fluorescence laser scanning microscopy multi-photon laser scanning microscopy FIX:0000383 MPLSM Microscopy where the specimen can be made to fluoresce (emit energy as visible light) by scanning a solid-state (Ti sapphire) laser spot of specific wavelength over its surface to produce multi-photon fluorescence (i.e. the fluorophore simultaneously absorbs multiple photons of typically one half to one third of the energy required for single-photon excitation). multiphoton laser scanning microscopy CHMO:0000090 multi photon laser scanning microscopy