hFOB 1.19 cell hFOB 1.19 细胞 NICR473 cell NICR473 细胞 3131C0001000200014 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) qualified fetal bovine serum，10%；0.3mg/ml G418 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，10%；0.3mg/ml G418 Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Human SV40 Transfected Osteoblasts; hFOB 1.19 NICR473 Negative PN5 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). This cell line was established by transfection of fetal limb tissue of spontaneous abortionin with temperature-sensitive expression vector pUCSVisA58 in the presence of 0.6 mg/ml neomycin G418. Cell division was rapid at the permissible temperature of 33.5℃, while at the limit temperature of 39.5℃, the cells were little or no division. These cells have the ability to differentiate into mature osteoclasts that express the osteoblast phenotype. These cells provide a homogeneous rapid proliferation model system for studying the differentiation of normal human osteoblasts, the physiology and hormones of osteoblasts, growth factors, and cytokines that affect the function and differentiation of osteoblasts. adherent growth hFOB 1 19-cell morphology-SHCRC hFOB 1.19 hFOB 1.19 cell http://www.cellresource.cn/fdetail.aspx?id=473 http://www.cellresource.cn/pdf/20080108100542.pdf A类 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，10%；0.3mg/ml G418 PN5 SV40转染人成骨细胞；hFOB 1.19 hFOB 1 19-细胞形态-SHCRC hFOB 1.19 细胞 http://www.cellresource.cn/pdf/20080108100542.pdf 中国科学院上海生命科学研究院细胞资源中心 在0.6mg/ml新霉素G418存在下，用温度敏感的表达载体pUCSVisA58转染自然流产的胎儿四肢组织，建立了此细胞系。 在许可温度33.5oC下细胞分裂很快，而在限制温度39.5oC下细胞极少分裂或不分裂。 这些细胞具有分化为表达造骨细胞表型的成熟造骨细胞的能力。 这些细胞提供了一个同质化的快速增殖模型系统，用于研究正常人造骨细胞的分化，造骨细胞生理和激素，生长因子，和对造骨细胞的功能及分化有影响的细胞因子。 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性