has_quality has participant expresses NCI-H23 cell NCI-H23 细胞 NICR255 cell NICR255 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000255 Base Medium+8%DMSO+20%FBS Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Non-small Cell lung cancer Cells; NCI-H23 NCI-H23 NCI-H23 cell NCI-H23-Species Identification-PUMC NICR255 Passage at 1: 3; and passage once in 3 to 4 days. RPMI 1640 (w/o Hepes) 10%FBS；4.5g/L Glucose＋10mMHepes＋1mM sodium pyruvate This cell is derived from the tumor tissue of a 51-year-old black patient with non-small cell lung cancer before treatment, expressing C-myc, L-myc, v-src, v-abl, v-erb B, c-raf 1, Ha-ras, Ki-ras, and N-ras RNAs; this cell carries the K-ras 12 mutation; mutation at codon 246 of p53 gene is ATC → ATG; this cell express Heterologous mRNA of PDGF A and B chains, TGF α, TGF β and EGFR; detection for keratin 5, 8 and 18 is positive. the cells are vimentin-positive, neurofilament protein-positive and L-dopa dehydrogenase-negeative. it is reported that the efficiency of cloning of the cells in soft agar is 9.7%. Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=255 http://www.cellresource.cn/pdf/20080108101040.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1:3传代；3~4天1次。 A类 NCI-H23 细胞 NCI-H23-物种鉴定-PUMC RPMI 1640 (w/o Hepes) 10%FBS；4.5g/L Glucose＋10mMHepes＋1mM sodium pyruvate http://www.cellresource.cn/pdf/20080108101040.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 人非小细胞肺癌细胞；NCI-H23 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 该细胞源于一位51岁患有非小细胞肺癌黑人男性患者的治疗前的肿瘤组织，表达C-myc、L-myc、v-src、v-abl、v-erb B、c-raf 1、Ha-ras、Ki-ras、N-ras RNAs；该细胞携带K-ras 12突变；p53基因246位密码子突变ATC→ATG；表达PDGF A和B链的异源mRNA；表达TGFα、TGFβ和EGFR；角蛋白 5、8和18阳性，波形蛋白阳性，神经丝蛋白阴性，左旋多巴脱氢酶阴性；据报道，在软琼脂中该细胞形成克隆的效率为9.7%。 贴壁生长 positive 阳性 epidermal growth factor receptor 表皮生长因子受体 keratin, type I cytoskeletal 18 vimentin