adjacent to intestinal crypt stem cell subepithelial intestinal fibroblast crypt-bottom fibroblast 2024-09-23T10:23:18Z bottom pericryptic-associated fibroblasts CBFs A subepithelial intestinal fibroblast that is located adjacent to the base of the crypt of Lieberkühn, near the intestinal stem cells. Characterized by low PDGFRα expression, this cell is crucial for maintaining the intestinal stem cell niche. Crypt-bottom fibroblast secretes key signaling molecules including canonical Wnt ligands (Wnt2, Wnt2b), Wnt potentiators (Rspo3), and BMP inhibitors (Grem1), which collectively regulate intestinal stem cell function and epithelial homeostasis. base of crypt of Lieberkuhn