culture condition:tissue culture callus culture BTO:0004361 BrendaTissueOBO The callus culture is a technique of tissue culture, it is usually carried out on solidified gel medium in the presence of growth regulators and initiated by inoculation of small explants or sections from established organ or other cultures (the inocula). Plant cell calluses may be made to differentiate into the specialized tissues of a whole new plant, with the addition of a number of hormones or enzymes. This is an ability known as totipotency. Callus cultures can be indefinitely maintained through regular sub-culturing. callus cell culture