RAMOS cell RAMOS 细胞 NICR47 cell NICR47 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000047 38~40 Base Medium+8%DMSO+20%FBS C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Burkitt Lymphoma B Cells; RAMOS Maintain the cell concentration between 2 � 105/ml ~ 1 � 106/ml; and replenish the fluid according to the cell concentration every 2 to 3 days. NICR47 PMID: 15562757 RAMOS RAMOS cell RPMI 1640 (w/o Hepes) 10%FBS The cells are derived from a 3-year-old white Burkitt lymphoma patient and are EBV-negative; the cells express IL-4 receptor and low affinity IgE receptor (CD23); there are about 1500 IL-4 binding sites on each cell surface; the cells secrete IgM (lamda L) and express membrane and secretory immunoglobulin. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com http://www.cellresource.cn/fdetail.aspx?id=47 http://www.cellresource.cn/pdf/20080108101040.pdf lymphoblast-like suspension growth 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 38~40 A类 C5 PMID: 15562757 RAMOS 细胞 RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf 中国医学科学院基础医学研究所基础医学细胞中心 人B淋巴细胞瘤细胞；RAMOS 培养法（-） 基础培养基+8%DMSO+20%FBS 悬浮生长 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 淋巴母细胞样 维持细胞浓度在2×105/ml~1×106/ml；根据细胞浓度每2~3天补液1次。 该细胞来源于一位3岁的白人Burkitt淋巴瘤患者；EBV阴性；表达IL-4受体和低亲和性IgE受体（CD23）；每个细胞表面大约有1500个IL-4的结合位点；分泌IgM（lamda L）；表达膜型和分泌型的免疫球蛋白。