable cells grown on a plate or dish. http://purl.obolibrary.org/obo/ECO_0001028 co-sedimentation assay evidence http://purl.obolibrary.org/obo/ECO_0000021 physical interaction evidence A type of physical interaction evidence resulting from the separation of a mixture of molecules under the influence of a force such as artificial gravity, where molecules sedimenting together are assumed to interact. http://purl.obolibrary.org/obo/ECO_0001029 comet assay evidence http://purl.obolibrary.org/obo/ECO_0000337 gel electrophoresis evidence A type of gel electrophoresis evidence resulting in the assessment of DNA breakage in a cell, based on size and shape of DNA migration. http://purl.obolibrary.org/obo/ECO_0001030 conditional knockout evidence http://purl.obolibrary.org/obo/ECO_0001091 knockout phenotypic evidence A type of knockout evidence resulting from a temporally-restricted and/or tissue-specific targeted gene removal. http://purl.obolibrary.org/obo/ECO_0001031 conditional knockin evidence http://purl.obolibrary.org/obo/ECO_0001090 knockin evidence A type of knockin evidence resulting from the targeted introduction of a temporally-restricted and/or tissue-specific mutation. http://purl.obolibrary.org/obo/ECO_0001032 constitutively active mutant protein evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotypic evidence resulting from a mutation causing a change to a protein sequence that produces a constitutively active mutant protein (CAM), that remains active in the absence of upstream signals. http://purl.obolibrary.org/obo/ECO_0001033 cross-linking evidence http://purl.obolibrary.org/obo/ECO_0000024 protein binding evidence A type of protein binding evidence resulting from the identification of two interacting proteins (that exist in close proximity) by linking through covalent bonds followed by identification http://purl.obolibrary.org/obo/ECO_0001034 crystallography evidence http://purl.obolibrary.org/obo/ECO_0005031 structure determination evidence A type of structure determination evidence resulting from the use of a narrow beam of x-rays to identify molecular structures by creating a diffraction pattern. http://purl.obolibrary.org/obo/ECO_0001035 cytochemistry evidence http://purl.obolibrary.org/obo/ECO_0001050 histochemistry evidence A type of histochemistry evidence resulting from localizing chemical components of cells and organelles on histological sections. http://purl.obolibrary.org/obo/ECO_0001036 cytochrome C release assay evidence http://purl.obolibrary.org/obo/ECO_0005034 apoptotic assay evidence A type of apoptotic assay evidence resulting from the identification of an accumulation of cytochrome C in the cytoplasm after its release from the mitochondria, an early event in apoptosis. http://purl.obolibrary.org/obo/ECO_0001037 4',6-diamidino-2-phenylindole staining evidence http://purl.obolibrary.org/obo/ECO_0005019 staining evidence A type of staining evidence resulting from 4',6-diamidino-2-phenylindole (DAPI) binding to AT regions of DNA and emitting a blue fluorescence. http://purl.obolibrary.org/obo/ECO_0001038 deletion mutation phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence resulting from a mutation in which there is the removal of one or more contiguous nucleotides that may result in altered function or other measurable properties. http://purl.obolibrary.org/obo/ECO_0001039 DNA laddering assay evidence http://purl.obolibrary.org/obo/ECO_0005034 apoptotic assay evidence A type of apoptotic assay evidence resulting from the visualization via gel electrophoresis of the fragmented DNA (DNA ladder) which is the result of apoptotic DNA fragmentation. http://purl.obolibrary.org/obo/ECO_0001040 RNA dot blot assay evidence http://purl.obolibrary.org/obo/ECO_0005518 RNA detection assay evidence A type of RNA detection assay evidence based on the direct application of a RNA mixture in a circular motion on a matrix for hybridization with labeled DNA fragments. http://purl.obolibrary.org/obo/ECO_0001042 dominant-negative mutant phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence resulting from a mutated gene producing mutant polypeptides that disrupt the activity of the wild-type genes. http://purl.obolibrary.org/obo/ECO_0001043 Edman degradation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001044 Edman degradation evidence A type of Edman degradation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001044 Edman degradation evidence http://purl.obolibrary.org/obo/ECO_0005517 protein detection assay evidence A type of protein detection assay evidence resulting from labeling a termonal amino acid residue and cleaving it from a peptide by sequentially removing one residue at a time from the amino end of the peptide, without disrupting the bonds. http://purl.obolibrary.org/obo/ECO_0001045 eTag assay evidence http://purl.obolibrary.org/obo/ECO_0000023 affinity evidence A type of affinity evidence resulting from the quantitative analysis of gene and protein expression monitored by proprietary eTag reporters. http://purl.obolibrary.org/obo/ECO_0001046 filter binding assay evidence http://purl.obolibrary.org/obo/ECO_0000021 physical interaction evidence A type of physical interaction evidence resulting from a mixture of two molecules passed through a nitrocellulose filter, where one may be immobilized on the filter, and if the immobilized molecule is capable of binding to the other, it will be retained on the filter as well. http://purl.obolibrary.org/obo/ECO_0001047 fluorescence in situ hybridization evidence http://purl.obolibrary.org/obo/ECO_0001836 in situ hybridization evidence A type of nucleic acid localization evidence and in situ hybridization evidence resulting from the use of fluorescent probes to detect complementary sequences of nucleic acids. http://purl.obolibrary.org/obo/ECO_0001048 fluorescence resonance energy transfer evidence http://purl.obolibrary.org/obo/ECO_0006273 dynamic fluorescence quenching evidence A type of dynamic fluorescence quenching evidence resulting from the measurement of the proximity of two fluorophores, where the energy from an excited molecular fluorophore to another fluorophore can only occur within ~10nm. http://purl.obolibrary.org/obo/ECO_0001049 gel-filtration evidence http://purl.obolibrary.org/obo/ECO_0000325 chromatography evidence A type of chromatography evidence resulting from the exclusion of proteins based on molecular size by filtration through a porous matrix (swollen gel), from which larger molecules are excluded. http://purl.obolibrary.org/obo/ECO_0001050 histochemistry evidence http://purl.obolibrary.org/obo/ECO_0001051 histology evidence A type of histology evidence resulting from the identification of chemical components in cells and tissues. http://purl.obolibrary.org/obo/ECO_0001051 histology evidence http://purl.obolibrary.org/obo/ECO_0000324 imaging assay evidence A type of imaging assay evidence resulting from the qualitative microscopic examination of cells or tissues. http://purl.obolibrary.org/obo/ECO_0001052 high-performance liquid chromatography evidence http://purl.obolibrary.org/obo/ECO_0000325 chromatography evidence A type of chromatography evidence resulting from the separation of molecules in a solute by forcing the solvent containing the sample through a column packed with nonporous particles at high pressure. http://purl.obolibrary.org/obo/ECO_0001053 immunocytochemistry evidence http://purl.obolibrary.org/obo/ECO_0005517 protein detection assay evidence A type of protein detection assay evidence resulting from the use of antibodies linked to coloring agents to localize structures in cell cultures to identify proteins. http://purl.obolibrary.org/obo/ECO_0001054 immunodepletion evidence http://purl.obolibrary.org/obo/ECO_0000040 immunological assay evidence A type of immunological assay evidence resulting from the use of antibodies to remove specific proteins from a sample. http://purl.obolibrary.org/obo/ECO_0001055 immunohistochemistry evidence http://purl.obolibrary.org/obo/ECO_0005517 protein detection assay evidence A type of protein detection assay evidence resulting from the use of antibodies to detect proteins in localized cells of tissue sections. http://purl.obolibrary.org/obo/ECO_0001057 in vitro acetylation assay evidence http://purl.obolibrary.org/obo/ECO_0001546 acetylation assay evidence A type of acetylation assay evidence used in an in vitro experiment. http://purl.obolibrary.org/obo/ECO_0001058 in vitro cleavage assay evidence http://purl.obolibrary.org/obo/ECO_0001547 cleavage assay evidence A type of cleavage assay evidence used in an in vitro experiment. http://purl.obolibrary.org/obo/ECO_0001059 in vitro deubiquitination assay evidence http://purl.obolibrary.org/obo/ECO_0001552 deubiquitination assay evidence A type of deubiquitination assay evidence used in an in vitro experiment. http://purl.obolibrary.org/obo/ECO_0001060 in vitro deacetylation assay evidence http://purl.obolibrary.org/obo/ECO_0001548 deacetylation assay evidence A type of deacetylation assay evidence used in an in vitro experiment. http://purl.obolibrary.org/obo/ECO_0001061 in vitro defarnesylation assay evidence http://purl.obolibrary.org/obo/ECO_0001549 defarnesylation assay evidence A type of defarnesylation assay evidence used in an in vitro experiment. http://purl.obolibrary.org/obo/ECO_0001062 in vitro demethylation assay evidence http://purl.obolibrary.org/obo/ECO_0001550 demethylation assay evidence A type of demethylation assay evidence used in an in vitro experiment. http://purl.obolibrary.org/obo/ECO_0001063 in vitro desumoylation assay evidence http://purl.obolibrary.org/obo/ECO_0001551 desumoylation assay evidence A type of desumoylation assay evidence used in an in vitro experiment. http://purl.obolibrary.org/obo/ECO_0001064 in vitro farnesylation assay evidence http://purl.obolibrary.org/obo/ECO_0001553 farnesylation assay evidence A type of farnesylation assay evidence used in an in vitro experiment. http://purl.obolibrary.org/obo/ECO_0001065 in vitro methylation assay evidence http://purl.obolibrary.org/obo/ECO_0001554 methylation assay evidence A type of methylation assay evidence used in an in vitro experiment. http://purl.obolibrary.org/obo/ECO_0001066 in vitro palmitoylation assay evidence http://purl.obolibrary.org/obo/ECO_0001555 palmitoylation assay evidence A type of palmitoylation assay evidence used in an in vitro experiment. http://purl.obolibrary.org/obo/ECO_0001067 in vitro phosphatase assay evidence http://purl.obolibrary.org/obo/ECO_0001556 phosphatase assay evidence A type of phosphatase assay evidence used in an in vitro experiment. http://purl.obolibrary.org/obo/ECO_0001068 in vitro protein kinase assay evidence http://purl.obolibrary.org/obo/ECO_0001558 protein kinase assay evidence A type of protein kinase assay evidence used in an in vitro experiment. http://purl.obolibrary.org/obo/ECO_0001069 in vitro polyADP-ribosylation assay evidence http://purl.obolibrary.org/obo/ECO_0001557 polyADP-ribosylation assay evidence A type of polyADP-ribosylation assay evidence used in an in vitro experiment. http://purl.obolibrary.org/obo/ECO_0001070 in vitro sumoylation assay evidence http://purl.obolibrary.org/obo/ECO_0001559 sumoylation assay evidence A type of sumoylation assay evidence used in an in vitro experiment. http://purl.obolibrary.org/obo/ECO_0001071 in vitro transcription assay evidence http://purl.obolibrary.org/obo/ECO_0000168 transcription assay evidence A type of transcription assay evidence used in an in vitro experiment. http://purl.obolibrary.org/obo/ECO_0001072 in vitro translation assay evidence http://purl.obolibrary.org/obo/ECO_0001561 translation assay evidence A type of translation assay evidence used in an in vitro experiment. http://purl.obolibrary.org/obo/ECO_0001073 in vitro ubiquitination assay evidence http://purl.obolibrary.org/obo/ECO_0001562 ubiquitination assay evidence A type of ubiquitination assay evidenceused in an in vitro experiment. http://purl.obolibrary.org/obo/ECO_0001074 in vivo acetylation assay evidence http://purl.obolibrary.org/obo/ECO_0001546 acetylation assay evidence A type of acetylation assay evidence used in an in vivo experiment. http://purl.obolibrary.org/obo/ECO_0001075 in vivo cleavage assay evidence http://purl.obolibrary.org/obo/ECO_0001547 cleavage assay evidence A type of cleavage assay evidence used in an in vivo experiment. http://purl.obolibrary.org/obo/ECO_0001076 in vivo deacetylation assay evidence http://purl.obolibrary.org/obo/ECO_0001548 deacetylation assay evidence A type of deacetylation assay evidence used in an in vivo experiment. http://purl.obolibrary.org/obo/ECO_0001077 in vivo defarnesylation assay evidence http://purl.obolibrary.org/obo/ECO_0001549 defarnesylation assay evidence A type of defarnesylation assay evidence used in an in vivo experiment. http://purl.obolibrary.org/obo/ECO_0001078 in vivo demethylation assay evidence http://purl.obolibrary.org/obo/ECO_0001550 demethylation assay evidence A type of demethylation assay evidence used in an in vivo experiment. http://purl.obolibrary.org/obo/ECO_0001079 in vivo deubiquitination assay evidence http://purl.obolibrary.org/obo/ECO_0001552 deubiquitination assay evidence A type of deubiquitination assay evidence used in an in vivo experiment. http://purl.obolibrary.org/obo/ECO_0001080 in vivo desumoylation assay evidence http://purl.obolibrary.org/obo/ECO_0001551 desumoylation assay evidence A type of desumoylation assay evidence used in an in vivo experiment. http://purl.obolibrary.org/obo/ECO_0001081 in vivo farnesylation assay evidence http://purl.obolibrary.org/obo/ECO_0001553 farnesylation assay evidence A type of farnesylation assay evidence used in an in vivo experiment. http://purl.obolibrary.org/obo/ECO_0001082 in vivo methylation assay evidence http://purl.obolibrary.org/obo/ECO_0001554 methylation assay evidence A type of methylation assay evidence used in an in vivo experiment. http://purl.obolibrary.org/obo/ECO_0001083 in vivo palmitoylation assay evidence http://purl.obolibrary.org/obo/ECO_0001555 palmitoylation assay evidence A type of palmitoylation assay evidence used in an in vivo experiment. http://purl.obolibrary.org/obo/ECO_0001084 in vivo phosphatase assay evidence http://purl.obolibrary.org/obo/ECO_0001556 phosphatase assay evidence A type of phosphatase assay evidence used in an in vivo experiment. http://purl.obolibrary.org/obo/ECO_0001085 in vivo protein kinase assay evidence http://purl.obolibrary.org/obo/ECO_0001558 protein kinase assay evidence A type of protein kinase assay evidence used in an in vivo experiment. http://purl.obolibrary.org/obo/ECO_0001086 in vivo sumoylation assay evidence http://purl.obolibrary.org/obo/ECO_0001559 sumoylation assay evidence A type of sumoylation assay evidence used in an in vivo experiment. http://purl.obolibrary.org/obo/ECO_0001087 in vivo transcription assay evidence http://purl.obolibrary.org/obo/ECO_0000168 transcription assay evidence A type of transcription assay evidence used in an in vivo experiment. http://purl.obolibrary.org/obo/ECO_0001088 in vivo translation assay evidence http://purl.obolibrary.org/obo/ECO_0001561 translation assay evidence A type of translation assay evidence used in an in vivo experiment. http://purl.obolibrary.org/obo/ECO_0001089 in vivo ubiquitination assay evidence http://purl.obolibrary.org/obo/ECO_0001562 ubiquitination assay evidence A type of ubiquitination assay evidence used in an in vivo experiment. http://purl.obolibrary.org/obo/ECO_0001090 knockin evidence http://purl.obolibrary.org/obo/ECO_0005027 genetic transformation evidence A type of genetic transformation evidence resulting from the targetted replacement of a wild-type DNA sequence with a different sequence. http://purl.obolibrary.org/obo/ECO_0001091 knockout phenotypic evidence http://purl.obolibrary.org/obo/ECO_0005027 genetic transformation evidence A type of genetic transformation evidence resulting from a change in the DNA that results in the elimination of the function of the gene, allowing for functional analysis. http://purl.obolibrary.org/obo/ECO_0001092 lipid binding assay evidence http://purl.obolibrary.org/obo/ECO_0000021 physical interaction evidence A type of physical interaction evidence resulting from the qualitative and quantitative analysis of the affinity with which a protein binds to a lipid. http://purl.obolibrary.org/obo/ECO_0001093 luminescence-based mammalian interactome mapping assay evidence http://purl.obolibrary.org/obo/ECO_0000021 physical interaction evidence A type of physical interaction evidence resulting from the analysis of protein-protein interactions by use of a luciferase enzyme fused to a particular protein (or proteins), which are coexpressed with epitope-tagged partners in mammalian cells. http://purl.obolibrary.org/obo/ECO_0001094 macroscopy evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence that is visible to the naked eye cf. microscopy evidence which requires the aid of a microscope. http://purl.obolibrary.org/obo/ECO_0001095 mammalian 2-hybrid assay evidence http://purl.obolibrary.org/obo/ECO_0000025 bait-prey hybrid interaction evidence A type of bait-prey hybrid interaction evidence resulting from the analysis of proteins of interest attached to two portions of the transcriptional activator, and in interaction bring the two portions together to increase expression of the reporter gene. http://purl.obolibrary.org/obo/ECO_0001096 mass spectrometry evidence http://purl.obolibrary.org/obo/ECO_0005504 spectrometry evidence A type of spectrometry evidence resulting from identifying the amount and type of material entities present in a sample by fragmenting it and measuring the mass-to-charge ratio of the resulting particles. http://purl.obolibrary.org/obo/ECO_0001097 medical imaging evidence http://purl.obolibrary.org/obo/ECO_0000324 imaging assay evidence A type of imaging assay evidence resulting from the use of technology to visualize and provide information about the body to diagnose, treat, or monitor medical conditions. http://purl.obolibrary.org/obo/ECO_0001098 microscopy evidence http://purl.obolibrary.org/obo/ECO_0000324 imaging assay evidence A type of direct assay evidence resulting from the use of a microscope. http://purl.obolibrary.org/obo/ECO_0001099 motility wound healing assay evidence http://purl.obolibrary.org/obo/ECO_0001565 cell-based assay evidence A type of cell-based assay evidence to measure cell motility in which a "wound" is created in a cell monolayer and the migration of the cells is captured by imaging at regular intervals during cell migration to close the wound. http://purl.obolibrary.org/obo/ECO_0001100 MTS assay evidence http://purl.obolibrary.org/obo/ECO_0005034 apoptotic assay evidence A type of apoptotic assay evidence resulting from the reduction of 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) in combination with phenazine methyl sulfate (PMS) as an intermediate electron acceptor reagent to produce a soluble formazan dye in viable cells. http://purl.obolibrary.org/obo/ECO_0001101 MTT assay evidence http://purl.obolibrary.org/obo/ECO_0005034 apoptotic assay evidence A type of apoptotic assay evidence resulting from the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to its insoluble purple formazan dye in viable cells and measures cell viability / metabolic activity. http://purl.obolibrary.org/obo/ECO_0001102 multiplex bead-based immunoassay evidence http://purl.obolibrary.org/obo/ECO_0005517 protein detection assay evidence A type of protein detection assay evidence resulting from determination of analyte concentration by fluorescent bead sets attached to an antibody (to a specific analyte) with a fluorescent reporter dye label attached to a second antibody (to a specific analyte). http://purl.obolibrary.org/obo/ECO_0001103 natural variation mutant evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence resulting from the observation of the impact of a natural mutation on the expressed traits. http://purl.obolibrary.org/obo/ECO_0001104 nuclear fragmentation evidence http://purl.obolibrary.org/obo/ECO_0005034 apoptotic assay evidence A type of apoptotic assay evidence resulting from the nuclei fragmenting into smaller pieces during programmed cell death. http://purl.obolibrary.org/obo/ECO_0001105 nuclear magnetic resonance evidence http://purl.obolibrary.org/obo/ECO_0006252 magnetic resonance evidence A type of magnetic resonance evidence in which atomic nuclei in a strong constant magnetic field are perturbed by a weak oscillating magnetic field and the resulting electromagnetic signal is captured. http://purl.obolibrary.org/obo/ECO_0001106 nuclease protection assay evidence http://purl.obolibrary.org/obo/ECO_0005518 RNA detection assay evidence A type of RNA detection assay evidence resulting from the detection and quantitation of specific RNAs (from a sample of total cellular RNA) that are bound by antisense RNA or DNA probes and therefore protected from nucleases. http://purl.obolibrary.org/obo/ECO_0001107 nucleotide analog incorporation assay evidence http://purl.obolibrary.org/obo/ECO_0005008 DNA synthesis cell proliferation assay evidence A type of DNA synthesis cell proliferation assay evidence resulting from the analysis and measurement of the incorporation of fluorescently labeled nucleotide analog(s) into the DNA. http://purl.obolibrary.org/obo/ECO_0001108 phage display evidence http://purl.obolibrary.org/obo/ECO_0000024 protein binding evidence A type of protein binding evidence resulting from the panning of a phage library where the individual phage displayed a different peptide or protein by fusion to coat proteins on the capsid. http://purl.obolibrary.org/obo/ECO_0001109 phosphoamino acid analysis evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence resulting from the identification of the phosphorylated residue in a protein by amino acid analysis. http://purl.obolibrary.org/obo/ECO_0001110 peptide affinity enrichment evidence http://purl.obolibrary.org/obo/ECO_0000023 affinity evidence A type of affinity evidence resulting from the appending of affinity tags on proteins so that they may be purified from their biological source using an affinity technique. http://purl.obolibrary.org/obo/ECO_0001111 physical examination evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence resulting from the physical examination and measurement of the feature of a subject or sample. http://purl.obolibrary.org/obo/ECO_0001112 peptide array evidence http://purl.obolibrary.org/obo/ECO_0000024 protein binding evidence A type of protein binding evidence resulting from protein-bound peptides detected from a collection of peptides arranged as an array and incubated with the partner protein in order to map protein-protein interaction sites. http://purl.obolibrary.org/obo/ECO_0001113 point mutation phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence resulting from the change in a single nucleotide. http://purl.obolibrary.org/obo/ECO_0001114 propidium iodide staining evidence http://purl.obolibrary.org/obo/ECO_0005019 staining evidence A type of staining evidence resulting from the binding and labeling of DNA (during apoptosis, when there is a loss of nuclear DNA content) with propidium iodide (PI) to identify the cells from which it originated. http://purl.obolibrary.org/obo/ECO_0001115 fluorescence evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence resulting from the analysis of a molecule by its intrinsic fluorescence, or by attaching it with a fluorophore. http://purl.obolibrary.org/obo/ECO_0001116 protein dot blot assay evidence http://purl.obolibrary.org/obo/ECO_0005517 protein detection assay evidence A type of protein detection assay evidence resulting from protein samples immobilized on a membrane, then subsequently incubated and imaged. http://purl.obolibrary.org/obo/ECO_0001117 protein microarray evidence http://purl.obolibrary.org/obo/ECO_0005517 protein detection assay evidence A type of protein detection assay evidence resulting from proteins immobilized to prepare protein chips, or microwells, that can be utilized to determine presence of proteins, monitor differential expression profiles, and/or study protein interactions. http://purl.obolibrary.org/obo/ECO_0001118 protein sequencing assay evidence http://purl.obolibrary.org/obo/ECO_0000220 sequencing assay evidence A type of sequencing assay evidence resulting from determining the sequence of amino acids in a protein. http://purl.obolibrary.org/obo/ECO_0001119 quantitative mass spectrometry evidence http://purl.obolibrary.org/obo/ECO_0001096 mass spectrometry evidence A type of mass spectrometry evidence resulting from quantitative analysis of peptides, proteins, and proteomes. http://purl.obolibrary.org/obo/ECO_0001120 radioisotope assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence resulting from the assessment of biological reactions by use of a radioactive isotope to label the reactant. http://purl.obolibrary.org/obo/ECO_0001121 radioimmunoassay evidence http://purl.obolibrary.org/obo/ECO_0005517 protein detection assay evidence A type of protein detection assay evidence resulting from the use of a radioligand to measure the binding of a substance to a specific antibody or other receptor system. http://purl.obolibrary.org/obo/ECO_0001123 resonant mirror biosensor evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence from the analysis of the real-time interaction between an analyte in solution and its interactant linked to the surface of the resonant mirror. http://purl.obolibrary.org/obo/ECO_0001124 restriction fragment detection evidence http://purl.obolibrary.org/obo/ECO_0005519 DNA detection assay evidence A type of DNA detection assay evidence resulting from homologous DNA fragments digested by restriction enzymes, and the resulting restriction fragments are sorted by length to illustrate differences. http://purl.obolibrary.org/obo/ECO_0001126 spectrophotometry evidence http://purl.obolibrary.org/obo/ECO_0005504 spectrometry evidence A type of spectrometry evidence resulting from the evaluation of a molecule in a fluid or solid by its ability to alter the transmission of light at specific wavelengths. http://purl.obolibrary.org/obo/ECO_0001127 surface plasmon resonance evidence http://purl.obolibrary.org/obo/ECO_0000021 physical interaction evidence A type of physical interaction evidence based on real-time, rapid plasmon generation on the interface between a planar surface and vacuum that measures changes in refractive index close to the sensor surface when an analyte and its immobilized ligand bind to observe and characterize molecular interaction. http://purl.obolibrary.org/obo/ECO_0001128 syngeneic transplantation experiment evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence resulting from a transplantation in which the transplanted material (stem cells) is from an individual's identical twin. http://purl.obolibrary.org/obo/ECO_0001129 TACE activity assay evidence http://purl.obolibrary.org/obo/ECO_0000005 enzymatic activity assay evidence A type of enzymatic activity assay evidence resulting from the analysis of tumor necrosis factor (TNF)-a converting enzyme (TACE), which releases a soluble TNF-a from the membrane-bound precursor protein. http://purl.obolibrary.org/obo/ECO_0001130 tissue microarray evidence http://purl.obolibrary.org/obo/ECO_0001035 cytochemistry evidence A type of cytochemistry evidence resulting from the analysis of section(s) of a paraffin block containing embedded tissues cores arranged in an array pattern. http://purl.obolibrary.org/obo/ECO_0001131 transgenic organism evidence http://purl.obolibrary.org/obo/ECO_0005027 genetic transformation evidence A type of genetic transformation evidence resulting from an organism that has had its expressed phenotype altered by modification. http://purl.obolibrary.org/obo/ECO_0001132 tryptic phosphopeptide mapping assay evidence http://purl.obolibrary.org/obo/ECO_0001096 mass spectrometry evidence A type of mass spectrometry evidence resulting from the analysis of prepared phosphopeptides separated in two dimensions on a TLC plate. http://purl.obolibrary.org/obo/ECO_0001133 terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence http://purl.obolibrary.org/obo/ECO_0005034 apoptotic assay evidence A type of apoptotic assay evidence resulting from the visualization of DNA fragmentation by detection of exposed 3'-OH ends, localized by terminal deoxynucleotidyl transferase (TdT) which then catalyzes the addition of labeled dUTPs. http://purl.obolibrary.org/obo/ECO_0001134 urine test evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence resulting from the analysis of a urine sample. http://purl.obolibrary.org/obo/ECO_0001136 WST-1 assay evidence http://purl.obolibrary.org/obo/ECO_0005007 cell proliferation assay evidence A type of cell proliferation assay evidence resulting from the assessment of metabolic activity from a water-soluble tetrazolium salt WST-1 being reduced outside the cell (by reacting with mitochondrial succinate-tetrazolium reductase) to form a formazan dye. http://purl.obolibrary.org/obo/ECO_0001137 xenotransplantation phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000240 anatomical perturbation phenotypic evidence A type of anatomical perturbation phenotypic evidence resulting from the transplantation, implantation, or infusion of live cells, tissues, or organs between individuals of different species. http://purl.obolibrary.org/obo/ECO_0001138 3D cell culture evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001001 3D cell culture evidence A type of 3D cell culture evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001139 51Cr release assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001004 51Cr release assay evidence A type of 51Cr release assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001140 7-aminoactinomycin staining evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001005 7-aminoactinomycin staining evidence A type of 7-aminoactinomycin staining evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001141 [3H]-thymidine incorporation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001003 [3H]-thymidine incorporation assay evidence A type of [3H]-thymidine incorporation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001142 [3H]arachidonic acid release assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001002 [3H]arachidonic acid release assay evidence A type of [3H]arachidonic acid release assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001143 adhesion assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001006 adhesion assay evidence A type of adhesion assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001144 adoptive cell transfer evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001007 adoptive cell transfer evidence A type of adoptive cell transfer evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001145 alamarBlue assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001008 alamarBlue assay evidence A type of alamarBlue assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001146 allograft transplantation phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001009 allograft transplantation phenotypic evidence A type of allograft transplantation phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001147 anion-exchange chromatography evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007680 chromatography evidence used in manual assertion A type of anion-exchange chromatography evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001148 annexin-V staining evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001011 annexin-V staining evidence A type of annexin-V staining evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001149 cognitive assay phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001012 cognitive assay phenotypic evidence A type of cognitive assay phenotypic evidence used that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001150 blocking monoclonal antibody evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001013 blocking monoclonal antibody evidence A type of blocking monoclonal antibody evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001151 blocking peptide evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001014 blocking peptide evidence A type of blocking peptide evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001152 blocking polyclonal antibody evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001015 blocking polyclonal antibody evidence A type of blocking polyclonal antibody evidence tthat is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001153 blood test evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001016 blood test evidence A type of blood test evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001154 Boyden chamber assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001017 Boyden chamber assay evidence A type of Boyden chamber assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001155 bromodeoxyuridine incorporation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001241 nucleotide analog incorporation assay evidence used in manual assertion A type of bromodeoxyuridine incorporation assay evidence used in manual assertion. http://purl.obolibrary.org/obo/ECO_0001156 caspase assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001019 caspase assay evidence A type of caspase assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001157 cell counting evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001020 cell counting evidence A type of cell counting evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001158 cell permeability assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001021 cell permeability assay evidence A type of cell permeability assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001159 carboxyfluorescein diacetate succinimidyl ester staining evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001022 carboxyfluorescein diacetate succinimidyl ester staining evidence A type of carboxyfluorescein diacetate succinimidyl ester staining evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001160 chemiluminescence-linked immunoassay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001023 chemiluminescence-linked immunoassay evidence A type of chemiluminescence-linked immunoassay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001161 chimeric protein phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001024 chimeric protein phenotypic evidence A type of chimeric protein phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001162 co-electrophoresis evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001025 co-electrophoresis evidence A type of co-electrophoresis evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001163 co-localization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001256 imaging assay evidence used in manual assertion A type of co-localization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001164 co-sedimentation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001028 co-sedimentation assay evidence A type of co-sedimentation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001165 colony counting evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001027 colony counting evidence A type of colony counting evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001166 comet assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001029 comet assay evidence A type of comet assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001167 conditional knockin evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001224 knockin evidence used in manual assertion A type of conditional knockin evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001168 conditional knockout evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001225 knockout evidence used in manual assertion A type of conditional knockout evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001169 constitutively active mutant protein evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001032 constitutively active mutant protein evidence A type of constitutively active mutant protein evidence that is used a in manual assertion. http://purl.obolibrary.org/obo/ECO_0001170 cross-linking evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001033 cross-linking evidence A type of cross-linking evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001171 crystallography evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001034 crystallography evidence A type of crystallography evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001172 cytochemistry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001185 histochemistry evidence used in manual assertion A type of cytochemistry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001173 cytochrome C release assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001036 cytochrome C release assay evidence A type of cytochrome C release assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001174 4',6-diamidino-2-phenylindole staining evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001037 4',6-diamidino-2-phenylindole staining evidence A type of 4',6-diamidino-2-phenylindole staining evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001175 deletion mutation phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001038 deletion mutation phenotypic evidence A type of deletion mutation phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001176 DNA laddering assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001039 DNA laddering assay evidence A type of DNA laddering assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001177 RNA dot blot assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001040 RNA dot blot assay evidence A type of RNA dot blot assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001179 dominant-negative mutant phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001042 dominant-negative mutant phenotypic evidence A type of dominant-negative mutant phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001180 eTag assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001045 eTag assay evidence A type of eTag assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001181 filter binding assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001046 filter binding assay evidence A type of filter binding assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001182 fluorescence in situ hybridization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001838 in situ hybridization evidence used in manual assertion A type of fluorescence in situ hybridization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001183 fluorescence resonance energy transfer evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001048 fluorescence resonance energy transfer evidence A type of fluorescence resonance energy transfer evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001184 gel-filtration evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007680 chromatography evidence used in manual assertion A type of gel-filtration evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001185 histochemistry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001187 histology evidence used in manual assertion A type of histochemistry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001186 immunocytochemistry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001053 immunocytochemistry evidence A type of immunocytochemistry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001187 histology evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001256 imaging assay evidence used in manual assertion A type of histology evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001188 immunodepletion evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001054 immunodepletion evidence A type of immunodepletion evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001189 immunohistochemistry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001055 immunohistochemistry evidence A type of immunohistochemistry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001190 in vitro acetylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001057 in vitro acetylation assay evidence A type of in vitro acetylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001191 in vitro cleavage assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001058 in vitro cleavage assay evidence A type of in vitro cleavage assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001192 in vitro deacetylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001060 in vitro deacetylation assay evidence A type of in vitro deacetylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001193 in vitro defarnesylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001061 in vitro defarnesylation assay evidence A type of in vitro defarnesylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001194 in vitro demethylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001062 in vitro demethylation assay evidence A type of in vitro demethylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001195 in vitro desumoylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001063 in vitro desumoylation assay evidence A type of in vitro desumoylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001196 in vitro deubiquitination assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001059 in vitro deubiquitination assay evidence A type of in vitro deubiquitination assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001197 in vitro farnesylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001064 in vitro farnesylation assay evidence A type of in vitro farnesylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001198 in vitro methylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001065 in vitro methylation assay evidence A type of in vitro methylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001199 in vitro palmitoylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001066 in vitro palmitoylation assay evidence A type of in vitro palmitoylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001200 in vitro phosphatase assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001067 in vitro phosphatase assay evidence A type of in vitro phosphatase assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001201 in vitro polyADP-ribosylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001069 in vitro polyADP-ribosylation assay evidence A type of in vitro polyADP-ribosylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001202 in vitro protein kinase assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005801 enzymatic activity assay evidence used in manual assertion A type of in vitro protein kinase assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001203 in vitro sumoylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001070 in vitro sumoylation assay evidence A type of in vitro sumoylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001204 in vitro transcription assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001071 in vitro transcription assay evidence A type of in vitro transcription assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001205 in vitro translation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001072 in vitro translation assay evidence A type of in vitro translation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001206 in vitro ubiquitination assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001073 in vitro ubiquitination assay evidence A type of in vitro ubiquitination assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001207 in vivo acetylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001074 in vivo acetylation assay evidence A type of in vivo acetylation assay evidence that is used in manual assertion. http://purl.obolibrary.org/obo/ECO_0001208 in vivo cleavage assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001075 in vivo cleavage assay evidence A type of in vivo cleavage assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001209 in vivo deacetylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001076 in vivo deacetylation assay evidence A type of in vivo deacetylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001210 in vivo defarnesylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001077 in vivo defarnesylation assay evidence A type of in vivo defarnesylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001211 in vivo demethylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001078 in vivo demethylation assay evidence A type of in vivo demethylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001212 in vivo desumoylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001080 in vivo desumoylation assay evidence A type of in vivo desumoylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001213 in vivo deubiquitination assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001079 in vivo deubiquitination assay evidence A type of in vivo deubiquitination assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001214 in vivo farnesylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001081 in vivo farnesylation assay evidence A type of in vivo farnesylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001215 in vivo methylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001082 in vivo methylation assay evidence A type of in vivo methylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001216 in vivo palmitoylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001083 in vivo palmitoylation assay evidence A type of in vivo palmitoylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001217 in vivo phosphatase assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001084 in vivo phosphatase assay evidence A type of in vivo phosphatase assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001218 in vivo protein kinase assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005801 enzymatic activity assay evidence used in manual assertion A type of in vivo protein kinase assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001219 in vivo sumoylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001086 in vivo sumoylation assay evidence A type of in vivo sumoylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001220 in vivo transcription assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001087 in vivo transcription assay evidence A type of in vivo transcription assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001221 in vivo translation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001088 in vivo translation assay evidence A type of in vivo translation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001222 in vivo ubiquitination assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001089 in vivo ubiquitination assay evidence A type of in vivo ubiquitination assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001224 knockin evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001090 knockin evidence A type of knockin evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001225 knockout evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001091 knockout phenotypic evidence A type of knockout evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001226 lipid binding assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001092 lipid binding assay evidence A type of lipid binding assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001227 luminescence-based mammalian interactome mapping assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001093 luminescence-based mammalian interactome mapping assay evidence A type of LUMIER assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001228 macroscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001094 macroscopy evidence A type of macroscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001229 mammalian 2-hybrid assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001095 mammalian 2-hybrid assay evidence A type of mammalian 2-hybrid assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001230 mass spectrometry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001096 mass spectrometry evidence A type of mass spectrometry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001231 medical imaging evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001256 imaging assay evidence used in manual assertion A type of medical imaging evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001232 microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001256 imaging assay evidence used in manual assertion A type of microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001233 motility wound healing assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001099 motility wound healing assay evidence A type of motility wound healing assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001234 MTS assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001100 MTS assay evidence A type of MTS assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001235 MTT assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001101 MTT assay evidence A type of MTT assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001236 multiplex bead-based immunoassay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001102 multiplex bead-based immunoassay evidence A type of multiplex bead-based immunoassay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001237 natural variation mutant evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001103 natural variation mutant evidence A type of natural variation mutant evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001238 nuclear magnetic resonance evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001105 nuclear magnetic resonance evidence A type of nuclear magnetic resonance evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001239 nuclear fragmentation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001104 nuclear fragmentation evidence A type of nuclear fragmentation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001240 nuclease protection assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001106 nuclease protection assay evidence A type of nuclease protection assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001241 nucleotide analog incorporation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001107 nucleotide analog incorporation assay evidence A type of nucleotide analog incorporation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001242 phage display evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001108 phage display evidence A type of phage display evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001243 phosphoamino acid analysis evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001109 phosphoamino acid analysis evidence A type of phosphoamino acid analysis evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001244 peptide affinity enrichment evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001110 peptide affinity enrichment evidence A type of peptide affinity enrichment evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001245 peptide array evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001112 peptide array evidence A type of peptide array evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001246 physical examination evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001111 physical examination evidence A type of physical examination evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001247 point mutation phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001113 point mutation phenotypic evidence A type of point mutation phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001248 propidium iodide staining evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001114 propidium iodide staining evidence A type of propidium iodide staining evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001249 fluorescence evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001115 fluorescence evidence A type of protein detection by fluorescence evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001250 protein dot blot assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001116 protein dot blot assay evidence A type of protein dot blot assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001251 protein microarray evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001117 protein microarray evidence A type