Nkx2.2{cCre-Knock-in} mouse ESC line A mouse ESC line in which part or all of the targeted gene, NK2 transcription factor related, locus 2 (NCBI Gene ID: 18088) is replaced to create Nkx2.2{Cre} using the Loxed Cassette Acceptor allele Nkx2.2{tm1(LCA)}). In this ES cell line, the sequence for one of the two bi-partite Cre molecules (cCre) and the IRES sequence were knocked into a site directly upstream of the Nkx2.2 start codon using RMCE in the Nkx2.2{LCA} allele. The presence of the IRES sequence allows for the transcription of cCre and Nkx2.2 from the native Nkx2.2 locus at the same time. This results in the labeling of pancreatic epithelium cells that co-express high levels of Ngn3 and Nkx2.2 during mouse embryonic development. Person: Mark A. Magnuson, Jill Lindner, Jean-Philippe Cartailler Vanderbilt University Medical Center stem cell derived cell line