negatively regulates ribosome biogenesis negative regulation of cellular process biological regulation regulation of ribosome biogenesis negative regulation of ribosome biogenesis biological_process 2009-09-11T10:32:17Z GO:0090071 Any process that decreases the rate, frequency or extent of ribosome biogenesis. Ribosome biogenesis is the cellular process that results in the biosynthesis of constituent macromolecules, assembly, and arrangement of constituent parts of ribosome subunits.