of protein microarray evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001252 protein sequencing assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001118 protein sequencing assay evidence A type of protein sequencing assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001253 quantitative mass spectrometry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001230 mass spectrometry evidence used in manual assertion A type of quantitative mass spectrometry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001254 radioisotope assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001120 radioisotope assay evidence A type of radioisotope assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001255 radioimmunoassay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001121 radioimmunoassay evidence A type of radioimmunoassay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001256 imaging assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000324 imaging assay evidence A type of imaging assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001257 restriction fragment detection evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001124 restriction fragment detection evidence A type of restriction fragment detection evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001258 spectrophotometry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001126 spectrophotometry evidence A type of spectrophotometry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001259 syngeneic transplantation experiment evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001128 syngeneic transplantation experiment evidence A type of syngeneic transplantation experiment evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001260 xenotransplantation phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001137 xenotransplantation phenotypic evidence A type of xenotransplantation phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001261 WST-1 assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001136 WST-1 assay evidence A type of WST-1 assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001263 urine test evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001134 urine test evidence A type of urine test evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001264 terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001133 terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence A type of terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001265 tryptic phosphopeptide mapping assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001230 mass spectrometry evidence used in manual assertion A type of tryptic phosphopeptide mapping assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001266 transgenic organism evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001131 transgenic organism evidence A type of transgenic organism evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001267 tissue microarray evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001172 cytochemistry evidence used in manual assertion A type of tissue microarray evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001268 TACE activity assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005801 enzymatic activity assay evidence used in manual assertion A type of TACE activity assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001269 surface plasmon resonance evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001127 surface plasmon resonance evidence A type of surface plasmon resonance evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001270 restriction landmark genomic scanning evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of restriction landmark genome scanning evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001271 resonant mirror biosensor evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001123 resonant mirror biosensor evidence A type of resonant mirror biosensor evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001272 high-performance liquid chromatography evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007680 chromatography evidence used in manual assertion A type of high-performance liquid chromatography evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001273 ectopic expression evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000017 ectopic expression evidence A type of ectopic expression evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001601 protein-oligonucleotide microarray binding evidence http://purl.obolibrary.org/obo/ECO_0000136 nucleic acid binding evidence A type of protein-binding evidence that detects binding of a tagged protein to an array of oligonucleotide probes representing potential binding sites. http://purl.obolibrary.org/obo/ECO_0001603 cell staining evidence http://purl.obolibrary.org/obo/ECO_0001565 cell-based assay evidence A type of cell-based assay evidence in which living cells are stained with a vital stain, i.e. dye that can be used on living cells without causing cell death. http://purl.obolibrary.org/obo/ECO_0001801 alkaline phosphatase reporter gene assay evidence http://purl.obolibrary.org/obo/ECO_0000049 reporter gene assay evidence A type of reporter gene assay evidence based on the fusion of selected genes with the phoA gene to express alkaline phosphatase in periplasmic space for protein tracing. http://purl.obolibrary.org/obo/ECO_0001802 beta-galactosidase reporter gene assay evidence http://purl.obolibrary.org/obo/ECO_0000049 reporter gene assay evidence A type of reporter gene assay evidence based on the fusion of the lacZ gene to a specific promoter for the expression of beta-galactosidase which will appear blue when grown on a X-gal medium. http://purl.obolibrary.org/obo/ECO_0001803 chloramphenicol acetyltransferase reporter gene assay evidence http://purl.obolibrary.org/obo/ECO_0000049 reporter gene assay evidence A type of reporter gene assay evidence based on the fusion of the CAT gene to a specific promoter for the expression of chloramphenicol acetyltransferase which confers resistance to the chloramphenicol antibiotic. http://purl.obolibrary.org/obo/ECO_0001804 beta-glucuronidase reporter gene assay evidence http://purl.obolibrary.org/obo/ECO_0000049 reporter gene assay evidence A type of reporter gene assay evidence where the beta-glucuronidase enzyme from Escherichia coli is used as the reporter to transform non-fluorescent substrates into fluorescents for detection. http://purl.obolibrary.org/obo/ECO_0001805 luciferase reporter gene assay evidence http://purl.obolibrary.org/obo/ECO_0000049 reporter gene assay evidence A type of reporter gene assay evidence where luciferase, an oxidative enzyme, is used as the reporter to detect a gene product with bioluminescence. http://purl.obolibrary.org/obo/ECO_0001806 chromatin immunoprecipitation- exonuclease evidence http://purl.obolibrary.org/obo/ECO_0000226 chromatin immunoprecipitation evidence A type of chromatin immunoprecipitation evidence that uses gama-exonuclease to digest TF-unbound DNA after ChIP for the identification of transcription factor binding site locations with high-resolution data. http://purl.obolibrary.org/obo/ECO_0001807 electrophoretic mobility shift assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000096 electrophoretic mobility shift assay evidence A type of electrophoretic mobility shift that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001808 reverse transcription polymerase chain reaction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000109 reverse transcription polymerase chain reaction evidence A type of reverse transcription polymerase chain reaction evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001809 DNA affinity chromatography evidence http://purl.obolibrary.org/obo/ECO_0000325 chromatography evidence A type of chromatography evidence where immobilized promoter DNA is labeled and bound to a matrix through which a protein mixture is poured and then eluted to purify specific DNA binding proteins. http://purl.obolibrary.org/obo/ECO_0001810 DNAse footprinting evidence http://purl.obolibrary.org/obo/ECO_0000136 nucleic acid binding evidence A type of nucleic acid binding evidence where proteins that have been bound to DNA protect a binding site from enzymatic cleavage with DNAse, thereby detecting protein-DNA interactions. http://purl.obolibrary.org/obo/ECO_0001811 fluorescence anisotropy evidence http://purl.obolibrary.org/obo/ECO_0001115 fluorescence evidence A type of fluorescence evidence based on rapid and quantitative analysis of diverse molecular interactions, enzyme activities, and nucleic acid hybridization which uses a fluorophore to measure the binding constants and kinetics of reactions that cause a change in the rotational time of the molecules. http://purl.obolibrary.org/obo/ECO_0001812 genomic systematic evolution of ligands by exponential amplification evidence http://purl.obolibrary.org/obo/ECO_0000293 systematic evolution of ligands by exponential amplification evidence A type of systematic evolution of ligands by exponential amplification evidence based on a restricted genomic DNA library to identify naturally occurring genomic aptamers and RNA-protein interaction networks with RNA-binding protein as bait and high-throughput sequencing. http://purl.obolibrary.org/obo/ECO_0001813 heteronuclear single quantum coherence spectroscopy evidence http://purl.obolibrary.org/obo/ECO_0006163 nuclear magnetic resonance spectroscopy evidence A type of nuclear magnetic resonance spectroscopy evidence based on two-dimensional NMR for elucidation of the chemical structure of an isolated or synthesized chemical compound where the characteristic transfer magnetization of a proton to a nitrogen or carbon isotope is monitored by NMR, generating a specific peak in the spectrum. http://purl.obolibrary.org/obo/ECO_0001814 methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence http://purl.obolibrary.org/obo/ECO_0000136 nucleic acid binding evidence A type of nucleic acid binding evidence where the synthetic molecule methidiumpropyl-EDTA (MPE) is used to cleave ligand-protected DNA, followed by analysis of the restriction fragments to generate a footprint (i.e. size and location) of small molecule binding sites on the DNA. http://purl.obolibrary.org/obo/ECO_0001815 copper-phenanthroline footprinting evidence http://purl.obolibrary.org/obo/ECO_0000136 nucleic acid binding evidence A type of nucleic acid binding evidence where nucleic acid that has been bound to protein is cleaved with 1,10-phenanthroline-copper complex resulting in a high-resolution footprint of sequence-specific protein-DNA contacts. http://purl.obolibrary.org/obo/ECO_0001816 green fluorescent protein reporter gene assay evidence http://purl.obolibrary.org/obo/ECO_0000049 reporter gene assay evidence A type of reporter gene assay evidence based on the fusion of select genes with the green fluorescent protein (GFP) gene for detection with bioluminescence of the gene product when exposed to blue ultraviolet light. http://purl.obolibrary.org/obo/ECO_0001817 glutathione S-transferase pull-down assay evidence http://purl.obolibrary.org/obo/ECO_0000025 bait-prey hybrid interaction evidence A type of bait-prey hybrid interaction evidence where a gene is fused with the GST gene and the resulting recombinant bait protein is captured on an immobilized Glutathione affinity ligand and incubated with prey protein to identify and characterize protein-protein interactions. http://purl.obolibrary.org/obo/ECO_0001818 hydroxyl-radical footprinting evidence http://purl.obolibrary.org/obo/ECO_0000136 nucleic acid binding evidence A type of nucleic acid binding evidence used to identify protein-binding sites on the DNA molecule where DNA that has been bound to protein is digested with hydroxyl radical produced by reduction of hydrogen peroxide with iron (II), followed by separating the cleavage products on a denaturing electrophoresis gel. http://purl.obolibrary.org/obo/ECO_0001819 primer extension assay evidence http://purl.obolibrary.org/obo/ECO_0000009 transcript expression evidence A type of transcript expression evidence used to determine expression levels of mRNA where a labeled synthetic oligonucleotide primer is annealed to mRNA downstream of the presumed transcription start site of a gene. The mRNA is extended with reverse transcriptase, producing cDNA which is electrophoresed, and the band size is enables determination of the 5' end of the mRNA (i.e. the transcription start site). http://purl.obolibrary.org/obo/ECO_0001820 rapid amplification of cDNA ends polymerase chain reaction evidence http://purl.obolibrary.org/obo/ECO_0000219 nucleotide sequencing assay evidence A type of nucleotide sequencing assay evidence in which RT-PCR (cDNA synthesis) is first used to produce a cDNA copy of a region of the RNA transcript being investigated followed by PCR to capture either the unknown 5' or 3' end of the transcript for sequencing, depending on whether 5' RACE-PCR or 3' RACE-PCR are being undertaken. http://purl.obolibrary.org/obo/ECO_0001822 survival rate analysis evidence http://purl.obolibrary.org/obo/ECO_0001091 knockout phenotypic evidence A type of knockout evidence based on the survival of an organism in a particular environment where a gene for an enzyme or regulator is knocked out and results are used as a natural reporter. http://purl.obolibrary.org/obo/ECO_0001823 x-ray crystallography evidence http://purl.obolibrary.org/obo/ECO_0001034 crystallography evidence A type of crystallography evidence where a purified sample at high concentration is crystallised and the crystals are exposed to an x ray beam to obtain three dimensional molecular structure of proteins and biological macromolecules. http://purl.obolibrary.org/obo/ECO_0001824 DNA adenine methyltransferase identification evidence http://purl.obolibrary.org/obo/ECO_0000023 affinity evidence A type of affinity evidence used to identify DNA binding sites in eukaryotes where Escherichia coli DNA adenine methyltransferase (Dam) is fused to a transcription factor, co-factor, chromatin-associated protein, or nuclear-associated protein, followed by a methyl-dependent PCR to localize methyltransferase in the region of the binding site. http://purl.obolibrary.org/obo/ECO_0001825 isothermal titration calorimetry evidence http://purl.obolibrary.org/obo/ECO_0000023 affinity evidence A type of affinity evidence where the absorbed or released heat of a biomolecular binding event is directly measured in a reference cell and sample cell after the addition of a ligand using a microcalorimeter for a complete thermodynamic profile of the molecular interaction. http://purl.obolibrary.org/obo/ECO_0001826 ultraviolet light footprinting evidence http://purl.obolibrary.org/obo/ECO_0000136 nucleic acid binding evidence A type of nucleic acid binding evidence where DNA is non-specifically fragmented with ultraviolet light while protein-bound regions are protected from UV damage and strand breakage patterns are analyzed by PAGE and sequenced to detect protein-DNA contacts. http://purl.obolibrary.org/obo/ECO_0001827 methylation interference footprinting evidence http://purl.obolibrary.org/obo/ECO_0000136 nucleic acid binding evidence A type of nucleic acid binding evidence used to identify protein binding sites on the DNA molecule where multiple copies of a DNA fragment containing a putative TF-binding site are randomly methylated with dimethyl sulfate (DMS) and cleaved at the methyl group, followed by separating the cleavage products on a denaturing electrophoresis gel. http://purl.obolibrary.org/obo/ECO_0001828 inference of sequence features from visual inspection used in manual assertion http://purl.obolibrary.org/obo/ECO_0007850 inference of sequence features from visual inspection A type of inference of sequence features from visual inspection that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001829 ferric uptake regulator titration assay evidence http://purl.obolibrary.org/obo/ECO_0000049 reporter gene assay evidence A type of reporter gene assay evidence used to locate and isolate Fur sites where a plasmid library is created and cloned into a cell line with a ferric uptake regulator (Fur)-repressed reporter and the Fur protein is titrated away from its binding site on the reporter, after which the cell is isolated and plasmid extracted for further sequence analysis. http://purl.obolibrary.org/obo/ECO_0005004 cell viability assay evidence http://purl.obolibrary.org/obo/ECO_0001565 cell-based assay evidence A type of cell-based assay evidence resulting from analyzing the ability of cells to survive or live successfully. http://purl.obolibrary.org/obo/ECO_0005007 cell proliferation assay evidence http://purl.obolibrary.org/obo/ECO_0001565 cell-based assay evidence A type of cell-based assay evidence resulting from the measurement of the number of cells, which is a reflection of the balance between cell division and cell loss through cell differentiation or cell death. http://purl.obolibrary.org/obo/ECO_0005008 DNA synthesis cell proliferation assay evidence http://purl.obolibrary.org/obo/ECO_0005007 cell proliferation assay evidence A type of cell proliferation assay evidence resulting from the measure of DNA synthesis in the presece of a label. http://purl.obolibrary.org/obo/ECO_0005011 ATP bioluminescence assay evidence http://purl.obolibrary.org/obo/ECO_0005004 cell viability assay evidence A type of cell viability assay evidence resulting from the analysis of ATP concentration (by measuring light intensity) when luciferase catalyzes the oxidation of luciferin in the presence of ATP, magnesium ions and molecular oxygen. http://purl.obolibrary.org/obo/ECO_0005012 cytotoxicity assay evidence http://purl.obolibrary.org/obo/ECO_0001565 cell-based assay evidence A type of cell-based assay evidence resulting from the ability of an agent to induce cellular necrosis or apoptosis and the subsequent analysis of cell death mechanism. http://purl.obolibrary.org/obo/ECO_0005019 staining evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence resulting from the use of stains or dyes to aid in analysis of a microscopic image. http://purl.obolibrary.org/obo/ECO_0005021 chemotaxis assay evidence http://purl.obolibrary.org/obo/ECO_0001565 cell-based assay evidence A type of cell-based assay evidence resulting from the evaluation of chemotactic ability (i.e. the movement, or lack of movement, in response to a chemical stimulus) of prokaryotic or eukaryotic cells. http://purl.obolibrary.org/obo/ECO_0005027 genetic transformation evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence resulting from the conversion of one genotype into another by the introduction of exogenous DNA. http://purl.obolibrary.org/obo/ECO_0005031 structure determination evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence resulting from the visualization and examination of chemical and/or molecular structures. http://purl.obolibrary.org/obo/ECO_0005033 electron microscopy evidence http://purl.obolibrary.org/obo/ECO_0005031 structure determination evidence A type of microscopy evidence and structure determination evidence resulting from an electron beam utilized to create an image. http://purl.obolibrary.org/obo/ECO_0005034 apoptotic assay evidence http://purl.obolibrary.org/obo/ECO_0001565 cell-based assay evidence A type of cell-based assay evidence resulting from the occurrence, visualization, and/or analysis of apoptosis. http://purl.obolibrary.org/obo/ECO_0005500 in vivo polyADP-ribosylation assay evidence http://purl.obolibrary.org/obo/ECO_0001557 polyADP-ribosylation assay evidence A type of polyADP-ribosylation assay evidence used in an in vivo experiment. http://purl.obolibrary.org/obo/ECO_0005501 in vivo polyADP-ribosylation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005500 in vivo polyADP-ribosylation assay evidence A type of in vivo polyADP-ribosylation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005502 ex vivo assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence derived by studying an organ, tissues, or cells taken from an organism and studied in an external environment with the minimum alteration of natural conditions. http://purl.obolibrary.org/obo/ECO_0005503 two-dimensional polyacrylamide gel electrophoresis evidence http://purl.obolibrary.org/obo/ECO_0000337 gel electrophoresis evidence A type of gel electrophoresis evidence that involves one dimension electrophoresis where biomolecules are electrophoresed on a low percentage polyacrylamide gel to be separated in proportion to their mass or isoelectric point, followed by high voltage electrophoresis on a higher percentage gel in the presence of ethidium bromide to alter non-linear molecular shape. http://purl.obolibrary.org/obo/ECO_0005504 spectrometry evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence resulting from the use of a spectrometer. http://purl.obolibrary.org/obo/ECO_0005505 regular expression motif search evidence http://purl.obolibrary.org/obo/ECO_0000028 motif similarity evidence A type of motif similarity evidence where the motif, a local alignment of sequences, is represented by a regular expression, which captures the variability of nucleotides at each position in a discrete form and can then be used to search genetic sequences by identifying matches to the regular expression. http://purl.obolibrary.org/obo/ECO_0005506 missense mutation phenotypic evidence http://purl.obolibrary.org/obo/ECO_0001113 point mutation phenotypic evidence A type of point mutation phenotypic evidence resulting from a change in a base (or bases) causing the substitution of one amino acid for another. http://purl.obolibrary.org/obo/ECO_0005507 nonsense mutation phenotypic evidence http://purl.obolibrary.org/obo/ECO_0001113 point mutation phenotypic evidence A type of point mutation phenotypic evidence resulting in an incomplete and usually nonfunctional protein product because of a premature stop codon. http://purl.obolibrary.org/obo/ECO_0005508 silent mutation evidence http://purl.obolibrary.org/obo/ECO_0001113 point mutation phenotypic evidence A type of point mutation phenotypic evidence resulting from the change of a DNA nucleotide that does not cause any changes in the amino acid sequence. http://purl.obolibrary.org/obo/ECO_0005509 insertion mutation phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence resulting from a mutation in which there is an insertion of one or more contiguous nucleotides. http://purl.obolibrary.org/obo/ECO_0005511 duplication mutation evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence resulting from a mutation in which inserted nucleotide(s) have a sequence identical to, or derived from, adjacent nucleotides. http://purl.obolibrary.org/obo/ECO_0005512 frameshift mutation phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence resulting from any mutation that causes an insertion or deletion of nucleotides in a DNA sequence that is not divisible by three and therefore changes the codon reading frame and the translated amino acids. http://purl.obolibrary.org/obo/ECO_0005513 repeat expansion mutation phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence resulting from a mutation that increases the number of times that a DNA sequence is repeated. http://purl.obolibrary.org/obo/ECO_0005514 splice site mutation phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence resulting from a mutation in which there is a change in nucleotides at a splice site. http://purl.obolibrary.org/obo/ECO_0005515 translocation mutation phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence resulting from a mutation in which a region of a chromosome is transferred to a nonhomologous chromosome. http://purl.obolibrary.org/obo/ECO_0005516 molecule detection assay evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence resulting from the detection and analysis of molecular markers. http://purl.obolibrary.org/obo/ECO_0005517 protein detection assay evidence http://purl.obolibrary.org/obo/ECO_0005516 molecule detection assay evidence A type of molecule detection assay evidence resulting from the detection and quantification of a protein, or multiple proteins. http://purl.obolibrary.org/obo/ECO_0005518 RNA detection assay evidence http://purl.obolibrary.org/obo/ECO_0005516 molecule detection assay evidence A type of molecule detection assay evidence resulting from the detection of specific RNAs. http://purl.obolibrary.org/obo/ECO_0005519 DNA detection assay evidence http://purl.obolibrary.org/obo/ECO_0005516 molecule detection assay evidence A type of molecule detection assay evidence resulting from the detection and visualization of a sequence of DNA. http://purl.obolibrary.org/obo/ECO_0005520 interferometric reflectance imaging sensor evidence http://purl.obolibrary.org/obo/ECO_0005516 molecule detection assay evidence A type of molecule detection assay evidence based on changes in surface-reflected light due to ligand binding on the surface of a semi-transparent substrate to detect proteins, DNA, and other biological material in a multiplexed, high-throughput microarray format without labels. http://purl.obolibrary.org/obo/ECO_0005521 S1 nuclease protection assay evidence http://purl.obolibrary.org/obo/ECO_0001106 nuclease protection assay evidence A type of nuclease protection assay evidence where RNA is hybridized with complementary DNA probes and exposed to S1 nuclease for unbound RNA degradation after which the intact RNA is run on a gel for probe size determination and RNA identification to detect and map specific RNAs in a complex mixture of total cellular RNA. http://purl.obolibrary.org/obo/ECO_0005522 DNA dot blot assay evidence http://purl.obolibrary.org/obo/ECO_0005519 DNA detection assay evidence A type of DNA detection assay evidence resulting from evaluation of the relative abundance of target sequences hybridizing to DNA samples that are immobilized on a nitrocellulose or nylon membrane. http://purl.obolibrary.org/obo/ECO_0005523 DNA dot blot assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005522 DNA dot blot assay evidence A type of DNA dot blot assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005526 matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence http://purl.obolibrary.org/obo/ECO_0005517 protein detection assay evidence A type of protein detection assay evidence where soft ionization is used for the analysis of biomolecules for mass determination in a three step process of plate preparation, radiation, and ionization with high throughput technology. http://purl.obolibrary.org/obo/ECO_0005527 matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005526 matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005528 site-directed mutagenesis phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype phenotype evidence based on target-specified mutagenesis for the characterization of various gene and protein interactions, structures, and functions where oligonucleotide primers are used to alter a nucleotide sequence. http://purl.obolibrary.org/obo/ECO_0005529 random mutagenesis phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence where a mutagenic product or process is used to alter the nucleotide sequence and thus induce genetic mutation. Examples include, but are not limited to mutagenic compounds, irradiation, PCR w/ degenerate primers, etc. http://purl.obolibrary.org/obo/ECO_0005530 random mutagenesis evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005529 random mutagenesis phenotypic evidence A type of random mutagenesis phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005531 motif discovery evidence http://purl.obolibrary.org/obo/ECO_0005555 multiple sequence alignment evidence A type of multiple sequence alignment evidence based on a set of algorithms that infer over-represented motifs from a set of biological sequences returning one or more local multiple sequence alignment defining the inferred motifs. http://purl.obolibrary.org/obo/ECO_0005532 consensus search evidence http://purl.obolibrary.org/obo/ECO_0000028 motif similarity evidence A type of motif similarity evidence where the motif is represented by a consensus sequence which incorporates the most frequent nucleotide at each position acting as a generic representative for the sequences in the alignment, and is compared to the genetic sequence for a perfect or imperfect match. http://purl.obolibrary.org/obo/ECO_0005533 phylogenetic footprinting evidence http://purl.obolibrary.org/obo/ECO_0005531 motif discovery evidence A type of motif discovery evidence based on a set of algorithms that infer motifs in non-coding DNA sequences from the conservation of functional regulatory sites through evolution at a higher rate than their local surroundings for the identification of an evolutionary 'footprint'. http://purl.obolibrary.org/obo/ECO_0005534 comparative genomics motif search evidence http://purl.obolibrary.org/obo/ECO_0000028 motif similarity evidence A type of motif similarity evidence based on comparative genomics which uses a search method to scan a set of genomes for instances of a specific motif, and then applies a comparative criterion to analyze the collective strength of these predictions, based on the assumption that functional instances of the motif will be preserved by natural selection and their prediction will therefore be consistent across genomes. http://purl.obolibrary.org/obo/ECO_0005535 machine learning prediction of motif instance evidence http://purl.obolibrary.org/obo/ECO_0000202 match to sequence model evidence A type of match to sequence model evidence which captures the application of a broad suite of supervised and unsupervised machine learning algorithms trained on known motif instances and applied to the prediction of novel putative instances of a motif in a set of biological sequences. http://purl.obolibrary.org/obo/ECO_0005536 position-specific scoring matrix motif search evidence http://purl.obolibrary.org/obo/ECO_0000028 motif similarity evidence A type of motif similarity evidence where the motif is represented by a position-specific frequency matrix (PSFM) that specifies the frequency of each base at each position of the motif, from which a position-specific scoring matrix (PSSM) can be derived under the framework of a log-likelihood ratio providing a scoring system based on the likelihood of a sequence given the motif, versus a genomic-frequency based null hypothesis. http://purl.obolibrary.org/obo/ECO_0005537 xylE reporter gene assay evidence http://purl.obolibrary.org/obo/ECO_0000049 reporter gene assay evidence A type of reporter gene assay evidence based on the fusion of the xylE gene to a specific promoter for the expression of catechol 2,3-dioxygenase that converts the colorless catechol substrate to yellow 2-hydroxymuconic semialdehyde for identification of the expression of a particular gene. http://purl.obolibrary.org/obo/ECO_0005542 biological system reconstruction evidence by experimental evidence from single species used in manual assertion http://purl.obolibrary.org/obo/ECO_0005553 biological system reconstruction evidence by experimental evidence from single species A type of biological system reconstruction evidence by experimental evidence from single species that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005543 biological system reconstruction evidence by experimental evidence from mixed species used in manual assertion http://purl.obolibrary.org/obo/ECO_0005552 biological system reconstruction evidence by experimental evidence from mixed species A type of biological system reconstruction evidence by experimental evidence from mixed species that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005544 biological system reconstruction evidence based on orthology evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005610 biological system reconstruction evidence based on homology evidence used in manual assertion A type of biological system reconstruction evidence based on orthology evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005545 biological system reconstruction evidence based on orthology evidence http://purl.obolibrary.org/obo/ECO_0005549 biological system reconstruction evidence based on homology evidence A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by orthology from an existing experimentally supported model to a process, pathway or complex in another species. http://purl.obolibrary.org/obo/ECO_0005546 biological system reconstruction evidence based on paralogy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005610 biological system reconstruction evidence based on homology evidence used in manual assertion A type of biological system reconstruction evidence based on paralogy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005547 biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion http://purl.obolibrary.org/obo/ECO_0005548 biological system reconstruction evidence based on inference from background scientific knowledge A type of biological system reconstruction evidence based on inference from background scientific knowledge that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005548 biological system reconstruction evidence based on inference from background scientific knowledge http://purl.obolibrary.org/obo/ECO_0000088 biological system reconstruction evidence A type of biological system reconstruction based solely on background scientific knowledge of that biological system. http://purl.obolibrary.org/obo/ECO_0005549 biological system reconstruction evidence based on homology evidence http://purl.obolibrary.org/obo/ECO_0000088 biological system reconstruction evidence A type of biological system reconstruction where the evidence is inferred by homology based on conservation of sequence, function, and composition from an existing experimentally supported model to a process, pathway, or complex. http://purl.obolibrary.org/obo/ECO_0005550 biological system reconstruction evidence based on paralogy evidence http://purl.obolibrary.org/obo/ECO_0005549 biological system reconstruction evidence based on homology evidence A type of biological system reconstruction evidence based on homology evidence where the evidence is inferred by paralogy from an existing experimentally supported model to a process, pathway, or complex in the same species. http://purl.obolibrary.org/obo/ECO_0005551 biological system reconstruction evidence by experimental evidence http://purl.obolibrary.org/obo/ECO_0000088 biological system reconstruction evidence A type of biological system reconstruction evidence that uses experimental evidence as support. http://purl.obolibrary.org/obo/ECO_0005552 biological system reconstruction evidence by experimental evidence from mixed species http://purl.obolibrary.org/obo/ECO_0005551 biological system reconstruction evidence by experimental evidence A type of biological system reconstruction evidence where the experimental evidence is derived by using a mix of species used in the same experiment. http://purl.obolibrary.org/obo/ECO_0005553 biological system reconstruction evidence by experimental evidence from single species http://purl.obolibrary.org/obo/ECO_0005551 biological system reconstruction evidence by experimental evidence A type of biological system reconstruction where the experimental evidence is derived by using multiple experiments in a single species. http://purl.obolibrary.org/obo/ECO_0005554 pairwise sequence alignment evidence http://purl.obolibrary.org/obo/ECO_0000200 sequence alignment evidence A type of sequence alignment evidence where two sequences are aligned to identify regions of similarity that may indicate functional, structural and/or evolutionary relationships between two biological sequences (protein or nucleic acid). http://purl.obolibrary.org/obo/ECO_0005555 multiple sequence alignment evidence http://purl.obolibrary.org/obo/ECO_0000200 sequence alignment evidence A type of sequence alignment evidence where three or more biological sequences (protein or nucleic acid) of similar length are aligned to infer homology and the evolutionary relationships between the sequences studied. http://purl.obolibrary.org/obo/ECO_0005556 multiple sequence alignment evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005555 multiple sequence alignment evidence A type of multiple sequence alignment evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005557 multiple sequence alignment evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005555 multiple sequence alignment evidence A type of multiple sequence alignment evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005558 motif discovery evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005556 multiple sequence alignment evidence used in manual assertion A type of motif discovery evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005559 motif discovery evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005557 multiple sequence alignment evidence used in automatic assertion A type of motif discovery evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005560 pairwise sequence alignment evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005554 pairwise sequence alignment evidence A type of pairwise sequence alignment evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005561 pairwise sequence alignment evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005554 pairwise sequence alignment evidence A type of pairwise sequence alignment evidence that is used in an automatic evidence. http://purl.obolibrary.org/obo/ECO_0005562 in-gel protein kinase assay evidence http://purl.obolibrary.org/obo/ECO_0001558 protein kinase assay evidence A type of protein kinase assay evidence resulting from the evaluation of the phosphorylation activity of kinase on a gel-incorporated kinase substrate. http://purl.obolibrary.org/obo/ECO_0005563 macroscopic current trace evidence http://purl.obolibrary.org/obo/ECO_0005576 voltage clamp recording evidence A type of voltage clamp recording evidence that involves recording trace of the current (pA) going through the analyzed ion channels determined from voltage clamp current recording. http://purl.obolibrary.org/obo/ECO_0005564 current density evidence http://purl.obolibrary.org/obo/ECO_0000164 electrophysiology assay evidence A type of electrophysiology assay evidence that involves measuring the amount of current (pA) going through the ion channels per unit of membrane. http://purl.obolibrary.org/obo/ECO_0005566 sustained current evidence http://purl.obolibrary.org/obo/ECO_0000164 electrophysiology assay evidence A type of electrophysiology assay evidence that involves the measurement of the current that persists after the fast and slow inactivation of the analyzed channels. http://purl.obolibrary.org/obo/ECO_0005570 use dependence of inactivation evidence http://purl.obolibrary.org/obo/ECO_0000164 electrophysiology assay evidence A type of electrophysiology assay evidence in which the amount of current remaining after a series of depolarization at different frequencies described as the normalized current related to the time course. http://purl.obolibrary.org/obo/ECO_0005571 current clamp recording evidence http://purl.obolibrary.org/obo/ECO_0000164 electrophysiology assay evidence A type of electrophysiology assay evidence that involves recording the change in membrane potential caused by applying specific current to the cell. http://purl.obolibrary.org/obo/ECO_0005572 whole-cell voltage clamp recording evidence http://purl.obolibrary.org/obo/ECO_0005576 voltage clamp recording evidence A type of voltage clamp recording evidence that involves direct measurement of ionic current across a membrane while controlling the membrane potential by maintaining the voltage. http://purl.obolibrary.org/obo/ECO_0005573 cell-attached single-channel recording evidence http://purl.obolibrary.org/obo/ECO_0006012 patch-clamp recording evidence A type of patch-clamp recording evidence that involves isolation of a patch of membrane electrically from the external solution and to record current flowing into the patch and this is achieved by pressing a fire-polished glass pipette, which has been filled with a suitable electrolyte solution, against the surface of a cell and applying light suction. http://purl.obolibrary.org/obo/ECO_0005574 cell-detached inside-out single-channel recording evidence http://purl.obolibrary.org/obo/ECO_0006012 patch-clamp recording evidence A type of patch-clamp recording evidence that involves study of individual ion channels by gently pulling the patch of membrane away from the cell, and the patch remains attached to the pipette with its cytoplasmic surface exposed to the bathing solution. http://purl.obolibrary.org/obo/ECO_0005575 reconstituted bilayer single-channel patch recording evidence http://purl.obolibrary.org/obo/ECO_0006012 patch-clamp recording evidence A type of patch-clamp reocrding evidence that involves ion channel analysis by incorporating the ion channels into liposomes suitable for patch-clamping which allows isolation and study of a specific population of channels into that bilayer membrane. http://purl.obolibrary.org/obo/ECO_0005576 voltage clamp recording evidence http://purl.obolibrary.org/obo/ECO_0000164 electrophysiology assay evidence A type of electrophysiology assay evidence that involves recording membrane potential by controlling the membrane voltage and measuring the transmembrane current required to maintain that voltage. http://purl.obolibrary.org/obo/ECO_0005577 electroencephalography recording evidence http://purl.obolibrary.org/obo/ECO_0000164 electrophysiology assay evidence A type of electrophysiology assay evidence that involves recording the electrical activity of the brain by means of electrodes placed on the surface of the head. http://purl.obolibrary.org/obo/ECO_0005578 super-resolution microscopy evidence http://purl.obolibrary.org/obo/ECO_0001098 microscopy evidence A type of microscopy evidence where the images are taken with a higher resolution than the diffraction limit that help in studying nanoscopic sub-cellular structures. http://purl.obolibrary.org/obo/ECO_0005579 immunogold labelling evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006031 immunolocalization evidence used in manual assertion A type of immunogold labelling evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005580 flow cytometry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of flow cytometry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005581 enzyme-linked immunoabsorbent assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of enzyme-linked immunoabsorbent assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005582 cell-detached outside-out single-channel recording evidence http://purl.obolibrary.org/obo/ECO_0006012 patch-clamp recording evidence A type of patch-clamp recording evidence where a part of cellular membrane is detached from the cell and whose external face is exposed to the extracellular medium which allows isolating and studying a single or at least a small number of channels into that fragment (patch). http://purl.obolibrary.org/obo/ECO_0005583 cut-open oocyte voltage clamp recording evidence http://purl.obolibrary.org/obo/ECO_0005576 voltage clamp recording evidence A type of voltage clamp recording evidence that involves inserting the oocyte in a chamber that separates the surface into 3 regions where the top portion of the oocyte membrane is the region that is clamped from which currents are actually recorded while the bottom portion is the region of the oocyte that is cut-open, making it possible to inject current intracellularly through a low resistance pathway. http://purl.obolibrary.org/obo/ECO_0005584 macropatch voltage clamp recording evidence http://purl.obolibrary.org/obo/ECO_0005576 voltage clamp recording evidence A type of voltage clamp recording evidence where recording is done on a fragment of the cell membrane (attached to or detached from the cell) using a large tip diameter pipette that allows isolating and studying a small number of channels into that fragment (patch). http://purl.obolibrary.org/obo/ECO_0005585 high throughput mass spectrometry evidence http://purl.obolibrary.org/obo/ECO_0001096 mass spectrometry evidence A type of mass spectrometry evidence where mass-to-charge ratio (m/z) of gas-phase ions is measured which is used to identify and quantify molecules in complex solutions within the framework of high throughput studies. http://purl.obolibrary.org/obo/ECO_0005586 high throughput mass spectrometry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005585 high throughput mass spectrometry evidence A type of high throughput mass spectrometry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005587 confocal microscopy evidence http://purl.obolibrary.org/obo/ECO_0001098 microscopy evidence A type of microscopy evidence where sharp images are taken by excluding most of the light from the specimen that is not from the microscope's focal plane, thus allowing better contrast and less haze, which makes it possible to build three-dimensional reconstructions of a volume of the specimen by assembling a series of thin slices taken along the vertical axis. http://purl.obolibrary.org/obo/ECO_0005588 wide-field microscopy evidence http://purl.obolibrary.org/obo/ECO_0001098 microscopy evidence A type of microscopy evidence where imaging of a thin cell or a tissue specimen is performed by full aperture emission of light gathered by the microscope's objective, which maximizes the recorded signal and simultaneously minimizes the required exposure times. http://purl.obolibrary.org/obo/ECO_0005589 confocal microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005587 confocal microscopy evidence A type confocal microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005590 wide-field microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005588 wide-field microscopy evidence A type of wide-field microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005591 immunogold labelling electron microscopy assay evidence http://purl.obolibrary.org/obo/ECO_0005033 electron microscopy evidence A type of immunogold labelling evidence where electron microscopy technique is used to detect the location of the antibodies labeled with colloidal gold particles. http://purl.obolibrary.org/obo/ECO_0005592 immunogold labelling electron microscopy assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006003 electron microscopy evidence used in manual assertion A type of immunogold labelling electron microscopy assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005593 immunodetection assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence that involves the use of antibodies to detect biomolecules. http://purl.obolibrary.org/obo/ECO_0005594 immunoperoxidase immunolocalization evidence http://purl.obolibrary.org/obo/ECO_0000087 immunolocalization evidence A type of immunolocalization evidence involving enzymatic detection of a peroxidase tagged antibody. http://purl.obolibrary.org/obo/ECO_0005595 immunoperoxidase immunolocalization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006031 immunolocalization evidence used in manual assertion A type of immunoperoxidase immunolocalization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005596 immunoperoxidase immunolocalization electron microscopy evidence http://purl.obolibrary.org/obo/ECO_0005594 immunoperoxidase immunolocalization evidence A type of immunoperoxidase immunolocalization evidence where electron microscopy technique is used for enzymatic detection of a peroxidase tagged antibody. http://purl.obolibrary.org/obo/ECO_0005597 immunoperoxidase immunolocalization electron microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006003 electron microscopy evidence used in manual assertion A type of immunoperoxidase immunolocalization electron microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005598 wide-field fluorescence microscopy evidence http://purl.obolibrary.org/obo/ECO_0005588 wide-field microscopy evidence A type of wide-field microscopy evidence where pure white and ultraviolet light are produced by a mercury lamp, passed through an optical filter (excitation filter), and directed to a sample via a dichroic mirror, followed by detection of the fluorescent light by a camera after it passes through an emission filter. http://purl.obolibrary.org/obo/ECO_0005599 immunofluorescence wide-field microscopy evidence http://purl.obolibrary.org/obo/ECO_0005598 wide-field fluorescence microscopy evidence A type of wide-field fluorescence microscopy evidence where fluorescently tagged antibodies are imaged that are used to bind to their antigens. http://purl.obolibrary.org/obo/ECO_0005600 immunofluorescence confocal microscopy evidence http://purl.obolibrary.org/obo/ECO_0005587 confocal microscopy evidence A type of confocal microscopy evidence where light passes through immunofluorescent-stained tissue sections or cells and then the emitted light passes through the dichroic mirror and is focused onto the pinhole for subsequent detection. http://purl.obolibrary.org/obo/ECO_0005601 immunofluorescence confocal microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005804 immunofluorescence evidence used in manual assertion A type of immunofluorescence confocal microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005603 protein mass spectrometry evidence http://purl.obolibrary.org/obo/ECO_0001096 mass spectrometry evidence A type of mass spectrometry evidence where one or more protein analyses are performed. http://purl.obolibrary.org/obo/ECO_0005604 cross-streak test evidence http://purl.obolibrary.org/obo/ECO_0005007 cell proliferation assay evidence A type of cell proliferation assay evidence where the investigated organism or a potential antagonist is inoculated so as to grow along the diameter line of the agar medium in a Petri dish, then, potentially susceptible microbes (usually bacteria) are streaked perpendicularly to the growth line and inhibition of growth of a test microbe in the vicinity of that line suggests that the antagonist secretes one or more diffusible antibiotics. http://purl.obolibrary.org/obo/ECO_0005605 disk diffusion test evidence http://purl.obolibrary.org/obo/ECO_0007083 zone of inhibition evidence A type of zone of inhibition evidence where sensitivity/resistance to an antimicrobial agent is tested by impregnating small filter disks with a known concentration of antimicrobial agent which is then placed on a Mueller-Hinton agar plate inoculated with the test organism. http://purl.obolibrary.org/obo/ECO_0005606 cell transfection experiment evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence where a foreign genetic material is introduced into the cell for study of gene function and regulation and protein function. http://purl.obolibrary.org/obo/ECO_0005607 tethered cell assay evidence http://purl.obolibrary.org/obo/ECO_0005021 chemotaxis assay evidence A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis is determined by observing the rotational behavior of the cells tethered to a coverslip by their flagella. http://purl.obolibrary.org/obo/ECO_0005608 tumble frequency assay evidence http://purl.obolibrary.org/obo/ECO_0005021 chemotaxis assay evidence A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis in swimming organisms is determined by measuring chemotaxis in response to sudden concentration changes from one uniform spatial environment to another. http://purl.obolibrary.org/obo/ECO_0005609 capillary assay evidence http://purl.obolibrary.org/obo/ECO_0005021 chemotaxis assay evidence A type of chemotaxis assay evidence where the presence or absence of positive or negative chemotaxis is determined by measuring the rate of accumulation of the cells into a capillary. http://purl.obolibrary.org/obo/ECO_0005610 biological system reconstruction evidence based on homology evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005549 biological system reconstruction evidence based on homology evidence A type of biological system reconstruction evidence based on homology evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005613 inference by association of genotype from phenotype http://purl.obolibrary.org/obo/ECO_0001237 natural variation mutant evidence used in manual assertion A type of natural variation mutant evidence used in manual assertion where alleles are directly linked to phenotype and Mendelian diseases as a result of various experiments. http://purl.obolibrary.org/obo/ECO_0005614 two-dimensional polyacrylamide gel electrophoresis evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005503 