CHO-DHFR - cell CHO-DHFR - 细胞 NICR456 cell NICR456 细胞 3131C0001000100004 Base Medium+8%DMSO+20%FBS CHO/dhFr- CHO/dhFr- cell Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Dihydrofolate Reductase-deficient Chinese Hamster Ovary Cells; CHO/dhFr- IMDM: Iscove＇s Modiefied Dulbecco＇s Medium qualified fetal bovine serum，10%；0.1 mM hypoxanthine, 0.016 mM thymidine, 100 nM methotrexate IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10%；0.1mM次黄嘌呤，0.016mM胸腺嘧啶，100nM氨甲喋呤 NICR456 Negative PN5 The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). This cell is a dihydrofolate reductase deficient cell. The cells will die in the absence of HT (hypoxanthine-thymine). The addition of methotrexate to the cell culture can prevent the growth of recurrent cells with low resistance to drugs. adherent growth epithelial cell http://www.cellresource.cn/fdetail.aspx?id=456 http://www.cellresource.cn/pdf/20080108100822.pdf A类 CHO/dhFr- 细胞 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10%；0.1mM次黄嘌呤，0.016mM胸腺嘧啶，100nM氨甲喋呤 PN5 http://www.cellresource.cn/pdf/20080108100822.pdf 上皮细胞 中国科学院上海生命科学研究院细胞资源中心 二氢叶酸还原酶缺陷型中国仓鼠卵巢细胞；CHO/dhFr- 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 该细胞为二氢叶酸还原酶缺陷细胞。 在没有HT（次黄嘌呤-胸腺嘧啶）时细胞会死亡。 细胞培液中加氨甲喋呤可以防止对药物低抗性的回变细胞的生长。 贴壁生长 阴性