UMNSAH/DF-1 cell UMNSAH/DF-1 细胞 NICR1675 cell NICR1675 细胞 3131C0001000400030 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) qualified fetal bovine serum，10% DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% Digestion for 3-5 minutes. Passage at 1: 2. The cells can be overgrowth within 3 days. Embryonic Chicken Fibroblasts; UMNSAH/DF-1 NICR1675 Negative P(150+5) The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). UMNSAH DF-1-cell morphology-SHCRC UMNSAH/DF-1 UMNSAH/DF-1 cell UMNSAH/DF-1 is a chicken cell line derived from 10-day-old ELL-0 eggs and turns to immortalized cells spontaneously. Primary chicken embryo fibroblasts were isolated and cultured in a culture medium; the cells were passaged until aging; the cells were centrifuged to keep cell culture at a coverage of 30% to 60% during aging; non-aging clones were identified and passaged at least 30 times. No clonal proliferation was observed on soft agar, suggesting that these cells were immortalized without transformation. This cell line can serve as a matrix for virus proliferation, recombinant protein expression and recombinant virus production. adherent growth fibroblasts http://www.cellresource.cn/fdetail.aspx?id=1675 http://www.cellresource.cn/pdf/20080108100710.pdf A类 DMEM-H: Dulbecco＇s Modified Eagle＇s Medium (DME H-21 4.5g/Liter Glucose) 优质胎牛血清，10% P(150+5) UMNSAH DF-1-细胞形态-SHCRC UMNSAH/DF-1 细胞 UMNSAH/DF-1是起源于10日龄的ELL-0鸡蛋的鸡细胞株，自发永生化。 分离原代鸡胚成纤维细胞并在培养液中培养；传代直到衰老；在衰老过程中离心以保持细胞培养在30%到60%满；不衰老的克隆进行鉴定并传代不少于30次。 在软琼脂上没有观察到克隆增殖，说明这些细胞是永生化而没有转化。 这株细胞可作为病毒增殖、重组蛋白表达和重组病毒生产的基质。 http://www.cellresource.cn/pdf/20080108100710.pdf 中国科学院上海生命科学研究院细胞资源中心 基础培养基+8%DMSO+20%FBS 成纤维细胞 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化3-5分钟。1:2。3天内可长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 贴壁生长 阴性 鸡胚成纤维细胞；UMNSAH/DF-1