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    <!-- http://purl.obolibrary.org/obo/CHMO_0002277 -->

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        <rdfs:label>clear native polyacrylamide gel electrophoresis</rdfs:label>
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    <!-- http://purl.obolibrary.org/obo/CHMO_0002278 -->

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        <rdfs:label>quantitative preparative native continuous polyacrylamide gel electrophoresis</rdfs:label>
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        <oboInOwl:creation_date>2009-08-19T11:46:47Z</oboInOwl:creation_date>
        <oboInOwl:created_by>https://orcid.org/0000-0001-5985-7429</oboInOwl:created_by>
        <ns3:IAO_0000115>A separation method where metalloprotein molecules move at different speeds, in a buffer with pH 10, according to their charge and frictional forces in a separation medium consisting of polyacrylamide gel, across which an electric field is applied. This method does not use a dye to transfer charge to the protein molecules and the concentration of metal atoms from the metallproteins is determined by ICP-MS.</ns3:IAO_0000115>
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