measure group specification assay readout parameter Since 'measurement' and 'parameter' are so similar in HCS, we need to decide on one term that says it all. assay readout parameter is the outcome of image analysis and is most often a calculated value. This includes 'parameters', such as 'intensity', 'count', 'morphology', etc which are all dependent on phenotype, e.g., intensity and count (number of bright spots) are a result of the amount of gene expression, and morphology is dependent on morphological phenotype, which is detected by the expression of a marker. measured parameter