fluorescence microscopy fluorescence microscopy assay epifluorescence microscopy 2009-07-21T10:34:34Z Microscopy where the specimen can be made to fluoresce (emit energy as visible light) by illuminating it with light of specific wavelengths. In epifluorescence microscopy light is transmitted through the objective lens onto the specimen, eliminating the need to filter out unwanted light directly from the source. https://orcid.org/0000-0001-5985-7429 CHMO:0002194