13C nuclear magnetic resonance spectroscopy distortionless enhancement with polarization transfer distortionless enhancement with polarisation transfer DEPT Spectroscopy where the energy states of 13C nuclei placed in a static magnetic field are interrogated by inducing transitions between the states via radio frequency irradiation. Each experiment consists of a sequence of radio frequency pulses with delay periods in between them.The 13C NMR signals are enhanced by transferring 1H magnetisation to 13C nuclei and the phase of the signals reflects the multiplicity i.e. CH, CH2 or CH3). Separate methyl, methylene and methine spectra may be obtained. distortionless enhancement by polarisation transfer distortionless enhancement by polarization transfer distortionless enhancement with polarization transfer CHMO:0000596 DEPT NMR