Ptf1a{rtTA} mouse ESC line A mouse ESC line in which part or all of the targeted gene, pancreas specific transcription factor, 1a (NCBI Gene ID: 19213) is replaced to create Ptf1a{rtTA} using the Loxed Cassette Acceptor allele Ptf1a{tm1(LCA)}). These mESCs contain the reverse tetracycline transactivator (rtTA) under control of the Ptf1a regulatory sequences. rtTA is a transcriptional activator, which can inducibly (in the presence of the tetracycline analog doxycycline) activate the genes under Tet-O activator/promoter element. The rtTA sequences were introduced in to Ptf1a[LCA] by Recombinase-Mediated Cassette Exchange using standard methods.These cells were made in order togenerate a mouse line that express rtTA under control of the Ptf1a gene locus. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center stem cell derived cell line