DT40 cell DT40 细胞 NICR757 cell NICR757 细胞 3131C0001000900002 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Chicken B Lymphoma Cells; DT40 Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10%，chicken serum，5%；0.05mM β-mercaptoethanol DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10%，鸡血清，5%；0.05mM β-巯基乙醇 DT40 DT40 cell DT40 was derived from the bursal lymphocyte strain of Hyline SC induced by avian leukosis virus. Primary lymphoma was obtained from one day old chickens infectied with Roche-associated virus 1 (RAV-1). The tumors generated in the bursa of Fabricius were made into cell suspension and then injected intravenously into receptor chickens of the same genotype. After one transplantation in vivo, DT40 cell line was established. The cells contains proviral genes that are integrated upstream of c-myc proto-oncogene and express high levels of c-myc RNA. It lacks a normal c-myc gene, but contains myc gene that are regulated by two copies of ALV. This cell line retains the ability to rearrange the immunoglobulin light chain gene (IgL). At the IgL site, DT40 contains a heavy chain. NICR757 Negative PN5 Passage at 1: 2. The cells can be overgrown within 3 days. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). http://www.cellresource.cn/fdetail.aspx?id=757 http://www.cellresource.cn/pdf/20080108100710.pdf lymphoblast suspension growth 1:2。3天内可长满。 A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10%，鸡血清，5%；0.05mM β-巯基乙醇 DT40 细胞 DT40是来自Hyline SC鸡法氏囊淋巴细胞株经鸟类白血病病毒诱导建株的。原始淋巴瘤用罗氏相关病毒1(RAV-1)感染出生１天的小鸡得到。 法氏囊中生成的肿瘤制成细胞悬液后通过静脉注射输入同基因型的受体小鸡。 经过一次体内移植后，建立了DT40细胞株。 这株细胞包含的前病毒基因整合在c-myc原癌基因的上游，表达的c-myc RNA水平较高。 它缺少一个正常c-myc基因，但含有两个拷贝ALV去调控的myc基因。 这株细胞保留了重排免疫球蛋白轻链基因(IgL)的能力。 在IgL位点，DT40包含一个重和一个胚系同源基因。 c-rel基因和v-rel癌基因在DT40细胞株中都能诱导组织相容性(MHC)II类抗原表达。 v-rel 诱导的MHCII表达比c-rel诱导快，且其有效性在数周后达到c-rel的50倍。 这株细胞呈淋巴母细胞表型。 传染性检测表明DT40释放低水平的传染性RAV-1。这株细胞可以用于稳转研究。 PN5 http://www.cellresource.cn/pdf/20080108100710.pdf 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 悬浮生长 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 淋巴母细胞 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 阴性 鸡淋巴瘤细胞；DT40