Super Tube cell Super Tube 细胞 NICR1674 cell NICR1674 细胞 3131C0001000400029 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) qualified fetal bovine serum，10% DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，10% Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Dog Kidney Cells; Super Tube NICR1674 Negative PN5 Super Tube Super Tube cell The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). The cell line MDCK (ATCC CCL-34) was established from a normal adult female canker dog, from which the dog epithelioid cell line Super Tube was cloned. When maintaining a high cell concentration, Super tube formed a large number of tubules (it was reported that 7 x 105 cells placed on each well of a 24-well-plate were able to form tubules within 3 days). To form tubules, the cells culture medium needed to be exchanged twice a day to provide adequate nutrients. The detection level of c-AMP in Super Tube was much lower than that in the original cell line, and adenylyl cyclase was also lower under forskolin stimulation, and its transdermal resistance was also higher than that of Super Dome (ATCC CRL- 2286) and MOCK. adherent growth epithelial cell http://www.cellresource.cn/fdetail.aspx?id=1674 http://www.cellresource.cn/pdf/20080108100542.pdf A类 DMEM/F12(1:1): Ham＇s F12/DME Medium (DME H-16/F-12 50% Mixture w/o HEPES, with NEAA Medium) 优质胎牛血清，10% PN5 Super Tube 细胞 http://www.cellresource.cn/pdf/20080108100542.pdf 上皮细胞 中国科学院上海生命科学研究院细胞资源中心 从表观正常的成年雌性可卡犬中建立了细胞株MDCK (ATCC CCL-34)，从中克隆建立了狗上皮样细胞株Super Tube。 当维持高细胞浓度时，Super tube形成大量的小管(据报道，24孔板的每孔铺7x105细胞，３天内就能形成小管)。 要形成小管，细胞需要每天两次换液以提供足够养分。 与原始细胞株相比，Super Tube的c-AMP的检测水平低得多，在forskolin刺激下读出的腺苷环化酶水平也低；它的跨上皮抗性也比Super Dome (ATCC CRL- 2286) 和MDCK都低。 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 狗肾细胞；Super Tube 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性