light microscopy assay optical microscopy dark-field microscopy https://orcid.org/0000-0001-5985-7429 2009-07-16T02:28:55Z darkfield microscopy dark-field optical microscopy darkfield optical microscopy CHMO:0002177 Microscopy where the sample is illuminated with visible light that is not collected by the objective lens. Only the light scattered by the specimen is collected by the lens, producing an image of a light specimen on a black background.