MDCK cell MDCK 细胞 NICR780 cell NICR780 细胞 3142C0001000000012 Base Medium+8%DMSO+20%FBS C75 Canine Kidney Cells; MDCK(NBL-2) Cell Library of China Center For Type Culture Collection Class A Congyi Zheng, Sanfu Qu, Cell Library of China Center For Type Culture Collection, Wuhan University, 430072, 027－68754712, Fax:027－68754833, cctcc@whu.edu.cn In 1958, S.H. Madin and N.B. Darby established MDCK cell lines from the kidneys of normal adult female Cocker Spaniel. The cells are positive for keratin by immunoperoxidase staining. The cells were used to study the formation of β-amyloid precursor proteins and the classification their proteolytic products. Isoenzyme Identification Chromosome MDCK(NBL-2) MDCK(NBL-2) cell MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS NICR780 Negative Passage at 1: 3, and passage once in 2-3 days. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). adherent growth epithelial-like http://www.cellresource.cn/fdetail.aspx?id=780 http://www.cellresource.cn/pdf/20080108100951.pdf 1958年，Madin SH和Darby NB从表观正常的成年雌性科克猎犬的肾建立了MDCK细胞株。角蛋白阳性。该细胞曾被用于研究β淀粉前体蛋白的形成及其蛋白水解产物的分类。 1:3传代,2-3天传一代 A类 C75 MDCK(NBL-2) 细胞 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 10%FBS http://www.cellresource.cn/pdf/20080108100951.pdf 上皮细胞样 中国典型培养物保藏中心细胞库 同工酶鉴定 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 犬肾细胞；MDCK(NBL-2) 贴壁生长 郑从义，屈三甫 武汉大学中国典型培养物保藏中心，430072，027－68754712传真027－68754833，cctcc@whu.edu.cn 阴性