has detection method is regulated by has measured entity has substrate has role has participant luminescence method reporter gene method Luciferin 4-monooxygenase luciferase induction Luciferase gene is attached to the regulatory sequence of a gene of interest. The effect of a perturbagen on the expression of the gene of interest could be easily measured by the detection of light emitted as a product the luciferase reaction. Adenosine triphosphate artificial regulatory region foreign promoter region luciferase gene reporter protein