NR8383 cell NR8383 细胞 NICR538 cell NICR538 细胞 3131C0001000500009 AgC11x3A AgC11x3A cell Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. F12K qualified fetal bovine serum，20% F12K 优质胎牛血清，20% NICR538 NR8383 NR8383 (normal rats, on Aug. 3, 1983) was derived from normal rat alveolar macrophages during lung lavage. The cells were cultured for approximately 8-9 months in the presence of gerbil lung cell continuous culture medium. Subsequently, exogenous growth factors are no longer needed. NR8383 cells were cloned and subcloned from single cells by limited dilution and subcloned three times with soft agar. The cells were characterized by macrophage characteristics, phagocytosis of yeast polysaccharide and cupric carbonate basic, non-specific lipase activity, Fc receptor, and oxidative degradation; The cells express IL-1,TNF beta and IL-6 secretion, and response repeately to exogenous growth factors. NR8383 cells respond to bleomycin and secrete TGF beta precursors. NR8383 cell NR8383.1 NR8383.1 cell Negative PN5 Passage at 1: 2. The cells can be overgrown within 3 days. Rat Alveolar Macrophages; NR8383[AgC11x3A; NR8383.1] The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). http://www.cellresource.cn/fdetail.aspx?id=538 http://www.cellresource.cn/pdf/20080108102725.pdf megakaryocyte semi-adherent growth 1:2。3天内可长满。 AgC11x3A 细胞 A类 F12K 优质胎牛血清，20% NR8383 (正常大鼠，1983年8月3日)来源于肺灌洗时的正常大鼠肺泡巨噬细胞。 细胞在gerbil肺细胞连续培养液存在下培养了大约8-9个月。 随后，不再需要外源生长因子。 通过有限稀释法从单个细胞克隆并亚克隆NR8383细胞，并三次用软琼脂亚克隆。 细胞表现出巨噬细胞的特性，吞噬酵母多糖和铜绿，非特异性脂酶活性，Fc受体，氧化降解；分泌IL-1, TNF beta和IL-6，可重复地响应外源生长因子。 NR8383细胞响应博莱霉素，分泌TGF beta前体。 NR8383 细胞 NR8383.1 细胞 PN5 http://www.cellresource.cn/pdf/20080108102725.pdf 中国科学院上海生命科学研究院细胞资源中心 半贴壁生长 基础培养基+8%DMSO+20%FBS 大鼠肺泡巨噬细胞；NR8383[AgC11x3A; NR8383.1] 巨噬细胞 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 阴性