genetic modification method transfection method This refers to the introduction of nucleic acids into cells using a construct. Plasmids constructs are created by the insertion of a gene or siRNA encoding DNA of interest into a plasmid. The engineered plasmids are introduced into cells by various means, namely, transfection, electroporation, nucleofection, etc which regulates the efficiency and location of gene transfer, for e.g., nucleofection delivers the plasmid directly into the nucleus in contrast to the other methods that deliver into the cytosol. silencing RNA delivery method