two-dimensional polyacrylamide gel electrophoresis evidence A type of two-dimensional polyacrylamide gel electrophoresis evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005615 alkaline phosphatase reporter gene assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001801 alkaline phosphatase reporter gene assay evidence A type of alkaline phosphatase reporter gene assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005616 beta-galactosidase reporter gene assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001802 beta-galactosidase reporter gene assay evidence A type of beta-galactosidase reporter gene assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005617 chloramphenicol acetyltransferase reporter gene assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001803 chloramphenicol acetyltransferase reporter gene assay evidence A type of chloramphenicol acetyltransferase reporter gene assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005618 chromatin immunoprecipitation-chip evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000230 chromatin immunoprecipitation-chip evidence A type of chromatin immunoprecipitation-chip evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005619 chromatin immunoprecipitation- exonuclease evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001806 chromatin immunoprecipitation- exonuclease evidence A type of chromatin immunoprecipitation- exonuclease evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005620 chromatin immunoprecipitation-PCR evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005644 immunoprecipitation evidence used in manual assertion A type of chromatin immunoprecipitation-PCR evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005621 chromatin immunoprecipitation-seq evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000229 chromatin immunoprecipitation-seq evidence A type of chromatin immunoprecipitation-seq evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005622 comparative genomics motif search evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005534 comparative genomics motif search evidence A type of comparative genomics motif search evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005623 comparative genomics motif search evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005534 comparative genomics motif search evidence A type of comparative genomics motif search evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005624 consensus search evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005532 consensus search evidence A type of consensus search evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005625 consensus search evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005532 consensus search evidence A type of consensus search evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005626 copper-phenanthroline footprinting evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001815 copper-phenanthroline footprinting evidence A type of copper-phenanthroline footprinting evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005627 DNA adenine methyltransferase identification evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001824 DNA adenine methyltransferase identification evidence A type of DNA adenine methyltransferase identification evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005628 DNA adenine methyltransferase identification evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007384 affinity evidence used in automatic assertion A type of DNA adenine methyltransferase identification evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005629 DNA affinity chromatography evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007680 chromatography evidence used in manual assertion A type of DNA affinity chromatography evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005630 cDNA to DNA expression microarray evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007301 expression microarray evidence used in automatic assertion A type of cDNA to DNA expression microarray evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005631 DNAse footprinting evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001810 DNAse footprinting evidence A type of DNAse footprinting evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005633 ferric uptake regulator titration assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001829 ferric uptake regulator titration assay evidence A type of ferric uptake regulator titration assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005634 genomic systematic evolution of ligands by exponential amplification evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001812 genomic systematic evolution of ligands by exponential amplification evidence A type of genomic systematic evolution of ligands by exponential amplification evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005635 genomic systematic evolution of ligands by exponential amplification evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007510 systematic evolution of ligands by exponential amplification evidence used in automatic assertion A type of genomic systematic evolution of ligands by exponential amplification evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005636 green fluorescent protein reporter gene assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001816 green fluorescent protein reporter gene assay evidence A type of green fluorescent protein reporter gene assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005637 green fluorescent protein reporter gene assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001816 green fluorescent protein reporter gene assay evidence A type of green fluorescent protein reporter gene assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005638 cell growth regulation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000095 cell growth regulation assay evidence A type of cell growth regulation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005639 cell growth regulation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000095 cell growth regulation assay evidence A type of cell growth regulation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005640 glutathione S-transferase pull-down assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001817 glutathione S-transferase pull-down assay evidence A type of glutathione S-transferase pull-down assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005641 beta-glucuronidase reporter gene assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001804 beta-glucuronidase reporter gene assay evidence A type of beta-glucuronidase reporter gene assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005642 heteronuclear single quantum coherence spectroscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006165 nuclear magnetic resonance spectroscopy evidence used in manual assertion A type of heteronuclear single quantum coherence spectroscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005643 hydroxyl-radical footprinting evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001818 hydroxyl-radical footprinting evidence A type of hydroxyl-radical footprinting evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005644 immunoprecipitation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000085 immunoprecipitation evidence A type of immunoprecipitation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005645 interferometric reflectance imaging sensor evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005520 interferometric reflectance imaging sensor evidence A type of interferometric reflectance imaging sensor evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005646 interferometric reflectance imaging sensor evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005520 interferometric reflectance imaging sensor evidence A type of interferometric reflectance imaging sensor evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005647 isothermal titration calorimetry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001825 isothermal titration calorimetry evidence A type of isothermal titration calorimetry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005648 luciferase reporter gene assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001805 luciferase reporter gene assay evidence A type of luciferase reporter gene assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005649 machine learning prediction of motif instance evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005535 machine learning prediction of motif instance evidence A type of machine learning prediction of motif instance evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005650 machine learning prediction of motif instance evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005535 machine learning prediction of motif instance evidence A type of machine learning prediction of motif instance evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005651 matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005526 matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence A type of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005652 methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001814 methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence A type of methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005653 northern blot evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000291 transcript expression evidence used in manual assertion A type of northern blot evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005654 phylogenetic footprinting evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005558 motif discovery evidence used in manual assertion A type of phylogenetic footprinting evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005655 phylogenetic footprinting evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005559 motif discovery evidence used in automatic assertion A type of phylogenetic footprinting evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005656 methylation interference footprinting evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001827 methylation interference footprinting evidence A type of methylation interference footprinting evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005657 primer extension assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001819 primer extension assay evidence A type of primer extension assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005658 position-specific scoring matrix motif search evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005536 position-specific scoring matrix motif search evidence A type of position-specific scoring matrix motif search evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005659 position-specific scoring matrix motif search evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005536 position-specific scoring matrix motif search evidence A type of position-specific scoring matrix motif search evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005660 quantitative polymerase chain reaction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of quantitative polymerase chain reaction evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005661 rapid amplification of cDNA ends polymerase chain reaction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001820 rapid amplification of cDNA ends polymerase chain reaction evidence A type of rapid amplification of cDNA ends polymerase chain reaction evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005662 regular expression motif search evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005505 regular expression motif search evidence A type of regular expression motif search evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005663 regular expression motif search evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005505 regular expression motif search evidence A type of regular expression motif search evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005666 S1 nuclease protection assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005521 S1 nuclease protection assay evidence A type of S1 nuclease protection assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005667 site-directed mutagenesis phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005528 site-directed mutagenesis phenotypic evidence A type of site-directed phenotypic mutagenesis evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005668 survival rate analysis evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001822 survival rate analysis evidence A type of survival rate analysis evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005669 ultraviolet light footprinting evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001826 ultraviolet light footprinting evidence A type of ultraviolet light footprinting evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005670 x-ray crystallography evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001823 x-ray crystallography evidence A type of x-ray crystallography evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005671 x-ray crystallography evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007374 crystallography evidence used in automatic assertion A type of x-ray crystallography evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0005672 xylE reporter gene assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005537 xylE reporter gene assay evidence A type of xylE reporter gene assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005673 ad-hoc qualitative phenotype observation evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence where a non-standard trait (e.g. natural competence) is assessed qualitatively (e.g. presence/absence) and used as a natural reporter to conclude that some genes or pathways are regulated by the transcription factor. http://purl.obolibrary.org/obo/ECO_0005674 ad-hoc qualitative phenotype observation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005673 ad-hoc qualitative phenotype observation evidence A type of ad-hoc qualitative phenotype observation that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005675 ad-hoc quantitative phenotype observation evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence where a quantifiable phenotypic trait (e.g. glucose intake) is assessed in a quantitative manner after induction of the regulatory mechanism and used as a natural reporter to conclude that some genes or pathways are regulated by the transcription factor. http://purl.obolibrary.org/obo/ECO_0005676 ad-hoc quantitative phenotype observation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005675 ad-hoc quantitative phenotype observation evidence A type of ad-hoc quantitative phenotype observation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005800 dilution assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where the activity of a solution is determined by exposing an indicator culture or organism to a series of dilutions and determining the lowest concentration that elicits a biological response. http://purl.obolibrary.org/obo/ECO_0005801 enzymatic activity assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of enzyme assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005802 cell transfection experiment evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005606 cell transfection experiment evidence A type of cell transfection experiment evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005803 motility assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where the motility of a cell or cells is determined. http://purl.obolibrary.org/obo/ECO_0005804 immunofluorescence evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of immunofluorescence evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005805 yeast 2-hybrid evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000068 yeast 2-hybrid evidence A type of yeast 2-hybrid evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006001 Cya fusion reporter assay evidence http://purl.obolibrary.org/obo/ECO_0000049 reporter gene assay evidence A type of evidence derived from exploiting the CyaA (calmodulin-activated adenylate cyclase toxin) secreted by Bordatella pertussis to synthesize cAMP and alter cellular physiology in a host cell for various biological applications. http://purl.obolibrary.org/obo/ECO_0006002 Cya fusion reporter assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006001 Cya fusion reporter assay evidence A type of Cya fusion reporter assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006003 electron microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005033 electron microscopy evidence A type of electron microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006004 super-resolution microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005578 super-resolution microscopy evidence A type of super-resolution microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006005 fractionation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of fractionation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006006 electrophysiology assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of electrophysiology assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006007 chromatin immunoprecipitation-chip evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005644 immunoprecipitation evidence used in manual assertion A type of chromatin immunoprecipitation-chip evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006008 chromatin immunoprecipitation- exonuclease evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005644 immunoprecipitation evidence used in manual assertion A type of chromatin immunoprecipitation- exonuclease evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006009 chromatin immunoprecipitation-seq evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005644 immunoprecipitation evidence used in manual assertion A type of chromatin immunoprecipitation-seq evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006010 mRNA interactome capture evidence http://purl.obolibrary.org/obo/ECO_0000136 nucleic acid binding evidence A type of nucleic acid binding evidence in which RNA-binding proteins are identified through cross-linking RNA and proteins by UV light, capturing RNA-protein complexes on oligo(dT) beads, and finally identifying by mass spectrometry. http://purl.obolibrary.org/obo/ECO_0006011 mRNA interactome capture evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006010 mRNA interactome capture evidence A type of mRNA interactome capture evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006012 patch-clamp recording evidence http://purl.obolibrary.org/obo/ECO_0000164 electrophysiology assay evidence A type of electrophysiology assay evidence in which a glass micropipette is sealed to the surface of a cell membrane (patch) to study ion channels. http://purl.obolibrary.org/obo/ECO_0006013 patch-clamp recording evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006012 patch-clamp recording evidence A type of patch-clamp recording evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006014 whole-cell patch-clamp recording evidence http://purl.obolibrary.org/obo/ECO_0006012 patch-clamp recording evidence A type of patch-clamp recording evidence in which a glass micropipette filled with a prepared solution is used to form a seal with the cell membrane followed by rupture of membrane to provide accurate and high resolution electrical property measurements of the whole cell. http://purl.obolibrary.org/obo/ECO_0006015 whole-cell patch-clamp recording evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006014 whole-cell patch-clamp recording evidence A type of whole-cell patch-clamp recording evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006016 author statement supported from referenced clinical study http://purl.obolibrary.org/obo/ECO_0000033 author statement supported by traceable reference A type of author statement supported by traceable reference in which the traceable reference is a clinical study. http://purl.obolibrary.org/obo/ECO_0006017 author statement supported from referenced clinical study used in manual assertion http://purl.obolibrary.org/obo/ECO_0006016 author statement supported from referenced clinical study A type of author statement supported from referenced clinical study that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006018 inference based on individual clinical experience http://purl.obolibrary.org/obo/ECO_0000205 curator inference A type of curator inference that is based on the individual clinical experience of a clinician http://purl.obolibrary.org/obo/ECO_0006019 inference based on individual clinical experience used in manual assertion http://purl.obolibrary.org/obo/ECO_0006018 inference based on individual clinical experience A type of inference based on individual clinical experience that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006020 biofilm formation assay evidence http://purl.obolibrary.org/obo/ECO_0005007 cell proliferation assay evidence A type of cell proliferation assay evidence in which biofilm growth is monitored and detected from attachment to development. http://purl.obolibrary.org/obo/ECO_0006021 biofilm formation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006020 biofilm formation assay evidence A type of biofilm formation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006022 microtiter plate biofilm assay evidence http://purl.obolibrary.org/obo/ECO_0006020 biofilm formation assay evidence A type of biofilm formation assay evidence in which microtiter dishes or tubes are inoculated and incubated to promote biofilm formation, and then biofilms are detected by staining with crystal violet or safranin to observe phenotypes. http://purl.obolibrary.org/obo/ECO_0006023 microtiter plate biofilm assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006022 microtiter plate biofilm assay evidence A type of microtiter plate biofilm assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006024 air-liquid interface assay evidence http://purl.obolibrary.org/obo/ECO_0006020 biofilm formation assay evidence A type of biofilm formation assay evidence in which biofilm formation is analyzed, without staining, over 4 to 48 hours by growth on a tilted multiwell plate. http://purl.obolibrary.org/obo/ECO_0006025 air-liquid interface assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006024 air-liquid interface assay evidence A type of air-liquid interface assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006026 colony biofilm assay evidence http://purl.obolibrary.org/obo/ECO_0006020 biofilm formation assay evidence A type of biofilm formation assay evidence in which a colony is grown on a semipermeable membrane on an agar plate. The plate serves to supply nutrients and the semipermeable membrane can be relocated to fresh plates. http://purl.obolibrary.org/obo/ECO_0006027 colony biofilm assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006026 colony biofilm assay evidence A type of colony biofilm assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006028 Kadouri drip-fed biofilm assay evidence http://purl.obolibrary.org/obo/ECO_0006020 biofilm formation assay evidence A type of biofilm formation assay evidence in which mature bacterial biofilms are grown in a multiwell plate that has a growth medium continually pumped through the wells while waste is continually pumped out. http://purl.obolibrary.org/obo/ECO_0006029 Kadouri drip-fed biofilm assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006028 Kadouri drip-fed biofilm assay evidence A type of Kadouri drip-fed biofilm assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006030 co-immunoprecipitation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005644 immunoprecipitation evidence used in manual assertion A type of co-immunoprecipitation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006031 immunolocalization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001232 microscopy evidence used in manual assertion A type of immunolocalization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006032 optogenetic evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence arising from experiment in which neuronal activity is manipulated using genetically encoded, optically activated neuronal actuators. http://purl.obolibrary.org/obo/ECO_0006033 optogenetic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006032 optogenetic evidence A type of optogenetic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006034 fluorescent sensor evidence http://purl.obolibrary.org/obo/ECO_0001115 fluorescence evidence A type of fluorescence evidence that is based on direct, quantitative measurement of some cellular property using a fluorescent sensor. http://purl.obolibrary.org/obo/ECO_0006035 fluorescent sensor evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006034 fluorescent sensor evidence A type of fluorescent sensor evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006036 genetically encoded fluorescent sensor evidence http://purl.obolibrary.org/obo/ECO_0006034 fluorescent sensor evidence A type of fluorescent sensor evidence that is based on direct, quantitative measurement of some cellular property using a genetically encoded fluorescent sensor. http://purl.obolibrary.org/obo/ECO_0006037 genetically encoded fluorescent sensor evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006036 genetically encoded fluorescent sensor evidence A type of genetically encoded fluorescent sensor evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006038 genetically encoded fluorescent electrophysiology assay evidence http://purl.obolibrary.org/obo/ECO_0006034 fluorescent sensor evidence A type of fluorescent sensor evidence and electrophysiology assay evidence where the electrical properties of cells or tissues are studied using fluorescent sensors. http://purl.obolibrary.org/obo/ECO_0006039 genetically encoded fluorescent electrophysiology assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006038 genetically encoded fluorescent electrophysiology assay evidence A type of genetically encoded fluorescent electrophysiology assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006040 genetically encoded fluorescent ion concentration sensor assay evidence http://purl.obolibrary.org/obo/ECO_0006038 genetically encoded fluorescent electrophysiology assay evidence A type of genetically encoded fluorescent electrophysiology assay evidence that is based on direct, quantitative measurement of ion concentration using a genetically encoded fluorescent sensor. http://purl.obolibrary.org/obo/ECO_0006041 genetically encoded fluorescent ion concentration sensor assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006040 genetically encoded fluorescent ion concentration sensor assay evidence A type of genetically encoded fluorescent ion concentration sensor assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007000 high throughput mutant phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of high throughput evidence derived from high throughput analysis of differences in alleles of a corresponding gene. http://purl.obolibrary.org/obo/ECO_0007001 high throughput mutant phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007000 high throughput mutant phenotypic evidence A type of high throughput mutant phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007002 high throughput genetic interaction phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000011 genetic interaction evidence A type of high throughput evidence resulting from the high throughput analysis of the effect that a given gene has on another gene or genes, and products. http://purl.obolibrary.org/obo/ECO_0007003 high throughput genetic interaction phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007002 high throughput genetic interaction phenotypic evidence A type of high throughput genetic interaction phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007004 high throughput direct assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of high throughput evidence derived from the high throughput direct measurement of some aspect of a biological feature. http://purl.obolibrary.org/obo/ECO_0007005 high throughput direct assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007004 high throughput direct assay evidence A type of high throughput evidence that is used in a manual assertion derived from the high throughput direct measurement of some aspect of a biological feature. http://purl.obolibrary.org/obo/ECO_0007006 high throughput expression pattern evidence http://purl.obolibrary.org/obo/ECO_0000008 expression pattern evidence A type of high throughput evidence derived from the high throughput characterization of gene expression. http://purl.obolibrary.org/obo/ECO_0007007 high throughput expression pattern evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007006 high throughput expression pattern evidence A type of high throughput evidence that is used in a manual assertion derived from the high throughput characterization of gene expression. http://purl.obolibrary.org/obo/OBI_0002444 measurand role http://purl.obolibrary.org/obo/BFO_0000023 role A role borne by a material entity and realized in an assay which achieves the objective to measure the magnitude/concentration/amount of the measurand in the entity bearing evaluant role. http://purl.obolibrary.org/obo/OBI_0002447 suppression subtractive hybridization http://purl.obolibrary.org/obo/OBI_0000253 artificially induced nucleic acid hybridization An artificially induced nucleic acid hybridization that is performed to compare gene expression in different cell or tissue types based on normalization and suppression, which creates a subtracted cDNA or genomic DNA library. http://purl.obolibrary.org/obo/OBI_0002448 differential screening hybridization http://purl.obolibrary.org/obo/OBI_0000253 artificially induced nucleic acid hybridization An artificially induced nucleic acid hybridization that is performed to identify differentially expressed genes through hybridization of cDNA probes. http://purl.obolibrary.org/obo/ECO_0001533 localization evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence in which the sub-cellular location of a protein or nucleic acid sequence is determined. http://purl.obolibrary.org/obo/ECO_0001534 nucleic acid localization evidence http://purl.obolibrary.org/obo/ECO_0001533 localization evidence A type of localization evidence based on localization of a specific segment of DNA or RNA within tissue by the application of a complementary strand of nucleic acid to which a reporter molecule (i.e. either radio-, fluorescent- or antigen-labeled probe) is attached and quantified using either autoradiography, fluorescence microscopy, or immunohistochemistry. http://purl.obolibrary.org/obo/ECO_0007089 loss-of-function mutant phenotype evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000315 mutant phenotype evidence used in manual assertion A type of loss-of-function mutant phenotype evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007090 structural similarity evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000252 similarity evidence used in manual assertion A type of structural similarity evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007092 gain-of-function mutant phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000315 mutant phenotype evidence used in manual assertion A type of gain-of-function mutant phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007093 voucher specimen phenotypic analysis evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000050 voucher specimen phenotypic analysis evidence A type of voucher specimen phenotypic analysis evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007094 positional similarity evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000252 similarity evidence used in manual assertion A type of positional similarity evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007095 quantitative trait analysis evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000061 quantitative trait analysis evidence A type of quantitative trait analysis evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007096 compositional similarity evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000252 similarity evidence used in manual assertion A type of compositional similarity evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007097 developmental similarity evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000252 similarity evidence used in manual assertion A type of developmental similarity evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007098 morphological similarity evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000252 similarity evidence used in manual assertion A type of morphological similarity evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007099 gene expression similarity evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000252 similarity evidence used in manual assertion A type of gene expression similarity evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007100 methylation-specific polymerase chain reaction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001198 in vitro methylation assay evidence used in manual assertion A type of methylation-specific polymerase chain reaction evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007101 southern hybridization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of southern hybridization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007102 intermethylated site amplification evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001198 in vitro methylation assay evidence used in manual assertion A type of intermethylated site amplification evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007103 epitope-tagged protein immunolocalization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006031 immunolocalization evidence used in manual assertion A type of epitope-tagged protein immunolocalization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007104 co-fractionation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006005 fractionation evidence used in manual assertion A type of co-fractionation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007106 green fluorescent protein fusion protein localization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of green fluorescent protein fusion protein localization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007107 yellow fluorescent protein fusion protein localization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of yellow fluorescent protein fusion protein localization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007108 beta-glucuronidase fusion protein localization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of beta-glucuronidase fusion protein localization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007109 beta-galactosidase fusion protein localization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of beta-galactosidase fusion protein localization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007110 thin layer chromatography evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007680 chromatography evidence used in manual assertion A type of thin layer chromatography evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007111 in vitro recombinant protein transcription reconstitution assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of in vitro recombinant protein transcription reconstitution assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007112 protein separation followed by direct sequencing evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006005 fractionation evidence used in manual assertion A type of protein separation followed by direct sequencing evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007113 protein separation followed by fragment identification evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006005 fractionation evidence used in manual assertion A type of protein separation followed by fragment identification evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007114 heterologous system uptake evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of heterologous system uptake evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007115 two-electrode voltage clamp recording evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006006 electrophysiology assay evidence used in manual assertion A type of two-electrode voltage clamp recording evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007116 biochemical trait analysis evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000172 biochemical trait analysis evidence A type of biochemical trait analysis evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007117 mutant physiological response evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000315 mutant phenotype evidence used in manual assertion A type of mutant physiological response evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007118 mutant visible phenotype evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000315 mutant phenotype evidence used in manual assertion A type of mutant visible phenotype evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007119 in vivo assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of in vivo assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007120 animal model system study evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of animal model system study evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007121 clinical study evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of clinical study evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007122 in vitro assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of in vitro assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007123 enzyme inhibition evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005801 enzymatic activity assay evidence used in manual assertion A type of enzyme inhibition evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007125 Illumina sequencing evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of Illumina sequencing evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007126 454 pyrosequencing evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of 454 pyrosequencing evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007127 SOLiD sequencing evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of SOLiD sequencing evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007128 chain termination sequencing evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of chain termination sequencing evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007129 chromatin immunoprecipitation-qPCR evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005644 immunoprecipitation evidence used in manual assertion A type of chromatin immunoprecipitation-qPCR evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007130 4C evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of 4C evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007131 5C evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of 5C evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007133 3C-qPCR evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of 3C-qPCR evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007134 Hi-C evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of Hi-C evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007135 3C-seq evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000269 experimental evidence used in manual assertion A type of 3C-seq evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007136 environmental perturbation phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000241 environmental perturbation phenotypic evidence A type of environmental perturbation phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007137 tissue ablation phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000242 tissue ablation phenotypic evidence A type of tissue ablation phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007138 tissue grafting phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000243 tissue grafting phenotypic evidence A type of tissue grafting phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007141 cytochalasin experiment evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000299 cytochalasin experiment evidence A type of cytochalasin experiment evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007142 green fluorescent protein immunolocalization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006031 immunolocalization evidence used in manual assertion A type of green fluorescent protein immunolocalization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007143 beta-galactosidase protein immunolocalization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006031 immunolocalization evidence used in manual assertion A type of beta-galactosidase protein immunolocalization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007144 cap analysis of gene expression evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000309 cap analysis of gene expression evidence A type of cap analysis of gene expression evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007145 nano-cap analysis of gene expression evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000310 nano-cap analysis of gene expression evidence A type of nano-cap analysis of gene expression evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007146 particle size and count assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000334 particle size and count assay evidence A type of particle size and count assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007147 competitive growth assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000336 competitive growth assay evidence A type of competitive growth assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007148 pulsed-field gel electrophoresis evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000338 pulsed-field gel electrophoresis evidence A type of pulsed-field gel electrophoresis evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007149 two-dimensional agarose gel electrophoresis evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000339 two-dimensional agarose gel electrophoresis evidence A type of two-dimensional agarose gel electrophoresis evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007150 plasmid maintenance assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000340 plasmid maintenance assay evidence A type of plasmid maintenance assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007151 specific protein inhibition by antibody evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000341 specific protein inhibition by antibody evidence A type of specific protein inhibition by antibody evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007152 single exon transcript confirmation via alignment evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000345 single exon transcript confirmation via alignment evidence A type of single exon transcript confirmation via alignment evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007153 phylogenetic distribution evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000355 phylogenetic distribution evidence A type of phylogenetic distribution evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007154 differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) used in manual assertion http://purl.obolibrary.org/obo/ECO_0000358 differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) A type of differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007155 differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) used in manual assertion http://purl.obolibrary.org/obo/ECO_0000359 differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) A type of differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007156 biological target-disease association via drug evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000360 biological target-disease association via drug evidence A type of biological target-disease association via drug that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007157 cell staining evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001603 cell staining evidence A type of cell staining evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007159 ATP bioluminescence assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005011 ATP bioluminescence assay evidence A type of ATP bioluminescence assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007160 missense mutation pohenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005506 missense mutation phenotypic evidence A type of missense mutation phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007161 nonsense mutation phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005507 nonsense mutation phenotypic evidence A type of nonsense mutation phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007162 silent mutation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005508 silent mutation evidence A type of silent mutation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007163 insertion mutation phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005509 insertion mutation phenotypic evidence A type of insertion mutation phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007164 duplication mutation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005511 duplication mutation evidence A type of duplication mutation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007165 frameshift mutation phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005512 frameshift mutation phenotypic evidence A type of frameshift mutation phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007166 repeat expansion mutation phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005513 repeat expansion mutation phenotypic evidence A type of repeat expansion mutation phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007167 splice site mutation phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005514 splice site mutation phenotypic evidence A type of splice site mutation phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007168 translocation mutation phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005515 translocation mutation phenotypic evidence A type of translocation mutation phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007169 in-gel protein kinase assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005801 enzymatic activity assay evidence used in manual assertion A type of in-gel protein kinase assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007170 macroscopic current trace evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006006 electrophysiology assay evidence used in manual assertion A type of macroscopic current trace evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007171 current density evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006006 electrophysiology assay evidence used in manual assertion A type of current density evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007172 sustained current evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006006 electrophysiology assay evidence used in manual assertion A type of sustained current evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007173 use dependence of inactivation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006006 electrophysiology assay evidence used in manual assertion A type of use dependence of inactivation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007174 current clamp recording evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006006 electrophysiology assay evidence used in manual assertion A type of current clamp recording evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007175 whole-cell voltage clamp recording evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006006 electrophysiology assay evidence used in manual assertion A type of whole-cell voltage clamp recording evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007176 cell-attached single-channel recording evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006013 patch-clamp recording evidence used in manual assertion A type of cell-attached single-channel recording evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007177 cell-detached inside-out single-channel recording evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006013 patch-clamp recording evidence used in manual assertion A type of cell-detached inside-out single-channel recording evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007178 reconstituted bilayer single-channel patch recording evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006013 patch-clamp recording evidence used in manual assertion A type of reconstituted bilayer single-channel patch recording evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007179 electroencephalography recording evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006006 electrophysiology assay evidence used in manual assertion A type of electroencephalography recording evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007180 cell-detached outside-out single-channel recording evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006013 patch-clamp recording evidence used in manual assertion A type of cell-detached outside-out single-channel recording evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007181 cut-open oocyte voltage clamp recording evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006006 electrophysiology assay evidence used in manual assertion A type of cut-open oocyte voltage clamp recording evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007182 macropatch voltage clamp recording evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006006 electrophysiology assay evidence used in manual assertion A type of macropatch voltage clamp recording evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007184 protein mass spectrometry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005603 protein mass spectrometry evidence A type of protein mass spectrometry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007185 cross-streak test evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005604 cross-streak test evidence A type of cross-streak test evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007186 tethered cell assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005607 tethered cell assay evidence A type of tethered cell assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007187 tumble frequency assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005608 tumble frequency assay evidence A type of tumble frequency assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007188 capillary assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005609 capillary assay evidence A type of capillary assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007191 inference by association of genotype from phenotype used in manual assertion http://purl.obolibrary.org/obo/ECO_0001237 natural variation mutant evidence used in manual assertion A type of inference by association of genotype from phenotype that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007192 motility assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005803 motility assay evidence A type of motility assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007193 loss-of-function mutant phenotype evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of loss-of-function mutant phenotype evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007194 structural similarity evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000027 structural similarity evidence A type of structural similarity evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007196 gain-of-function mutant phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of gain-of-function mutant phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007197 voucher specimen phenotypic analysis evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000050 voucher