RBL-2H3 cell RBL-2H3 细胞 NICR764 cell NICR764 细胞 3131C0001001000007 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified heat-inactivatedfetal bovine serum，15% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质热灭活胎牛血清，15% NICR764 Negative PN5 RBL-2H3 RBL-2H3 cell RBL-2H3 is an alkaline leukemia cell line isolated and cloned from the tumor-preserving basophilic cells of Wistar rats by the Immunology Laboratory of the National Dental Research Institute in 1978. These cells have high affinity for IgE receptors. Histamine and other neurotransmitter secreted by these receptor can be activated by aggregation of these receptors or by synergism with calcium ionophore. This cell line is widely used to study the FcERI and secretion biochemical pathways of mast cells. RBL-2H3 cells are the research model for FcERI structure. They are widely used to study different aspects of cell secretion, including changes in intracellular calcium concentration, lipoxygenase activation, protein kinases and the action of small G proteins. Although almost all batches of FBS support cell growth, FcERI aggregates and de-particles are better in some batches . Another rat basophilic cell line (RBL, ATCC CRL-1378) is not de-granulated. Mycoplasma has been detected in this library. RBL-2H3-cell morphology Rat Basophilic Leukemia Cells; RBL-2H3 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth fibroblasts http://www.cellresource.cn/fdetail.aspx?id=764 http://www.cellresource.cn/pdf/20080108100951.pdf A类 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质热灭活胎牛血清，15% PN5 RBL-2H3 细胞 RBL-2H3-细胞形态 RBL-2H3是1978年国立牙科研究所的免疫学实验室从Wistar大鼠保持肿瘤状态的嗜碱性细胞中分离和克隆出来的嗜碱性白血病细胞株。 这些细胞具有高亲和力的IgE受体。 通过集聚这些受体或与钙离子载体协同作用可以激活它们分泌组胺及其他递质。 这株细胞广泛地用于研究肥大细胞FcERI和分泌的生化途径。 RBL-2H3细胞是研究FcERI结构的模型。 它们广泛地用于研究细胞分泌的不同方面，包括细胞内钙浓度改变、磷酸脂酶激活、蛋白激酶和小G蛋白的作用。 虽然几乎所有批号的FBS都支持细胞的生长，但在某些批号 http://www.cellresource.cn/pdf/20080108100951.pdf 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 大鼠嗜碱性粒细胞性白血病细胞；RBL-2H3 成纤维细胞 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性