GLC-82 cell GLC-82 细胞 NICR67 cell NICR67 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000067 62~64 Amelogenin：X；CSF1PO：9，10；D13S317：12，13.3；D16S539：9，10；D18S51：16；D19S433：13，14；D21S11：27，28；D2S1338：17；D3S1358：18；D5S818：11，12；D7S820：8，12；D8S1179：12，13；FGA：21；TH01：7；TPOX：8，12；vWA：16，18； Base Medium+8%DMSO+20%FBS C5 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） GLC-82 GLC-82 cell GLC-82-Species Identification-PUMC GLC-82-cell morphology-PUMC GLC82 GLC82 cell Low Differentiation Human Lung Adenocarcinoma Cells; GLC-82 [GLC82] NICR67 Passage at 1: 5 ~ 1:10, the proposed initial concentration is 2 ~ 4 � 104 cells/cm2; and change fluid once in 2~3 days. RPMI 1640 (w/o Hepes) 10%FBS The cells are derived from a 58-year-old Chinese woman with lung adenocarcinoma; the primary cultured cell began to grow on the 7th day, and was first passage after 84 days and cultured for 24 months; the cells were polygonal; immunosuppressed Kunming mice and Wistar rats were inoculated subcutaneously successfully. STR detection revealed that the cells were contaminated by HeLa cells. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelial-like http://www.cellresource.cn/fdetail.aspx?id=67 http://www.cellresource.cn/pdf/20080108101040.pdf 张咏, and 王阁. "重组人肝细胞生成素的生物学性能." 生理学报 51.3(1999):347-350. 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:5~1:10传代，建议初始浓度为 2~4×104 cells/cm2；2~3天换液1次。　 62~64 Amelogenin：X；CSF1PO：9，10；D13S317：12，13.3；D16S539：9，10；D18S51：16；D19S433：13，14；D21S11：27，28；D2S1338：17；D3S1358：18；D5S818：11，12；D7S820：8，12；D8S1179：12，13；FGA：21；TH01：7；TPOX：8，12；vWA：16，18；　 A类 C5 GLC-82 细胞 GLC-82-物种鉴定-PUMC GLC-82-细胞形态-PUMC GLC82 细胞 RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf 上皮细胞样 中国医学科学院基础医学研究所基础医学细胞中心 人低分化肺腺癌细胞；GLC-82 [GLC82] 培养法（-） 基础培养基+8%DMSO+20%FBS 张咏, and 王阁. "重组人肝细胞生成素的生物学性能." 生理学报 51.3(1999):347-350. 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞源自一位患有肺腺癌的58岁的中国女性；原代培养，7天细胞开始生长，首次传代84天，培养24个月；该细胞呈多边形；免疫抑制昆明小鼠及Wistar断奶大鼠癌细胞悬液皮下接种成功。STR检测发现该细胞被HeLa细胞污染。 贴壁生长