Calu-3 cell Calu-3 细胞 NICR723 cell NICR723 细胞 3131C0001000700157 Base Medium+8%DMSO+20%FBS Calu-3 Calu-3 cell Cell Resource Center of Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences Class A Contacts: Songhua Chen: Cell Resources Center, Shanghai Institute of life sciences, Chinese Academy of Sciences, zip code: 200032 address: No. 320, Yueyang Road, Shanghai, 021-54920404021-54920405: Fax: 021-54920612 Email: shchen@sibs.ac.cn. Digestion for 20 minutes. Passage at 1: 2. The cells can be overgrowth in 5-6 days. Human Lung Adenocarcinoma Cell Line; Calu-3 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) qualified fetal bovine serum，0.1 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 NICR723 Negative P(19+5) The cell line (strain) of the library for research purposes only, may not be used for other purposes without permission. Users may not transfer the library cell line (strain) to a third party. Users in the publication of research papers or results, should indicate the source of cell lines (strains). The cells were isolated from the pleural fluid of a 25-year-old white male patient with adenocarcinoma of the lung who had been treated with cyclophosphamide, bleomycin and adriamycin. The cells were inoculated into nude mice for tumorigenesis and were used as transfection hosts. adherent growth epithelial cell http://www.cellresource.cn/fdetail.aspx?id=723 http://www.cellresource.cn/pdf/20080108100951.pdf A类 Calu-3 细胞 MEM-EBSS: Minimum Essential Medium (MEM Eagles with Earle＇s Balanced Salts) 优质胎牛血清，0.1 P(19+5) http://www.cellresource.cn/pdf/20080108100951.pdf 上皮细胞 中国科学院上海生命科学研究院细胞资源中心 人肺腺癌细胞；Calu-3 基础培养基+8%DMSO+20%FBS 本库的细胞系(株)仅用于研究工作，未经许可不得用于其它目的。使用者不得将本库细胞系(株)转让给第三者。使用者在发表研究论文或结果时，应注明细胞系(株)的来源。 消化20分钟。1:2。5-6天长满。 联系人：陈松华 单位：中国科学院上海生命科学研究院细胞资源中心 邮编：200032 地址：上海市岳阳路320号 电话：021-54920404，021-54920405 传真：021-54920612 Email: shchen@sibs.ac.cn 该细胞是从一名25岁的白人男性肺腺癌患者的胸水中分离建立的；该患者曾使用过环磷酰胺、博来霉素、阿霉素进行治疗。该细胞接种至裸鼠可成瘤；可作转染宿主。 贴壁生长 阴性