hFOB 1.19 cell hFOB 1.19 细胞 NICR2160 cell NICR2160 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000487 Amelogenin：X；CSF1PO:10,13；D12S391:20,23；D13S317:11,12；D16S539:9,13；D18S51:10,17；D19S433:13,15；D21S11:29,32.2；D2S1338:23,24；D3S1358:17,18；D5S818:11,12；D6S1043:13,16；D7S820:8,10；D8S1179:10,14；FGA:19,22；Penta E:8,11；TH01:7,9.3；TPOX:11；vWA:16,18； Base Medium+8%DMSO+20%FBS Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) Human SV40 Transfected Osteoblasts; hFOB 1.19 NICR2160 Passage at 1: 2, and passage once in 3 days This cell line was established by transfection of fetal limb tissue of spontaneous abortionin with temperature-sensitive expression vector pUCSVisA58 in the presence of 0.6 mg/ml neomycin G418. Cell division was rapid at the permissible temperature of 33.5℃, while at the limit temperature of 39.5℃, the cells were little or no division. These cells have the ability to differentiate into mature osteoclasts that express the osteoblast phenotype. These cells provide a homogeneous rapid proliferation model system for studying the differentiation of normal human osteoblasts, the physiology and hormones of osteoblasts, growth factors, and cytokines that affect the function and differentiation of osteoblasts. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth hFOB 1.19 hFOB 1.19 cell http://www.cellresource.cn/fdetail.aspx?id=2160 http://www.cellresource.cn/pdf/20080108100542.pdf polygonal 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:2传代，3天传代一次 Amelogenin：X；CSF1PO:10,13；D12S391:20,23；D13S317:11,12；D16S539:9,13；D18S51:10,17；D19S433:13,15；D21S11:29,32.2；D2S1338:23,24；D3S1358:17,18；D5S818:11,12；D6S1043:13,16；D7S820:8,10；D8S1179:10,14；FGA:19,22；Penta E:8,11；TH01:7,9.3；TPOX:11；vWA:16,18； A类 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) SV40转染人成骨细胞；hFOB 1.19 hFOB 1.19 细胞 http://www.cellresource.cn/pdf/20080108100542.pdf 中国医学科学院基础医学研究所基础医学细胞中心 在0.6mg/ml新霉素G418存在下，用温度敏感的表达载体pUCSVisA58转染自然流产的胎儿四肢组织，建立了此细胞系。 在许可温度33.5oC下细胞分裂很快，而在限制温度39.5oC下细胞极少分裂或不分裂。 这些细胞具有分化为表达造骨细胞表型的成熟造骨细胞的能力。 这些细胞提供了一个同质化的快速增殖模型系统，用于研究正常人造骨细胞的分化，造骨细胞生理和激素，生长因子，和对造骨细胞的功能及分化有影响的细胞因子。 培养法（-） 基础培养基+8%DMSO+20%FBS 多角 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长