has_quality has participant MEG-01 cell MEG-01 细胞 NICR651 cell NICR651 细胞 3131C0001000700083 Base Medium+8%DMSO+20%FBS Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class B Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Human Megakaryocytic Leukemia Cells; MEG-01 MEG-01 MEG-01 cell MEG-01 cell line is derived from bone marrow cells of a CML patient during megakaryocytic transformation. Tests for Cytoplasmic Factor VIII and surface globulin IIb/IIIa, Periodic acid-Schiff (PAS) reaction, α-naphthyl acetate esterase and acid phosphatase are positive. Tests for myeloperoxidase, α-naphthyl butyrate esterase, acetic acid AS-D naphthyl phenol esterase and alkaline phosphatase are negative. Staining with monoclonal antibodies BA-1 (anti-B cells, granulocyte), HPL-3 (anti-globulin IIb/IIIa) and 20.3 (anti-monocytes, platelets) was positive. Tests for other lymphoid and bone marrow antibodies are negative. NICR651 Passage at 1: 2. The cells can be overgrown within 3 days. Positive RPMI 1640 (w/o Hepes) qualified fetal bovine serum，10% RPMI 1640 (w/o Hepes) 优质胎牛血清，10% The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). U http://www.cellresource.cn/fdetail.aspx?id=651 http://www.cellresource.cn/pdf/20080108101040.pdf lymphoblast-like suspension growth 1:2。3天内可长满。 B类 MEG-01 细胞 MEG-01细胞株源自一位CML患者成巨核细胞转换期的骨髓细胞。 细胞质因子VIII和表面球蛋白IIb/IIIa，高碘酸-Schiff(PAS)反应，α醋酸萘酯酶和酸性磷酸酶阳性。 髓过氧化物酶，α丁酸萘酯酶，氯化醋酸AS-D萘苯酚酯酶和碱性磷酸酶阴性。 用单克隆抗体BA-1(抗B细胞，粒性白细胞)，HPL-3(抗球蛋白IIb/IIIa)和20.3(抗单核细胞，血小板)染色成阳性。 其他淋巴和骨髓类抗体成阴性。 RPMI 1640 (w/o Hepes) 优质胎牛血清，10% U http://www.cellresource.cn/pdf/20080108101040.pdf 中国科学院上海生命科学研究院细胞资源中心 人成巨核细胞白血病细胞；MEG-01 基础培养基+8%DMSO+20%FBS 悬浮生长 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 淋巴母细胞样 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 阳性 positive 阳性 alkaline phosphatase