specimen phenotypic analysis evidence A type of voucher specimen phenotypic analysis evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007198 positional similarity evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000060 positional similarity evidence A type of positional similarity evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007199 quantitative trait analysis evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000061 quantitative trait analysis evidence A type of quantitative trait analysis evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007200 compositional similarity evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000063 compositional similarity evidence A type of compositional similarity evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007201 developmental similarity evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000067 developmental similarity evidence A type of developmental similarity evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007202 morphological similarity evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000071 morphological similarity evidence A type of morphological similarity evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007203 gene expression similarity evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000075 gene expression similarity evidence A type of gene expression similarity evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007204 methylation-specific polymerase chain reaction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007401 in vitro methylation assay evidence used in automatic assertion A type of methylation-specific polymerase chain reaction evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007205 southern hybridization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000078 southern hybridization evidence A type of southern hybridization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007206 intermethylated site amplification evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007401 in vitro methylation assay evidence used in automatic assertion A type of intermethylated site amplification evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007207 epitope-tagged protein immunolocalization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007486 immunolocalization evidence used in automatic assertion A type of epitope-tagged protein immunolocalization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007208 co-fractionation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007534 fractionation evidence used in automatic assertion A type of co-fractionation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007210 green fluorescent protein fusion protein localization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000126 green fluorescent protein fusion protein localization evidence A type of green fluorescent protein fusion protein localization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007211 yellow fluorescent protein fusion protein localization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000128 yellow fluorescent protein fusion protein localization evidence A type of yellow fluorescent protein fusion protein localization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007212 beta-glucuronidase fusion protein localization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000130 beta-glucuronidase fusion protein localization evidence A type of beta-glucuronidase fusion protein localization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007213 beta-galactosidase fusion protein localization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000132 beta-galactosidase fusion protein localization evidence A type of beta-galactosidase fusion protein localization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007214 thin layer chromatography evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007681 chromatography evidence used in automatic assertion A type of thin layer chromatography evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007215 in vitro recombinant protein transcription reconstitution assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000152 in vitro recombinant protein transcription reconstitution assay evidence A type of in vitro recombinant protein transcription reconstitution assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007216 protein separation followed by direct sequencing evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000158 protein separation followed by direct sequencing evidence A type of protein separation followed by direct sequencing evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007217 protein separation followed by fragment identification evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000160 protein separation followed by fragment identification evidence A type of protein separation followed by fragment identification evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007218 heterologous system uptake evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000162 heterologous system uptake evidence A type of heterologous system uptake evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007219 two-electrode voltage clamp recording evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000166 two-electrode voltage clamp recording evidence A type of two-electrode voltage clamp recording evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007220 biochemical trait analysis evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000172 biochemical trait analysis evidence A type of biochemical trait analysis evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007221 mutant physiological response evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of mutant physiological response evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007222 mutant visible phenotype evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of mutant visible phenotype evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007223 in vivo assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of in vivo assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007224 animal model system study evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of animal model system study evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007225 clinical study evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of clinical study evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007226 in vitro assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of in vitro assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007227 enzyme inhibition evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007468 enzymatic activity assay evidence used in automatic assertion A type of enzyme inhibition evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007229 Illumina sequencing evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000222 Illumina sequencing evidence A type of Illumina sequencing evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007230 454 pyrosequencing evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000223 454 pyrosequencing evidence A type of 454 pyrosequencing evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007231 SOLiD sequencing evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000224 SOLiD sequencing evidence A type of SOLiD sequencing evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007232 chain termination sequencing evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000225 chain termination sequencing evidence A type of chain termination sequencing evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007233 chromatin immunoprecipitation-qPCR evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000228 chromatin immunoprecipitation-qPCR evidence A type of chromatin immunoprecipitation-qPCR evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007234 4C evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000234 4C evidence A type of 4C evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007235 5C evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000235 5C evidence A type of 5C evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007237 3C-qPCR evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000237 3C-qPCR evidence A type of 3C-qPCR evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007238 Hi-C evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000238 Hi-C evidence A type of Hi-C evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007239 3C-seq evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000239 3C-seq evidence A type of 3C-seq evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007240 environmental perturbation phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000241 environmental perturbation phenotypic evidence A type of environmental perturbation phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007241 tissue ablation phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000242 tissue ablation phenotypic evidence A type of tissue ablation phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007242 tissue grafting phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000243 tissue grafting phenotypic evidence A type of tissue grafting phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007245 cytochalasin experiment evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000299 cytochalasin experiment evidence A type of cytochalasin experiment evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007246 green fluorescent protein immunolocalization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007486 immunolocalization evidence used in automatic assertion A type of green fluorescent protein immunolocalization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007247 beta-galactosidase protein immunolocalization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007486 immunolocalization evidence used in automatic assertion A type of beta-galactosidase protein immunolocalization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007248 cap analysis of gene expression evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007303 transcript expression evidence used in automatic assertion A type of cap analysis of gene expression evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007249 nano-cap analysis of gene expression evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007303 transcript expression evidence used in automatic assertion A type of nano-cap analysis of gene expression evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007250 particle size and count assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of particle size and count assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007251 competitive growth assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000336 competitive growth assay evidence A type of competitive growth assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007252 pulsed-field gel electrophoresis evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000338 pulsed-field gel electrophoresis evidence A type of pulsed-field gel electrophoresis evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007253 two-dimensional agarose gel electrophoresis evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000339 two-dimensional agarose gel electrophoresis evidence A type of two-dimensional agarose gel electrophoresis evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007254 plasmid maintenance assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of plasmid maintenance assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007255 specific protein inhibition by antibody evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000341 specific protein inhibition by antibody evidence A type of specific protein inhibition by antibody evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007256 single exon transcript confirmation via alignment evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000345 single exon transcript confirmation via alignment evidence A type of single exon transcript confirmation via alignment evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007257 phylogenetic distribution evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000355 phylogenetic distribution evidence A type of phylogenetic distribution evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007258 differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007303 transcript expression evidence used in automatic assertion A type of differential geneset expression evidence from microarray experiment (GSEA, Fisher-exact) that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007259 differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007303 transcript expression evidence used in automatic assertion A type of differential geneset expression evidence from RNA-seq experiment (GSEA, Fisher-exact) that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007260 biological target-disease association via drug evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of biological target-disease association via drug that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007261 cell staining evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001603 cell staining evidence A type of cell staining evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007263 ATP bioluminescence assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005011 ATP bioluminescence assay evidence A type of ATP bioluminescence assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007264 missense mutation phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007448 point mutation phenotypic evidence used in automatic assertion A type of missense mutation phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007265 nonsense mutation phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007448 point mutation phenotypic evidence used in automatic assertion A type of nonsense mutation phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007266 silent mutation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007448 point mutation phenotypic evidence used in automatic assertion A type of silent mutation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007267 insertion mutation phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of insertion mutation phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007268 duplication mutation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005511 duplication mutation evidence A type of duplication mutation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007269 frameshift mutation phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of frameshift mutation phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007270 repeat expansion mutation phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of repeat expansion mutation phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007271 splice site mutation phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of splice site mutation phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007272 translocation mutation phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of translocation mutation phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007273 in-gel protein kinase assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005562 in-gel protein kinase assay evidence A type of in-gel protein kinase assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007274 macroscopic current trace evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005563 macroscopic current trace evidence A type of macroscopic current trace evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007275 current density evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007535 electrophysiology assay evidence used in automatic assertion A type of current density evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007276 sustained current evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007535 electrophysiology assay evidence used in automatic assertion A type of sustained current evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007277 use dependence of inactivation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007535 electrophysiology assay evidence used in automatic assertion A type of use dependence of inactivation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007278 current clamp recording evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007535 electrophysiology assay evidence used in automatic assertion A type of current clamp recording evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007279 whole-cell voltage clamp recording evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005572 whole-cell voltage clamp recording evidence A type of whole-cell voltage clamp recording evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007280 cell-attached single-channel recording evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007537 patch-clamp recording evidence used in automatic assertion A type of cell-attached single-channel recording evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007281 cell-detached inside-out single-channel recording evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007537 patch-clamp recording evidence used in automatic assertion A type of cell-detached inside-out single-channel recording evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007282 reconstituted bilayer single-channel patch recording evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007537 patch-clamp recording evidence used in automatic assertion A type of reconstituted bilayer single-channel patch recording evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007283 electroencephalography recording evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007535 electrophysiology assay evidence used in automatic assertion A type of electroencephalography recording evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007284 cell-detached outside-out single-channel recording evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007537 patch-clamp recording evidence used in automatic assertion A type of cell-detached outside-out single-channel recording evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007285 cut-open oocyte voltage clamp recording evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005583 cut-open oocyte voltage clamp recording evidence A type of cut-open oocyte voltage clamp recording evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007286 macropatch voltage clamp recording evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005584 macropatch voltage clamp recording evidence A type of macropatch voltage clamp recording evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007288 protein mass spectrometry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007433 mass spectrometry evidence used in automatic assertion A type of protein mass spectrometry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007289 cross-streak test evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005604 cross-streak test evidence A type of cross-streak test evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007290 tethered cell assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005607 tethered cell assay evidence A type of tethered cell assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007291 tumble frequency assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005608 tumble frequency assay evidence A type of tumble frequency assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007292 capillary assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005609 capillary assay evidence A type of capillary assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007295 inference by association of genotype from phenotype used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007440 natural variation mutant evidence used in automatic assertion A type of inference by association of genotype from phenotype that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007296 motility assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of motility assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000501 evidence used in automatic assertion A type of experimental evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007298 expression pattern evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of expression pattern evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007299 Affymetrix GeneChip evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007300 cRNA to DNA expression microarray evidence used in automatic assertion A type of Affymetrix GeneChip evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007300 cRNA to DNA expression microarray evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007301 expression microarray evidence used in automatic assertion A type of cRNA to DNA expression microarray evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007301 expression microarray evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007303 transcript expression evidence used in automatic assertion A type of expression microarray evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007302 differential methylation hybridization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007401 in vitro methylation assay evidence used in automatic assertion A type of differential methylation hybridization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007303 transcript expression evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007298 expression pattern evidence used in automatic assertion A type of transcript expression evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007304 Nimblegen array evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005630 cDNA to DNA expression microarray evidence used in automatic assertion A type of Nimblegen array evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007305 array-based sequence capture evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000093 array-based sequence capture evidence A type of array-based sequence capture evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007306 qualitative western immunoblotting evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007309 protein expression evidence used in automatic assertion A type of qualitative western immunoblotting evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of direct assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007309 protein expression evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007298 expression pattern evidence used in automatic assertion A type of protein expression evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007310 expression library screen evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007309 protein expression evidence used in automatic assertion A type of expression library screen evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007311 heterologous protein expression evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007309 protein expression evidence used in automatic assertion A type of heterologous protein expression evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007312 spatial pattern of protein expression evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007309 protein expression evidence used in automatic assertion A type of spatial pattern of protein expression evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007313 DNA to cDNA expression microarray evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007301 expression microarray evidence used in automatic assertion A type of DNA to cDNA expression microarray evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007314 differential hybridization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007303 transcript expression evidence used in automatic assertion A type of differential hybridization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007315 RNA protection assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007316 nuclease protection assay evidence used in automatic assertion A type of RNA protection assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007316 nuclease protection assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001106 nuclease protection assay evidence A type of nuclease protection assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007317 spatial pattern of transcript expression evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007303 transcript expression evidence used in automatic assertion A type of spatial pattern of transcript expression evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007318 subtractive hybridization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007303 transcript expression evidence used in automatic assertion A type of subtractive hybridization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007319 author statement used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000204 author statement A type of author statement that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007320 author statement without traceable support used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007319 author statement used in automatic assertion A type of author statement without traceable support that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007321 author statement supported by traceable reference used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007319 author statement used in automatic assertion A type of author statement supported by traceable reference that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007322 curator inference used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000205 curator inference A type of curator inference that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007323 inference from background scientific knowledge used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007322 curator inference used in automatic assertion A type of inference from background scientific knowledge that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007324 no evidence data found used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007322 curator inference used in automatic assertion An inference that results when research finds no evidence information in the scientific literature, at reference databases, or from other resources that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000015 mutant phenotype evidence A type of mutant phenotype evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007326 genetic interaction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000011 genetic interaction evidence A type of genetic interaction evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007327 genomic context evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000251 similarity evidence used in automatic assertion A type of genomic context evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007328 biological aspect of ancestor evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000308 biological aspect of ancestor evidence A type of biological aspect of ancestor evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007329 biological aspect of descendant evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000214 biological aspect of descendant evidence A type of biological aspect of descendant evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007330 phylogenetic determination of loss of key residues evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000216 phylogenetic determination of loss of key residues evidence A type of phylogenetic determination of loss of key residues evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007331 rapid divergence from ancestral sequence evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000215 rapid divergence from ancestral sequence evidence A type of rapid divergence from ancestral sequence evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007332 physical interaction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of physical interaction evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007333 gene neighbors evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007327 genomic context evidence used in automatic assertion A type of gene neighbors evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007334 Edman degradation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001044 Edman degradation evidence A type of Edman degradation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007335 3D cell culture evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001001 3D cell culture evidence A type of 3D cell culture evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007336 51Cr release assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001004 51Cr release assay evidence A type of 51Cr release assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007337 7-aminoactinomycin staining evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001005 7-aminoactinomycin staining evidence A type of 7-aminoactinomycin staining evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007338 [3H]-thymidine incorporation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001003 [3H]-thymidine incorporation assay evidence A type of [3H]-thymidine incorporation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007339 [3H]arachidonic acid release assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001002 [3H]arachidonic acid release assay evidence A type of [3H]arachidonic acid release assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007340 adhesion assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001006 adhesion assay evidence A type of adhesion assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007341 adoptive cell transfer evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001007 adoptive cell transfer evidence A type of adoptive cell transfer evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007342 alamarBlue assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001008 alamarBlue assay evidence A type of alamarBlue assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007343 allograft transplantation phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001009 allograft transplantation phenotypic evidence A type of allograft transplantation phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007344 anion-exchange chromatography evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007681 chromatography evidence used in automatic assertion A type of anion-exchange chromatography evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007345 annexin-V staining evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001011 annexin-V staining evidence A type of annexin-V staining evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007346 cognitive assay phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001012 cognitive assay phenotypic evidence A type of cognitive assay phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007347 blocking monoclonal antibody evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007348 immunological assay evidence used in automatic assertion A type of blocking monoclonal antibody evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007348 immunological assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007384 affinity evidence used in automatic assertion A type of immunological assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007349 blocking peptide evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007348 immunological assay evidence used in automatic assertion A type of blocking peptide evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007350 blocking polyclonal antibody evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007348 immunological assay evidence used in automatic assertion A type of blocking polyclonal antibody evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007351 blood test evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of blood test evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007352 Boyden chamber assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001017 Boyden chamber assay evidence A type of Boyden chamber assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007353 bromodeoxyuridine incorporation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007354 nucleotide analog incorporation assay evidence used in automatic assertion A type of bromodeoxyuridine incorporation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007354 nucleotide analog incorporation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001107 nucleotide analog incorporation assay evidence A type of nucleotide analog incorporation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007355 caspase assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001019 caspase assay evidence A type of caspase assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007356 cell counting evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001020 cell counting evidence A type of cell counting evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007357 cell permeability assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001021 cell permeability assay evidence A type of cell permeability assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007358 carboxyfluorescein diacetate succinimidyl ester staining evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001022 carboxyfluorescein diacetate succinimidyl ester staining evidence A type of carboxyfluorescein diacetate succinimidyl ester staining evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007359 chemiluminescence-linked immunoassay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001023 chemiluminescence-linked immunoassay evidence A type of chemiluminescence-linked immunoassay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007360 chimeric protein phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of chimeric protein phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007361 co-electrophoresis evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001025 co-electrophoresis evidence A type of co-electrophoresis evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007362 co-localization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007363 imaging assay evidence used in automatic assertion A type of co-localization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007363 imaging assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of imaging assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007364 co-sedimentation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007332 physical interaction evidence used in automatic assertion A type of co-sedimentation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007365 colony counting evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of colony counting evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007366 comet assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001029 comet assay evidence A type of comet assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007367 conditional knockin evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007368 knockin evidence used in automatic assertion A type of conditional knockin evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007368 knockin evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007427 genetic transformation evidence used in automatic assertion A type of knockin evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007369 conditional knockout evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007370 knockout evidence used in automatic assertion A type of conditional knockout evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007370 knockout evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007427 genetic transformation evidence used in automatic assertion A type of knockout evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007371 constitutively active mutant protein evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of constitutively active mutant protein evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007372 cross-linking evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007373 protein binding evidence used in automatic assertion A type of cross-linking evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007373 protein binding evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007384 affinity evidence used in automatic assertion A type of protein binding evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007374 crystallography evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001034 crystallography evidence A type of crystallography evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007375 cytochemistry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007376 histochemistry evidence used in automatic assertion A type of cytochemistry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007376 histochemistry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007389 histology evidence used in automatic assertion A type of histochemistry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007377 cytochrome C release assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001036 cytochrome C release assay evidence A type of cytochrome C release assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007378 4',6-diamidino-2-phenylindole staining evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001037 4',6-diamidino-2-phenylindole staining evidence A type of 4',6-diamidino-2-phenylindole staining evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007379 deletion mutation phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of deletion mutation phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007380 DNA laddering assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001039 DNA laddering assay evidence A type of DNA laddering assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007381 RNA dot blot assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001040 RNA dot blot assay evidence A type of RNA dot blot assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007382 dominant-negative mutant phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of dominant-negative mutant phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007383 eTag assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007384 affinity evidence used in automatic assertion A type of eTag assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007384 affinity evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007332 physical interaction evidence used in automatic assertion A type of affinity evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007385 filter binding assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007332 physical interaction evidence used in automatic assertion A type of filter binding assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007386 fluorescence in situ hybridization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001837 in situ hybridization evidence used in automatic assertion A type of fluorescence in situ hybridization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007387 fluorescence resonance energy transfer evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001048 fluorescence resonance energy transfer evidence A type of fluorescence resonance energy transfer evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007388 gel-filtration evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007681 chromatography evidence used in automatic assertion A type of gel-filtration evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007389 histology evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007363 imaging assay evidence used in automatic assertion A type of histology evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007390 immunocytochemistry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001053 immunocytochemistry evidence A type of immunocytochemistry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007391 immunodepletion evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007348 immunological assay evidence used in automatic assertion A type of immunodepletion evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007392 immunohistochemistry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001055 immunohistochemistry evidence A type of immunohistochemistry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007393 in vitro acetylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001057 in vitro acetylation assay evidence A type of in vitro acetylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007394 in vitro cleavage assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001058 in vitro cleavage assay evidence A type of in vitro cleavage assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007395 in vitro deacetylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001060 in vitro deacetylation assay evidence A type of in vitro deacetylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007396 in vitro defarnesylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001061 in vitro defarnesylation assay evidence A type of in vitro defarnesylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007397 in vitro demethylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001062 in vitro demethylation assay evidence A type of in vitro demethylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007398 in vitro desumoylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001063 in vitro desumoylation assay evidence A type of in vitro desumoylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007399 in vitro deubiquitination assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001059 in vitro deubiquitination assay evidence A type of in vitro deubiquitination assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007400 in vitro farnesylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001064 in vitro farnesylation assay evidence A type of in vitro farnesylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007401 in vitro methylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001065 in vitro methylation assay evidence A type of in vitro methylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007402 in vitro palmitoylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001066 in vitro palmitoylation assay evidence A type of in vitro palmitoylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007403 in vitro phosphatase assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001067 in vitro phosphatase assay evidence A type of in vitro phosphatase assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007404 in vitro polyADP-ribosylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001069 in vitro polyADP-ribosylation assay evidence A type of in vitro polyADP-ribosylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007405 in vitro protein kinase assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001068 in vitro protein kinase assay evidence A type of in vitro protein kinase assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007406 in vitro sumoylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001070 in vitro sumoylation assay evidence A type of in vitro sumoylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007407 in vitro transcription assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001071 in vitro transcription assay evidence A type of in vitro transcription assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007408 in vitro translation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001072 in vitro translation assay evidence A type of in vitro translation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007409 in vitro ubiquitination assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001073 in vitro ubiquitination assay evidence A type of in vitro ubiquitination assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007410 in vivo acetylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001074 in vivo acetylation assay evidence A type of in vivo acetylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007411 in vivo cleavage assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001075 in vivo cleavage assay evidence A type of in vivo cleavage assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007412 in vivo deacetylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001076 in vivo deacetylation assay evidence A type of in vivo deacetylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007413 in vivo defarnesylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001077 in vivo defarnesylation assay evidence A type of in vivo defarnesylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007414 in vivo demethylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001078 in vivo demethylation assay evidence A type of in vivo demethylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007415 in vivo desumoylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001080 in vivo desumoylation assay evidence A type of in vivo desumoylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007416 in vivo deubiquitination assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001079 in vivo deubiquitination assay evidence A type of in vivo deubiquitination assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007417 in vivo farnesylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001081 in vivo farnesylation assay evidence A type of in vivo farnesylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007418 in vivo methylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001082 in vivo methylation assay evidence A type of in vivo methylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007419 in vivo palmitoylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001083 in vivo palmitoylation assay evidence A type of in vivo palmitoylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007420 in vivo phosphatase assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001084 in vivo phosphatase assay evidence A type of in vivo phosphatase assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007421 in vivo protein kinase assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001085 in vivo protein kinase assay evidence A type of in vivo protein kinase assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007422 in vivo sumoylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001086 in vivo sumoylation assay evidence A type of in vivo sumoylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007423 in vivo transcription assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001087 in vivo transcription assay evidence A type of in vivo transcription assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007424 in vivo translation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001088 in vivo translation assay evidence A type of in vivo translation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007425 in vivo ubiquitination assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001089 in vivo ubiquitination assay evidence A type of in vivo ubiquitination assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007427 genetic transformation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of genetic transformation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007428 lipid binding assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007332 physical interaction evidence used in automatic assertion A type of lipid binding assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007429 luminescence-based mammalian interactome mapping assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007332 physical interaction evidence used in automatic assertion A type of luminescence-based mammalian interactome mapping assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007430 macroscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of macroscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007431 mammalian 2-hybrid assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007432 bait-prey hybrid interaction evidence used in automatic assertion A type of mammalian 2-hybrid assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007432 bait-prey hybrid interaction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006393 bait-prey interaction evidence used in automatic assertion A type of bait-prey hybrid interaction evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007433 mass spectrometry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001096 mass spectrometry evidence A type of mass spectrometry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007434 medical imaging evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007363 imaging assay evidence used in automatic assertion A type of medical imaging evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007435 microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007363 imaging assay evidence used in automatic assertion A type of microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007436 motility wound healing assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001099 motility wound healing assay evidence A type of motility wound healing assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007437 MTS assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001100 MTS assay evidence A type of MTS assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007438 MTT assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001101 MTT assay evidence A type of MTT assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007439 multiplex bead-based immunoassay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001102 multiplex bead-based immunoassay evidence A type of multiplex bead-based immunoassay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007440 natural variation mutant evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001103 natural variation mutant evidence A type of natural variation mutant evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007441 nuclear magnetic resonance evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001105 nuclear magnetic resonance evidence A type of nuclear magnetic resonance evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007442 nuclear fragmentation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001104 nuclear fragmentation evidence A type of nuclear fragmentation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007443 phage display evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007373 protein binding evidence used in automatic assertion A type of phage display evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007444 phosphoamino acid analysis evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of phosphoamino acid analysis evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007445 peptide affinity enrichment evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007384 affinity evidence used in automatic assertion A type of peptide affinity enrichment evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007446 peptide array evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007373 protein binding evidence used in automatic assertion A type of peptide array evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007447 physical examination evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of physical examination evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007448 point mutation phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of point mutation phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007449 propidium iodide staining evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001114 propidium iodide staining evidence A type of propidium iodide staining evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007450 fluorescence evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of fluorescence evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007451 protein dot blot assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001116 protein dot blot assay evidence A type of protein dot blot assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007452 protein microarray evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001117 protein microarray evidence A type of