has part is about has_specified_input has_specified_output has role ribonucleic acid gene expression information content entity evaluant role assay reverse transcribed polymerase chain reaction reverse transcription polymerase chain reaction assay GROUP: Penn Group PERSON: Chris Stoeckert, Jie Zheng an assay that evaluates the concentration of RNA in a sample in which an RNA strand is first reverse transcribed into its DNA complement (complementary DNA, or cDNA) using the enzyme reverse transcriptase, and the resulting cDNA is amplified using traditional or real-time PCR. reverse transcription polymerase chain reaction assay metadata complete