rotating frame Overhauser effect spectroscopy rOesy rotating frame Overhauser enhancement spectrometry ROESY CHMO:0000610 rotational Overhauser effect spectroscopy rotating frame Overhauser enhancement spectroscopy FIX:0000150 CAMELPSIN ROESY NMR cross-relaxation appropriate for minimolecules eμlated by locked spins rotating frame Overhauser effect spectrometry A type of heteronuclear NMR spectroscopy where a second frequency dimension is employed, based on the nuclear Overhauser enhancement (NOE) effect in which correlations are seen between nuclei that are spatially proximate within a molecule (⩽6 Å apart). Spin-locking is employed to ensure that correlation between spins, which are close in space but have zero NOE, are seen. This technique is used for molecules whose tumbling regime lies at the border of positive and negative NOE responses. two-dimensional nuclear Overhauser enhancement spectroscopy