NCI-H358 cell NCI-H358 细胞 NICR2143 cell NICR2143 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000470 Amelogenin:X,Y；CSF1PO:11，12；D13S317:8，12；D16S539:12，13；D18S51:14；D19S433:13，14；D21S11:28，30；D2S1338:17，23；D3S1358:14，18；D5S818:10,12；D7S820:10，11；D8S1179:13，14；FGA:20,21；TH01:6；TPOX:8，9；vWA:17 Base Medium+8%DMSO+20%FBS Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Non-small Cell lung cancer Cells; NCI-H358 In 1981, the cell line was isolated from tumor tissue of a patient before chemotherapy. Ultrastructural studies showed that the cytoplasm was of Clara cells feature structure expressing the major protein and RNA of pulmonary surfactant associate protein A, SP-A on lung surface. SP-B and SP-C were not expressed. The clone formation efficiency in soft agar was 0.83%. NCI-H358 NCI-H358 cell NICR2143 Passage at 1: 2; and passage once within 3 days. RPMI 1640 (w/o Hepes) 10%FBS Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelium-like; polygonal shape http://www.cellresource.cn/fdetail.aspx?id=2143 http://www.cellresource.cn/pdf/20080108101040.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1981年从一位开始化疗之前的患者的肿瘤组织中分离建株。 超微结构研究表明细胞质中有Clara细胞的特征结构 细胞表达主要的肺表面结合蛋白SP-A的蛋白和RNA。 不表达SP-B和SP-C。 他们在软琼脂中的克隆形成效率为0.83%。 1:2传代；3天传代一次。 Amelogenin:X,Y；CSF1PO:11，12；D13S317:8，12；D16S539:12，13；D18S51:14；D19S433:13，14；D21S11:28，30；D2S1338:17，23；D3S1358:14，18；D5S818:10,12；D7S820:10，11；D8S1179:13，14；FGA:20,21；TH01:6；TPOX:8，9；vWA:17 A类 NCI-H358 细胞 RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf 上皮样；多角形 中国医学科学院基础医学研究所基础医学细胞中心 人非小细胞肺癌细胞；NCI-H358 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长