COS-1 cell COS-1 细胞 NICR157 cell NICR157 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000157 48~103 Base Medium+8%DMSO+20%FBS C6 COS-1 COS-1 cell Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS NICR157 PMID: 8643633 Passage at 1: 4 ~ 1: 8; and change fluid once in 2~3 days. SV40-transformed Green Monkey Kidney Cells; COS-1 This cell is derived from a CV-1 cell line, obtained by transfection of SV40 encoding a wild-type T antigen with a starting point defect mutation; a single copy of the complete early segment of the SV40 genome is integrated. The cells express T antigen, suitable for transfection of vectors requiring expression of SV40 T antigen; the cells retain the properties of SV40 lytic growth and support replication of temperature sensitive A209 virus at 40 ℃, and support the transfection of SV40 pure body at early segment deletion. Because of the DNA sequence containing SV40, the cells need to be operated in a class 2 biosafety cabinet. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth fibroblast-like http://www.cellresource.cn/fdetail.aspx?id=157 http://www.cellresource.cn/pdf/20080108100710.pdf - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:4~1:8传代；2~3天换液1次。　 48~103 A类 C6 COS-1 细胞 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS PMID: 8643633 SV40转化的非洲绿猴肾细胞；COS-1 http://www.cellresource.cn/pdf/20080108100710.pdf 中国医学科学院基础医学研究所基础医学细胞中心 培养法（-） 基础培养基+8%DMSO+20%FBS 成纤维细胞样 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞源自CV-1细胞株，经转染编码野生型T抗原、起始点缺陷突变的SV40得到；细胞中整合有SV40基因组完整早期区段的单个拷贝。该细胞表达T抗原，适用于需要SV40T抗原表达的载体的转染；保留SV40溶细胞性生长的特性；支持40℃时温度敏感性A209病毒的复制；支持早期区段缺失的SV40纯体的复制。因含有SV40的DNA序列，该细胞需要在2级生物安全柜中操作。 贴壁生长