L6 cell L6 细胞 NICR533 cell NICR533 细胞 3131C0001000500004 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. L6 L6 cell L6 myoblast strain Yaffe was isolated from first two generations of the primary culture of rat thigh muscle in the presence of methylcholanthrene. The L6 cells are fused in the medium to form polynuclear myotubes and rhabdomyocytes. The fusion degree of the cells decreases with the increase of the generation, so the cells should be frozen in the lower generation stage and recloned periodically to select cells with strong fusion ability. If the cells in the culture vessel are overgrown, the myogenic components of the cells will be rapidly depleted. Detection for the limb bone malformations virus ectromelia virus is negative. L6-cell morphology-SHCRC NICR533 Negative PN5 Rat Skeletal Muscle Myoblasts; L6 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth http://www.cellresource.cn/fdetail.aspx?id=533 http://www.cellresource.cn/pdf/20080108100710.pdf myoblast A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% L6 细胞 L6-细胞形态-SHCRC L6成肌细胞株是Yaffe从甲基胆蒽存在下大鼠大腿肌的原代培养物最初两代中分离得到。 L6细胞在培养基中融合形成多核的肌管和横纹肌纤维。 细胞融合的程度随着代数的增加而下降，因而细胞应冷冻于低代次阶段并周期性地重新克隆以选择融合能力强的细胞。 如果让培养容器中的细胞长满，这株细胞的成肌组分将迅速耗尽。 检测表明肢骨发育畸形病毒(鼠痘)阴性。 PN5 http://www.cellresource.cn/pdf/20080108100710.pdf 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 大鼠骨骼肌成肌细胞；L6 成肌细胞 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性