Rosa26{Sox9.Cherry} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Sox9/mCherry using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This ES line contains bidirectional TetO-regulated genes inserted into the Rosa26.LCA allele by RMCE. In one direction the TetO/CMV promoter drives the expression of transcription factor Sox9. In the other direction it drives the expression of red fluorescent protein mCherry. This ES cell line can be used to simultaneously over-express Sox9 and mCherry upon administration of doxycyline when the effector protein rtTA is expressed. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center stem cell derived cell line