culture condition:-grown cell culture condition:McCoys 5A medium-grown cell 2016-09-25T09:40:58Z BTO:0005971 BrendaTissueOBO Marion, contact@brenda-enzymes.org McCoy's 5A medium was originally developed for the growth of Novikoff hepatoma cells. As modified by Iwakata and Grace, the increased levels of glucose, vitamins and peptone make McCoy's 5A a good general purpose medium for both primary, and established cell lines. Cells from biopsies (liver, intestine, skin, testes, bone marrow, spleen, lung and gingiva) have successfully been cultivated in this medium.