fluorescence microscopy fluorescence microscopy assay optical interference microscopy reflection interference contrast microscopy Microscopy that determines the specimen's shape and distance away from a nearby, usually flat, reflective surface from the interference pattern produced by light reflecting from both the object and the surface. A microscope objective focusses a laser on the fluorescent specimen, and the same objective collects fluorescence. The interference between the fluorescence directly emitted by the specimen and the fluorescence reflected by the surface is observed by measuring the intensity of fluorescence passing through a confocal pinhole. https://orcid.org/0000-0001-5985-7429 reflection interference contrast (RIC-) microscopy reflection interference contrast (RIC) microscopy RIC microscopy 2009-09-22T05:31:44Z RICM CHMO:0002437