UMR-106 cell UMR-106 细胞 NICR1728 cell NICR1728 细胞 3131C0001001000011 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. NICR1728 Negative PN5 Rat Osteosarcoma Cells; UMR-106 The UMR-106 cell line was established by cloning the transplantable rat osteosarcoma induced by phosphorus radioisotope (32P). The cells respond to PTH, prostaglandins and osteoclast steroids. The response of UMR-106 to PTH was higher than that of UMR-108 (ATCC CRL-1663). Activation of protein kinase C inhibits ATP-induced elevation of the level of intracellular calcium. Both the starting osteosarcoma and the cloned strain were established by T.J. Martin of University of Sheffield. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). UMR-106 UMR-106 cell UMR-106-cell morphology-SHCRC adherent growth epithelial cell http://www.cellresource.cn/fdetail.aspx?id=1728 http://www.cellresource.cn/pdf/20080108100710.pdf A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS PN5 UMR-106 细胞 UMR-106-细胞形态-SHCRC http://www.cellresource.cn/pdf/20080108100710.pdf 上皮细胞 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 大鼠骨肉瘤细胞；UMR-106 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 注射放射性同位素磷(32P)诱导产生的可移植性大鼠骨肉瘤克隆建立了UMR-106细胞株。 细胞对PTH，前列腺素及破骨甾体有响应。 对PTH的响应度，UMR-106比相关细胞株UMR-108(ATCC CRL-1663)要高。 蛋白激酶C的活化抑制ATP诱导的胞内钙水平的升高。 起始骨肉瘤和克隆株都是University of Sheffield的T.J.Martin建立的。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性