single isomorphous replacement single isomorphous replacement with anomalous scattering A method for providing phasing necessary for X-ray crystallography information by growing a crystal of the sample substituted with heavy metal atoms and analysing the X-ray data for both the original crystal and the isomorph. When the absorption edge of an atom in the crystals is close to the wavelength of the incident radiation the atom becomes an anomalous scatterer. Anomalous scattering can be used for phase determination and also for determining the absolute configuration. Hils SIRAS CHMO:0002728 FIX:0000248 2010-07-26T11:15:02Z