protein microarray evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007453 protein sequencing assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001118 protein sequencing assay evidence A type of protein sequencing assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007454 quantitative mass spectrometry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007433 mass spectrometry evidence used in automatic assertion A type of quantitative mass spectrometry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007455 radioisotope assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of radioisotope assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007456 radioimmunoassay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001121 radioimmunoassay evidence A type of radioimmunoassay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007457 restriction fragment detection evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001124 restriction fragment detection evidence A type of restriction fragment detection evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007458 spectrophotometry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001126 spectrophotometry evidence A type of spectrophotometry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007459 syngeneic transplantation experiment evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001128 syngeneic transplantation experiment evidence A type of syngeneic transplantation experiment evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007460 xenotransplantation phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001137 xenotransplantation phenotypic evidence A type of xenotransplantation phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007461 WST-1 assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001136 WST-1 assay evidence A type of WST-1 assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007462 urine test evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of urine test evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007463 terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001133 terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence A type of terminal deoxynucleotidyl transferase dUTP nick end labeling assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007464 tryptic phosphopeptide mapping assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007433 mass spectrometry evidence used in automatic assertion A type of tryptic phosphopeptide mapping assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007465 transgenic organism evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007427 genetic transformation evidence used in automatic assertion A type of transgenic organism evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007466 tissue microarray evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007375 cytochemistry evidence used in automatic assertion A type of tissue microarray evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007467 TACE activity assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007468 enzymatic activity assay evidence used in automatic assertion A type of TACE activity assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007468 enzymatic activity assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of enzyme assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007469 surface plasmon resonance evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007332 physical interaction evidence used in automatic assertion A type of surface plasmon resonance evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007470 restriction landmark genomic scanning evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000089 restriction landmark genomic scanning evidence A type of restriction landmark genomic scanning evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007471 resonant mirror biosensor evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of resonant mirror biosensor evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007472 high-performance liquid chromatography evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007681 chromatography evidence used in automatic assertion A type of high-performance liquid chromatography evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007473 ectopic expression evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000017 ectopic expression evidence A type of ectopic expression evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007474 electrophoretic mobility shift assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007332 physical interaction evidence used in automatic assertion A type of electrophoretic mobility shift assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007475 reverse transcription polymerase chain reaction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000109 reverse transcription polymerase chain reaction evidence A type of reverse transcription polymerase chain reaction evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007476 in vivo polyADP-ribosylation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005500 in vivo polyADP-ribosylation assay evidence A type of in vivo polyADP-ribosylation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007477 DNA dot blot assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005522 DNA dot blot assay evidence A type of DNA dot blot assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007478 random mutagenesis evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of random mutagenesis phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007479 biological system reconstruction evidence by experimental evidence from single species used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005553 biological system reconstruction evidence by experimental evidence from single species A type of biological system reconstruction evidence by experimental evidence from single species that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007480 biological system reconstruction evidence by experimental evidence from mixed species used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005552 biological system reconstruction evidence by experimental evidence from mixed species A type of biological system reconstruction evidence by experimental evidence from mixed species that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007481 biological system reconstruction evidence based on orthology evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007482 biological system reconstruction evidence based on homology evidence used in automatic assertion A type of biological system reconstruction evidence based on orthology evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007482 biological system reconstruction evidence based on homology evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005549 biological system reconstruction evidence based on homology evidence A type of biological system reconstruction evidence based on homology evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007483 biological system reconstruction evidence based on paralogy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007482 biological system reconstruction evidence based on homology evidence used in automatic assertion A type of biological system reconstruction evidence based on paralogy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007484 biological system reconstruction evidence based on inference from background scientific knowledge used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005548 biological system reconstruction evidence based on inference from background scientific knowledge A type of biological system reconstruction evidence based on inference from background scientific knowledge that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007485 immunogold labelling evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007486 immunolocalization evidence used in automatic assertion A type of immunogold labelling evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007486 immunolocalization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007435 microscopy evidence used in automatic assertion A type of immunolocalization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007487 flow cytometry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of flow cytometry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007488 enzyme-linked immunoabsorbent assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000267 enzyme-linked immunoabsorbent assay evidence A type of enzyme-linked immunoabsorbent assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007489 high throughput mass spectrometry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007433 mass spectrometry evidence used in automatic assertion A type of high throughput mass spectrometry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007490 confocal microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007435 microscopy evidence used in automatic assertion A type of confocal microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007491 wide-field microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007435 microscopy evidence used in automatic assertion A type of wide-field microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007492 immunogold labelling electron microscopy assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007493 electron microscopy evidence used in automatic assertion A type of immunogold labelling electron microscopy assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007493 electron microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007435 microscopy evidence used in automatic assertion A type of electron microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007494 immunoperoxidase immunolocalization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007486 immunolocalization evidence used in automatic assertion A type of immunoperoxidase immunolocalization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007495 immunoperoxidase immunolocalization electron microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007494 immunoperoxidase immunolocalization evidence used in automatic assertion A type of immunoperoxidase immunolocalization electron microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007496 immunofluorescence confocal microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007497 immunofluorescence evidence used in automatic assertion A type of immunofluorescence confocal microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007497 immunofluorescence evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000007 immunofluorescence evidence A type of immunofluorescence evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007498 two-dimensional polyacrylamide gel electrophoresis evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005503 two-dimensional polyacrylamide gel electrophoresis evidence A type of two-dimensional polyacrylamide gel electrophoresis evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007499 alkaline phosphatase reporter gene assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001801 alkaline phosphatase reporter gene assay evidence A type of alkaline phosphatase reporter gene assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007500 beta-galactosidase reporter gene assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001802 beta-galactosidase reporter gene assay evidence A type of beta-galactosidase reporter gene assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007501 chloramphenicol acetyltransferase reporter gene assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001803 chloramphenicol acetyltransferase reporter gene assay evidence A type of chloramphenicol acetyltransferase reporter gene assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007502 chromatin immunoprecipitation-PCR evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000227 chromatin immunoprecipitation-PCR evidence A type of chromatin immunoprecipitation-PCR evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007503 immunoprecipitation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007373 protein binding evidence used in automatic assertion A type of immunoprecipitation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007504 copper-phenanthroline footprinting evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007505 nucleic acid binding evidence used in automatic assertion A type of copper-phenanthroline footprinting evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007505 nucleic acid binding evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007384 affinity evidence used in automatic assertion A type of nucleic acid binding evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007506 DNA affinity chromatography evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007681 chromatography evidence used in automatic assertion A type of DNA affinity chromatography evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007507 DNAse footprinting evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007505 nucleic acid binding evidence used in automatic assertion A type of DNAse footprinting evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007508 fluorescence anisotropy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007450 fluorescence evidence used in automatic assertion A type of fluorescence anisotropy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007509 ferric uptake regulator titration assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001829 ferric uptake regulator titration assay evidence A type of ferric uptake regulator titration assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007510 systematic evolution of ligands by exponential amplification evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007332 physical interaction evidence used in automatic assertion A type of systematic evolution of ligands by exponential amplification evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007511 glutathione S-transferase pull-down assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007432 bait-prey hybrid interaction evidence used in automatic assertion A type of glutathione S-transferase pull-down assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007512 beta-glucuronidase reporter gene assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001804 beta-glucuronidase reporter gene assay evidence A type of beta-glucuronidase reporter gene assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007513 heteronuclear single quantum coherence spectroscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006164 nuclear magnetic resonance spectroscopy evidence used in automatic assertion A type of heteronuclear single quantum coherence spectroscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007514 hydroxyl-radical footprinting evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007505 nucleic acid binding evidence used in automatic assertion A type of hydroxyl-radical footprinting evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007515 isothermal titration calorimetry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007384 affinity evidence used in automatic assertion A type of isothermal titration calorimetry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007516 luciferase reporter gene assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001805 luciferase reporter gene assay evidence A type of luciferase reporter gene assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007517 methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007505 nucleic acid binding evidence used in automatic assertion A type of methidiumpropyl-ethylenediaminetetraacetic acid iron (II) footprinting evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007518 northern blot evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007303 transcript expression evidence used in automatic assertion A type of northern blot evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007519 methylation interference footprinting evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007505 nucleic acid binding evidence used in automatic assertion A type of methylation interference footprinting evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007520 primer extension assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007303 transcript expression evidence used in automatic assertion A type of primer extension assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007521 quantitative polymerase chain reaction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000231 quantitative polymerase chain reaction evidence A type of quantitative polymerase chain reaction evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007522 rapid amplification of cDNA ends polymerase chain reaction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001820 rapid amplification of cDNA ends polymerase chain reaction evidence A type of rapid amplification of cDNA ends polymerase chain reaction evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007523 S1 nuclease protection assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007316 nuclease protection assay evidence used in automatic assertion A type of S1 nuclease protection assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007524 site-directed mutagenesis phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of site-directed mutagenesis phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007525 survival rate analysis evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007370 knockout evidence used in automatic assertion A type of survival rate analysis evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007526 ultraviolet light footprinting evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007505 nucleic acid binding evidence used in automatic assertion A type of ultraviolet light footprinting evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007527 xylE reporter gene assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005537 xylE reporter gene assay evidence A type of xylE reporter gene assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007528 ad-hoc qualitative phenotype observation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005673 ad-hoc qualitative phenotype observation evidence A type of ad-hoc qualitative phenotype observation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007529 ad-hoc quantitative phenotype observation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005675 ad-hoc quantitative phenotype observation evidence A type of ad-hoc quantitative phenotype observation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007530 cell transfection experiment evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of cell transfection experiment evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007531 yeast 2-hybrid evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007432 bait-prey hybrid interaction evidence used in automatic assertion A type of yeast 2-hybrid evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007532 Cya fusion reporter assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007473 ectopic expression evidence used in automatic assertion A type of Cya fusion reporter assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007533 super-resolution microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007435 microscopy evidence used in automatic assertion A type of super-resolution microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007534 fractionation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of fractionation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007535 electrophysiology assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of electrophysiology assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007536 mRNA interactome capture evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007505 nucleic acid binding evidence used in automatic assertion A type of mRNA interactome capture evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007537 patch-clamp recording evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007535 electrophysiology assay evidence used in automatic assertion A type of patch-clamp recording evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007538 whole-cell patch-clamp recording evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007537 patch-clamp recording evidence used in automatic assertion A type of whole-cell patch-clamp recording evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007539 author statement supported from referenced clinical study used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007321 author statement supported by traceable reference used in automatic assertion A type of author statement supported from referenced clinical study that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007540 inference based on individual clinical experience used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007322 curator inference used in automatic assertion A type of inference based on individual clinical experience that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007541 biofilm formation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006020 biofilm formation assay evidence A type of biofilm formation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007542 microtiter plate biofilm assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007541 biofilm formation assay evidence used in automatic assertion A type of microtiter plate biofilm assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007543 air-liquid interface assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007541 biofilm formation assay evidence used in automatic assertion A type of air-liquid interface assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007544 colony biofilm assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007541 biofilm formation assay evidence used in automatic assertion A type of colony biofilm assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007545 Kadouri drip-fed biofilm assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007541 biofilm formation assay evidence used in automatic assertion A type of Kadouri drip-fed biofilm assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007546 co-immunoprecipitation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007503 immunoprecipitation evidence used in automatic assertion A type of co-immunoprecipitation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007547 optogenetic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006032 optogenetic evidence A type of optogenetic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007548 fluorescent sensor evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007450 fluorescence evidence used in automatic assertion A type of fluorescent sensor evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007549 genetically encoded fluorescent sensor evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007548 fluorescent sensor evidence used in automatic assertion A type of genetically encoded fluorescent sensor evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007550 genetically encoded fluorescent electrophysiology assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007548 fluorescent sensor evidence used in automatic assertion A type of genetically encoded fluorescent electrophysiology assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007551 genetically encoded fluorescent ion concentration sensor assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007550 genetically encoded fluorescent electrophysiology assay evidence used in automatic assertion A type of genetically encoded fluorescent ion concentration sensor assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007552 cell fractionation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007534 fractionation evidence used in automatic assertion A type of cell fractionation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007553 extracellular recording evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007535 electrophysiology assay evidence used in automatic assertion A type of extracellular recording evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007554 single-unit extracellular recording evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007553 extracellular recording evidence used in automatic assertion A type of single-unit extracellular recording evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007555 field potential recording evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007553 extracellular recording evidence used in automatic assertion A type of field potential recording evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007556 anti-sense experiment evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of anti-sense experiment evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007557 morpholino experiment evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of morpholino experiment evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007558 RNAi evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of RNAi evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007559 pharmacological assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007635 experimental phenotypic evidence used in automatic assertion A type of pharmacological assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007560 immunofluorescence wide-field microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007561 wide-field fluorescence microscopy evidence used in automatic assertion A type of immunofluorescence wide-field microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007561 wide-field fluorescence microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007491 wide-field microscopy evidence used in automatic assertion A type of wide-field fluorescence microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007562 over expression analysis evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007427 genetic transformation evidence used in automatic assertion A type of over expression analysis evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007563 cell-free assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of cell-free assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007564 fluorescence recovery after photobleaching evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007450 fluorescence evidence used in automatic assertion A type of fluorescence recovery after photobleaching evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007565 immuno-labelling electron microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007493 electron microscopy evidence used in automatic assertion A type of immuno-labelling electron microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007566 immunofluorescence super resolution microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007533 super-resolution microscopy evidence used in automatic assertion A type of immunofluorescence super resolution microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007567 co-purification evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007332 physical interaction evidence used in automatic assertion A type of co-purification evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007568 yeast one-hybrid evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007432 bait-prey hybrid interaction evidence used in automatic assertion A type of yeast one-hybrid evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007569 split-ubiquitin functional complementation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000074 split-ubiquitin functional complementation evidence A type of split-ubiquitin functional complementation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007570 far-Western blotting evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007332 physical interaction evidence used in automatic assertion A type of far-Western blotting evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007571 affinity chromatography evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007384 affinity evidence used in automatic assertion A type of affinity chromatography evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007572 ribohomopolymer binding assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007505 nucleic acid binding evidence used in automatic assertion A type of ribohomopolymer binding assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007573 protein:ion binding evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007373 protein binding evidence used in automatic assertion A type of protein:ion binding evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007574 Southwestern blot evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007505 nucleic acid binding evidence used in automatic assertion A type of Southwestern blot evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007575 Northwestern blot evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007505 nucleic acid binding evidence used in automatic assertion A type of Northwestern blot evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007576 bacterial one-hybrid evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007432 bait-prey hybrid interaction evidence used in automatic assertion A type of bacterial one-hybrid evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007577 protein-oligonucleotide microarray binding evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007505 nucleic acid binding evidence used in automatic assertion A type of protein-oligonucleotide microarray binding evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007578 functional complementation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007326 genetic interaction evidence used in automatic assertion A type of functional complementation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007579 transgenic rescue experiment evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007578 functional complementation evidence used in automatic assertion A type of transgenic rescue experiment evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007580 transient rescue experiment evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007578 functional complementation evidence used in automatic assertion A type of transient rescue experiment evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007581 suppressor/enhancer interaction phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007326 genetic interaction evidence used in automatic assertion A type of suppressor/enhancer interaction phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007582 double mutant phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of double mutant phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007583 epistatic interaction phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007326 genetic interaction evidence used in automatic assertion A type of epistatic interaction phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007584 functional complementation in heterologous system evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007578 functional complementation evidence used in automatic assertion A type of functional complementation in heterologous system evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007585 temperature-sensitive mutant phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of temperature-sensitive mutant phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007586 recessive mutant phenotype evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of recessive mutant phenotype evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007587 high throughput mutant phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007325 mutant phenotype evidence used in automatic assertion A type of high throughput mutant phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007588 high throughput genetic interaction phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007326 genetic interaction evidence used in automatic assertion A type of high throughput genetic interaction phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007589 high throughput direct assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007307 direct assay evidence used in automatic assertion A type of high throughput direct assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007590 high throughput expression pattern evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007298 expression pattern evidence used in automatic assertion A type of high throughput expression pattern evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007591 radioligand binding assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007008 radioligand binding assay evidence A type of radioligand binding assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007592 combinatorial experimental and author inference evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000213 combinatorial evidence used in automatic assertion A type of combinatorial evidence from author knowledge and experimental evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007593 combinatorial experimental and curator inference evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000213 combinatorial evidence used in automatic assertion A type of combinatorial evidence from curator knowledge and experimental evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007594 voltammetry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007015 voltammetry evidence A type of voltammetry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007595 photoconversion evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007450 fluorescence evidence used in automatic assertion A type of photoconversion evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007596 agglutination test evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007019 agglutination test evidence A type of agglutination test evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007597 slide agglutination test evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007021 slide agglutination test evidence A type of slide agglutination test evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007598 direct Coombs test evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007023 direct Coombs test evidence A type of direct Coombs test evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007599 indirect Coombs test evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007025 indirect Coombs test evidence A type of indirect Coombs test evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007600 direct hemagglutination assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007027 direct hemagglutination assay evidence A type of direct hemagglutination assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007601 viral hemagglutination inhibition assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007029 viral hemagglutination inhibition assay evidence A type of viral hemagglutination inhibition assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007602 compement fixation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007031 compement fixation assay evidence A type of compement fixation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007603 neutralization test assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007033 neutralization test assay evidence A type of neutralization test assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007604 copper transport assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007035 copper transport assay evidence A type of copper transport assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007605 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007037 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence A type of 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007606 plaque assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007039 plaque assay evidence A type of plaque assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007607 epifluorescence microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007041 epifluorescence microscopy evidence A type of epifluorescence microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007608 transmission electron microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007043 transmission electron microscopy evidence A type of transmission electron microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007609 scanning electron microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007045 scanning electron microscopy evidence A type of scanning electron microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007610 time-lapsed microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007047 time-lapsed microscopy evidence A type of time-lapsed microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007611 phase contrast microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007049 phase contrast microscopy evidence A type of phase contrast microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007612 transmitted light brightfied mircoscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007051 transmitted light brightfied mircoscopy evidence A type of transmitted light brightfied mircoscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007613 koehler illumination microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007053 koehler illumination microscopy evidence A type of koehler illumination microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007614 differential interference contrast microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007055 differential interference contrast microscopy evidence A type of differential interference contrast microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007615 extended field laser confocal microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007057 extended field laser confocal microscopy evidence A type of extended field laser confocal microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007616 confocal laser scanning microscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007059 confocal laser scanning microscopy evidence A type of confocal laser scanning microscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007617 light scattering evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007061 light scattering evidence A type of light scattering evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007618 dynamic light scattering assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007063 dynamic light scattering assay evidence A type of dynamic light scattering assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007619 static light scattering assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007065 static light scattering assay evidence A type of static light scattering assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007620 colony papillation assay phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007067 colony papillation assay phenotypic evidence A type of colony papillation assay phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007621 crystal violet staining evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007069 crystal violet staining evidence A type of crystal violet staining evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007622 flow cell biofilm assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007071 flow cell biofilm assay evidence A type of flow cell biofilm assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007623 bacterial 2-hybrid assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007073 bacterial 2-hybrid assay evidence A type of bacterial 2-hybrid assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007624 phenomic profiling assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007075 phenomic profiling assay evidence A type of phenomic profiling assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007625 colony morphology phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007077 colony morphology phenotypic evidence A type of colony morphology phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007626 colony color phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007079 colony color phenotypic evidence A type of colony color phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007627 colony size phenotypic evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007081 colony size phenotypic evidence A type of colony size phenotypic evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007628 zone of inhibition evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007083 zone of inhibition evidence A type of zone of inhibition evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007629 Etest evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007085 Etest evidence A type of Etest evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007630 ribosome profiling evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007298 expression pattern evidence used in automatic assertion A type of ribosome profiling evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007631 computational inference used in manual assertion http://purl.obolibrary.org/obo/ECO_0000362 computational inference A type of evidence based on computational logical inference that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007632 transcriptional activation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000314 direct assay evidence used in manual assertion A type of transcriptional activation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007633 transcriptional activation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0000170 transcriptional activation assay evidence A type of transcriptional activation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/OBI_0002590 molecular interaction identification design http://purl.obolibrary.org/obo/OBI_0500000 study design A study design that investigates the features of interaction between molecules. http://purl.obolibrary.org/obo/OBI_0002619 site-directed mutagenesis http://purl.obolibrary.org/obo/OBI_0001154 induced mutagenesis An induced mutation in which a specific base change is programmed into the sequence of a synthetic primer. http://purl.obolibrary.org/obo/OBI_0002620 random mutagenesis http://purl.obolibrary.org/obo/OBI_0001154 induced mutagenesis An induced mutation that produces a random mutation, either by introducing the organism to mutagens, or through a process such as error-prone PCR. http://purl.obolibrary.org/obo/OBI_0002621 reconstitution assay http://purl.obolibrary.org/obo/OBI_0000471 study design execution An assay in which a cellular process is replicated from the bottom-up using isolated components in order to observe how the components work together. http://purl.obolibrary.org/obo/OBI_0002622 in vitro transcription reconstitution assay http://purl.obolibrary.org/obo/OBI_0002621 reconstitution assay A reconstitution assay in which RNA synthesis is investigated using RNA polymerase II transcription systems replicated in vitro from their base components. http://purl.obolibrary.org/obo/OBI_0002625 gene knockdown http://purl.obolibrary.org/obo/OBI_0600043 genetic transformation A genetic transformation which reduces the expression of a specific gene or genes in an organism. http://purl.obolibrary.org/obo/ECO_0006163 nuclear magnetic resonance spectroscopy evidence http://purl.obolibrary.org/obo/ECO_0001105 nuclear magnetic resonance evidence A type of nuclear magnetic resonance evidence used for quantification of metabolites or for the determination of chemical structure or composition. http://purl.obolibrary.org/obo/ECO_0006164 nuclear magnetic resonance spectroscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006163 nuclear magnetic resonance spectroscopy evidence A type of nuclear magnetic resonance spectroscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006165 nuclear magnetic resonance spectroscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006163 nuclear magnetic resonance spectroscopy evidence A type of nuclear magnetic resonance spectroscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006166 nuclear magnetic resonance imaging evidence http://purl.obolibrary.org/obo/ECO_0001105 nuclear magnetic resonance evidence A type of nuclear magnetic resonance evidence used to image anatomy and physiological processes. http://purl.obolibrary.org/obo/ECO_0006167 nuclear magnetic resonance imaging evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006166 nuclear magnetic resonance imaging evidence A type of nuclear magnetic resonance imaging evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006168 nuclear magnetic resonance imaging evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006166 nuclear magnetic resonance imaging evidence A type of nuclear magnetic resonance imaging evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006169 quantitative western immunoblotting evidence http://purl.obolibrary.org/obo/ECO_0000112 qualitative western immunoblotting evidence A type of qualitative western immunoblotting evidence where detection of the signal is carried out through digital imaging and the signal is normalized with methods such as standard curves built from reference housekeeping proteins or total transferred protein measurements. http://purl.obolibrary.org/obo/ECO_0006170 quantitative western immunoblotting evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006169 quantitative western immunoblotting evidence A type of quantitative western immunoblotting evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006171 quantitative western immunoblotting evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006169 quantitative western immunoblotting evidence A type of quantitatative western immunoblotting evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/OBI_0003043 urine assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that has a urine specimen as evaluant. http://purl.obolibrary.org/obo/ECO_0008009 single molecule fluorescence in situ hybridization evidence http://purl.obolibrary.org/obo/ECO_0005518 RNA detection assay evidence A type of fluorescence in situ hybridization evidence resulting from the use of fluorescent probes to localize and quantify individual RNA molecules in single cells for studying gene expression. http://purl.obolibrary.org/obo/ECO_0008010 support vector machine evidence http://purl.obolibrary.org/obo/ECO_0008000 machine learning method evidence A type of machine learning method evidence that uses a supervised learning model with associated algorithms for classification. Support vector machines aim to determine a hyperplane that separates two classes while maximizing the distance from the hyperplane to any given data point in those classes. http://purl.obolibrary.org/obo/ECO_0008011 string-matching method evidence http://purl.obolibrary.org/obo/ECO_0007672 computational evidence A type of computational evidence that employs a string algorithm to identify a string within a larger string or body of text, where a string is a particular series of characters consisting of letters, numbers, symbols, spaces, etc. http://purl.obolibrary.org/obo/ECO_0008012 regular expression method evidence http://purl.obolibrary.org/obo/ECO_0008011 string-matching method evidence A type of string-matching method evidence in which patterns consisting of characters and/or symbols in particular combinations and/or order are used to identify matches in a larger string or body of text. http://purl.obolibrary.org/obo/ECO_0008013 neural network method evidence http://purl.obolibrary.org/obo/ECO_0008000 machine learning method evidence A type of machine learning method evidence in which the machine learning model consists of nodes connected to each other with edges that are organized into layers, where the edges can transmit signals between nodes such that the system acts similarly to biological neurons. http://purl.obolibrary.org/obo/ECO_0008014 coordinate mapping to geographic property shapefiles http://purl.obolibrary.org/obo/ECO_0000362 computational inference A type of computational inference where a geographic property of an entity is determined by searching coordinates associated with the entity against polygons with associated geographic properties in the form of shapefiles. http://purl.obolibrary.org/obo/ECO_0008015 inference of intermediate molecular structure from structure of final molecule http://purl.obolibrary.org/obo/ECO_0000205 curator inference A type of curator inference in which the structure of a possible intermediate molecule made during a biosynthetic process is inferred based on the structure of the final molecule in the process. http://purl.obolibrary.org/obo/ECO_0008016 single molecule fluorescence in situ hybridization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008009 single molecule fluorescence in situ hybridization evidence A type of single molecule fluorescence in situ hybridization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008017 single molecule fluorescence in situ hybridization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008009 single molecule fluorescence in situ hybridization evidence A type of single molecule fluorescence in situ hybridization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008018 support vector machine evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008010 support vector machine evidence A type of support vector machine evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008019 support vector machine evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008010 support vector machine evidence A type of support vector machine evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008020 string-matching method evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008011 string-matching method evidence A type of string-matching method evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008021 string-matching method evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008011 string-matching method evidence A type of string-matching method evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008022 regular expression method evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008020 string-matching method evidence used in manual assertion A type of regular expression method evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008023 regular expression method evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008021 string-matching method evidence used in automatic assertion A type of regular expression method evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008024 neural network method evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0008013 neural network method evidence A type of neural network method evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008025 neural network method evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008013 neural network method evidence A type of neural network method evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008026 coordinate mapping to geographic property shapefiles used in manual assertion http://purl.obolibrary.org/obo/ECO_0008014 coordinate mapping to geographic property shapefiles A type of coordinate mapping to geographic property shapefiles that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008027 coordinate mapping to geographic property shapefiles used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008014 coordinate mapping to geographic property shapefiles A type of coordinate mapping to geographic property shapefiles that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0008028 inference of intermediate molecular structure from structure of final molecule used in manual assertion http://purl.obolibrary.org/obo/ECO_0008015 inference of intermediate molecular structure from structure of final molecule A type of inference of intermediate molecular structure from structure of final molecule that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0008029 inference of intermediate molecular structure from structure of final molecule used in automatic assertion http://purl.obolibrary.org/obo/ECO_0008015 inference of intermediate molecular structure from structure of final