L6 cell L6 细胞 NICR192 cell NICR192 细胞 - 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000192 38~40 Base Medium+8%DMSO+20%FBS C3 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS；4mM L-glutamine L6 L6 cell L6-Species Identification-PUMC L6-cell morphology-PUMC NICR192 Passage at 1: 20 ~ 1:40; and change fluid for 2~3 times a week. Rat Skeletal Muscle Myoblasts; L6 The cells were isolated by Yaffe from the first two generations of rat thigh muscle primary culture in the presence of methylcholanthrene; the cells fused in culture medium to form polynuclear myotubes and rhabdomyocytes and the fusion rate decreased with the generation increased, so the cells should be frozen for low generations and recloned periodically to select cells with strong fusion power. The cells are ectromelia virus-negative. The cells should be passaged before reaching confluent state to delay the loss of differentiation ability of the cells. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth http://www.cellresource.cn/fdetail.aspx?id=192 http://www.cellresource.cn/pdf/20080108100710.pdf myoblast-like - 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:20~1:40传代；每周换液2~3次。 38~40 A类 C3 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS；4mM L-glutamine L6 细胞 L6-物种鉴定-PUMC L6-细胞形态-PUMC http://www.cellresource.cn/pdf/20080108100710.pdf 中国医学科学院基础医学研究所基础医学细胞中心 培养法（-） 基础培养基+8%DMSO+20%FBS 大鼠骨骼肌成肌细胞；L6 成肌细胞样 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞是Yaffe在甲基胆蒽存在的情况下从大鼠大腿肌原代培养的最初两代细胞中分离得到的；在培养基中融合形成多核的肌管和横纹肌纤维，细胞融合的程度随着代数的增加而下降，因此细胞应低代次冷冻并周期性地重新克隆以选择融合能力强的细胞。鼠痘病毒阴性。该细胞应在达汇合状态前传代，以延缓细胞分化能力的丧失。 贴壁生长