triple-resonance spectroscopy HNCO CHMO:0002556 Hils HNCO experiment 2009-10-25T08:08:23Z CHMO:0002057 HNCO A triple resonance NMR experiment used to determine the chemical shifts of nuclei in a (isotopically enriched) protein. The chemical shift of the (backbone) amide proton is correlated with the chemical shift of the covalently bound nitrogen and the carbonyl carbon of the preceding residue. The name of the method describes the path taken by the magnetisation during the experiment.