Rosa26{Setd5.GFP} mouse ESC line A mouse ESC line in which part or all of the targeted gene, gene trap ROSA 26, Philippe Soriano (NCBI Gene ID: 14910) is replaced to create Setd5/GFP using the Loxed Cassette Acceptor allele Rosa26{tm1(LCA)}). This ES cell line was generated by RMCE in the Rosa26{LCA} allele. A coding sequence for green fluorescent protein (GFP) was inserted into the first exon of the Setd5 gene. This exon is located upstream of the Rosa26 transcribed sequence and is transcribed in an opposite orientation. The GFP-polyA cassette, transcribed from the Setd5 promoter, knocks out Setd5 transcription and provides a reporter for Setd5 gene expression. The purpose of this cell line is to study the molecular function of the Setd5 gene. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center stem cell derived cell line