GH3 cell GH3 细胞 NICR8 cell NICR8 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000008 62~68 Base Medium+8%DMSO+20%FBS C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） F10: Ham＇s F10 Nutrient Mixture 2.5%FBS+15%HS GH3 GH3 cell GH3-cell morphology-PUMC NICR8 PMID: 5513559 Passage at 1: 2 ~ 1: 4; and passage for 2 to 3 times per week. Rat Pituitary Tumor Cells; GH3 The GH3 cell line was isolated from pituitary tumor of a 7-month-old female Wistar-Furth rat in July 1965 by Tashjian AH et al. The GH3 cell line was not directly derived from clones of the GH1 cell line, but was established from tumors formed by two passages of primarily cultured GH1 cells in rats. Epithelial-like GH3 cells secrete higher levels of growth hormone than GH1 and also produce prolactin. The research of regulating GH3 cell secretion of protein-like hormone showed that hydrocortisone can stimulate the secretion of growth hormone and inhibit the production of prolactin. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com epithelioid http://www.cellresource.cn/fdetail.aspx?id=8 http://www.cellresource.cn/pdf/20080108100736.pdf loosely adherent, floating clusters of cells - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:2~1:4传代；每周2~3次。 62~68 A类 C5 F10: Ham＇s F10 Nutrient Mixture 2.5%FBS+15%HS GH3 细胞 GH3-细胞形态-PUMC GH3细胞系是由Tashjian AH等在1965年7月从一只7月龄的雌性Wistar-Furth大鼠的垂体肿瘤中分离建立的。GH3细胞系不是直接来源于GH1细胞系的克隆，而是从原代培养的GH1细胞在大鼠身上传代两次形成的肿瘤中建立的。上皮样的GH3细胞比GH1分泌更高水平的生长激素，也可产生催乳素。对调控GH3细胞分泌蛋白类激素的研究表明，氢化可的松可以刺激生长激素的分泌、抑制催乳素的产生。 PMID: 5513559 http://www.cellresource.cn/pdf/20080108100736.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 培养法（-） 基础培养基+8%DMSO+20%FBS 大鼠垂体瘤细胞；GH3 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 疏松贴壁，有漂浮的细胞簇