NK-92MI cell NK-92MI 细胞 NICR437 cell NICR437 细胞 3111C0002000000065 Amelogenin:X,Y；CSF1PO:11,12；D13S317:9,12；D16S539:11,12；D18S51:12,17；D21S11:31.2,32；D3S1358:15；D5S818:12,13；D7S820:10,11；D8S1179:12,15；FGA:20,22；PentaD:10,12；PentaE:12；TH01:6,9.3；TPOX:8；vWA:16,18 Base Medium+8%DMSO+20%FBS Class A Culture Method（-） NICR437 NK-92 is an interleukin-2 dependent NK cell line derived from peripheral blood monocytes of a 50-year-old white male with acute non-Hodgkin's lymphoma. NK-92MI is an IL-2 independent NK cell line derived from NK-92 transfected cells. The parent cells were transformed with human IL-2 cDNA carried by the retroviral MFG-hIL-2 vector used in microsomal gene transformation.NK-92MI is an IL-2 independent NK cell line derived from NK-92 transfected cells. The parent cells were transformed with human IL-2 cDNA carried by the retroviral MFG-hIL-2 vector used in microsomal gene transformation. Possibly due to the integration of the vector into the genomic DNA, the transformation is stable. This cell is cytotoxic to many malignant cells; chromium release tests show that it can kill K562 and Daudi cells. NK-92 cells have the following characteristics: surface markers of CD2, CD7, CD11a, CD28, CD45 is positive, CD56 bright; surface markers of CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34 and HLA-DR is negative. NK-92MI NK-92MI cell NK-92MI-cell morphology-NIFDC NK92MI NK92MI cell National Institute for Food and Drug Control Natural Killer Cells in Patients with Malignant Non-hodgkin Lymphoma; NK-92MI [NK92MI] Passage at 1: 2. The cells can be overgrown within 3 days. Shufang Meng, Xiang Zhao, Tel:67095414, 67095360, Email:mengsf@263.net http://www.cellresource.cn/fdetail.aspx?id=437 http://www.cellresource.cn/pdf/20080108100503.pdf lymphoblast-like suspension growth α－MEM：Alpha Minimum Essential Medium (with Nucleosides) horse serum， 12.5% qualified fetal bovine serum，120.005 α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 马血清， 12.5%；优质胎牛血清，120.005 1:2。3天内可长满。 Amelogenin:X,Y；CSF1PO:11,12；D13S317:9,12；D16S539:11,12；D18S51:12,17；D21S11:31.2,32；D3S1358:15；D5S818:12,13；D7S820:10,11；D8S1179:12,15；FGA:20,22；PentaD:10,12；PentaE:12；TH01:6,9.3；TPOX:8；vWA:16,18 A类 NK-92MI 细胞 NK-92MI-细胞形态-NIFDC NK-92是从一位患有急进性非霍奇金淋巴瘤的50岁白人男性外周血单核细胞衍生来的一株白细胞介素-2依赖型NK细胞株。 这株细胞对很多恶性细胞有细胞毒性；铬释放试验显示它能杀死K562和Daudi细胞。 NK-92细胞(经过照射以防止增殖)可以有效地用于血液中白血病的体外免疫清扫而不危及血细胞的功能。 NK-92细胞有以下特征： CD2, CD7, CD11a, CD28, CD45, CD54表面标记阳性，CD56亮；CD1, CD3, CD4, CD5, CD8, CD10, CD14, CD16, CD19, CD20, CD23, CD34和HLA-DR表面标记阴性。 NK92MI 细胞 http://www.cellresource.cn/pdf/20080108100503.pdf α－MEM：Alpha Minimum Essential Medium (with Nucleosides) 马血清， 12.5%；优质胎牛血清，120.005 中国食品药品检定研究院 人恶性非霍奇金淋巴瘤患者的自然杀伤细胞；NK-92MI [NK92MI] 培养法（-） 基础培养基+8%DMSO+20%FBS 孟淑芳，赵翔， Tel:67095414,67095360, Email:mengsf@263.net 悬浮生长 淋巴母细胞样