HMy2.CIR cell HMy2.CIR 细胞 NICR479 cell NICR479 细胞 3131C0001000200021 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. HMy2.CIR HMy2.CIR cell Human B Lymphoblast Cells; HMy2.CIR IMDM: Iscove＇s Modiefied Dulbecco＇s Medium qualified fetal bovine serum，10% IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10% NICR479 Negative PN5 Passage at 1: 2. The cells can be overgrown within 3 days. The HMy2.CIR cell line was obtained from the rapid growth mutant HMy. 2B of ARH-77 cell line irradiated by γ-ray, in which the cells lacking the expression of HLA class I antigen were selected. This cell line did not express the products of HLA in site A and site B, but expressed a small amount of HLA Cw4. This cell strain is suitable for used as a transfected host for class I major histocompatibility complex gene. It is reported that ARH-77 cell line is positive in EB nuclear antigen (EBNA +) test and positive in EB virus capsule antigen (EBVCA +)test. Hmy2.CIR cell line originated from the fast-growing mutant HMy. 2 B of ARH-77 cell line, and It is also presumed that Hmy2.CIR cell line is positive in EB nuclear antigen (EBNA +) test. The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). http://www.cellresource.cn/fdetail.aspx?id=479 http://www.cellresource.cn/pdf/20080108100822.pdf lymphoblast suspension growth 1:2。3天内可长满。 ARH-77细胞株的快速生长突变株HMy.2 B用γ射线照射，选择HLA I型抗原表达缺失的细胞，得到HMy2.CIR细胞株。 这株细胞不表达HLA A位点和B位点的产物，但表达少量HLA Cw4。 这株细胞适于用作I型主要组织相容性抗原基因的转染宿主。 有报道称ARH-77细胞株呈EB核抗原阳性(EBNA+)和EB病毒荚膜抗原阳性(EBVCA+)。 由于Hmy2.CIR细胞株起源于ARH-77细胞株的快速生长突变株HMy.2 B，推测它也是EBNA+。 A类 HMy2.CIR 细胞 IMDM: Iscove＇s Modiefied Dulbecco＇s Medium 优质胎牛血清，10% PN5 http://www.cellresource.cn/pdf/20080108100822.pdf 中国科学院上海生命科学研究院细胞资源中心 人B淋巴母细胞；HMy2.CIR 基础培养基+8%DMSO+20%FBS 悬浮生长 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 淋巴母细胞 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 阴性