step-wise elution chromatography stepwise-elution chromatography A separation method where the components are distributed between two phases, one of which is stationary, while the other moves in a definite direction (the 'mobile' phase). In stepwise elution chromatography the elution solvent strength of the mobile phase is increased during the separation as a series of discrete steps. CHMO:0002305 2009-08-24T04:26:19Z https://orcid.org/0000-0001-5985-7429 stepwise elution chromatography elution chromatography