Y3-Ag 1.2.3 cell Y3-Ag 1.2.3 细胞 NICR1589 cell NICR1589 细胞 3131C0001001200008 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class B Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS Maintain the cell concentration between 1 � 105 ~ 2 � 106cells/ml. NICR1589 Negative Rat Myeloma cells; Y3-Ag 1.2.3 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). This is a derived strain of Myeloma Cell Line S210 and was constructed by C. Milstein. Without the agreement of C. Milstein, the cells can not be sold or provided to the third party. This cell line is resistant to 8-azaguanine and sensitive to HAT, and can be fused with rat B cells to produce rat-rat hybridoma. Detection for limb bone malformations virus ectromelia virus is negative. Y3-Ag 1.2.3 Y3-Ag 1.2.3 cell http://www.cellresource.cn/fdetail.aspx?id=1589 http://www.cellresource.cn/pdf/20080108100710.pdf lymphoblast-like suspension growth B类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 10%FBS Y3-Ag 1.2.3 细胞 http://www.cellresource.cn/pdf/20080108100710.pdf 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 大鼠骨髓瘤细胞；Y3-Ag 1.2.3 悬浮生长 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 淋巴母细胞样 维持细胞浓度在1×105~2×106cells/ml。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 这是C. Milstein建立的骨髓瘤细胞株S210的衍生株。 未经C. Milstein同意不得销售或提供给第三方。 这株细胞具8-氮鸟嘌呤抗性，HAT敏感，可与大鼠B细胞融合生产大鼠-大鼠杂交瘤。 检测表明肢骨发育畸形病毒(鼠痘)阴性。 阴性