image-based readout High content screening (HCS) is an automated screening method using live cells to understand various aspects of their biology. HCS employs imaging (bright-field or fluorescence) and analyses in a high throughput format. HCS can be applied to monitor complex cellular aspects, namely, signaling, morphology, phenotype, proliferation, motility, intracellular trafficking, toxicology, RNAi knockdown, and receptor activation. HCS High content screening single readout multiplexed readout assay readout content