molecule A type of inference of intermediate molecular structure from structure of final molecule that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0001845 cell population optical density evidence http://purl.obolibrary.org/obo/ECO_0001565 cell-based assay evidence A type of cell-based assay evidence in which optical density is used to measure some aspect of a population of cells. Examples of aspects that can affect optical density measurements include, but are not limited to, cell number and cell shape. http://purl.obolibrary.org/obo/ECO_0001846 cell population optical density evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001845 cell population optical density evidence A type of cell population optical density evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0001847 cell population optical density evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001845 cell population optical density evidence A type of cell population optical density evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/CHMO_0000087 fluorescence microscopy assay http://purl.obolibrary.org/obo/CHMO_0000102 light microscopy assay Any type of light microscopy assay where the specimen can be made to fluoresce (emit energy as visible light) by illuminating it with light of specific wavelengths. These specimens are called fluorophores. http://purl.obolibrary.org/obo/CHMO_0000102 light microscopy assay http://purl.obolibrary.org/obo/OBI_0002119 microscopy assay Microscopy where the specimen is illuminated with visible light and a system of lenses is used to produce an image. http://purl.obolibrary.org/obo/ECO_0007008 radioligand binding assay evidence http://purl.obolibrary.org/obo/ECO_0000024 protein binding evidence A type of protein binding evidence in which radioactive ligands are used to measure receptor-ligand interactions, such as in determining selectivity for a particular ligand. http://purl.obolibrary.org/obo/ECO_0007009 radioligand binding assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007008 radioligand binding assay evidence A type of radioligand binding assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001522 small molecule detection assay evidence http://purl.obolibrary.org/obo/ECO_0005516 molecule detection assay evidence A type of molecule detection assay evidence resulting from the detection and quantification of a small molecule (a low molecular weight (typically < 900 daltons) organic compound), such as lipids, sugars, animo acids, drugs, etc. http://purl.obolibrary.org/obo/ECO_0007636 curator inference from database http://purl.obolibrary.org/obo/ECO_0007647 curator inference from authoritative source A type of curator inference from authoritative resource based on information located in a queryable database and is optimized for computers. http://purl.obolibrary.org/obo/ECO_0007637 curator inference from encyclopedia http://purl.obolibrary.org/obo/ECO_0007647 curator inference from authoritative source A type of curator inference from published work where the reference is to an entry in a compendium that provides summarized information on a subject. http://purl.obolibrary.org/obo/ECO_0007638 curator inference from Wikipedia http://purl.obolibrary.org/obo/ECO_0007637 curator inference from encyclopedia A type of curator inference from encyclopedia where the reference is to a Wikipedia article. http://purl.obolibrary.org/obo/ECO_0007639 curator inference from Britannica http://purl.obolibrary.org/obo/ECO_0007637 curator inference from encyclopedia A type of curator inference from encyclopedia where the reference is to an Encyclopedia Britannica article. http://purl.obolibrary.org/obo/ECO_0007640 curator inference from MedlinePlus encyclopedia http://purl.obolibrary.org/obo/ECO_0007637 curator inference from encyclopedia A type of curator inference from encyclopedia in which the reference is to an article in the National Library of Medicine's MedLinePlus encyclopedia. http://purl.obolibrary.org/obo/ECO_0007641 curator inference from dictionary http://purl.obolibrary.org/obo/ECO_0007647 curator inference from authoritative source A type of curator inference from published work in which the entry comes from a collection of words with definitions, usages, pronounciations, and more. http://purl.obolibrary.org/obo/ECO_0007642 curator inference from Oxford Dictionary http://purl.obolibrary.org/obo/ECO_0007641 curator inference from dictionary A type of curator inference from dictionary in which the reference is to an entry in the Oxford Dictionaries. http://purl.obolibrary.org/obo/ECO_0007643 curator inference from Merriam-Webster Dictionary http://purl.obolibrary.org/obo/ECO_0007641 curator inference from dictionary A type of curator inference from dictionary in which the reference is to an entry in the Merriam-Webster Dictionary. http://purl.obolibrary.org/obo/ECO_0007644 curator inference from MedlinePlus dictionary http://purl.obolibrary.org/obo/ECO_0007641 curator inference from dictionary A type of curator inference from dictionary in which the reference is to an entry in the National Library of Medicine's MedLinePlus dictionary. http://purl.obolibrary.org/obo/ECO_0007645 curator inference from journal publication http://purl.obolibrary.org/obo/ECO_0007647 curator inference from authoritative source A type of curator inference from published work reporting on research findings. http://purl.obolibrary.org/obo/ECO_0007646 curator inference from book http://purl.obolibrary.org/obo/ECO_0007647 curator inference from authoritative source A type of curator inference from published work based on a book, which may be a reference to a URL (for ebooks) or a DOI. http://purl.obolibrary.org/obo/ECO_0007647 curator inference from authoritative source http://purl.obolibrary.org/obo/ECO_0000205 curator inference A type of curator inference that is from what is generally considered an authoritative source on the topic, including model organism databases, newspaper articles, books, journal publications, etc. http://purl.obolibrary.org/obo/ECO_0007680 chromatography evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000325 chromatography evidence A type of chromatography evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007681 chromatography evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007297 experimental evidence used in automatic assertion A type of chromatography evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/OBI_0003117 3D molecular structure determination assay http://purl.obolibrary.org/obo/OBI_0600045 3D structure determination assay An assay that determines the 3-dimensional configuration of a molecular entity or molecular complex. http://purl.obolibrary.org/obo/OBI_0003119 antigen:antibody complex http://purl.obolibrary.org/obo/COB_0000080 complex of molecular entities A complex of molecular entitites which is the result of an antibody binding to an antigen. http://purl.obolibrary.org/obo/OBI_0003120 epitope:MHC:TCR complex http://purl.obolibrary.org/obo/COB_0000080 complex of molecular entities A complex of molecular entities which is the result of a T cell receptor binding to a MHC-epitope complex. http://purl.obolibrary.org/obo/OBI_0003121 MHC:ligand complex http://purl.obolibrary.org/obo/COB_0000080 complex of molecular entities A complex of molecular entities which is the result of a MHC molecule binding with a ligand. http://purl.obolibrary.org/obo/OBI_0003122 3D cell structure determination assay http://purl.obolibrary.org/obo/OBI_0600045 3D structure determination assay A 3D structure determination assay that determines the 3-dimensional configuration of a cell. http://purl.obolibrary.org/obo/OBI_0003123 3D neural cell structure determination assay http://purl.obolibrary.org/obo/OBI_0003122 3D cell structure determination assay A 3D cell structure determination assay that determines the 3-dimensional configuration of a neural cell. http://purl.obolibrary.org/obo/OBI_0003128 T cell epitope assay http://purl.obolibrary.org/obo/OBI_1110128 immune epitope assay An immune epitope assay that characterizes the structrue of an epitope-T cell receptor complex, or measures the binding of a T cell receptor to epitope, or the immune response process resulting from such a binding event or the recognition of the epitope. http://purl.obolibrary.org/obo/OBI_0003142 quantitative binding datum http://purl.obolibrary.org/obo/OBI_0001181 binding datum A binding datum that is expressed using numbers. http://purl.obolibrary.org/obo/OBI_0003147 assay measuring quantitative binding of an epitope:MHC:TCR complex http://purl.obolibrary.org/obo/OBI_1110037 assay measuring binding of a T cell epitope:MHC:TCR complex An assay that detects the binding of a MHC:epitope complex with a T cell receptor, and produces a quantitative measurement of the binding as an output. http://purl.obolibrary.org/obo/OBI_0002436 RNA interactome capture http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that identifies RNA binding proteins by cross-linking RNA and proteins with UV light, then purifying the bound complexes by oligo(dT) capture. Finally, the complexes are analyzed by mass spectrometry. http://purl.obolibrary.org/obo/OBI_0002438 nuclear ligation assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that detects the proximity of chromosomal DNA through the use of a ligation reaction in isolated nuclei. http://purl.obolibrary.org/obo/OBI_0002439 chromosome conformation capture assay http://purl.obolibrary.org/obo/OBI_0002438 nuclear ligation assay A nuclear ligation assay that detects chromosomal interactions between any two genomic loci. Chromatin segments are cross-linked, cut by restriction enzymes, ligated, and finally analyzed by PCR. http://purl.obolibrary.org/obo/OBI_0002440 Hi-C assay http://purl.obolibrary.org/obo/OBI_0002439 chromosome conformation capture assay A chromosome conformation capture assay that detects genome-wide chromosomal interactions. High-throughput techniques are used to sequence the ligated fragments after cross-linking and cutting with restriction enzymes. http://purl.obolibrary.org/obo/OBI_0002692 polymerase chain reaction assay http://purl.obolibrary.org/obo/OBI_0000070 assay An assay that uses polymerase chain reaction technique to amplify DNA. http://purl.obolibrary.org/obo/OBI_0002743 molecular label role http://purl.obolibrary.org/obo/OBI_0000086 reagent role A reagent role inhering in a molecular entity intended to associate with some molecular target to serve as a proxy for the presence, abundance, or location of this target in a detection of molecular label assay. http://purl.obolibrary.org/obo/OBI_0002744 molecular label http://purl.obolibrary.org/obo/OBI_0001879 reagent A molecular reagent intended to associate with some molecular target to serve as a proxy for the presence, abundance, or location of this target in a detection of molecular label assay http://purl.obolibrary.org/obo/OBI_0003024 lymphocyte assay http://purl.obolibrary.org/obo/OBI_0001977 cytometry assay A cytometry assay that measures the abundance and/or characteristics of lymphocytes http://purl.obolibrary.org/obo/OBI_2100023 protein assay http://purl.obolibrary.org/obo/OBI_0000443 analyte assay An analyte assay that measures the abundance of protein http://purl.obolibrary.org/obo/ECO_0001836 in situ hybridization evidence http://purl.obolibrary.org/obo/ECO_0000008 expression pattern evidence A type of expression pattern evidence in which single-stranded DNA or RNA (probe) are annealed to complementary DNA or RNA in a portion or section of tissue. http://purl.obolibrary.org/obo/ECO_0001837 in situ hybridization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001836 in situ hybridization evidence A type of in situ hybridization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0001838 in situ hybridization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001836 in situ hybridization evidence A type of in situ hybridization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001839 colorimetric in situ hybridization evidence http://purl.obolibrary.org/obo/ECO_0001836 in situ hybridization evidence A type of nucleic acid localization evidence and in situ hybridization evidence resulting from the use of colorimetric probes to detect complementary sequences of nucleic acids. http://purl.obolibrary.org/obo/ECO_0001840 colorimetric in situ hybridization evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0001839 colorimetric in situ hybridization evidence A type of colorimetric in situ hybridization evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0001841 colorimetric in situ hybridization evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001839 colorimetric in situ hybridization evidence A type of colorimetric in situ hybridization evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/OBI_0003127 B cell epitope assay http://purl.obolibrary.org/obo/OBI_1110128 immune epitope assay An immune epitope assay that characterizes the structrue of an antibody-epitope complex, or measures the binding of an antibody receptor to epitope, or the immune response process resulting from such a binding event or the recognition of the epitope. http://purl.obolibrary.org/obo/ECO_0006263 turbidity measurement evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where the optical properties of a solution is measured in the visible light spectrum, assessing the haziness or cloudiness as a result of the properties of the solutes or the changes in their material states. http://purl.obolibrary.org/obo/ECO_0006331 Congo red staining evidence http://purl.obolibrary.org/obo/ECO_0005019 staining evidence A type of staining evidence where Congo red is used to stain cellular structures including amyloid fibrils and aggregates, cell walls of plants and fungi, and for the outer membrane of Gram-negative bacteria. http://purl.obolibrary.org/obo/ECO_0006332 Congo red birefringence evidence http://purl.obolibrary.org/obo/ECO_0006331 Congo red staining evidence A type of Congo red staining evidence where the Congo red dye produces an apple-green birefringence when it specifically binds to cross-β-sheets of amyloids which is detectable via cross-polarized light. http://purl.obolibrary.org/obo/ECO_0006333 thioflavin T based fluorescence spectroscopy evidence http://purl.obolibrary.org/obo/ECO_0001115 fluorescence evidence A type of fluorescence evidence used to assess the formation of amyloid fibrils indicated by an increase in Thioflavin T fluorescence intensity and a characteristic red shift in the emission spectrum upon binding to amyloids. http://purl.obolibrary.org/obo/ECO_0006334 differential scanning fluorimetry evidence http://purl.obolibrary.org/obo/ECO_0001115 fluorescence evidence A type of protein thermal shift assay evidence where thermal unfolding is monitored by measuring the fluorescence intensity upon interaction with its environment. http://purl.obolibrary.org/obo/ECO_0006335 fluorescence correlation spectroscopy evidence http://purl.obolibrary.org/obo/ECO_0001115 fluorescence evidence A type of fluorescence evidence that measures fluctuations in fluorescence intensity used to detect biophysical properties of single molecules such as diffusion coefficients and hydrodynamic radii. http://purl.obolibrary.org/obo/ECO_0006336 intrinsic fluorescence-based protein conformation evidence http://purl.obolibrary.org/obo/ECO_0001115 fluorescence evidence A type of fluorescence evidence used to study protein conformation by measuring the emissions of aromatic amino acids, specifically tryptophan, tyrosine and, to a minor extent, phenylalanine residues. http://purl.obolibrary.org/obo/ECO_0006337 stopped-flow fluorescence spectroscopy evidence http://purl.obolibrary.org/obo/ECO_0001115 fluorescence evidence A type of fluorescence evidence used to study binding kinetics by rapidly mixing two interaction components and then "stopping" the reaction in the observation chamber. http://purl.obolibrary.org/obo/ECO_0006338 temperature jump relaxation evidence http://purl.obolibrary.org/obo/ECO_0001115 fluorescence evidence A type of fluorescence evidence where the rates of fast chemical reactions in a solution are measured by the intensity of light absorbed or fluoresced by allowing the reaction mixture to come to an equilibrium, and then perturbing the equilibrium by a rapid temperature rise followed by relaxation of the system to the new equilibrium. http://purl.obolibrary.org/obo/ECO_0006339 structural ensemble generation evidence http://purl.obolibrary.org/obo/ECO_0006341 computational structure modeling evidence A type of computational structure modeling evidence used to build molecular models of intrinsically disordered proteins to capture the dynamic behaviour of their polypeptide chains. Such tools include EOM, ASTEROIDS and ENSEMBLE. http://purl.obolibrary.org/obo/ECO_0006340 molecular dynamics evidence http://purl.obolibrary.org/obo/ECO_0007672 computational evidence A type of computational evidence used to study the physical movements of atoms and molecules with empirical molecular mechanics force fields. http://purl.obolibrary.org/obo/ECO_0006341 computational structure modeling evidence http://purl.obolibrary.org/obo/ECO_0005031 structure determination evidence A type of structure determination evidence in which a computational tool is used to generate a model of structure(s) for a molecule. http://purl.obolibrary.org/obo/ECO_0006342 qualitative macroscopy evidence http://purl.obolibrary.org/obo/ECO_0001094 macroscopy evidence A type of macroscopy evidence in which non-numerical observed characteristics or features are captured as text descriptions or categorical assignments. http://purl.obolibrary.org/obo/ECO_0006343 quantitative macroscopy evidence http://purl.obolibrary.org/obo/ECO_0001094 macroscopy evidence A type of macroscopy evidence in which measurement techniques result in numerical data. http://purl.obolibrary.org/obo/ECO_0006344 sporulation assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence that reflects the presence, absence, and/or amount of spore formation. http://purl.obolibrary.org/obo/ECO_0006345 asexual sporulation assay evidence http://purl.obolibrary.org/obo/ECO_0006344 sporulation assay evidence A type of sporulation assay evidence that reflects the presence, absence, and/or amount of asexual spore formation. http://purl.obolibrary.org/obo/ECO_0006346 sexual sporulation assay evidence http://purl.obolibrary.org/obo/ECO_0006344 sporulation assay evidence A type of sporulation assay evidence that reflects the presence, absence, and/or amount of sexual spore formation. http://purl.obolibrary.org/obo/ECO_0006347 host-surrogate penetration assay evidence http://purl.obolibrary.org/obo/ECO_0000006 experimental evidence A type of experimental evidence in which the extent of penetration into a host-surrogate material consisting of a synthetic or other non-living substance is measured. http://purl.obolibrary.org/obo/ECO_0006348 host penetration assay evidence http://purl.obolibrary.org/obo/ECO_0001830 host colonization assay evidence A type of host colonization assay evidence in which the extent of penetration into host tissue is measured. http://purl.obolibrary.org/obo/ECO_0006349 iodine-125-labeled ligand binding evidence http://purl.obolibrary.org/obo/ECO_0007008 radioligand binding assay evidence A type of radioligand binding assay evidence in which iodine-125 label is used to measure receptor-ligand interactions, such as in determining selectivity for a particular ligand. http://purl.obolibrary.org/obo/ECO_0006350 bio-layer interferometry evidence http://purl.obolibrary.org/obo/ECO_0005516 molecule detection assay evidence A type of molecule detection assay evidence in which the detection of the interaction between molecules is done without the use of labels and is instead based on the phase shift-wavelength correlation created between interference patterns off of two unique surfaces on the tip of a biosensor, where the ligand is immobilized onto a bio compatible biosensor while the analyte is in solution. http://purl.obolibrary.org/obo/ECO_0006351 bait-prey interaction evidence http://purl.obolibrary.org/obo/ECO_0000024 protein binding evidence A type of protein binding evidence in which the use of "bait" molecules or proteins allows the purification or identification of a binding partner, the "prey". http://purl.obolibrary.org/obo/ECO_0006352 Congo red staining evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006331 Congo red staining evidence A type of Congo red staining evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006353 Congo red staining evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006331 Congo red staining evidence A type of Congo red staining evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006354 Congo red birefringence evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006352 Congo red staining evidence used in manual assertion A type of Congo red birefringence evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006355 Congo red birefringence evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006353 Congo red staining evidence used in automatic assertion A type of Congo red birefringence evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006356 thioflavin T based fluorescence spectroscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006333 thioflavin T based fluorescence spectroscopy evidence A type of thioflavin T based fluorescence spectroscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006357 thioflavin T based fluorescence spectroscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006333 thioflavin T based fluorescence spectroscopy evidence A type of thioflavin T based fluorescence spectroscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006358 differential scanning fluorimetry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006334 differential scanning fluorimetry evidence A type of differential scanning fluorimetry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006359 differential scanning fluorimetry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006334 differential scanning fluorimetry evidence A type of differential scanning fluorimetry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006360 fluorescence correlation spectroscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006335 fluorescence correlation spectroscopy evidence A type of fluorescence correlation spectroscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006361 fluorescence correlation spectroscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006335 fluorescence correlation spectroscopy evidence A type of fluorescence correlation spectroscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006362 intrinsic fluorescence-based protein conformation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006336 intrinsic fluorescence-based protein conformation evidence A type of intrinsic fluorescence-based protein conformation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006363 intrinsic fluorescence-based protein conformation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006336 intrinsic fluorescence-based protein conformation evidence A type of intrinsic fluorescence-based protein conformation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006364 stopped-flow fluorescence spectroscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006337 stopped-flow fluorescence spectroscopy evidence A type of stopped-flow fluorescence spectroscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006365 stopped-flow fluorescence spectroscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006337 stopped-flow fluorescence spectroscopy evidence A type of stopped-flow fluorescence spectroscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006366 temperature jump relaxation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006338 temperature jump relaxation evidence A type of temperature jump relaxation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006367 temperature jump relaxation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006338 temperature jump relaxation evidence A type of temperature jump relaxation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006368 computational structure modeling evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006341 computational structure modeling evidence A type of computational structure modeling evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006369 computational structure modeling evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006341 computational structure modeling evidence A type of computational structure modeling evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006370 structural ensemble generation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006368 computational structure modeling evidence used in manual assertion A type of structural ensemble generation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006371 structural ensemble generation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006369 computational structure modeling evidence used in automatic assertion A type of structural ensemble generation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006372 molecular dynamics evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006340 molecular dynamics evidence A type of molecular dynamics evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006373 molecular dynamics evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006340 molecular dynamics evidence A type of molecular dynamics evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006374 qualitative macroscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006342 qualitative macroscopy evidence A type of qualitative macroscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006375 qualitative macroscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006342 qualitative macroscopy evidence A type of qualitative macroscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006376 quantitative macroscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006343 quantitative macroscopy evidence A type of quantitative macroscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006377 quantitative macroscopy evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006343 quantitative macroscopy evidence A type of quantitative macroscopy evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006378 sporulation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006344 sporulation assay evidence A type of sporulation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006379 sporulation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006344 sporulation assay evidence A type of sporulation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006380 asexual sporulation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006378 sporulation assay evidence used in manual assertion A type of asexual sporulation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006381 asexual sporulation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006379 sporulation assay evidence used in automatic assertion A type of asexual sporulation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006382 sexual sporulation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006378 sporulation assay evidence used in manual assertion A type of sexual sporulation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006383 sexual sporulation assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006379 sporulation assay evidence used in automatic assertion A type of sexual sporulation assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006384 host-surrogate penetration assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006347 host-surrogate penetration assay evidence A type of host-surrogate penetration assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006385 host-surrogate penetration assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006347 host-surrogate penetration assay evidence A type of host-surrogate penetration assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006386 host penetration assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006348 host penetration assay evidence A type of host penetration assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006387 host penetration assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006348 host penetration assay evidence A type of host penetration assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006388 iodine-125-labeled ligand binding evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006349 iodine-125-labeled ligand binding evidence A type of iodine-125-labeled ligand binding evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006389 iodine-125-labeled ligand binding evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006349 iodine-125-labeled ligand binding evidence A type of iodine-125-labeled ligand binding evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006390 bio-layer interferometry evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006350 bio-layer interferometry evidence A type of bio-layer interferometry evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006391 bio-layer interferometry evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006350 bio-layer interferometry evidence A type of bio-layer interferometry evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006392 bait-prey interaction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006351 bait-prey interaction evidence A type of bait-prey interaction evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006393 bait-prey interaction evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006351 bait-prey interaction evidence A type of bait-prey interaction evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0001546 acetylation assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where acetylated residues are detected in a protein. http://purl.obolibrary.org/obo/ECO_0001547 cleavage assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where the cleavage of a protein into protein fragments by a protease is detected. http://purl.obolibrary.org/obo/ECO_0001548 deacetylation assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where the removal of acetyl groups are detected in a protein. http://purl.obolibrary.org/obo/ECO_0001549 defarnesylation assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where the removal of farnesyl groups from a protein is detected. http://purl.obolibrary.org/obo/ECO_0001550 demethylation assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where the removal of methyl groups from a substrate (RNA/DNA or protein) is detected. http://purl.obolibrary.org/obo/ECO_0001551 desumoylation assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where the removal of sumo groups from a protein is detected. http://purl.obolibrary.org/obo/ECO_0001552 deubiquitination assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where the removal of ubiquitin groups from a protein is detected. http://purl.obolibrary.org/obo/ECO_0001553 farnesylation assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where farnesylated residues in proteins are detected. http://purl.obolibrary.org/obo/ECO_0001554 methylation assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where methylated residues of a substarte (RNA/DNA or protein) are detected. http://purl.obolibrary.org/obo/ECO_0001555 palmitoylation assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where palmitoylated residues in a protein are detected. http://purl.obolibrary.org/obo/ECO_0001556 phosphatase assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where the removal of phosphatase groups from a protein is detected. http://purl.obolibrary.org/obo/ECO_0001557 polyADP-ribosylation assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where ADP-ribosylated residues in proteins are detected. http://purl.obolibrary.org/obo/ECO_0001558 protein kinase assay evidence http://purl.obolibrary.org/obo/ECO_0000005 enzymatic activity assay evidence A type of enzymatic activity assay evidence that measures transfer of a phosphate to a peptide or protein substrate by a protein kinase. http://purl.obolibrary.org/obo/ECO_0001559 sumoylation assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where sumoylated residues on a protein are detected. http://purl.obolibrary.org/obo/ECO_0001561 translation assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where de novo protein synthesis is detected. http://purl.obolibrary.org/obo/ECO_0001562 ubiquitination assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence where ubiquitinated residues on a protein are detected. http://purl.obolibrary.org/obo/ECO_0001563 cell growth assay evidence http://purl.obolibrary.org/obo/ECO_0001565 cell-based assay evidence A type of direct assay evidence where biological cell development, i.e. an increase in cell mass and size are measured. http://purl.obolibrary.org/obo/ECO_0001565 cell-based assay evidence http://purl.obolibrary.org/obo/ECO_0000002 direct assay evidence A type of direct assay evidence resulting from the study of cells. http://purl.obolibrary.org/obo/ECO_0007019 agglutination test evidence http://purl.obolibrary.org/obo/ECO_0000040 immunological assay evidence A type of immunological assay evidence that involves observation of visible clumping of antibody and antigen into a complex. http://purl.obolibrary.org/obo/ECO_0007020 agglutination test evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007019 agglutination test evidence A type of agglutination test evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007021 slide agglutination test evidence http://purl.obolibrary.org/obo/ECO_0007019 agglutination test evidence A type of agglutination test evidence that involves observaiton of visible clumping of antibody and antigen into a complex on a slide. http://purl.obolibrary.org/obo/ECO_0007022 slide agglutination test evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007021 slide agglutination test evidence A type of slide agglutination test evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007023 direct Coombs test evidence http://purl.obolibrary.org/obo/ECO_0007019 agglutination test evidence A type of agglutination test evidence that results in detection of nonagglutinating antibodies or complement proteins on red blood cells in vivo. http://purl.obolibrary.org/obo/ECO_0007024 direct Coombs test evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007023 direct Coombs test evidence A type of direct Coombs test evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007025 indirect Coombs test evidence http://purl.obolibrary.org/obo/ECO_0007019 agglutination test evidence A type of agglutination test evidence that results in screening of antibodies against red blood cell antigens (other than the A and B antigens) that are unbound in a patient's serum in vitro. http://purl.obolibrary.org/obo/ECO_0007026 indirect Coombs test evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007025 indirect Coombs test evidence A type of indirect Coombs test evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007027 direct hemagglutination assay evidence http://purl.obolibrary.org/obo/ECO_0007019 agglutination test evidence A type of agglutination test evidence where some bacteria and viruses cross-link red blood cells and clump together. http://purl.obolibrary.org/obo/ECO_0007028 direct hemagglutination assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007027 direct hemagglutination assay evidence A type of direct hemagglutination assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007029 viral hemagglutination inhibition assay evidence http://purl.obolibrary.org/obo/ECO_0007019 agglutination test evidence A type of agglutination test evidence where antiviral antibodies in a patient's serum or in a lab-produced antiserum neutralize the virus and block it from agglutinating the red blood cells. http://purl.obolibrary.org/obo/ECO_0007030 viral hemagglutination inhibition assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007029 viral hemagglutination inhibition assay evidence A type of viral hemagglutination inhibition assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007031 compement fixation assay evidence http://purl.obolibrary.org/obo/ECO_0000040 immunological assay evidence A type of immunological assay evidence that results in the detection of presence of specific antibodies in the patient's serum based on the use of complement, a biologically labile serum factor. http://purl.obolibrary.org/obo/ECO_0007032 compement fixation assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007031 compement fixation assay evidence A type of compement fixation assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007033 neutralization test assay evidence http://purl.obolibrary.org/obo/ECO_0000040 immunological assay evidence A type of immunogical assay evidence that results in detection of specific neutralizing antibodies in a sample. http://purl.obolibrary.org/obo/ECO_0007034 neutralization test assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007033 neutralization test assay evidence A type of neutralization test assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007035 copper transport assay evidence http://purl.obolibrary.org/obo/ECO_0000134 transport assay evidence A type of transport assay evidence where the rate of copper transport is estimated. http://purl.obolibrary.org/obo/ECO_0007036 copper transport assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007035 copper transport assay evidence A type of copper transport assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007037 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence http://purl.obolibrary.org/obo/ECO_0005019 staining evidence A type of staining evidence that involves the use of the redox dye 5-cyano-2,3-ditolyl tetrazolium chloride to evaluate the respiratory activity of cells. http://purl.obolibrary.org/obo/ECO_0007038 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007037 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence A type of 5-cyano-2,3-ditolyl tetrazolium chloride staining evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007039 plaque assay evidence http://purl.obolibrary.org/obo/ECO_0001563 cell growth assay evidence A type of cell growth assay evidence resulting in direct quantification of infectious virons and antiviral substances through the counting of discrete plaques (infectious units and cellular dead zones) in cell culture. http://purl.obolibrary.org/obo/ECO_0007040 plaque assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007039 plaque assay evidence A type of plaque assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007041 epifluorescence microscopy evidence http://purl.obolibrary.org/obo/ECO_0006271 fluorescence microscopy evidence A type of fluorescence microscopy evidence where the light source is mounted above the specimen and the excitation light passes through the microscope objective lens on its way toward the specimen. http://purl.obolibrary.org/obo/ECO_0007042 epifluorescence microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007041 epifluorescence microscopy evidence A type of epifluorescence microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007043 transmission electron microscopy evidence http://purl.obolibrary.org/obo/ECO_0005033 electron microscopy evidence A type of electron microscopy evidence where a beam of electrons are transmitted through an ultra thin specimen forming an image which is magnified and focused onto an imaging device, such as a fluorescent screen, on a layer of photographic film, or to be detected by a sensor such as a CCD camera. http://purl.obolibrary.org/obo/ECO_0007044 transmission electron microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007043 transmission electron microscopy evidence A type of transmission electron microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007045 scanning electron microscopy evidence http://purl.obolibrary.org/obo/ECO_0005033 electron microscopy evidence A type of electron microscopy evidence where a focused beam of high-energy electrons is used to generate a variety of signals at the surface of solid specimens. http://purl.obolibrary.org/obo/ECO_0007046 scanning electron microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007045 scanning electron microscopy evidence A type of scanning electron microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007047 time-lapsed microscopy evidence http://purl.obolibrary.org/obo/ECO_0001098 microscopy evidence A type of microscopy evidence where a serial images are taken at regular time points to capture the dynamics of what is being observed. http://purl.obolibrary.org/obo/ECO_0007048 time-lapsed microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007047 time-lapsed microscopy evidence A type of time-lapsed microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007049 phase contrast microscopy evidence http://purl.obolibrary.org/obo/ECO_0001098 microscopy evidence A type of microscopy evidence where a transparent specimen is illuminated with visible light and small phase shifts in the light passing through the specimen are used to produce an image. http://purl.obolibrary.org/obo/ECO_0007050 phase contrast microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007049 phase contrast microscopy evidence A type of phase contrast microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007051 transmitted light brightfied mircoscopy evidence http://purl.obolibrary.org/obo/ECO_0001098 microscopy evidence A type of microscopy evidence where a sample is illuminated with transmitted white light from below and observed from above. http://purl.obolibrary.org/obo/ECO_0007052 transmitted light brightfied mircoscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007051 transmitted light brightfied mircoscopy evidence A type of transmitted light brightfied mircoscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007053 koehler illumination microscopy evidence http://purl.obolibrary.org/obo/ECO_0001098 microscopy evidence A type of microscopy evidence that results in a bright image without glare and minimum heating of the specimen by employing both field and an aperture iris diaphragm for illumination. http://purl.obolibrary.org/obo/ECO_0007054 koehler illumination microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007053 koehler illumination microscopy evidence A type of koehler illumination microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007055 differential interference contrast microscopy evidence http://purl.obolibrary.org/obo/ECO_0001098 microscopy evidence A type of microscopy evidence that results in visualization of living cells and transparent specimens by taking advantage of differences in the light refraction of different parts of the specimen. http://purl.obolibrary.org/obo/ECO_0007056 differential interference contrast microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007055 differential interference contrast microscopy evidence A type of differential interference contrast microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007057 extended field laser confocal microscopy evidence http://purl.obolibrary.org/obo/ECO_0005587 confocal microscopy evidence A type of confocal microscopy evidence that results in creation of a continuous confocal multi-colour mosaic from thousands of individually captured images. http://purl.obolibrary.org/obo/ECO_0007058 extended field laser confocal microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007057 extended field laser confocal microscopy evidence A type of extended field laser confocal microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007059 confocal laser scanning microscopy evidence http://purl.obolibrary.org/obo/ECO_0005587 confocal microscopy evidence A type of confocal microscopy evidence that results in an image that is built up pixel-by-pixel by collecting the emitted photons from the fluorophores in the sample by passing a laser beam through a light source aperture which is then focused by an objective lens into a small area on the surface of the sample. http://purl.obolibrary.org/obo/ECO_0007060 confocal laser scanning microscopy evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007059 confocal laser scanning microscopy evidence A type of confocal laser scanning microscopy evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007061 light scattering evidence http://purl.obolibrary.org/obo/ECO_0005031 structure determination evidence A type of structure determination evidence derived from determining the properties of particles in a solution such as size, shape, structure, molecular weight, diffusion and interaction strength by illuminating the sample with a laser beam and the scattered intensity is probed at a certain angle by a detector. http://purl.obolibrary.org/obo/ECO_0007062 light scattering evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007061 light scattering evidence A type of light scattering evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007063 dynamic light scattering assay evidence http://purl.obolibrary.org/obo/ECO_0007061 light scattering evidence A type of light scattering evidence derived by analyzing the fluctuations in the internsity of the scattered light by the particles in motion. http://purl.obolibrary.org/obo/ECO_0007064 dynamic light scattering assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007063 dynamic light scattering assay evidence A type of dynamic light scattering assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007065 static light scattering assay evidence http://purl.obolibrary.org/obo/ECO_0007061 light scattering evidence A type of light scattering evidence derived by measuring the average intensity of scattered light by the particles at multiple angles. http://purl.obolibrary.org/obo/ECO_0007066 static light scattering assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007065 static light scattering assay evidence A type of static light scattering assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007067 colony papillation assay phenotypic evidence http://purl.obolibrary.org/obo/ECO_0007077 colony morphology phenotypic evidence A type of colony morphology phenotypic evidence resulting from the formation of papillae (microcolonies) that protrude outwards from the main colony by mutant cells. http://purl.obolibrary.org/obo/ECO_0007068 colony papillation assay phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007078 colony morphology phenotypic evidence used in manual assertion A type of colony papillation assay phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007069 crystal violet staining evidence http://purl.obolibrary.org/obo/ECO_0005019 staining evidence A type of staining evidence that is derived from the use of gentian violet as a general biological stain and an acid-base indicator. http://purl.obolibrary.org/obo/ECO_0007070 crystal violet staining evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007069 crystal violet staining evidence A type of crystal violet staining evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007071 flow cell biofilm assay evidence http://purl.obolibrary.org/obo/ECO_0006020 biofilm formation assay evidence A type of biofilm formation assay evidence derived from in vitro cultivation and evaluation of bacterial biofilms under hydrodynamic conditions of flow. http://purl.obolibrary.org/obo/ECO_0007072 flow cell biofilm assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007071 flow cell biofilm assay evidence A type of flow cell biofilm assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007073 bacterial 2-hybrid assay evidence http://purl.obolibrary.org/obo/ECO_0000025 bait-prey hybrid interaction evidence A type of bait-prey hybrid interaction evidence that involves the detection of protein-protein interaction by fusing the protein target (the "bait") to RNA polymerase and fusing protein or peptide library to be analyzed (the "prey") to DNA-binding domain. http://purl.obolibrary.org/obo/ECO_0007074 bacterial 2-hybrid assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007073 bacterial 2-hybrid assay evidence A type of bacterial 2-hybrid assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007075 phenomic profiling assay evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence that results from the comparison of phenotype profiles (the physical and biochemical traits of organisms in particular environments) across multiple strains; that is, a meta-analysis of a set of phenotypic patterns from many tests in response to a series of changing genetic and/or environmental factors. http://purl.obolibrary.org/obo/ECO_0007076 phenomic profiling assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007075 phenomic profiling assay evidence A type of phenomic profiling assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007077 colony morphology phenotypic evidence http://purl.obolibrary.org/obo/ECO_0000059 experimental phenotypic evidence A type of experimental phenotypic evidence resulting from studying the physical characteristics of an assemblage of microorganisms growing on a solid surface such as the surface of an agar culture medium. http://purl.obolibrary.org/obo/ECO_0007078 colony morphology phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007077 colony morphology phenotypic evidence A type of colony morphology phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007079 colony color phenotypic evidence http://purl.obolibrary.org/obo/ECO_0007077 colony morphology phenotypic evidence A type of colony morphology evidence resulting from studying the colony pigmentation. http://purl.obolibrary.org/obo/ECO_0007080 colony color phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007079 colony color phenotypic evidence A type of colony color phenotypic evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007081 colony size phenotypic evidence http://purl.obolibrary.org/obo/ECO_0007077 colony morphology phenotypic evidence A type of colony morphology phenotypic evidence resulting from measuring the area or diameter of the colony. http://purl.obolibrary.org/obo/ECO_0007082 colony size phenotypic evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007081 colony size phenotypic evidence A type of colony size phenotypic evidence that is used in an manual assertion. http://purl.obolibrary.org/obo/ECO_0007083 zone of inhibition evidence http://purl.obolibrary.org/obo/ECO_0001563 cell growth assay evidence A type of cell growth assay evidence resulting from assessing the sensitivity or resistance of bacteria or fungi to an antimicrobial agent or chemical by measuring the size of the zone of inhibition that results when the test organism is grown on a solid surface in the presence of the antimicrobial agent or chemical. http://purl.obolibrary.org/obo/ECO_0007084 zone of inhibition evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007083 zone of inhibition evidence A type of zone of inhibition evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007085 Etest evidence http://purl.obolibrary.org/obo/ECO_0007083 zone of inhibition evidence A type of zone of inhibition evidence resulting from testing the antimicrobial susceptibility by using predefined, continuous, and exponential gradient of antibiotic concentrations immobilized along a rectangular plastic test strip. http://purl.obolibrary.org/obo/ECO_0007086 Etest evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007085 Etest evidence A type of Etest evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007088 ribosome profiling evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000270 expression pattern evidence used in manual assertion A type of ribosome profiling evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0001566 quantitative reverse transcription polymerase chain reaction evidence http://purl.obolibrary.org/obo/ECO_0000109 reverse transcription polymerase chain reaction evidence A type of reverse transcription polymerase chain reaction evidence that combines reverse transcription polymerase chain reaction and real time polymerase chain reaction to quantitatively assay for the detection of RNA levels. http://purl.obolibrary.org/obo/ECO_0001567 quantitative reverse transcription polymerase chain reaction evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001566 quantitative reverse transcription polymerase chain reaction evidence A type of quantitative reverse transcription polymerase chain reaction evidence used in manual