has participant NCI-H520 cell NCI-H520 细胞 NICR197 cell NICR197 细胞 1. Cell Resource Center of Peking Union Medical College provides experimental cell technology services for scientific research. The provided experimental cells and their offspring can not be used in human experiments and clinical diagnosis and treatment, and can not be used for commercial activities directly or indirectly. 2. Cell Resource Center of Peking Union Medical College is committed to doing its utmost to update experimental cell resources in a timely fashion. But limited to the technical conditions at the time, the center does not guarantee its accuracy. 3. The Center did not verify information cited from the literature, etc, for reference purposes only. 4. Users should observe the relevant laws and regulations at home and abroad when receiving, handling, preserving, discarding, transferring and using cells, taking into account the risks and liabilities that may exist and taking appropriate safety and handling measures to minimize hazards to health or the environment. 3111C0001CCC000197 91~95 Base Medium+8%DMSO+20%FBS C6 Cell Culture Center, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences Class A Culture Method（-） Human Lung Squamous Carcinoma Cells; NCI-H520 It was established from lung squamous cell carcinoma by Gazdar AF in 1982; the expression level of p53 mRNA was lower than that of normal lung tissue, but there is no big structural DNA anomaly. The cells is keratin-positive, vimentin-positive, NF3(neurofilament triple protein)-negative; the cells can form clones in soft agar. NCI-H520 NCI-H520 cell NCI-H520-Species Identification-PUMC NCI-H520-cell morphology-PUMC NICR197 Passage at 1: 3 ~ 1: 6; and change fluid once in 2~3 days. RPMI 1640 (w/o Hepes) 10%FBS Zhenli Yang, Yuqin Liu, Tel:65286441, 69156455, Email:crcpumc@vip.163.com adherent growth epithelioid http://www.cellresource.cn/fdetail.aspx?id=197 http://www.cellresource.cn/pdf/20080108101040.pdf 1. 协和细胞资源中心为科学研究提供实验细胞技术服务。所提供的实验细胞及其子代，不能用于人体实验和临床诊断、治疗，不能直接或间接用于商业行为。 2. 协和细胞资源中心承诺尽最大努力对实验细胞资源相关信息进行及时更新。但限于当时的技术条件，中心不保证其准确性。 3. 从文献等处引用的信息本中心未进行核实，仅供参考。 4. 使用者在接收、处理、保存、丢弃、转让及使用细胞的时候要遵守国内外有关的法律法规，充分考虑可能存在的风险和责任，采取适当的安全和处理措施尽量降低对健康或环境的危害 1982年Gazdar AF等建系，源于鳞状细胞肺癌组织；该细胞p53 mRNA表达水平较正常肺组织低，但是没有大的结构DNA的异常。角蛋白、波形蛋白阳性，神经丝三联蛋白阴性；该细胞在软琼脂中可形成克隆。 1:3~1:6传代；2~3天换液1次。 91~95 A类 C6 NCI-H520 细胞 NCI-H520-物种鉴定-PUMC NCI-H520-细胞形态-PUMC RPMI 1640 (w/o Hepes) 10%FBS http://www.cellresource.cn/pdf/20080108101040.pdf 上皮样 中国医学科学院基础医学研究所基础医学细胞中心 人肺鳞癌细胞；NCI-H520 培养法（-） 基础培养基+8%DMSO+20%FBS 杨振丽，刘玉琴，Tel:65286441，69156455；Email:crcpumc@vip.163.com 贴壁生长 vimentin