has detection method has measured entity uses detection instrument has assay supporting method has assay supporting method bioassay chemiluminescence fold change molecular entity instrument GPCR Signaling Arrestin2 recruitment assay PRESTO-TANGO Beta-arrestin2 fused to the TEV protease liberates a tTA molecule fused to the C-terminus of a target G-protein coupled receptor when in close proximity, resulting in a tTA-driven expression of RLuc from a genomic TETO7-RLuc locus. chemiluminescent labeling method GPCR arrestin complex Spectramax L