assertion. http://purl.obolibrary.org/obo/ECO_0001568 quantitative reverse transcription polymerase chain reaction evidence used in automatic assertion. http://purl.obolibrary.org/obo/ECO_0001566 quantitative reverse transcription polymerase chain reaction evidence A type of quantitative reverse transcription polymerase chain reaction evidence used in automatic assertion. http://purl.obolibrary.org/obo/ECO_0006135 quantum mechanics/molecular mechanics simulation evidence http://purl.obolibrary.org/obo/ECO_0007672 computational evidence A type of computational evidence where the active site of the molecular system is described with highly accurate quantum theory, while the contribution of the rest of the system is described with molecular mechanical force field. http://purl.obolibrary.org/obo/ECO_0006136 quantum mechanics/molecular mechanics simulation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006135 quantum mechanics/molecular mechanics simulation evidence A type of quantum mechanics/molecular mechanics simulation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006137 quantum mechanics/molecular mechanics simulation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006135 quantum mechanics/molecular mechanics simulation evidence A type of quantum mechanics/molecular mechanics simulation evidence that is used in manual assertion. http://purl.obolibrary.org/obo/ECO_0006138 molecular mechanics simulation evidence http://purl.obolibrary.org/obo/ECO_0007672 computational evidence A type of computational evidence where the energy of a molecular system is predicted as a function of its conformation. http://purl.obolibrary.org/obo/ECO_0006139 molecular mechanics simulation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006138 molecular mechanics simulation evidence A type of molecular mechanics simulation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006140 molecular mechanics simulation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006138 molecular mechanics simulation evidence A type of molecular mechanics simulation evidence that is used in manual assertion. http://purl.obolibrary.org/obo/ECO_0006141 quantum mechanics simulation evidence http://purl.obolibrary.org/obo/ECO_0007672 computational evidence A type of computational evidence where the behavior of matter and light on the atomic and subatomic scale is described. http://purl.obolibrary.org/obo/ECO_0006142 quantum mechanics simulation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006141 quantum mechanics simulation evidence A type of quantum mechanics simulation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0006143 quantum mechanics simulation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006141 quantum mechanics simulation evidence A type of quantum mechanics simulation evidence that is used in manual assertion. http://purl.obolibrary.org/obo/ECO_0006144 density functional theory simulation evidence http://purl.obolibrary.org/obo/ECO_0006141 quantum mechanics simulation evidence A type of quantum mechanics simulation evidence that results from the calculation of ground-state electronic structure of atoms, molecules and solid state materials. http://purl.obolibrary.org/obo/ECO_0006145 density functional theory simulation evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0006144 density functional theory simulation evidence A type of density functional theory simulation evidence that is used in manual assertion. http://purl.obolibrary.org/obo/ECO_0006146 density functional theory simulation evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0006144 density functional theory simulation evidence A type of density functional theory simulation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007844 radioisotope quantification assay evidence http://purl.obolibrary.org/obo/ECO_0001120 radioisotope assay evidence A type of radioisotope assay evidence in which radioisotopic labeling is used to quantify a substance after a biochemical process or extraction from a biological sample. http://purl.obolibrary.org/obo/ECO_0007845 radioisotope quantification assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007844 radioisotope quantification assay evidence A type of radioisotope quantification assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007846 radioisotope quantification assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007844 radioisotope quantification assay evidence A type of radioisotope quantification assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007847 fluorescence quantification assay evidence http://purl.obolibrary.org/obo/ECO_0001115 fluorescence evidence A type of fluorescence evidence in which fluorescent labeling is used to quantify a substance after a biochemical process or extraction from a biological sample. http://purl.obolibrary.org/obo/ECO_0007848 fluorescence quantification assay evidence used in automatic assertion http://purl.obolibrary.org/obo/ECO_0007847 fluorescence quantification assay evidence A type of fluorescence quantification assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007849 fluorescence quantification assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0007847 fluorescence quantification assay evidence A type of fluorescence quantification assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0006303 microscale thermophoresis evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0001249 fluorescence evidence used in manual assertion A type of microscale thermophoresis evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/CHEBI_23528 cytochalasin http://purl.obolibrary.org/obo/CHEBI_23367 molecular entity http://purl.obolibrary.org/obo/BFO_0000141 immaterial entity http://purl.obolibrary.org/obo/BFO_0000004 independent continuant http://purl.obolibrary.org/obo/BFO_0000034 function http://purl.obolibrary.org/obo/BFO_0000016 disposition http://purl.obolibrary.org/obo/BFO_0000016 disposition http://purl.obolibrary.org/obo/BFO_0000017 realizable entity http://purl.obolibrary.org/obo/BFO_0000019 quality http://purl.obolibrary.org/obo/BFO_0000020 specifically dependent continuant http://purl.obolibrary.org/obo/BFO_0000006 spatial region http://purl.obolibrary.org/obo/BFO_0000141 immaterial entity http://purl.obolibrary.org/obo/BFO_0000029 site http://purl.obolibrary.org/obo/BFO_0000141 immaterial entity http://purl.obolibrary.org/obo/BFO_0000140 continuant fiat boundary http://purl.obolibrary.org/obo/BFO_0000141 immaterial entity http://purl.obolibrary.org/obo/NCBITaxon_10239 Viruses http://purl.obolibrary.org/obo/OBI_0100026 organism http://purl.obolibrary.org/obo/NCBITaxon_2157 Archaea http://purl.obolibrary.org/obo/OBI_0100026 organism http://purl.obolibrary.org/obo/NCBITaxon_2 Bacteria http://purl.obolibrary.org/obo/OBI_0100026 organism http://purl.obolibrary.org/obo/NCBITaxon_2759 Eukaryota http://purl.obolibrary.org/obo/OBI_0100026 organism http://purl.obolibrary.org/obo/GO_0120031 plasma membrane bounded cell projection assembly http://purl.obolibrary.org/obo/GO_0030031 cell projection assembly http://purl.obolibrary.org/obo/CHEBI_4705 double-stranded DNA http://purl.obolibrary.org/obo/CHEBI_16991 deoxyribonucleic acid http://purl.obolibrary.org/obo/GO_0004519 endonuclease activity http://purl.obolibrary.org/obo/GO_0004518 nuclease activity http://purl.obolibrary.org/obo/GO_0004536 deoxyribonuclease activity http://purl.obolibrary.org/obo/GO_0140097 catalytic activity, acting on DNA http://purl.obolibrary.org/obo/GO_0004520 endodeoxyribonuclease activity http://purl.obolibrary.org/obo/GO_0004536 deoxyribonuclease activity http://purl.obolibrary.org/obo/GO_0022857 transmembrane transporter activity http://purl.obolibrary.org/obo/GO_0005215 transporter activity http://purl.obolibrary.org/obo/GO_0110165 cellular anatomical entity http://purl.obolibrary.org/obo/GO_0005575 cellular_component http://purl.obolibrary.org/obo/GO_0034655 nucleobase-containing compound catabolic process http://purl.obolibrary.org/obo/GO_1901361 organic cyclic compound catabolic process http://purl.obolibrary.org/obo/GO_0090304 nucleic acid metabolic process http://purl.obolibrary.org/obo/GO_0043170 macromolecule metabolic process http://purl.obolibrary.org/obo/GO_0034654 nucleobase-containing compound biosynthetic process http://purl.obolibrary.org/obo/GO_1901362 organic cyclic compound biosynthetic process http://purl.obolibrary.org/obo/GO_0008156 negative regulation of DNA replication http://purl.obolibrary.org/obo/GO_2000113 negative regulation of cellular macromolecule biosynthetic process http://purl.obolibrary.org/obo/GO_0045740 positive regulation of DNA replication http://purl.obolibrary.org/obo/GO_0031328 positive regulation of cellular biosynthetic process http://purl.obolibrary.org/obo/GO_0006305 DNA alkylation http://purl.obolibrary.org/obo/GO_0006304 DNA modification http://purl.obolibrary.org/obo/GO_0044728 DNA methylation or demethylation http://purl.obolibrary.org/obo/GO_0006304 DNA modification http://purl.obolibrary.org/obo/GO_0006448 regulation of translational elongation http://purl.obolibrary.org/obo/GO_0006417 regulation of translation http://purl.obolibrary.org/obo/GO_0017148 negative regulation of translation http://purl.obolibrary.org/obo/GO_0032269 negative regulation of cellular protein metabolic process http://purl.obolibrary.org/obo/GO_0043555 regulation of translation in response to stress http://purl.obolibrary.org/obo/GO_0006417 regulation of translation http://purl.obolibrary.org/obo/GO_0043143 regulation of translation by machinery localization http://purl.obolibrary.org/obo/GO_0034613 cellular protein localization http://purl.obolibrary.org/obo/GO_0045727 positive regulation of translation http://purl.obolibrary.org/obo/GO_0010628 positive regulation of gene expression http://purl.obolibrary.org/obo/GO_0045900 negative regulation of translational elongation http://purl.obolibrary.org/obo/GO_0017148 negative regulation of translation http://purl.obolibrary.org/obo/GO_0045901 positive regulation of translational elongation http://purl.obolibrary.org/obo/GO_0045727 positive regulation of translation http://purl.obolibrary.org/obo/GO_0043043 peptide biosynthetic process http://purl.obolibrary.org/obo/GO_1901566 organonitrogen compound biosynthetic process http://purl.obolibrary.org/obo/GO_0006139 nucleobase-containing compound metabolic process http://purl.obolibrary.org/obo/GO_1901360 organic cyclic compound metabolic process http://purl.obolibrary.org/obo/GO_0019439 aromatic compound catabolic process http://purl.obolibrary.org/obo/GO_0044248 cellular catabolic process http://purl.obolibrary.org/obo/GO_0019438 aromatic compound biosynthetic process http://purl.obolibrary.org/obo/GO_0044249 cellular biosynthetic process http://purl.obolibrary.org/obo/GO_0034641 cellular nitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0044237 cellular metabolic process http://purl.obolibrary.org/obo/GO_1901564 organonitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0071704 organic substance metabolic process http://purl.obolibrary.org/obo/GO_0006811 ion transport http://purl.obolibrary.org/obo/GO_0006810 transport http://purl.obolibrary.org/obo/GO_0055085 transmembrane transport http://purl.obolibrary.org/obo/GO_0009987 cellular process http://purl.obolibrary.org/obo/GO_0071702 organic substance transport http://purl.obolibrary.org/obo/GO_0006810 transport http://purl.obolibrary.org/obo/GO_0071705 nitrogen compound transport http://purl.obolibrary.org/obo/GO_0006810 transport http://purl.obolibrary.org/obo/GO_0046903 secretion http://purl.obolibrary.org/obo/GO_0006810 transport http://purl.obolibrary.org/obo/GO_0006820 anion transport http://purl.obolibrary.org/obo/GO_0006811 ion transport http://purl.obolibrary.org/obo/GO_0034220 ion transmembrane transport http://purl.obolibrary.org/obo/GO_0055085 transmembrane transport http://purl.obolibrary.org/obo/GO_0015711 organic anion transport http://purl.obolibrary.org/obo/GO_0071702 organic substance transport http://purl.obolibrary.org/obo/GO_0030262 apoptotic nuclear changes http://purl.obolibrary.org/obo/GO_0006921 cellular component disassembly involved in execution phase of apoptosis http://purl.obolibrary.org/obo/GO_0048870 cell motility http://purl.obolibrary.org/obo/GO_0040011 locomotion http://purl.obolibrary.org/obo/GO_0060326 cell chemotaxis http://purl.obolibrary.org/obo/GO_0016477 cell migration http://purl.obolibrary.org/obo/GO_0006997 nucleus organization http://purl.obolibrary.org/obo/GO_0006996 organelle organization http://purl.obolibrary.org/obo/GO_0007576 nucleolar fragmentation http://purl.obolibrary.org/obo/GO_0007000 nucleolus organization http://purl.obolibrary.org/obo/GO_0007569 cell aging http://purl.obolibrary.org/obo/GO_0048869 cellular developmental process http://purl.obolibrary.org/obo/GO_0034613 cellular protein localization http://purl.obolibrary.org/obo/GO_0070727 cellular macromolecule localization http://purl.obolibrary.org/obo/GO_0045184 establishment of protein localization http://purl.obolibrary.org/obo/GO_0051234 establishment of localization http://purl.obolibrary.org/obo/GO_0006807 nitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0008152 metabolic process http://purl.obolibrary.org/obo/GO_0032259 methylation http://purl.obolibrary.org/obo/GO_0008152 metabolic process http://purl.obolibrary.org/obo/GO_0044238 primary metabolic process http://purl.obolibrary.org/obo/GO_0008152 metabolic process http://purl.obolibrary.org/obo/GO_0071704 organic substance metabolic process http://purl.obolibrary.org/obo/GO_0008152 metabolic process http://purl.obolibrary.org/obo/GO_0009058 biosynthetic process http://purl.obolibrary.org/obo/GO_0008152 metabolic process http://purl.obolibrary.org/obo/GO_0044237 cellular metabolic process http://purl.obolibrary.org/obo/GO_0009987 cellular process http://purl.obolibrary.org/obo/GO_0009056 catabolic process http://purl.obolibrary.org/obo/GO_0008152 metabolic process http://purl.obolibrary.org/obo/GO_0006417 regulation of translation http://purl.obolibrary.org/obo/GO_0034248 regulation of cellular amide metabolic process http://purl.obolibrary.org/obo/GO_0016441 posttranscriptional gene silencing http://purl.obolibrary.org/obo/GO_0010629 negative regulation of gene expression http://purl.obolibrary.org/obo/GO_0001818 negative regulation of cytokine production http://purl.obolibrary.org/obo/GO_0051241 negative regulation of multicellular organismal process http://purl.obolibrary.org/obo/GO_0031047 gene silencing by RNA http://purl.obolibrary.org/obo/GO_0010629 negative regulation of gene expression http://purl.obolibrary.org/obo/GO_1903963 arachidonate transport http://purl.obolibrary.org/obo/GO_0071715 icosanoid transport http://purl.obolibrary.org/obo/GO_0006996 organelle organization http://purl.obolibrary.org/obo/GO_0016043 cellular component organization http://purl.obolibrary.org/obo/GO_0022411 cellular component disassembly http://purl.obolibrary.org/obo/GO_0016043 cellular component organization http://purl.obolibrary.org/obo/GO_0022607 cellular component assembly http://purl.obolibrary.org/obo/GO_0016043 cellular component organization http://purl.obolibrary.org/obo/GO_0032989 cellular component morphogenesis http://purl.obolibrary.org/obo/GO_0048869 cellular developmental process http://purl.obolibrary.org/obo/GO_0030030 cell projection organization http://purl.obolibrary.org/obo/GO_0016043 cellular component organization http://purl.obolibrary.org/obo/GO_0004518 nuclease activity http://purl.obolibrary.org/obo/GO_0016788 hydrolase activity, acting on ester bonds http://purl.obolibrary.org/obo/GO_0032055 negative regulation of translation in response to stress http://purl.obolibrary.org/obo/GO_0043555 regulation of translation in response to stress http://purl.obolibrary.org/obo/GO_0040033 RNA-mediated gene silencing by inhibition of translation http://purl.obolibrary.org/obo/GO_0035194 post-transcriptional gene silencing by RNA http://purl.obolibrary.org/obo/GO_0051052 regulation of DNA metabolic process http://purl.obolibrary.org/obo/GO_0060255 regulation of macromolecule metabolic process http://purl.obolibrary.org/obo/GO_0051252 regulation of RNA metabolic process http://purl.obolibrary.org/obo/GO_0060255 regulation of macromolecule metabolic process http://purl.obolibrary.org/obo/GO_0045935 positive regulation of nucleobase-containing compound metabolic process http://purl.obolibrary.org/obo/GO_0051173 positive regulation of nitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0045934 negative regulation of nucleobase-containing compound metabolic process http://purl.obolibrary.org/obo/GO_0051172 negative regulation of nitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0044267 cellular protein metabolic process http://purl.obolibrary.org/obo/GO_0044260 cellular macromolecule metabolic process http://purl.obolibrary.org/obo/GO_0006921 cellular component disassembly involved in execution phase of apoptosis http://purl.obolibrary.org/obo/GO_0022411 cellular component disassembly http://purl.obolibrary.org/obo/GO_0015075 ion transmembrane transporter activity http://purl.obolibrary.org/obo/GO_0022857 transmembrane transporter activity http://purl.obolibrary.org/obo/GO_0015318 inorganic molecular entity transmembrane transporter activity http://purl.obolibrary.org/obo/GO_0022857 transmembrane transporter activity http://purl.obolibrary.org/obo/GO_0022803 passive transmembrane transporter activity http://purl.obolibrary.org/obo/GO_0022857 transmembrane transporter activity http://purl.obolibrary.org/obo/GO_0120033 negative regulation of plasma membrane bounded cell projection assembly http://purl.obolibrary.org/obo/GO_0120032 regulation of plasma membrane bounded cell projection assembly http://purl.obolibrary.org/obo/GO_0032077 positive regulation of deoxyribonuclease activity http://purl.obolibrary.org/obo/GO_0051054 positive regulation of DNA metabolic process http://purl.obolibrary.org/obo/GO_0043414 macromolecule methylation http://purl.obolibrary.org/obo/GO_0044260 cellular macromolecule metabolic process http://purl.obolibrary.org/obo/GO_0032270 positive regulation of cellular protein metabolic process http://purl.obolibrary.org/obo/GO_0051247 positive regulation of protein metabolic process http://purl.obolibrary.org/obo/GO_0032269 negative regulation of cellular protein metabolic process http://purl.obolibrary.org/obo/GO_0051248 negative regulation of protein metabolic process http://purl.obolibrary.org/obo/GO_0032369 negative regulation of lipid transport http://purl.obolibrary.org/obo/GO_1905953 negative regulation of lipid localization http://purl.obolibrary.org/obo/GO_0032370 positive regulation of lipid transport http://purl.obolibrary.org/obo/GO_1905954 positive regulation of lipid localization http://purl.obolibrary.org/obo/GO_2000191 regulation of fatty acid transport http://purl.obolibrary.org/obo/GO_0032890 regulation of organic acid transport http://purl.obolibrary.org/obo/GO_0008104 protein localization http://purl.obolibrary.org/obo/GO_0033036 macromolecule localization http://purl.obolibrary.org/obo/GO_0010876 lipid localization http://purl.obolibrary.org/obo/GO_0033036 macromolecule localization http://purl.obolibrary.org/obo/GO_0070727 cellular macromolecule localization http://purl.obolibrary.org/obo/GO_0051641 cellular localization http://purl.obolibrary.org/obo/GO_0042277 peptide binding http://purl.obolibrary.org/obo/GO_0033218 amide binding http://purl.obolibrary.org/obo/GO_0044270 cellular nitrogen compound catabolic process http://purl.obolibrary.org/obo/GO_0044248 cellular catabolic process http://purl.obolibrary.org/obo/GO_0043603 cellular amide metabolic process http://purl.obolibrary.org/obo/GO_0034641 cellular nitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0044271 cellular nitrogen compound biosynthetic process http://purl.obolibrary.org/obo/GO_0044249 cellular biosynthetic process http://purl.obolibrary.org/obo/GO_0006260 DNA replication http://purl.obolibrary.org/obo/GO_0034645 cellular macromolecule biosynthetic process http://purl.obolibrary.org/obo/GO_0006412 translation http://purl.obolibrary.org/obo/GO_0043043 peptide biosynthetic process http://purl.obolibrary.org/obo/GO_0006414 translational elongation http://purl.obolibrary.org/obo/GO_0034645 cellular macromolecule biosynthetic process http://purl.obolibrary.org/obo/GO_0071897 DNA biosynthetic process http://purl.obolibrary.org/obo/GO_0034654 nucleobase-containing compound biosynthetic process http://purl.obolibrary.org/obo/GO_0006308 DNA catabolic process http://purl.obolibrary.org/obo/GO_0006259 DNA metabolic process http://purl.obolibrary.org/obo/GO_0042868 antisense RNA metabolic process http://purl.obolibrary.org/obo/GO_0034660 ncRNA metabolic process http://purl.obolibrary.org/obo/GO_0034766 negative regulation of ion transmembrane transport http://purl.obolibrary.org/obo/GO_0043271 negative regulation of ion transport http://purl.obolibrary.org/obo/GO_0034767 positive regulation of ion transmembrane transport http://purl.obolibrary.org/obo/GO_0043270 positive regulation of ion transport http://purl.obolibrary.org/obo/GO_0032412 regulation of ion transmembrane transporter activity http://purl.obolibrary.org/obo/GO_0022898 regulation of transmembrane transporter activity http://purl.obolibrary.org/obo/GO_1904746 negative regulation of apoptotic process involved in development http://purl.obolibrary.org/obo/GO_1904748 regulation of apoptotic process involved in development http://purl.obolibrary.org/obo/GO_1900118 negative regulation of execution phase of apoptosis http://purl.obolibrary.org/obo/GO_1900117 regulation of execution phase of apoptosis http://purl.obolibrary.org/obo/GO_0051345 positive regulation of hydrolase activity http://purl.obolibrary.org/obo/GO_0051336 regulation of hydrolase activity http://purl.obolibrary.org/obo/GO_0051346 negative regulation of hydrolase activity http://purl.obolibrary.org/obo/GO_0051336 regulation of hydrolase activity http://purl.obolibrary.org/obo/GO_0019538 protein metabolic process http://purl.obolibrary.org/obo/GO_1901564 organonitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0043412 macromolecule modification http://purl.obolibrary.org/obo/GO_0043170 macromolecule metabolic process http://purl.obolibrary.org/obo/GO_0044260 cellular macromolecule metabolic process http://purl.obolibrary.org/obo/GO_0044237 cellular metabolic process http://purl.obolibrary.org/obo/GO_0009057 macromolecule catabolic process http://purl.obolibrary.org/obo/GO_1901575 organic substance catabolic process http://purl.obolibrary.org/obo/GO_0009059 macromolecule biosynthetic process http://purl.obolibrary.org/obo/GO_1901576 organic substance biosynthetic process http://purl.obolibrary.org/obo/GO_0006304 DNA modification http://purl.obolibrary.org/obo/GO_0006259 DNA metabolic process http://purl.obolibrary.org/obo/GO_0032303 regulation of icosanoid secretion http://purl.obolibrary.org/obo/GO_2000191 regulation of fatty acid transport http://purl.obolibrary.org/obo/GO_1903792 negative regulation of anion transport http://purl.obolibrary.org/obo/GO_0043271 negative regulation of ion transport http://purl.obolibrary.org/obo/GO_1903793 positive regulation of anion transport http://purl.obolibrary.org/obo/GO_0043270 positive regulation of ion transport http://purl.obolibrary.org/obo/GO_0044089 positive regulation of cellular component biogenesis http://purl.obolibrary.org/obo/GO_0048522 positive regulation of cellular process http://purl.obolibrary.org/obo/GO_0060491 regulation of cell projection assembly http://purl.obolibrary.org/obo/GO_0031344 regulation of cell projection organization http://purl.obolibrary.org/obo/GO_0044265 cellular macromolecule catabolic process http://purl.obolibrary.org/obo/GO_0009057 macromolecule catabolic process http://purl.obolibrary.org/obo/GO_0046700 heterocycle catabolic process http://purl.obolibrary.org/obo/GO_0046483 heterocycle metabolic process http://purl.obolibrary.org/obo/GO_0034645 cellular macromolecule biosynthetic process http://purl.obolibrary.org/obo/GO_0009059 macromolecule biosynthetic process http://purl.obolibrary.org/obo/GO_0018130 heterocycle biosynthetic process http://purl.obolibrary.org/obo/GO_0046483 heterocycle metabolic process http://purl.obolibrary.org/obo/GO_0006259 DNA metabolic process http://purl.obolibrary.org/obo/GO_0090304 nucleic acid metabolic process http://purl.obolibrary.org/obo/GO_0015718 monocarboxylic acid transport http://purl.obolibrary.org/obo/GO_0046942 carboxylic acid transport http://purl.obolibrary.org/obo/GO_0071715 icosanoid transport http://purl.obolibrary.org/obo/GO_0046942 carboxylic acid transport http://purl.obolibrary.org/obo/GO_0031324 negative regulation of cellular metabolic process http://purl.obolibrary.org/obo/GO_0009892 negative regulation of metabolic process http://purl.obolibrary.org/obo/GO_0031342 negative regulation of cell killing http://purl.obolibrary.org/obo/GO_0031341 regulation of cell killing http://purl.obolibrary.org/obo/GO_0051271 negative regulation of cellular component movement http://purl.obolibrary.org/obo/GO_0051270 regulation of cellular component movement http://purl.obolibrary.org/obo/GO_0030308 negative regulation of cell growth http://purl.obolibrary.org/obo/GO_0045926 negative regulation of growth http://purl.obolibrary.org/obo/GO_0034763 negative regulation of transmembrane transport http://purl.obolibrary.org/obo/GO_0051051 negative regulation of transport http://purl.obolibrary.org/obo/GO_0060548 negative regulation of cell death http://purl.obolibrary.org/obo/GO_0010941 regulation of cell death http://purl.obolibrary.org/obo/GO_2001021 negative regulation of response to DNA damage stimulus http://purl.obolibrary.org/obo/GO_0048585 negative regulation of response to stimulus http://purl.obolibrary.org/obo/GO_0090344 negative regulation of cell aging http://purl.obolibrary.org/obo/GO_0090342 regulation of cell aging http://purl.obolibrary.org/obo/GO_0008285 negative regulation of cell population proliferation http://purl.obolibrary.org/obo/GO_0042127 regulation of cell population proliferation http://purl.obolibrary.org/obo/GO_0051129 negative regulation of cellular component organization http://purl.obolibrary.org/obo/GO_0051128 regulation of cellular component organization http://purl.obolibrary.org/obo/GO_0060354 negative regulation of cell adhesion molecule production http://purl.obolibrary.org/obo/GO_0060353 regulation of cell adhesion molecule production http://purl.obolibrary.org/obo/GO_0051716 cellular response to stimulus http://purl.obolibrary.org/obo/GO_0009987 cellular process http://purl.obolibrary.org/obo/GO_0006950 response to stress http://purl.obolibrary.org/obo/GO_0050896 response to stimulus http://purl.obolibrary.org/obo/GO_0009605 response to external stimulus http://purl.obolibrary.org/obo/GO_0050896 response to stimulus http://purl.obolibrary.org/obo/GO_0009607 response to biotic stimulus http://purl.obolibrary.org/obo/GO_0050896 response to stimulus http://purl.obolibrary.org/obo/GO_0042221 response to chemical http://purl.obolibrary.org/obo/GO_0050896 response to stimulus http://purl.obolibrary.org/obo/GO_0009719 response to endogenous stimulus http://purl.obolibrary.org/obo/GO_0050896 response to stimulus http://purl.obolibrary.org/obo/GO_0032076 negative regulation of deoxyribonuclease activity http://purl.obolibrary.org/obo/GO_0032074 negative regulation of nuclease activity http://purl.obolibrary.org/obo/GO_2000279 negative regulation of DNA biosynthetic process http://purl.obolibrary.org/obo/GO_2000278 regulation of DNA biosynthetic process http://purl.obolibrary.org/obo/GO_1903625 negative regulation of DNA catabolic process http://purl.obolibrary.org/obo/GO_0031330 negative regulation of cellular catabolic process http://purl.obolibrary.org/obo/GO_1905642 negative regulation of DNA methylation http://purl.obolibrary.org/obo/GO_0044030 regulation of DNA methylation http://purl.obolibrary.org/obo/GO_0006810 transport http://purl.obolibrary.org/obo/GO_0051234 establishment of localization http://purl.obolibrary.org/obo/GO_0033554 cellular response to stress http://purl.obolibrary.org/obo/GO_0006950 response to stress http://purl.obolibrary.org/obo/GO_0070887 cellular response to chemical stimulus http://purl.obolibrary.org/obo/GO_0042221 response to chemical http://purl.obolibrary.org/obo/GO_0015031 protein transport http://purl.obolibrary.org/obo/GO_0045184 establishment of protein localization http://purl.obolibrary.org/obo/GO_0006869 lipid transport http://purl.obolibrary.org/obo/GO_0071702 organic substance transport http://purl.obolibrary.org/obo/GO_0015849 organic acid transport http://purl.obolibrary.org/obo/GO_0071702 organic substance transport http://purl.obolibrary.org/obo/GO_0043170 macromolecule metabolic process http://purl.obolibrary.org/obo/GO_0071704 organic substance metabolic process http://purl.obolibrary.org/obo/GO_1901575 organic substance catabolic process http://purl.obolibrary.org/obo/GO_0009056 catabolic process http://purl.obolibrary.org/obo/GO_1901576 organic substance biosynthetic process http://purl.obolibrary.org/obo/GO_0009058 biosynthetic process http://purl.obolibrary.org/obo/GO_1901360 organic cyclic compound metabolic process http://purl.obolibrary.org/obo/GO_0071704 organic substance metabolic process http://purl.obolibrary.org/obo/GO_0019219 regulation of nucleobase-containing compound metabolic process http://purl.obolibrary.org/obo/GO_0051171 regulation of nitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0051246 regulation of protein metabolic process http://purl.obolibrary.org/obo/GO_0051171 regulation of nitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0016070 RNA metabolic process http://purl.obolibrary.org/obo/GO_0090304 nucleic acid metabolic process http://purl.obolibrary.org/obo/GO_0090305 nucleic acid phosphodiester bond hydrolysis http://purl.obolibrary.org/obo/GO_0090304 nucleic acid metabolic process http://purl.obolibrary.org/obo/GO_1901361 organic cyclic compound catabolic process http://purl.obolibrary.org/obo/GO_1901360 organic cyclic compound metabolic process http://purl.obolibrary.org/obo/GO_1901362 organic cyclic compound biosynthetic process http://purl.obolibrary.org/obo/GO_1901360 organic cyclic compound metabolic process http://purl.obolibrary.org/obo/GO_1901566 organonitrogen compound biosynthetic process http://purl.obolibrary.org/obo/GO_1901564 organonitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0006275 regulation of DNA replication http://purl.obolibrary.org/obo/GO_2000112 regulation of cellular macromolecule biosynthetic process http://purl.obolibrary.org/obo/GO_2000113 negative regulation of cellular macromolecule biosynthetic process http://purl.obolibrary.org/obo/GO_0031327 negative regulation of cellular biosynthetic process http://purl.obolibrary.org/obo/GO_2000278 regulation of DNA biosynthetic process http://purl.obolibrary.org/obo/GO_0051052 regulation of DNA metabolic process http://purl.obolibrary.org/obo/GO_2001022 positive regulation of response to DNA damage stimulus http://purl.obolibrary.org/obo/GO_0048522 positive regulation of cellular process http://purl.obolibrary.org/obo/GO_0051272 positive regulation of cellular component movement http://purl.obolibrary.org/obo/GO_0048522 positive regulation of cellular process http://purl.obolibrary.org/obo/GO_2000145 regulation of cell motility http://purl.obolibrary.org/obo/GO_0040012 regulation of locomotion http://purl.obolibrary.org/obo/GO_0050789 regulation of biological process http://purl.obolibrary.org/obo/GO_0065007 biological regulation http://purl.obolibrary.org/obo/GO_0065008 regulation of biological quality http://purl.obolibrary.org/obo/GO_0065007 biological regulation http://purl.obolibrary.org/obo/GO_0065009 regulation of molecular function http://purl.obolibrary.org/obo/GO_0065007 biological regulation http://purl.obolibrary.org/obo/GO_1904950 negative regulation of establishment of protein localization http://purl.obolibrary.org/obo/GO_1903828 negative regulation of protein localization http://purl.obolibrary.org/obo/GO_1904951 positive regulation of establishment of protein localization http://purl.obolibrary.org/obo/GO_1903829 positive regulation of protein localization http://purl.obolibrary.org/obo/GO_0051223 regulation of protein transport http://purl.obolibrary.org/obo/GO_0051049 regulation of transport http://purl.obolibrary.org/obo/GO_1903508 positive regulation of nucleic acid-templated transcription http://purl.obolibrary.org/obo/GO_1903506 regulation of nucleic acid-templated transcription http://purl.obolibrary.org/obo/GO_1903035 negative regulation of response to wounding http://purl.obolibrary.org/obo/GO_0048585 negative regulation of response to stimulus http://purl.obolibrary.org/obo/GO_1903036 positive regulation of response to wounding http://purl.obolibrary.org/obo/GO_0048584 positive regulation of response to stimulus http://purl.obolibrary.org/obo/GO_0061041 regulation of wound healing http://purl.obolibrary.org/obo/GO_1903034 regulation of response to wounding http://purl.obolibrary.org/obo/GO_0061045 negative regulation of wound healing http://purl.obolibrary.org/obo/GO_0032102 negative regulation of response to external stimulus http://purl.obolibrary.org/obo/GO_0090303 positive regulation of wound healing http://purl.obolibrary.org/obo/GO_0061041 regulation of wound healing http://purl.obolibrary.org/obo/GO_0045893 positive regulation of transcription, DNA-templated http://purl.obolibrary.org/obo/GO_0006355 regulation of transcription, DNA-templated http://purl.obolibrary.org/obo/GO_0050482 arachidonic acid secretion http://purl.obolibrary.org/obo/GO_0032309 icosanoid secretion http://purl.obolibrary.org/obo/GO_0051224 negative regulation of protein transport http://purl.obolibrary.org/obo/GO_0051051 negative regulation of transport http://purl.obolibrary.org/obo/GO_0051222 positive regulation of protein transport http://purl.obolibrary.org/obo/GO_0051050 positive regulation of transport http://purl.obolibrary.org/obo/GO_1904171 negative regulation of bleb assembly http://purl.obolibrary.org/obo/GO_0120033 negative regulation of plasma membrane bounded cell projection assembly http://purl.obolibrary.org/obo/GO_1904172 positive regulation of bleb assembly http://purl.obolibrary.org/obo/GO_0120034 positive regulation of plasma membrane bounded cell projection assembly http://purl.obolibrary.org/obo/GO_1904797 negative regulation of core promoter binding http://purl.obolibrary.org/obo/GO_2000678 negative regulation of transcription regulatory region DNA binding http://purl.obolibrary.org/obo/GO_1904798 positive regulation of core promoter binding http://purl.obolibrary.org/obo/GO_2000679 positive regulation of transcription regulatory region DNA binding http://purl.obolibrary.org/obo/GO_0006309 apoptotic DNA fragmentation http://purl.obolibrary.org/obo/GO_0000737 DNA catabolic process, endonucleolytic http://purl.obolibrary.org/obo/GO_0000976 transcription cis-regulatory region binding http://purl.obolibrary.org/obo/GO_1990837 sequence-specific double-stranded DNA binding http://purl.obolibrary.org/obo/GO_0030307 positive regulation of cell growth http://purl.obolibrary.org/obo/GO_0048522 positive regulation of cellular process http://purl.obolibrary.org/obo/GO_0071706 tumor necrosis factor superfamily cytokine production http://purl.obolibrary.org/obo/GO_0001816 cytokine production http://purl.obolibrary.org/obo/GO_0001819 positive regulation of cytokine production http://purl.obolibrary.org/obo/GO_0010628 positive regulation of gene expression http://purl.obolibrary.org/obo/GO_1903555 regulation of tumor necrosis factor superfamily cytokine production http://purl.obolibrary.org/obo/GO_0001817 regulation of cytokine production http://purl.obolibrary.org/obo/GO_1903556 negative regulation of tumor necrosis factor superfamily cytokine production http://purl.obolibrary.org/obo/GO_1903555 regulation of tumor necrosis factor superfamily cytokine production http://purl.obolibrary.org/obo/GO_1903557 positive regulation of tumor necrosis factor superfamily cytokine production http://purl.obolibrary.org/obo/GO_1903555 regulation of tumor necrosis factor superfamily cytokine production http://purl.obolibrary.org/obo/GO_0032549 ribonucleoside binding http://purl.obolibrary.org/obo/GO_0001882 nucleoside binding http://purl.obolibrary.org/obo/GO_0002832 negative regulation of response to biotic stimulus http://purl.obolibrary.org/obo/GO_0048585 negative regulation of response to stimulus http://purl.obolibrary.org/obo/GO_0002833 positive regulation of response to biotic stimulus http://purl.obolibrary.org/obo/GO_0048584 positive regulation of response to stimulus http://purl.obolibrary.org/obo/GO_0005215 transporter activity http://purl.obolibrary.org/obo/GO_0003674 molecular_function http://purl.obolibrary.org/obo/GO_0005488 binding http://purl.obolibrary.org/obo/GO_0003674 molecular_function http://purl.obolibrary.org/obo/GO_0045182 translation regulator activity http://purl.obolibrary.org/obo/GO_0003674 molecular_function http://purl.obolibrary.org/obo/GO_0001067 transcription regulatory region nucleic acid binding http://purl.obolibrary.org/obo/GO_0003676 nucleic acid binding http://purl.obolibrary.org/obo/GO_0003677 DNA binding http://purl.obolibrary.org/obo/GO_0003676 nucleic acid binding http://purl.obolibrary.org/obo/GO_0003690 double-stranded DNA binding http://purl.obolibrary.org/obo/GO_0003677 DNA binding http://purl.obolibrary.org/obo/GO_1990837 sequence-specific double-stranded DNA binding http://purl.obolibrary.org/obo/GO_0043565 sequence-specific DNA binding http://purl.obolibrary.org/obo/GO_0042605 peptide antigen binding http://purl.obolibrary.org/obo/GO_0042277 peptide binding http://purl.obolibrary.org/obo/GO_0016787 hydrolase activity http://purl.obolibrary.org/obo/GO_0003824 catalytic activity http://purl.obolibrary.org/obo/GO_0140640 catalytic activity, acting on a nucleic acid http://purl.obolibrary.org/obo/GO_0003824 catalytic activity http://purl.obolibrary.org/obo/GO_0033218 amide binding http://purl.obolibrary.org/obo/GO_0005488 binding http://purl.obolibrary.org/obo/GO_0003823 antigen binding http://purl.obolibrary.org/obo/GO_0005488 binding http://purl.obolibrary.org/obo/GO_0005515 protein binding http://purl.obolibrary.org/obo/GO_0005488 binding http://purl.obolibrary.org/obo/GO_0008289 lipid binding http://purl.obolibrary.org/obo/GO_0005488 binding http://purl.obolibrary.org/obo/GO_0036094 small molecule binding http://purl.obolibrary.org/obo/GO_0005488 binding http://purl.obolibrary.org/obo/GO_0097159 organic cyclic compound binding http://purl.obolibrary.org/obo/GO_0005488 binding http://purl.obolibrary.org/obo/GO_0097367 carbohydrate derivative binding http://purl.obolibrary.org/obo/GO_0005488 binding http://purl.obolibrary.org/obo/GO_1901363 heterocyclic compound binding http://purl.obolibrary.org/obo/GO_0005488 binding http://purl.obolibrary.org/obo/GO_0000737 DNA catabolic process, endonucleolytic http://purl.obolibrary.org/obo/GO_0090305 nucleic acid phosphodiester bond hydrolysis http://purl.obolibrary.org/obo/GO_0098781 ncRNA transcription http://purl.obolibrary.org/obo/GO_0006351 transcription, DNA-templated http://purl.obolibrary.org/obo/GO_0045892 negative regulation of transcription, DNA-templated http://purl.obolibrary.org/obo/GO_1903507 negative regulation of nucleic acid-templated transcription http://purl.obolibrary.org/obo/GO_0140747 regulation of ncRNA transcription http://purl.obolibrary.org/obo/GO_0006355 regulation of transcription, DNA-templated http://purl.obolibrary.org/obo/GO_0015908 fatty acid transport http://purl.obolibrary.org/obo/GO_0015849 organic acid transport http://purl.obolibrary.org/obo/GO_1902742 apoptotic process involved in development http://purl.obolibrary.org/obo/GO_0006915 apoptotic process http://purl.obolibrary.org/obo/GO_0009611 response to wounding http://purl.obolibrary.org/obo/GO_0006950 response to stress http://purl.obolibrary.org/obo/GO_0007000 nucleolus organization http://purl.obolibrary.org/obo/GO_0006997 nucleus organization http://purl.obolibrary.org/obo/GO_0008152 metabolic process http://purl.obolibrary.org/obo/GO_0008150 biological_process http://purl.obolibrary.org/obo/GO_0065007 biological regulation http://purl.obolibrary.org/obo/GO_0008150 biological_process http://purl.obolibrary.org/obo/GO_0032501 multicellular organismal process http://purl.obolibrary.org/obo/GO_0008150 biological_process http://purl.obolibrary.org/obo/GO_0032502 developmental process http://purl.obolibrary.org/obo/GO_0008150 biological_process http://purl.obolibrary.org/obo/GO_0040007 growth http://purl.obolibrary.org/obo/GO_0008150 biological_process http://purl.obolibrary.org/obo/GO_0040011 locomotion http://purl.obolibrary.org/obo/GO_0008150 biological_process http://purl.obolibrary.org/obo/GO_0051179 localization http://purl.obolibrary.org/obo/GO_0008150 biological_process http://purl.obolibrary.org/obo/GO_0044249 cellular biosynthetic process http://purl.obolibrary.org/obo/GO_0044237 cellular metabolic process http://purl.obolibrary.org/obo/GO_0032774 RNA biosynthetic process http://purl.obolibrary.org/obo/GO_0034654 nucleobase-containing compound biosynthetic process http://purl.obolibrary.org/obo/GO_0042060 wound healing http://purl.obolibrary.org/obo/GO_0009611 response to wounding http://purl.obolibrary.org/obo/GO_0006276 plasmid maintenance http://purl.obolibrary.org/obo/GO_0009987 cellular process http://purl.obolibrary.org/obo/GO_0006928 movement of cell or subcellular component http://purl.obolibrary.org/obo/GO_0009987 cellular process http://purl.obolibrary.org/obo/GO_0097194 execution phase of apoptosis http://purl.obolibrary.org/obo/GO_0009987 cellular process http://purl.obolibrary.org/obo/GO_0051641 cellular localization http://purl.obolibrary.org/obo/GO_0051179 localization http://purl.obolibrary.org/obo/GO_0016049 cell growth http://purl.obolibrary.org/obo/GO_0040007 growth http://purl.obolibrary.org/obo/GO_0048869 cellular developmental process http://purl.obolibrary.org/obo/GO_0032502 developmental process http://purl.obolibrary.org/obo/GO_0098743 cell aggregation http://purl.obolibrary.org/obo/GO_0009987 cellular process http://purl.obolibrary.org/obo/GO_0051674 localization of cell http://purl.obolibrary.org/obo/GO_0051179 localization http://purl.obolibrary.org/obo/GO_0008219 cell death http://purl.obolibrary.org/obo/GO_0009987 cellular process http://purl.obolibrary.org/obo/GO_0071840 cellular component organization or biogenesis http://purl.obolibrary.org/obo/GO_0009987 cellular process http://purl.obolibrary.org/obo/GO_0060352 cell adhesion molecule production http://purl.obolibrary.org/obo/GO_0009987 cellular process http://purl.obolibrary.org/obo/GO_0071310 cellular response to organic substance http://purl.obolibrary.org/obo/GO_0070887 cellular response to chemical stimulus http://purl.obolibrary.org/obo/GO_0010243 response to organonitrogen compound http://purl.obolibrary.org/obo/GO_1901698 response to nitrogen compound http://purl.obolibrary.org/obo/GO_0014070 response to organic cyclic compound http://purl.obolibrary.org/obo/GO_0010033 response to organic substance http://purl.obolibrary.org/obo/GO_0001816 cytokine production http://purl.obolibrary.org/obo/GO_0032501 multicellular organismal process http://purl.obolibrary.org/obo/GO_0010608 posttranscriptional regulation of gene expression http://purl.obolibrary.org/obo/GO_0010468 regulation of gene expression http://purl.obolibrary.org/obo/GO_0010629 negative regulation of gene expression http://purl.obolibrary.org/obo/GO_0010605 negative regulation of macromolecule metabolic process http://purl.obolibrary.org/obo/GO_0001817 regulation of cytokine production http://purl.obolibrary.org/obo/GO_0051239 regulation of multicellular organismal process http://purl.obolibrary.org/obo/GO_0006355 regulation of transcription, DNA-templated http://purl.obolibrary.org/obo/GO_1903506 regulation of nucleic acid-templated transcription http://purl.obolibrary.org/obo/GO_0010628 positive regulation of gene expression http://purl.obolibrary.org/obo/GO_0010604 positive regulation of macromolecule metabolic process http://purl.obolibrary.org/obo/GO_0060966 regulation of gene silencing by RNA http://purl.obolibrary.org/obo/GO_0010468 regulation of gene expression http://purl.obolibrary.org/obo/GO_0060147 regulation of posttranscriptional gene silencing http://purl.obolibrary.org/obo/GO_0010468 regulation of gene expression http://purl.obolibrary.org/obo/GO_2000112 regulation of cellular macromolecule biosynthetic process http://purl.obolibrary.org/obo/GO_0031326 regulation of cellular biosynthetic process http://purl.obolibrary.org/obo/GO_0010557 positive regulation of macromolecule biosynthetic process http://purl.obolibrary.org/obo/GO_0009891 positive regulation of biosynthetic process http://purl.obolibrary.org/obo/GO_0010558 negative regulation of macromolecule biosynthetic process http://purl.obolibrary.org/obo/GO_0009890 negative regulation of biosynthetic process http://purl.obolibrary.org/obo/GO_2001141 regulation of RNA biosynthetic process http://purl.obolibrary.org/obo/GO_0051252 regulation of RNA metabolic process http://purl.obolibrary.org/obo/GO_1902680 positive regulation of RNA biosynthetic process http://purl.obolibrary.org/obo/GO_2001141 regulation of RNA biosynthetic process http://purl.obolibrary.org/obo/GO_2000573 positive regulation of DNA biosynthetic process http://purl.obolibrary.org/obo/GO_2000278 regulation of DNA biosynthetic process http://purl.obolibrary.org/obo/GO_1902679 negative regulation of RNA biosynthetic process http://purl.obolibrary.org/obo/GO_0051253 negative regulation of RNA metabolic process http://purl.obolibrary.org/obo/GO_0051254 positive regulation of RNA metabolic process http://purl.obolibrary.org/obo/GO_0045935 positive regulation of nucleobase-containing compound metabolic process http://purl.obolibrary.org/obo/GO_0051054 positive regulation of DNA metabolic process http://purl.obolibrary.org/obo/GO_0045935 positive regulation of nucleobase-containing compound metabolic process http://purl.obolibrary.org/obo/GO_0051247 positive regulation of protein metabolic process http://purl.obolibrary.org/obo/GO_0051246 regulation of protein metabolic process http://purl.obolibrary.org/obo/GO_0051053 negative regulation of DNA metabolic process http://purl.obolibrary.org/obo/GO_0045934 negative regulation of nucleobase-containing compound metabolic process http://purl.obolibrary.org/obo/GO_0051248 negative regulation of protein metabolic process http://purl.obolibrary.org/obo/GO_0051172 negative regulation of nitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0051253 negative regulation of RNA metabolic process http://purl.obolibrary.org/obo/GO_0045934 negative regulation of nucleobase-containing compound metabolic process http://purl.obolibrary.org/obo/GO_0006915 apoptotic process http://purl.obolibrary.org/obo/GO_0012501 programmed cell death http://purl.obolibrary.org/obo/GO_0046942 carboxylic acid transport http://purl.obolibrary.org/obo/GO_0015711 organic anion transport http://purl.obolibrary.org/obo/GO_0015909 long-chain fatty acid transport http://purl.obolibrary.org/obo/GO_0015908 fatty acid transport http://purl.obolibrary.org/obo/GO_0034660 ncRNA metabolic process http://purl.obolibrary.org/obo/GO_0016070 RNA metabolic process http://purl.obolibrary.org/obo/GO_0016788 hydrolase activity, acting on ester bonds http://purl.obolibrary.org/obo/GO_0016787 hydrolase activity http://purl.obolibrary.org/obo/GO_0030031 cell projection assembly http://purl.obolibrary.org/obo/GO_0030030 cell projection organization http://purl.obolibrary.org/obo/GO_0010927 cellular component assembly involved in morphogenesis http://purl.obolibrary.org/obo/GO_0048646 anatomical structure formation involved in morphogenesis http://purl.obolibrary.org/obo/GO_0030335 positive regulation of cell migration http://purl.obolibrary.org/obo/GO_2000147 positive regulation of cell motility http://purl.obolibrary.org/obo/GO_0030336 negative regulation of cell migration http://purl.obolibrary.org/obo/GO_2000146 negative regulation of cell motility http://purl.obolibrary.org/obo/GO_0032268 regulation of cellular protein metabolic process http://purl.obolibrary.org/obo/GO_0051246 regulation of protein metabolic process http://purl.obolibrary.org/obo/GO_0031325 positive regulation of cellular metabolic process http://purl.obolibrary.org/obo/GO_0048522 positive regulation of cellular process http://purl.obolibrary.org/obo/GO_0031326 regulation of cellular biosynthetic process http://purl.obolibrary.org/obo/GO_0009889 regulation of biosynthetic process http://purl.obolibrary.org/obo/GO_0031329 regulation of cellular catabolic process http://purl.obolibrary.org/obo/GO_0009894 regulation of catabolic process http://purl.obolibrary.org/obo/GO_0034248 regulation of cellular amide metabolic process http://purl.obolibrary.org/obo/GO_0051171 regulation of nitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0031327 negative regulation of cellular biosynthetic process http://purl.obolibrary.org/obo/GO_0009890 negative regulation of biosynthetic process http://purl.obolibrary.org/obo/GO_0034249 negative regulation of cellular amide metabolic process http://purl.obolibrary.org/obo/GO_0051172 negative regulation of nitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0031330 negative regulation of cellular catabolic process http://purl.obolibrary.org/obo/GO_0009895 negative regulation of catabolic process http://purl.obolibrary.org/obo/GO_0031328 positive regulation of cellular biosynthetic process http://purl.obolibrary.org/obo/GO_0009891 positive regulation of biosynthetic process http://purl.obolibrary.org/obo/GO_0031331 positive regulation of cellular catabolic process http://purl.obolibrary.org/obo/GO_0009896 positive regulation of catabolic process http://purl.obolibrary.org/obo/GO_0034250 positive regulation of cellular amide metabolic process http://purl.obolibrary.org/obo/GO_0051173 positive regulation of nitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_1903624 regulation of DNA catabolic process http://purl.obolibrary.org/obo/GO_0051052 regulation of DNA metabolic process http://purl.obolibrary.org/obo/GO_1903626 positive regulation of DNA catabolic process http://purl.obolibrary.org/obo/GO_1903624 regulation of DNA catabolic process http://purl.obolibrary.org/obo/GO_0031343 positive regulation of cell killing http://purl.obolibrary.org/obo/GO_0048522 positive regulation of cellular process http://purl.obolibrary.org/obo/GO_0032103 positive regulation of response to external stimulus http://purl.obolibrary.org/obo/GO_0048584 positive regulation of response to stimulus http://purl.obolibrary.org/obo/GO_0050920 regulation of chemotaxis http://purl.obolibrary.org/obo/GO_0040012 regulation of locomotion http://purl.obolibrary.org/obo/GO_0032102 negative regulation of response to external stimulus http://purl.obolibrary.org/obo/GO_0048585 negative regulation of response to stimulus http://purl.obolibrary.org/obo/GO_0050921 positive regulation of chemotaxis http://purl.obolibrary.org/obo/GO_0040017 positive regulation of locomotion http://purl.obolibrary.org/obo/GO_0007568 aging http://purl.obolibrary.org/obo/GO_0032502 developmental process http://purl.obolibrary.org/obo/GO_0009653 anatomical structure morphogenesis http://purl.obolibrary.org/obo/GO_0032502 developmental process http://purl.obolibrary.org/obo/GO_0048532 anatomical structure arrangement http://purl.obolibrary.org/obo/GO_0032502 developmental process http://purl.obolibrary.org/obo/GO_0048589 developmental growth http://purl.obolibrary.org/obo/GO_0040007 growth http://purl.obolibrary.org/obo/GO_0048646 anatomical structure formation involved in morphogenesis http://purl.obolibrary.org/obo/GO_0032502 developmental process http://purl.obolibrary.org/obo/GO_0048856 anatomical structure development http://purl.obolibrary.org/obo/GO_0032502 developmental process http://purl.obolibrary.org/obo/GO_0097659 nucleic acid-templated transcription http://purl.obolibrary.org/obo/GO_0032774 RNA biosynthetic process http://purl.obolibrary.org/obo/GO_1902494 catalytic complex http://purl.obolibrary.org/obo/GO_0032991 protein-containing complex http://purl.obolibrary.org/obo/GO_0033044 regulation of chromosome organization http://purl.obolibrary.org/obo/GO_0033043 regulation of organelle organization http://purl.obolibrary.org/obo/GO_1903353 regulation of nucleus organization http://purl.obolibrary.org/obo/GO_0033043 regulation of organelle organization http://purl.obolibrary.org/obo/GO_0010638 positive regulation of organelle organization