MDCK cell MDCK 细胞 NICR417 cell NICR417 细胞 3111C0002000000045 Base Medium+8%DMSO+20%FBS Canine Kidney Cells; MDCK(NBL-2) Class A Culture Method（-） In 1958, S.H. Madin and N.B. Darby established MDCK cell lines from the kidneys of normal adult female Cocker Spaniel. The cells are positive for keratin by immunoperoxidase staining. The cells were used to study the formation of β-amyloid precursor proteins and the classification their proteolytic products. MDCK(NBL-2) MDCK(NBL-2) cell MDCK(NBL-2)-cell morphology-NIFDC MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) new-born calf serum，10% MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% NICR417 National Institute for Food and Drug Control P63 Passage at 1: 2 ~ 1: 6 Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net adherent growth epithelium-like; polygon http://www.cellresource.cn/fdetail.aspx?id=417 http://www.cellresource.cn/pdf/20080108100951.pdf 1958年，Madin SH和Darby NB从表观正常的成年雌性科克猎犬的肾建立了MDCK细胞株。角蛋白阳性。该细胞曾被用于研究β淀粉前体蛋白的形成及其蛋白水解产物的分类。 1：2~1：6 A类 MDCK(NBL-2) 细胞 MDCK(NBL-2)-细胞形态-NIFDC MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 新生牛血清，10% P63 http://www.cellresource.cn/pdf/20080108100951.pdf 上皮样；多角 中国食品药品检定研究院 培养法（-） 基础培养基+8%DMSO+20%FBS 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 犬肾细胞；MDCK(NBL-2) 贴壁生长