http://purl.obolibrary.org/obo/GO_0051130 positive regulation of cellular component organization http://purl.obolibrary.org/obo/GO_0010639 negative regulation of organelle organization http://purl.obolibrary.org/obo/GO_0051129 negative regulation of cellular component organization http://purl.obolibrary.org/obo/GO_0001882 nucleoside binding http://purl.obolibrary.org/obo/GO_1901363 heterocyclic compound binding http://purl.obolibrary.org/obo/GO_0001558 regulation of cell growth http://purl.obolibrary.org/obo/GO_0051128 regulation of cellular component organization http://purl.obolibrary.org/obo/GO_0045926 negative regulation of growth http://purl.obolibrary.org/obo/GO_0048519 negative regulation of biological process http://purl.obolibrary.org/obo/GO_0045927 positive regulation of growth http://purl.obolibrary.org/obo/GO_0048518 positive regulation of biological process http://purl.obolibrary.org/obo/GO_0048638 regulation of developmental growth http://purl.obolibrary.org/obo/GO_0050793 regulation of developmental process http://purl.obolibrary.org/obo/GO_0008284 positive regulation of cell population proliferation http://purl.obolibrary.org/obo/GO_0048522 positive regulation of cellular process http://purl.obolibrary.org/obo/GO_0010033 response to organic substance http://purl.obolibrary.org/obo/GO_0042221 response to chemical http://purl.obolibrary.org/obo/GO_1901700 response to oxygen-containing compound http://purl.obolibrary.org/obo/GO_0042221 response to chemical http://purl.obolibrary.org/obo/GO_0046677 response to antibiotic http://purl.obolibrary.org/obo/GO_0042221 response to chemical http://purl.obolibrary.org/obo/GO_0009636 response to toxic substance http://purl.obolibrary.org/obo/GO_0042221 response to chemical http://purl.obolibrary.org/obo/GO_1901698 response to nitrogen compound http://purl.obolibrary.org/obo/GO_0042221 response to chemical http://purl.obolibrary.org/obo/GO_0006935 chemotaxis http://purl.obolibrary.org/obo/GO_0042330 taxis http://purl.obolibrary.org/obo/GO_0098900 regulation of action potential http://purl.obolibrary.org/obo/GO_0050789 regulation of biological process http://purl.obolibrary.org/obo/GO_0043066 negative regulation of apoptotic process http://purl.obolibrary.org/obo/GO_0043069 negative regulation of programmed cell death http://purl.obolibrary.org/obo/GO_1904748 regulation of apoptotic process involved in development http://purl.obolibrary.org/obo/GO_0050793 regulation of developmental process http://purl.obolibrary.org/obo/GO_0043065 positive regulation of apoptotic process http://purl.obolibrary.org/obo/GO_0043068 positive regulation of programmed cell death http://purl.obolibrary.org/obo/GO_0006518 peptide metabolic process http://purl.obolibrary.org/obo/GO_1901564 organonitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0043604 amide biosynthetic process http://purl.obolibrary.org/obo/GO_0044271 cellular nitrogen compound biosynthetic process http://purl.obolibrary.org/obo/GO_0006725 cellular aromatic compound metabolic process http://purl.obolibrary.org/obo/GO_0044237 cellular metabolic process http://purl.obolibrary.org/obo/GO_0044248 cellular catabolic process http://purl.obolibrary.org/obo/GO_0009056 catabolic process http://purl.obolibrary.org/obo/GO_0046483 heterocycle metabolic process http://purl.obolibrary.org/obo/GO_0044237 cellular metabolic process http://purl.obolibrary.org/obo/GO_0060195 negative regulation of antisense RNA transcription http://purl.obolibrary.org/obo/GO_0060194 regulation of antisense RNA transcription http://purl.obolibrary.org/obo/GO_0060196 positive regulation of antisense RNA transcription http://purl.obolibrary.org/obo/GO_0060194 regulation of antisense RNA transcription http://purl.obolibrary.org/obo/GO_0032309 icosanoid secretion http://purl.obolibrary.org/obo/GO_0071715 icosanoid transport http://purl.obolibrary.org/obo/GO_2000679 positive regulation of transcription regulatory region DNA binding http://purl.obolibrary.org/obo/GO_2000677 regulation of transcription regulatory region DNA binding http://purl.obolibrary.org/obo/GO_0048584 positive regulation of response to stimulus http://purl.obolibrary.org/obo/GO_0048583 regulation of response to stimulus http://purl.obolibrary.org/obo/GO_0051094 positive regulation of developmental process http://purl.obolibrary.org/obo/GO_0050793 regulation of developmental process http://purl.obolibrary.org/obo/GO_0051240 positive regulation of multicellular organismal process http://purl.obolibrary.org/obo/GO_0051239 regulation of multicellular organismal process http://purl.obolibrary.org/obo/GO_0040017 positive regulation of locomotion http://purl.obolibrary.org/obo/GO_0040012 regulation of locomotion http://purl.obolibrary.org/obo/GO_0045760 positive regulation of action potential http://purl.obolibrary.org/obo/GO_0098900 regulation of action potential http://purl.obolibrary.org/obo/GO_1903829 positive regulation of protein localization http://purl.obolibrary.org/obo/GO_0032880 regulation of protein localization http://purl.obolibrary.org/obo/GO_0009893 positive regulation of metabolic process http://purl.obolibrary.org/obo/GO_0019222 regulation of metabolic process http://purl.obolibrary.org/obo/GO_0048522 positive regulation of cellular process http://purl.obolibrary.org/obo/GO_0050794 regulation of cellular process http://purl.obolibrary.org/obo/GO_0051050 positive regulation of transport http://purl.obolibrary.org/obo/GO_0051049 regulation of transport http://purl.obolibrary.org/obo/GO_0060148 positive regulation of posttranscriptional gene silencing http://purl.obolibrary.org/obo/GO_0060147 regulation of posttranscriptional gene silencing http://purl.obolibrary.org/obo/GO_1905954 positive regulation of lipid localization http://purl.obolibrary.org/obo/GO_1905952 regulation of lipid localization http://purl.obolibrary.org/obo/GO_0048523 negative regulation of cellular process http://purl.obolibrary.org/obo/GO_0050794 regulation of cellular process http://purl.obolibrary.org/obo/GO_0048585 negative regulation of response to stimulus http://purl.obolibrary.org/obo/GO_0048583 regulation of response to stimulus http://purl.obolibrary.org/obo/GO_0051093 negative regulation of developmental process http://purl.obolibrary.org/obo/GO_0050793 regulation of developmental process http://purl.obolibrary.org/obo/GO_0051241 negative regulation of multicellular organismal process http://purl.obolibrary.org/obo/GO_0051239 regulation of multicellular organismal process http://purl.obolibrary.org/obo/GO_0040013 negative regulation of locomotion http://purl.obolibrary.org/obo/GO_0040012 regulation of locomotion http://purl.obolibrary.org/obo/GO_0045759 negative regulation of action potential http://purl.obolibrary.org/obo/GO_0098900 regulation of action potential http://purl.obolibrary.org/obo/GO_2000678 negative regulation of transcription regulatory region DNA binding http://purl.obolibrary.org/obo/GO_2000677 regulation of transcription regulatory region DNA binding http://purl.obolibrary.org/obo/GO_0009892 negative regulation of metabolic process http://purl.obolibrary.org/obo/GO_0019222 regulation of metabolic process http://purl.obolibrary.org/obo/GO_0051051 negative regulation of transport http://purl.obolibrary.org/obo/GO_0051049 regulation of transport http://purl.obolibrary.org/obo/GO_1903828 negative regulation of protein localization http://purl.obolibrary.org/obo/GO_0032880 regulation of protein localization http://purl.obolibrary.org/obo/GO_0060149 negative regulation of posttranscriptional gene silencing http://purl.obolibrary.org/obo/GO_0060147 regulation of posttranscriptional gene silencing http://purl.obolibrary.org/obo/GO_0060967 negative regulation of gene silencing by RNA http://purl.obolibrary.org/obo/GO_0060966 regulation of gene silencing by RNA http://purl.obolibrary.org/obo/GO_1905953 negative regulation of lipid localization http://purl.obolibrary.org/obo/GO_1905952 regulation of lipid localization http://purl.obolibrary.org/obo/GO_0002831 regulation of response to biotic stimulus http://purl.obolibrary.org/obo/GO_0048583 regulation of response to stimulus http://purl.obolibrary.org/obo/GO_0032101 regulation of response to external stimulus http://purl.obolibrary.org/obo/GO_0048583 regulation of response to stimulus http://purl.obolibrary.org/obo/GO_0080134 regulation of response to stress http://purl.obolibrary.org/obo/GO_0048583 regulation of response to stimulus http://purl.obolibrary.org/obo/GO_0060560 developmental growth involved in morphogenesis http://purl.obolibrary.org/obo/GO_0048589 developmental growth http://purl.obolibrary.org/obo/GO_0042246 tissue regeneration http://purl.obolibrary.org/obo/GO_0031099 regeneration http://purl.obolibrary.org/obo/GO_0031099 regeneration http://purl.obolibrary.org/obo/GO_0048856 anatomical structure development http://purl.obolibrary.org/obo/GO_0016477 cell migration http://purl.obolibrary.org/obo/GO_0048870 cell motility http://purl.obolibrary.org/obo/GO_0040008 regulation of growth http://purl.obolibrary.org/obo/GO_0050789 regulation of biological process http://purl.obolibrary.org/obo/GO_0048518 positive regulation of biological process http://purl.obolibrary.org/obo/GO_0050789 regulation of biological process http://purl.obolibrary.org/obo/GO_0048519 negative regulation of biological process http://purl.obolibrary.org/obo/GO_0050789 regulation of biological process http://purl.obolibrary.org/obo/GO_0048583 regulation of response to stimulus http://purl.obolibrary.org/obo/GO_0050789 regulation of biological process http://purl.obolibrary.org/obo/GO_0050794 regulation of cellular process http://purl.obolibrary.org/obo/GO_0050789 regulation of biological process http://purl.obolibrary.org/obo/GO_0051239 regulation of multicellular organismal process http://purl.obolibrary.org/obo/GO_0050789 regulation of biological process http://purl.obolibrary.org/obo/GO_0050793 regulation of developmental process http://purl.obolibrary.org/obo/GO_0050789 regulation of biological process http://purl.obolibrary.org/obo/GO_0040012 regulation of locomotion http://purl.obolibrary.org/obo/GO_0050789 regulation of biological process http://purl.obolibrary.org/obo/GO_2000677 regulation of transcription regulatory region DNA binding http://purl.obolibrary.org/obo/GO_0051101 regulation of DNA binding http://purl.obolibrary.org/obo/GO_0019222 regulation of metabolic process http://purl.obolibrary.org/obo/GO_0050789 regulation of biological process http://purl.obolibrary.org/obo/GO_0032879 regulation of localization http://purl.obolibrary.org/obo/GO_0050789 regulation of biological process http://purl.obolibrary.org/obo/GO_0044087 regulation of cellular component biogenesis http://purl.obolibrary.org/obo/GO_0050794 regulation of cellular process http://purl.obolibrary.org/obo/GO_0051270 regulation of cellular component movement http://purl.obolibrary.org/obo/GO_0032879 regulation of localization http://purl.obolibrary.org/obo/GO_0031323 regulation of cellular metabolic process http://purl.obolibrary.org/obo/GO_0019222 regulation of metabolic process http://purl.obolibrary.org/obo/GO_0031341 regulation of cell killing http://purl.obolibrary.org/obo/GO_0050794 regulation of cellular process http://purl.obolibrary.org/obo/GO_0042127 regulation of cell population proliferation http://purl.obolibrary.org/obo/GO_0050794 regulation of cellular process http://purl.obolibrary.org/obo/GO_0051128 regulation of cellular component organization http://purl.obolibrary.org/obo/GO_0050794 regulation of cellular process http://purl.obolibrary.org/obo/GO_0080135 regulation of cellular response to stress http://purl.obolibrary.org/obo/GO_0080134 regulation of response to stress http://purl.obolibrary.org/obo/GO_0034762 regulation of transmembrane transport http://purl.obolibrary.org/obo/GO_0051049 regulation of transport http://purl.obolibrary.org/obo/GO_0090342 regulation of cell aging http://purl.obolibrary.org/obo/GO_0050793 regulation of developmental process http://purl.obolibrary.org/obo/GO_1900117 regulation of execution phase of apoptosis http://purl.obolibrary.org/obo/GO_0050794 regulation of cellular process http://purl.obolibrary.org/obo/GO_0010941 regulation of cell death http://purl.obolibrary.org/obo/GO_0050794 regulation of cellular process http://purl.obolibrary.org/obo/GO_0060341 regulation of cellular localization http://purl.obolibrary.org/obo/GO_0032879 regulation of localization http://purl.obolibrary.org/obo/GO_0060353 regulation of cell adhesion molecule production http://purl.obolibrary.org/obo/GO_0050794 regulation of cellular process http://purl.obolibrary.org/obo/GO_0050922 negative regulation of chemotaxis http://purl.obolibrary.org/obo/GO_0032102 negative regulation of response to external stimulus http://purl.obolibrary.org/obo/GO_0051047 positive regulation of secretion http://purl.obolibrary.org/obo/GO_0051050 positive regulation of transport http://purl.obolibrary.org/obo/GO_0051048 negative regulation of secretion http://purl.obolibrary.org/obo/GO_0051051 negative regulation of transport http://purl.obolibrary.org/obo/GO_0032305 positive regulation of icosanoid secretion http://purl.obolibrary.org/obo/GO_2000193 positive regulation of fatty acid transport http://purl.obolibrary.org/obo/GO_0032304 negative regulation of icosanoid secretion http://purl.obolibrary.org/obo/GO_2000192 negative regulation of fatty acid transport http://purl.obolibrary.org/obo/GO_0032070 regulation of deoxyribonuclease activity http://purl.obolibrary.org/obo/GO_0032069 regulation of nuclease activity http://purl.obolibrary.org/obo/GO_0048640 negative regulation of developmental growth http://purl.obolibrary.org/obo/GO_0048638 regulation of developmental growth http://purl.obolibrary.org/obo/GO_1904747 positive regulation of apoptotic process involved in development http://purl.obolibrary.org/obo/GO_0043065 positive regulation of apoptotic process http://purl.obolibrary.org/obo/GO_0048639 positive regulation of developmental growth http://purl.obolibrary.org/obo/GO_0048638 regulation of developmental growth http://purl.obolibrary.org/obo/GO_0090343 positive regulation of cell aging http://purl.obolibrary.org/obo/GO_0048522 positive regulation of cellular process http://purl.obolibrary.org/obo/GO_0033043 regulation of organelle organization http://purl.obolibrary.org/obo/GO_0051128 regulation of cellular component organization http://purl.obolibrary.org/obo/GO_0031344 regulation of cell projection organization http://purl.obolibrary.org/obo/GO_0051128 regulation of cellular component organization http://purl.obolibrary.org/obo/GO_0051130 positive regulation of cellular component organization http://purl.obolibrary.org/obo/GO_0048522 positive regulation of cellular process http://purl.obolibrary.org/obo/GO_2000146 negative regulation of cell motility http://purl.obolibrary.org/obo/GO_0040013 negative regulation of locomotion http://purl.obolibrary.org/obo/GO_2000147 positive regulation of cell motility http://purl.obolibrary.org/obo/GO_0040017 positive regulation of locomotion http://purl.obolibrary.org/obo/GO_0010468 regulation of gene expression http://purl.obolibrary.org/obo/GO_0060255 regulation of macromolecule metabolic process http://purl.obolibrary.org/obo/GO_0010556 regulation of macromolecule biosynthetic process http://purl.obolibrary.org/obo/GO_0009889 regulation of biosynthetic process http://purl.obolibrary.org/obo/GO_0010604 positive regulation of macromolecule metabolic process http://purl.obolibrary.org/obo/GO_0009893 positive regulation of metabolic process http://purl.obolibrary.org/obo/GO_0010605 negative regulation of macromolecule metabolic process http://purl.obolibrary.org/obo/GO_0009892 negative regulation of metabolic process http://purl.obolibrary.org/obo/GO_0032069 regulation of nuclease activity http://purl.obolibrary.org/obo/GO_0051336 regulation of hydrolase activity http://purl.obolibrary.org/obo/GO_0042391 regulation of membrane potential http://purl.obolibrary.org/obo/GO_0065008 regulation of biological quality http://purl.obolibrary.org/obo/GO_1901699 cellular response to nitrogen compound http://purl.obolibrary.org/obo/GO_1901698 response to nitrogen compound http://purl.obolibrary.org/obo/GO_1901701 cellular response to oxygen-containing compound http://purl.obolibrary.org/obo/GO_1901700 response to oxygen-containing compound http://purl.obolibrary.org/obo/GO_0090592 DNA synthesis involved in DNA replication http://purl.obolibrary.org/obo/GO_0071897 DNA biosynthetic process http://purl.obolibrary.org/obo/GO_2001020 regulation of response to DNA damage stimulus http://purl.obolibrary.org/obo/GO_0080135 regulation of cellular response to stress http://purl.obolibrary.org/obo/GO_0003676 nucleic acid binding http://purl.obolibrary.org/obo/GO_1901363 heterocyclic compound binding http://purl.obolibrary.org/obo/GO_0006351 transcription, DNA-templated http://purl.obolibrary.org/obo/GO_0097659 nucleic acid-templated transcription http://purl.obolibrary.org/obo/GO_0042710 biofilm formation http://purl.obolibrary.org/obo/GO_0098630 aggregation of unicellular organisms http://purl.obolibrary.org/obo/GO_0098630 aggregation of unicellular organisms http://purl.obolibrary.org/obo/GO_0098743 cell aggregation http://purl.obolibrary.org/obo/GO_1901328 response to cytochalasin B http://purl.obolibrary.org/obo/GO_0014070 response to organic cyclic compound http://purl.obolibrary.org/obo/GO_1990234 transferase complex http://purl.obolibrary.org/obo/GO_1902494 catalytic complex http://purl.obolibrary.org/obo/GO_0060561 apoptotic process involved in morphogenesis http://purl.obolibrary.org/obo/GO_1902742 apoptotic process involved in development http://purl.obolibrary.org/obo/GO_1903507 negative regulation of nucleic acid-templated transcription http://purl.obolibrary.org/obo/GO_1902679 negative regulation of RNA biosynthetic process http://purl.obolibrary.org/obo/GO_1902338 negative regulation of apoptotic process involved in morphogenesis http://purl.obolibrary.org/obo/GO_1902337 regulation of apoptotic process involved in morphogenesis http://purl.obolibrary.org/obo/GO_1902339 positive regulation of apoptotic process involved in morphogenesis http://purl.obolibrary.org/obo/GO_1902337 regulation of apoptotic process involved in morphogenesis http://purl.obolibrary.org/obo/GO_1902337 regulation of apoptotic process involved in morphogenesis http://purl.obolibrary.org/obo/GO_1904748 regulation of apoptotic process involved in development http://purl.obolibrary.org/obo/GO_0034708 methyltransferase complex http://purl.obolibrary.org/obo/GO_1990234 transferase complex http://purl.obolibrary.org/obo/GO_1903506 regulation of nucleic acid-templated transcription http://purl.obolibrary.org/obo/GO_2001141 regulation of RNA biosynthetic process http://purl.obolibrary.org/obo/GO_0120036 plasma membrane bounded cell projection organization http://purl.obolibrary.org/obo/GO_0030030 cell projection organization http://purl.obolibrary.org/obo/GO_0035194 post-transcriptional gene silencing by RNA http://purl.obolibrary.org/obo/GO_0016441 posttranscriptional gene silencing http://purl.obolibrary.org/obo/GO_0032075 positive regulation of nuclease activity http://purl.obolibrary.org/obo/GO_0051345 positive regulation of hydrolase activity http://purl.obolibrary.org/obo/GO_0032074 negative regulation of nuclease activity http://purl.obolibrary.org/obo/GO_0051346 negative regulation of hydrolase activity http://purl.obolibrary.org/obo/GO_0032071 regulation of endodeoxyribonuclease activity http://purl.obolibrary.org/obo/GO_0032070 regulation of deoxyribonuclease activity http://purl.obolibrary.org/obo/GO_0032078 negative regulation of endodeoxyribonuclease activity http://purl.obolibrary.org/obo/GO_0032076 negative regulation of deoxyribonuclease activity http://purl.obolibrary.org/obo/GO_0032079 positive regulation of endodeoxyribonuclease activity http://purl.obolibrary.org/obo/GO_0032077 positive regulation of deoxyribonuclease activity http://purl.obolibrary.org/obo/GO_0090237 regulation of arachidonic acid secretion http://purl.obolibrary.org/obo/GO_0032303 regulation of icosanoid secretion http://purl.obolibrary.org/obo/GO_1900139 negative regulation of arachidonic acid secretion http://purl.obolibrary.org/obo/GO_0090237 regulation of arachidonic acid secretion http://purl.obolibrary.org/obo/GO_0090238 positive regulation of arachidonic acid secretion http://purl.obolibrary.org/obo/GO_0090237 regulation of arachidonic acid secretion http://purl.obolibrary.org/obo/GO_2000192 negative regulation of fatty acid transport http://purl.obolibrary.org/obo/GO_2000191 regulation of fatty acid transport http://purl.obolibrary.org/obo/GO_2000193 positive regulation of fatty acid transport http://purl.obolibrary.org/obo/GO_2000191 regulation of fatty acid transport http://purl.obolibrary.org/obo/GO_0022898 regulation of transmembrane transporter activity http://purl.obolibrary.org/obo/GO_0034762 regulation of transmembrane transport http://purl.obolibrary.org/obo/GO_0032410 negative regulation of transporter activity http://purl.obolibrary.org/obo/GO_0051051 negative regulation of transport http://purl.obolibrary.org/obo/GO_0032411 positive regulation of transporter activity http://purl.obolibrary.org/obo/GO_0051050 positive regulation of transport http://purl.obolibrary.org/obo/GO_0032413 negative regulation of ion transmembrane transporter activity http://purl.obolibrary.org/obo/GO_0032412 regulation of ion transmembrane transporter activity http://purl.obolibrary.org/obo/GO_0032414 positive regulation of ion transmembrane transporter activity http://purl.obolibrary.org/obo/GO_0032412 regulation of ion transmembrane transporter activity http://purl.obolibrary.org/obo/GO_0032720 negative regulation of tumor necrosis factor production http://purl.obolibrary.org/obo/GO_1903556 negative regulation of tumor necrosis factor superfamily cytokine production http://purl.obolibrary.org/obo/GO_0032760 positive regulation of tumor necrosis factor production http://purl.obolibrary.org/obo/GO_1903557 positive regulation of tumor necrosis factor superfamily cytokine production http://purl.obolibrary.org/obo/GO_0070201 regulation of establishment of protein localization http://purl.obolibrary.org/obo/GO_0032880 regulation of protein localization http://purl.obolibrary.org/obo/GO_0032891 negative regulation of organic acid transport http://purl.obolibrary.org/obo/GO_0051051 negative regulation of transport http://purl.obolibrary.org/obo/GO_0032892 positive regulation of organic acid transport http://purl.obolibrary.org/obo/GO_0051050 positive regulation of transport http://purl.obolibrary.org/obo/GO_2001251 negative regulation of chromosome organization http://purl.obolibrary.org/obo/GO_0010639 negative regulation of organelle organization http://purl.obolibrary.org/obo/GO_2001252 positive regulation of chromosome organization http://purl.obolibrary.org/obo/GO_0010638 positive regulation of organelle organization http://purl.obolibrary.org/obo/GO_0034765 regulation of ion transmembrane transport http://purl.obolibrary.org/obo/GO_0043269 regulation of ion transport http://purl.obolibrary.org/obo/GO_0034764 positive regulation of transmembrane transport http://purl.obolibrary.org/obo/GO_0051050 positive regulation of transport http://purl.obolibrary.org/obo/GO_0042330 taxis http://purl.obolibrary.org/obo/GO_0040011 locomotion http://purl.obolibrary.org/obo/GO_0032091 negative regulation of protein binding http://purl.obolibrary.org/obo/GO_0051100 negative regulation of binding http://purl.obolibrary.org/obo/GO_0032092 positive regulation of protein binding http://purl.obolibrary.org/obo/GO_0051099 positive regulation of binding http://purl.obolibrary.org/obo/GO_0032056 positive regulation of translation in response to stress http://purl.obolibrary.org/obo/GO_0045727 positive regulation of translation http://purl.obolibrary.org/obo/GO_0043086 negative regulation of catalytic activity http://purl.obolibrary.org/obo/GO_0050790 regulation of catalytic activity http://purl.obolibrary.org/obo/GO_0051100 negative regulation of binding http://purl.obolibrary.org/obo/GO_0051098 regulation of binding http://purl.obolibrary.org/obo/GO_0043085 positive regulation of catalytic activity http://purl.obolibrary.org/obo/GO_0050790 regulation of catalytic activity http://purl.obolibrary.org/obo/GO_0051099 positive regulation of binding http://purl.obolibrary.org/obo/GO_0051098 regulation of binding http://purl.obolibrary.org/obo/GO_0022603 regulation of anatomical structure morphogenesis http://purl.obolibrary.org/obo/GO_0050793 regulation of developmental process http://purl.obolibrary.org/obo/GO_1905643 positive regulation of DNA methylation http://purl.obolibrary.org/obo/GO_0044030 regulation of DNA methylation http://purl.obolibrary.org/obo/GO_0043388 positive regulation of DNA binding http://purl.obolibrary.org/obo/GO_0051101 regulation of DNA binding http://purl.obolibrary.org/obo/GO_1900132 positive regulation of lipid binding http://purl.obolibrary.org/obo/GO_1900130 regulation of lipid binding http://purl.obolibrary.org/obo/GO_0043392 negative regulation of DNA binding http://purl.obolibrary.org/obo/GO_0051101 regulation of DNA binding http://purl.obolibrary.org/obo/GO_1900131 negative regulation of lipid binding http://purl.obolibrary.org/obo/GO_1900130 regulation of lipid binding http://purl.obolibrary.org/obo/GO_0051234 establishment of localization http://purl.obolibrary.org/obo/GO_0051179 localization http://purl.obolibrary.org/obo/GO_0071407 cellular response to organic cyclic compound http://purl.obolibrary.org/obo/GO_0014070 response to organic cyclic compound http://purl.obolibrary.org/obo/GO_0071417 cellular response to organonitrogen compound http://purl.obolibrary.org/obo/GO_0010243 response to organonitrogen compound http://purl.obolibrary.org/obo/GO_0032640 tumor necrosis factor production http://purl.obolibrary.org/obo/GO_0071706 tumor necrosis factor superfamily cytokine production http://purl.obolibrary.org/obo/GO_0032680 regulation of tumor necrosis factor production http://purl.obolibrary.org/obo/GO_1903555 regulation of tumor necrosis factor superfamily cytokine production http://purl.obolibrary.org/obo/GO_0030334 regulation of cell migration http://purl.obolibrary.org/obo/GO_2000145 regulation of cell motility http://purl.obolibrary.org/obo/GO_0070549 siRNA-mediated gene silencing by inhibition of translation http://purl.obolibrary.org/obo/GO_0040033 RNA-mediated gene silencing by inhibition of translation http://purl.obolibrary.org/obo/GO_0043393 regulation of protein binding http://purl.obolibrary.org/obo/GO_0051098 regulation of binding http://purl.obolibrary.org/obo/GO_0051101 regulation of DNA binding http://purl.obolibrary.org/obo/GO_0051098 regulation of binding http://purl.obolibrary.org/obo/GO_1900130 regulation of lipid binding http://purl.obolibrary.org/obo/GO_0051098 regulation of binding http://purl.obolibrary.org/obo/GO_0043069 negative regulation of programmed cell death http://purl.obolibrary.org/obo/GO_0043067 regulation of programmed cell death http://purl.obolibrary.org/obo/GO_0072749 cellular response to cytochalasin B http://purl.obolibrary.org/obo/GO_1901328 response to cytochalasin B http://purl.obolibrary.org/obo/GO_1900119 positive regulation of execution phase of apoptosis http://purl.obolibrary.org/obo/GO_0043065 positive regulation of apoptotic process http://purl.obolibrary.org/obo/GO_1902511 negative regulation of apoptotic DNA fragmentation http://purl.obolibrary.org/obo/GO_1903625 negative regulation of DNA catabolic process http://purl.obolibrary.org/obo/GO_1902512 positive regulation of apoptotic DNA fragmentation http://purl.obolibrary.org/obo/GO_1903626 positive regulation of DNA catabolic process http://purl.obolibrary.org/obo/GO_1902510 regulation of apoptotic DNA fragmentation http://purl.obolibrary.org/obo/GO_1903624 regulation of DNA catabolic process http://purl.obolibrary.org/obo/GO_1904796 regulation of core promoter binding http://purl.obolibrary.org/obo/GO_2000677 regulation of transcription regulatory region DNA binding http://purl.obolibrary.org/obo/GO_0005622 intracellular anatomical structure http://purl.obolibrary.org/obo/GO_0110165 cellular anatomical entity http://purl.obolibrary.org/obo/GO_0012501 programmed cell death http://purl.obolibrary.org/obo/GO_0008219 cell death http://purl.obolibrary.org/obo/GO_0071495 cellular response to endogenous stimulus http://purl.obolibrary.org/obo/GO_0009719 response to endogenous stimulus http://purl.obolibrary.org/obo/GO_0009890 negative regulation of biosynthetic process http://purl.obolibrary.org/obo/GO_0009892 negative regulation of metabolic process http://purl.obolibrary.org/obo/GO_0009891 positive regulation of biosynthetic process http://purl.obolibrary.org/obo/GO_0009893 positive regulation of metabolic process http://purl.obolibrary.org/obo/GO_0009895 negative regulation of catabolic process http://purl.obolibrary.org/obo/GO_0009894 regulation of catabolic process http://purl.obolibrary.org/obo/GO_0051172 negative regulation of nitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0051171 regulation of nitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0051173 positive regulation of nitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0051171 regulation of nitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0009896 positive regulation of catabolic process http://purl.obolibrary.org/obo/GO_0009894 regulation of catabolic process http://purl.obolibrary.org/obo/GO_0010942 positive regulation of cell death http://purl.obolibrary.org/obo/GO_0048522 positive regulation of cellular process http://purl.obolibrary.org/obo/GO_0043067 regulation of programmed cell death http://purl.obolibrary.org/obo/GO_0010941 regulation of cell death http://purl.obolibrary.org/obo/GO_0043068 positive regulation of programmed cell death http://purl.obolibrary.org/obo/GO_0043067 regulation of programmed cell death http://purl.obolibrary.org/obo/GO_0080090 regulation of primary metabolic process http://purl.obolibrary.org/obo/GO_0019222 regulation of metabolic process http://purl.obolibrary.org/obo/GO_0060255 regulation of macromolecule metabolic process http://purl.obolibrary.org/obo/GO_0019222 regulation of metabolic process http://purl.obolibrary.org/obo/GO_0009889 regulation of biosynthetic process http://purl.obolibrary.org/obo/GO_0019222 regulation of metabolic process http://purl.obolibrary.org/obo/GO_0009894 regulation of catabolic process http://purl.obolibrary.org/obo/GO_0019222 regulation of metabolic process http://purl.obolibrary.org/obo/GO_0051171 regulation of nitrogen compound metabolic process http://purl.obolibrary.org/obo/GO_0019222 regulation of metabolic process http://purl.obolibrary.org/obo/GO_0015267 channel activity http://purl.obolibrary.org/obo/GO_0022803 passive transmembrane transporter activity http://purl.obolibrary.org/obo/GO_0031345 negative regulation of cell projection organization http://purl.obolibrary.org/obo/GO_0051129 negative regulation of cellular component organization http://purl.obolibrary.org/obo/GO_0031346 positive regulation of cell projection organization http://purl.obolibrary.org/obo/GO_0051130 positive regulation of cellular component organization http://purl.obolibrary.org/obo/GO_0120035 regulation of plasma membrane bounded cell projection organization http://purl.obolibrary.org/obo/GO_0031344 regulation of cell projection organization http://purl.obolibrary.org/obo/GO_0120034 positive regulation of plasma membrane bounded cell projection assembly http://purl.obolibrary.org/obo/GO_0120032 regulation of plasma membrane bounded cell projection assembly http://purl.obolibrary.org/obo/GO_0032880 regulation of protein localization http://purl.obolibrary.org/obo/GO_0032879 regulation of localization http://purl.obolibrary.org/obo/GO_0051049 regulation of transport http://purl.obolibrary.org/obo/GO_0032879 regulation of localization http://purl.obolibrary.org/obo/GO_1905952 regulation of lipid localization http://purl.obolibrary.org/obo/GO_0032879 regulation of localization http://purl.obolibrary.org/obo/GO_0009300 antisense RNA transcription http://purl.obolibrary.org/obo/GO_0098781 ncRNA transcription http://purl.obolibrary.org/obo/GO_0042981 regulation of apoptotic process http://purl.obolibrary.org/obo/GO_0043067 regulation of programmed cell death http://purl.obolibrary.org/obo/GO_0044070 regulation of anion transport http://purl.obolibrary.org/obo/GO_0043269 regulation of ion transport http://purl.obolibrary.org/obo/GO_0043270 positive regulation of ion transport http://purl.obolibrary.org/obo/GO_0051050 positive regulation of transport http://purl.obolibrary.org/obo/GO_0043271 negative regulation of ion transport http://purl.obolibrary.org/obo/GO_0051051 negative regulation of transport http://purl.obolibrary.org/obo/GO_0060355 positive regulation of cell adhesion molecule production http://purl.obolibrary.org/obo/GO_0060353 regulation of cell adhesion molecule production http://purl.obolibrary.org/obo/GO_0051336 regulation of hydrolase activity http://purl.obolibrary.org/obo/GO_0050790 regulation of catalytic activity http://purl.obolibrary.org/obo/GO_0032368 regulation of lipid transport http://purl.obolibrary.org/obo/GO_1905952 regulation of lipid localization http://purl.obolibrary.org/obo/GO_0051046 regulation of secretion http://purl.obolibrary.org/obo/GO_0051049 regulation of transport http://purl.obolibrary.org/obo/GO_0032890 regulation of organic acid transport http://purl.obolibrary.org/obo/GO_0051049 regulation of transport http://purl.obolibrary.org/obo/GO_0043269 regulation of ion transport http://purl.obolibrary.org/obo/GO_0051049 regulation of transport http://purl.obolibrary.org/obo/GO_0120032 regulation of plasma membrane bounded cell projection assembly http://purl.obolibrary.org/obo/GO_0120035 regulation of plasma membrane bounded cell projection organization http://purl.obolibrary.org/obo/GO_0032409 regulation of transporter activity http://purl.obolibrary.org/obo/GO_0065009 regulation of molecular function http://purl.obolibrary.org/obo/GO_0044092 negative regulation of molecular function http://purl.obolibrary.org/obo/GO_0065009 regulation of molecular function http://purl.obolibrary.org/obo/GO_0044093 positive regulation of molecular function http://purl.obolibrary.org/obo/GO_0065009 regulation of molecular function http://purl.obolibrary.org/obo/GO_0051098 regulation of binding http://purl.obolibrary.org/obo/GO_0065009 regulation of molecular function http://purl.obolibrary.org/obo/GO_0050790 regulation of catalytic activity http://purl.obolibrary.org/obo/GO_0065009 regulation of molecular function http://purl.obolibrary.org/obo/GO_0016043 cellular component organization http://purl.obolibrary.org/obo/GO_0071840 cellular component organization or biogenesis http://purl.obolibrary.org/obo/GO_0044085 cellular component biogenesis http://purl.obolibrary.org/obo/GO_0071840 cellular component organization or biogenesis http://purl.obolibrary.org/obo/GO_1903034 regulation of response to wounding http://purl.obolibrary.org/obo/GO_0080134 regulation of response to stress http://purl.obolibrary.org/obo/GO_0140097 catalytic activity, acting on DNA http://purl.obolibrary.org/obo/GO_0140640 catalytic activity, acting on a nucleic acid http://purl.obolibrary.org/obo/GO_0060194 regulation of antisense RNA transcription http://purl.obolibrary.org/obo/GO_0140747 regulation of ncRNA transcription http://purl.obolibrary.org/obo/GO_0032060 bleb assembly http://purl.obolibrary.org/obo/GO_0120031 plasma membrane bounded cell projection assembly http://purl.obolibrary.org/obo/GO_1904170 regulation of bleb assembly http://purl.obolibrary.org/obo/GO_0120032 regulation of plasma membrane bounded cell projection assembly http://purl.obolibrary.org/obo/ECO_0007705 obsolete protein assay evidence used in manual assertion OBSOLETE. A type of protein assay evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007784 obsolete protein assay evidence used in automatic assertion OBSOLETE. A type of protein assay evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/OBI_0000973 sequence data A measurement datum that representing the primary structure of a macromolecule(it's sequence) sometimes associated with an indicator of confidence of that measurement. http://purl.obolibrary.org/obo/OBI_0001503 MHC protein complex binding to ligand The process in which a ligand binds to an MHC molecule to form a stable complex http://purl.obolibrary.org/obo/ECO_0000000 evidence A type of information that is used to support an assertion. http://purl.obolibrary.org/obo/ECO_0000026 obsolete nucleic acid hybridization evidence OBSOLETE. A type of experimental genomic evidence resulting from a process in which single stranded nucleic acids are allowed to interact so that complexes, or hybrids, are formed by molecules with sufficiently similar, complementary sequences. http://purl.obolibrary.org/obo/ECO_0000039 obsolete protein assay evidence OBSOLETE. A type of direct assay evidence resulting from determining the presence, abundance, structure, function, or activity of proteins. http://purl.obolibrary.org/obo/ECO_0000073 obsolete experimental genomic evidence OBSOLETE. A type of experimental evidence that is based on the characterization of an attribute of the genome underlying a gene product. http://purl.obolibrary.org/obo/ECO_0000082 obsolete polymerase chain reaction evidence OBSOLETE. A type of experimental genomic evidence where DNA polymerase is used to synthesize new strand of DNA complementary to the offered template strand. http://purl.obolibrary.org/obo/ECO_0000098 obsolete in situ hybridization evidence OBSOLETE. A type of nucleic acid hybridization evidence based on localization of a specific segment of DNA or RNA by the application of a complementary strand of nucleic acid to which a reporter molecule is attached. http://purl.obolibrary.org/obo/ECO_0000217 assertion method A means by which a statement is made about an entity. http://purl.obolibrary.org/obo/ECO_0000236 obsolete chromosome conformation capture-PCR evidence OBSOLETE. A type of chromosome conformation capture evidence that is derived by performing polymerase chain reaction (PCR) in the quantitation step. http://purl.obolibrary.org/obo/ECO_0000246 obsolete computational combinatorial evidence used in automatic assertion OBSOLETE. A type of computational combinatorial analysis that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0001056 obsolete induced mutation evidence OBSOLETE. A type of genetic transformation evidence resulting from a mutation induced by a mutagenic compounds or irradiation. http://purl.obolibrary.org/obo/ECO_0001223 obsolete induced mutation evidence used in manual assertion OBSOLETE. A type of induced mutation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0005611 obsolete inference from experimental data evidence OBSOLETE. A type of direct assay evidence used in manual assertion where phenotype or disease association is made with the gene because of involvement in the molecular mechanism of disease or involvement in a pathway known to contribute to a disease. http://purl.obolibrary.org/obo/ECO_0005612 obsolete inference from phenotype manipulation evidence OBSOLETE. A type of mutant phenotype evidence used in manual assertion where variations or changes, such as mutations or abnormal levels of the product(s) of a single gene of interest, including non-mutational changes such as inhibition with antibodies or other inhibitors are determined. http://purl.obolibrary.org/obo/ECO_0007158 obsolete visual sequence inspection evidence used in manual assertion OBSOLETE. A type of visual sequence inspection evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007189 obsolete inference from experimental data evidence used in manual assertion OBSOLETE. A type of inference from experimental data evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007190 obsolete inference from phenotype manipulation evidence used in manual assertion OBSOLETE. A type of inference from phenotype manipulation evidence that is used in a manual assertion. http://purl.obolibrary.org/obo/ECO_0007262 obsolete visual sequence inspection evidence used in automatic assertion OBSOLETE. A type of visual sequence inspection evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007293 obsolete inference from experimental data evidence used in automatic assertion OBSOLETE. A type of inference from experimental data evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007294 obsolete inference from phenotype manipulation evidence used in automatic assertion OBSOLETE. A type of inference from phenotype manipulation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/ECO_0007426 obsolete induced mutation evidence used in automatic assertion OBSOLETE. A type of induced mutation evidence that is used in an automatic assertion. http://purl.obolibrary.org/obo/BFO_0000001 entity http://purl.obolibrary.org/obo/ECO_0006065 obsolete in vitro cell based assay evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0000037 obsolete not_recorded http://purl.obolibrary.org/obo/ECO_0000055 obsolete array experiment evidence http://purl.obolibrary.org/obo/ECO_0000072 obsolete Sos-recruitment assay evidence http://purl.obolibrary.org/obo/ECO_0000094 obsolete biological assay evidence http://purl.obolibrary.org/obo/ECO_0000148 obsolete in vitro binding evidence http://purl.obolibrary.org/obo/ECO_0000182 obsolete in vitro culture assay evidence http://purl.obolibrary.org/obo/ECO_0000271 obsolete array experiment evidence used in manual assertion http://purl.obolibrary.org/obo/ECO_0005014 obsolete in vitro cell based assay evidence http://purl.obolibrary.org/obo/ECO_0005538 obsolete computationally derived logical inference http://purl.obolibrary.org/obo/ECO_0005539 obsolete computationally derived logical inference used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005540 obsolete computationally derived logical inference from automatic assertion used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005541 obsolete computationally derived logical inference from manual assertion used in automatic assertion http://purl.obolibrary.org/obo/ECO_0005565 obsolete single channel conductance evidence http://purl.obolibrary.org/obo/ECO_0005567 obsolete steady state activation curve evidence http://purl.obolibrary.org/obo/ECO_0005568 obsolete steady state inactivation curve evidence http://purl.obolibrary.org/obo/ECO_0005569 obsolete window current trace evidence http://purl.obolibrary.org/obo/IAO_0000221 is quality measurement of http://purl.obolibrary.org/obo/IAO_0000136 is about m is a quality measurement of q at t. When q is a quality, there is a measurement process p that has specified output m, a measurement datum, that is about q http://purl.obolibrary.org/obo/OBI_0000293 has_specified_input http://purl.obolibrary.org/obo/RO_0000057 has participant The inverse property of is_specified_input_of http://purl.obolibrary.org/obo/OBI_0000295 is_specified_input_of http://purl.obolibrary.org/obo/RO_0000056 participates in A relation between a planned process and a continuant participating in that process that is not created during the process. The presence of the continuant during the process is explicitly specified in the plan specification which the process realizes the concretization of. http://purl.obolibrary.org/obo/OBI_0000299 has_specified_output http://purl.obolibrary.org/obo/RO_0000057 has participant The inverse property of is_specified_output_of http://purl.obolibrary.org/obo/OBI_0000312 is_specified_output_of http://purl.obolibrary.org/obo/RO_0000056 participates in A relation between a planned process and a continuant participating in that process. The presence of the continuant at the end of the process is explicitly specified in the objective specification which the process realizes the concretization of. http://purl.obolibrary.org/obo/OBI_0000643 has grain http://purl.obolibrary.org/obo/BFO_0000051 has part the relation of the cells in the finger of the skin to the finger, in which an indeterminate number of grains are parts of the whole by virtue of being grains in a collective that is part of the whole, and in which removing one granular part does not nec- essarily damage or diminish the whole. Ontological Whether there is a fixed, or nearly fixed number of parts - e.g. fingers of the hand, chambers of the heart, or wheels of a car - such that there can be a notion of a single one being missing, or whether, by contrast, the number of parts is indeterminate - e.g., cells in the skin of the hand, red cells in blood, or rubber molecules in the tread of the tire of the wheel of the car. http://purl.obolibrary.org/obo/OBI_0001937 has specified numeric value http://purl.obolibrary.org/obo/OBI_0002135 has specified value A relation between a value specification and a number that quantifies it. http://purl.obolibrary.org/obo/OBI_0001938 has value specification http://purl.obolibrary.org/obo/BFO_0000051 has part A relation between an information content entity and a value specification that specifies its value. http://purl.obolibrary.org/obo/OBI_1110060 process is result of http://purl.obolibrary.org/obo/RO_0002087 immediately preceded by is a relationship between a process and a preceding occurrent that directly caused the later one to occur http://purl.obolibrary.org/obo/RO_0000079 function of http://purl.obolibrary.org/obo/RO_0000052 inheres in a relation between a function and an independent continuant (the bearer), in which the function specifically depends on the bearer for its existence http://purl.obolibrary.org/obo/RO_0000080 quality of http://purl.obolibrary.org/obo/RO_0000052 inheres in a relation between a quality and an independent continuant (the bearer), in which the quality specifically depends on the bearer for its existence http://purl.obolibrary.org/obo/RO_0000085 has function http://purl.obolibrary.org/obo/RO_0000053 bearer of a relation between an independent continuant (the bearer) and a function, in which the function specifically depends on the bearer for its existence http://purl.obolibrary.org/obo/RO_0000086 has quality http://purl.obolibrary.org/obo/RO_0000053 bearer of a relation between an independent continuant (the bearer) and a quality, in which the quality specifically depends on the bearer for its existence http://purl.obolibrary.org/obo/RO_0002350 member of http://purl.obolibrary.org/obo/BFO_0000050 part of is member of is a mereological relation between a item and a collection. http://purl.obolibrary.org/obo/RO_0002351 has member http://purl.obolibrary.org/obo/BFO_0000051 has part has member is a mereological relation between a collection and an item. http://purl.obolibrary.org/obo/RO_0000087 has role http://purl.obolibrary.org/obo/RO_0000053 bearer of a relation between an independent continuant (the bearer) and a role, in which the role specifically depends on the bearer for its existence http://purl.obolibrary.org/obo/RO_0000081 role of http://purl.obolibrary.org/obo/RO_0000052 inheres in a relation between a role and an independent continuant (the bearer), in which the role specifically depends on the bearer for its existence http://purl.obolibrary.org/obo/RO_0002090 immediately precedes http://purl.obolibrary.org/obo/BFO_0000063 precedes http://purl.obolibrary.org/obo/IAO_0000039 has measurement unit label http://purl.obolibrary.org/obo/BFO_0000051 has part http://purl.obolibrary.org/obo/RO_0002087 immediately preceded by http://purl.obolibrary.org/obo/BFO_0000062 preceded by http://purl.obolibrary.org/obo/BFO_0000063 precedes http://purl.obolibrary.org/obo/RO_0002222 temporal relation http://purl.obolibrary.org/obo/RO_0002212 negatively regulates http://purl.obolibrary.org/obo/RO_0002211 regulates http://purl.obolibrary.org/obo/RO_0002213 positively regulates http://purl.obolibrary.org/obo/RO_0002211 regulates http://purl.obolibrary.org/obo/OBI_0002135 has specified value A relation between a value specification and a literal. http://purl.obolibrary.org/obo/IAO_0000136 is about A (currently) primitive relation that relates an information artifact to an entity. http://purl.obolibrary.org/obo/IAO_0000417 is quality measured as inverse of the relation of is quality measurement of http://purl.obolibrary.org/obo/OBI_0000124 is_supported_by_data The relation between a data item and a conclusion where the conclusion is the output of a data interpreting process and the data item is used as an input to that process http://purl.obolibrary.org/obo/OBI_0000417 achieves_planned_objective This relation obtains between a planned process and a objective specification when the criteria specified in the objective specification are met at the end of the planned process. http://purl.obolibrary.org/obo/OBI_0000833 objective_achieved_by This relation obtains between an objective specification and a planned process when the criteria specified in the objective specification are met at the end of the planned process. http://purl.obolibrary.org/obo/OBI_1110119 bound_to A relationship between two material entities that form a complex based on a selective, non-covalent interaction. http://purl.obolibrary.org/obo/RO_0000052 inheres in a relation between a specifically dependent continuant (the dependent) and an independent continuant (the bearer), in which the dependent specifically depends on the bearer for its existence http://purl.obolibrary.org/obo/RO_0000053 bearer of a relation between an independent continuant (the bearer) and a specifically dependent continuant (the dependent), in which the dependent specifically depends on the bearer for its existence http://purl.obolibrary.org/obo/RO_0000056 participates in a relation between a continuant and a process, in which the continuant is somehow involved in the process http://purl.obolibrary.org/obo/RO_0000057 has participant a relation between a process and a continuant, in which the continuant is somehow involved in the process http://purl.obolibrary.org/obo/RO_0000058 is concretized as A relationship between a generically dependent continuant and a specifically dependent continuant, in which the generically dependent continuant depends on some independent continuant in virtue of the fact that the specifically dependent continuant also depends on that same independent continuant. A generically dependent continuant may be concretized as multiple specifically dependent continuants. http://purl.obolibrary.org/obo/RO_0000059 concretizes A relationship between a specifically dependent continuant and a generically dependent continuant, in which the generically dependent continuant depends on some independent continuant in virtue of the fact that the specifically dependent continuant also depends on that same independent continuant. Multiple specifically dependent continuants can concretize the same generically dependent continuant. http://purl.obolibrary.org/obo/RO_0001000 derives from a relation between two distinct material entities, the new entity and the old entity, in which the new entity begins to exist when the old entity ceases to exist, and the new entity inherits the significant portion of the matter of the old entity http://purl.obolibrary.org/obo/RO_0001001 derives into a relation between two distinct material entities, the old entity and the new entity, in which the new entity begins to exist when the old entity ceases to exist, and the new entity inherits the significant portion of the matter of the old entity http://purl.obolibrary.org/obo/RO_0001015 location of a relation between two independent continuants, the location and the target, in which the target is entirely within the location http://purl.obolibrary.org/obo/RO_0001025 located in a relation between two independent continuants, the target and the location, in which the target is entirely within the location http://purl.obolibrary.org/obo/BFO_0000051 has part a core relation that holds between a whole and its part http://purl.obolibrary.org/obo/BFO_0000050 part of a core relation that holds between a part and its whole http://purl.obolibrary.org/obo/ECO_9000000 used_in A relation connecting an evidence type to an assertion method where the assertion method describes the type of process employed to evaluate the evidence. http://purl.obolibrary.org/obo/ECO_9000001 uses A relation that is an inverse of "used_in" relation. http://purl.obolibrary.org/obo/BFO_0000054 realized in http://purl.obolibrary.org/obo/BFO_0000055 realizes http://purl.obolibrary.org/obo/IAO_0000004 has measurement value http://purl.obolibrary.org/obo/BFO_0000062 preceded by http://purl.obolibrary.org/obo/RO_0002222 temporal relation http://purl.obolibrary.org/obo/RO_0002211 regulates